Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,252,6460TG41.9% 65.0 / 13.3 44V316G (GTA→GGA) yeiIputative kinase
Reads supporting (aligned to +/- strand):  ref base T (6/19);  new base G (18/0);  total (25/19)
Fisher's exact test for biased strand distribution p-value = 2.28e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGGTGTTCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATG  >  NC_000913/2252502‑2252793
                                                                                                                                                 |                                                                                                                                                      
aaGGTGTTCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCATAGtgtat                                                                                                                                                    >  1:182795/1‑149 (MQ=255)
 aGGTGTTCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGc                                                                                                                                                <  1:474945/151‑1 (MQ=255)
     gtTCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTc                                                                                                                                            <  1:733482/151‑1 (MQ=255)
           cAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCTGTGTATAACGTTCtt                                                                                                                                          >  1:422216/1‑147 (MQ=255)
            aaCTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGGGTTTAGCtttttttt                                                                                                                                        >  1:686953/1‑146 (MQ=255)
                gtttgtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCGGGTGTATAGGtttttt                                                                                                                                          >  2:389471/1‑142 (MQ=255)
                   tgtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTCGGCTATAGCtttttt                                                                                                                                          >  1:703535/1‑139 (MQ=255)
                   tgtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAGAttttttt                                                                                                                                         >  2:203238/1‑139 (MQ=255)
                      ttATTTGCCCGATGAGTCAGTTTCTTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGACTGGGATATATATTTCTTTTAGGATACAGatt                                                                                                                            >  2:280518/1‑150 (MQ=255)
                       tATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTc                                                                                                                           <  1:368779/150‑1 (MQ=255)
                                gATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAACTTTTTTTTAAGGGACAGTTTTCCCGAc                                                                                                                     >  1:819462/1‑147 (MQ=255)
                                     gTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAACTTTTTTTTAGGATACAGTTTCCGCGACAGCGccc                                                                                                              >  1:703056/1‑149 (MQ=255)
                                       cAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCGGGGAGATAAATTTTTTTGAGGAAAAAGTTTTTCCGACAACCCCCCttt                                                                                                          >  2:62933/1‑151 (MQ=255)
                                       cAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGGGATATCTTTCTTTTAGGGTACAGTTTTCGCGAGAGCGCCCGgtt                                                                                                          >  1:402141/1‑151 (MQ=255)
                                        aGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTTTTGTAGGATACAGTTTTCGCGACAGCGCCCGtttt                                                                                                         >  1:856373/1‑151 (MQ=255)
                                             aTTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGt                                                                                                    <  2:23134/151‑1 (MQ=255)
                                               tGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTa                                                                                                   <  1:479203/150‑1 (MQ=255)
                                                  aGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAAATTTTTTTAAGGGTAAAGTTTTCGCGACAGCGCCCGCTTTTCGGTTGcc                                                                                                >  1:777655/1‑150 (MQ=255)
                                                    cGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTgc                                                                                             <  2:664814/151‑1 (MQ=255)
                                                     gAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATTTAGATTTTTTTAAGGATATAATTTTCGCGGCAGCGcccccctttcgggagcc                                                                                                >  2:224633/1‑133 (MQ=255)
                                                         aGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGc                                                                                       <  1:61766/151‑1 (MQ=255)
                                                            aTGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAAt                                                                                    <  1:767846/151‑1 (MQ=255)
                                                              ggAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAAttt                                                                                  <  2:288782/151‑1 (MQ=255)
                                                               gagaACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCCTGGATATAGGTTTTTTTAAGGATACAATTTTCGCGGCAGCGCCCGTTTTTTGGTAGCCCGCGGGGGCAAttt                                                                                  >  1:581752/1‑151 (MQ=255)
                                                                     aaTTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAGGTTTTTTTTAGGATACAGGTTCCGCGAGAGAGCGCCTTTTTCGGGGGCCTGCGCGGCGAGTTcctgcg                                                                            >  2:133655/1‑145 (MQ=255)
                                                                         tttGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAg                                                                        <  2:660393/150‑1 (MQ=255)
                                                                             cTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGCCCTCGGATATAGCTTTCTTTGATGATACAGGTTTCGCGCCCGCGCCCCCTTTTCGGGAGGCTGCGCGGCCAATTcgtgccc                                                                           >  2:349722/1‑141 (MQ=255)
                                                                              tGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGTTATTACTTTTTTTTAGGAGACAATTTCCGCGACAGCGCCCCGTTTTCGGGAGCCTGCGCGGCAAATTCTCCCGcc                                                                          >  1:524394/1‑143 (MQ=255)
                                                                               gACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAg                                                                  <  2:824849/150‑1 (MQ=255)
                                                                                 ccGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCtt                                                              <  2:163330/151‑1 (MQ=255)
                                                                                            cATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAAATTTTTTTGAGGATACAGTTTTCGCGGCAGCGCCCGGTTTTCGGTTGCCTGGGGGGCACTTTTTCGCGCCCGCGGGCGCCTTAACAaccc                                                     >  2:300681/1‑149 (MQ=255)
                                                                                               aCGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGTTATATGATTTTTTGAGGGTAAAGTTTTCCCGCCAGCGCCCGGTTTTTGGTAGGCCGGGCGGCCATTTTGCCCGGCCGCGGCAGCCTTAAAAAacccaccc                                                 >  1:642727/1‑147 (MQ=255)
                                                                                                 gACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAGCTTTCTTTAAGGGTAAAGTTTCCGCGGCCGCGCCCGTGTTTCGGGAGGCCGCCCGGCAAATTCCCTCGGCCGCGGCCGCCTAaaaaacacccccc                                                >  2:248000/1‑142 (MQ=255)
                                                                                                    aGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGGTATAGCATTTTTTTAGGATAAAGTTTTCGCGGCAGCGGCCGCTTTGCGGTAGCCCGCGGGGCGAATTTTCGCCCCCGCGGCAGCTTAAAAAaccctcccc                                                >  1:239317/1‑146 (MQ=255)
                                                                                                        gACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAGCTTTTTTTTAGGGTAAAAGTTTCGCGACAGCGCCCGGTTTTCGGGAGCCTGCGCGGCAATTTTGCGCGCCCGCGGCAGCTTAAAAAACCCCACCCCCTCTGcc                                        >  1:651482/1‑150 (MQ=255)
                                                                                                            gTTTTGGTGCTGACGATGGTTTTACGGCGGTCCTCGTATATAGCTTTCTTGATGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGCTAGCCTGCGCGGCAATTTCACGCGCTAGCGTCAGCCTAAACAACGCTACCCTGTCTGCCGATaa                                   <  2:714064/151‑1 (MQ=255)
                                                                                                                 ggTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAAcgcg                               <  1:604647/150‑1 (MQ=255)
                                                                                                                 ggTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACgcgc                              <  1:203296/151‑1 (MQ=255)
                                                                                                                       gACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCAt                        <  2:402880/151‑1 (MQ=255)
                                                                                                                        aCGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCAt                        <  2:422083/150‑1 (MQ=255)
                                                                                                                                     ggCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGgtg          >  1:519158/1‑151 (MQ=255)
                                                                                                                                       cGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTa        <  1:816991/151‑1 (MQ=255)
                                                                                                                                           gCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAAc      >  1:649974/1‑150 (MQ=255)
                                                                                                                                            ccTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACa     >  2:357267/1‑150 (MQ=255)
                                                                                                                                               ggTATATAGCTTGCTTGAAGGATACAGTTTCCGGGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATg  <  2:649426/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                      
AAGGTGTTCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATG  >  NC_000913/2252502‑2252793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: