| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,171,885 | 0 | A | G | 39.4% | 40.3 / 14.3 | 34 | intergenic (+129/‑172) | rrfB/murB | 5S ribosomal RNA of rrnB operon/UDP‑N‑acetylenolpyruvoylglucosamine reductase, FAD‑binding |
| Reads supporting (aligned to +/- strand): ref base A (14/6); new base G (0/13); total (15/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.04e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAAC > NC_000913/4171735‑4172026 | agagTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGa < 2:574542/151‑1 (MQ=31) gTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAggg < 2:187930/151‑1 (MQ=31) tAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGt > 1:529862/1‑151 (MQ=31) ggAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGc < 1:701300/151‑1 (MQ=31) ccAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAgg < 2:525838/151‑1 (MQ=31) cAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGa < 2:788167/151‑1 (MQ=31) aGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGAc < 1:826781/151‑1 (MQ=31) aaCGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAAc > 1:543555/1‑151 (MQ=31) aaCGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAAc > 2:658289/1‑151 (MQ=31) gAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGc > 1:265404/1‑151 (MQ=31) aaaGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGcc > 1:214413/1‑151 (MQ=31) tCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGCTGCGAAGCAACGGCCCGGCGGGCGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCAt > 2:312463/1‑151 (MQ=9) acgaaaaaGTCTGGCCTTTTCGTTTTATTTGGTTTTGGGGGGTGACCGCTCTCTGGAGAAGAAAAAACCGGGGGGGGGCGGTTTTGAACTTTGGAAAGCAACGGCCGGGGGGGTGGGGGGGAGACCGCCCGCCATAAACTGCCAGGCAt < 2:531195/144‑1 (MQ=2) aGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATc > 1:184720/1‑150 (MQ=31) gTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCa > 1:419962/1‑150 (MQ=31) tCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCaa > 1:214177/1‑150 (MQ=31) aaaGCCTGGGCTTTTTTTTTTTTCTTTTTTTTGTGGGTAACCCCTCCCGTGAGAAGGAAAAACCCGCGGGGGGGGGTTTTGAAGGTTGCGAAGAAAGGGCCGGGGGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTa < 1:56611/151‑1 (MQ=2) gAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGttt > 1:397882/1‑151 (MQ=32) gCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCCGGCAGCACATTAAGCAGAAGGCCATCCTGACGGACGGCCTTTTTGCGTTTCTa > 2:277678/1‑150 (MQ=255) gCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAAGCAGCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTAc > 2:701094/1‑151 (MQ=25) ttcccgttgTAGAACAGCTCCGCCGGGGGCGCATTTGACCATTGCAACAGAACGCCCGGCAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCt < 1:30471/139‑1 (MQ=11) acaaccccgcggGGGGGGGTTTTGAACGTTGCGAAGCACCGGCCGGGGGGGGGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTgt < 1:332397/143‑1 (MQ=14) aCAAACCCGCGGGGAGGGGATTTGATCTTTGGGAAGCAACGGCCCGGGGGGGGGCGGGCAGGACCCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTgt < 1:280497/149‑1 (MQ=14) cAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTgtcg > 1:226428/1‑150 (MQ=255) atagccgcggGGCGCGGATTTGAACGTGGGAAAGCAGTGGCCGGGGGGGGGGGGGGCGGGCCCCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCAGCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTgtcg < 2:181245/145‑1 (MQ=17) aaaCCCGCGGGGGGGGAATTGGAACTTTGGAAAGCACGGGCCGGGGGGGTGGGGGGCGGGCCGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTgtcgtc < 1:543086/151‑1 (MQ=17) aaaCCCGCGGGGAGGGGTTTTGAAGGTTGCGAGCCAGCGGCCCGGGGGGGGGGGGGAAGGACCCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTgtcgtc < 2:633103/151‑1 (MQ=17) cgccgGGAGCGTATTTGAACTTTGCGAAGAACCGGCCGGGGGGGTGGCGGGCAGGCGGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTgtcgtc < 1:582227/146‑1 (MQ=17) ccgGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCt > 1:553347/1‑150 (MQ=255) gggggtggATTTAAAGGTTGGAAAGGAAGGGCGGGGGGGGGGGGGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCatat < 2:799511/140‑1 (MQ=17) ggggATTTAAACGTTGCAACGCAGCGGGCGGGGGGGGGGGGGGCAGGCCGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGcc < 2:201372/149‑1 (MQ=18) gCGGATTTGAGCGTTGGAAAGCAACGGCCCGGGGGGGGGGGGGCAGGCCCCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGcc < 1:415989/151‑1 (MQ=18) gAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACg > 2:231070/1‑151 (MQ=255) aaccagcgccccGGGGGGTGGGGGGGAGGACCCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCcaca < 2:265302/135‑1 (MQ=21) ggCCGGGGGGGGGGGGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAAc < 1:411321/149‑1 (MQ=255) | AGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAAC > NC_000913/4171735‑4172026 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |