Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,601,784 | 0 | A | G | 39.6% | 76.6 / 16.0 | 54 | E195G (GAG→GGG) | hyfA | hydrogenase 4, 4Fe‑4S subunit |
Reads supporting (aligned to +/- strand): ref base A (6/26); new base G (21/0); total (28/26) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.14e-09 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGC‑GGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACG‑CTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATC > NC_000913/2601639‑2601933 | gACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGC‑Ggggggg > 2:478839/1‑148 (MQ=255) tgtgtgATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGC‑GGGGGGGATcccc > 1:769734/1‑150 (MQ=255) gtgtgATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGC‑GGGGAGGATCCCCt < 1:123224/150‑1 (MQ=255) tgtgATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGC‑GGGGGGGGGTCCCttt > 1:335082/1‑151 (MQ=255) gATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGC‑GGGGAGGATCCCCTTTccc < 2:228268/151‑1 (MQ=255) ttCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGC‑GGGGGGGGGCCCCCTTccccc > 1:613208/1‑148 (MQ=255) tCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGC‑GGGGAGGGTCACCCTTcccctac > 2:857681/1‑147 (MQ=255) tCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGC‑GGGGAGGATCCCCTTTCCctca < 2:278612/150‑1 (MQ=255) tGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGC‑GGGGAGGATCCCCTTTCCctcact < 1:477534/149‑1 (MQ=255) gCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGC‑GGGGAGGATCCCCTTTCCctcactca < 2:12138/150‑1 (MQ=255) cAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGC‑GGGGAGGATCCCCTTTCCCTCACTCAGGa < 2:319163/151‑1 (MQ=255) aGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGC‑GGGGGGGGTCCCCTTTTCCTCCCTCAGGAg > 1:840314/1‑148 (MQ=255) ggTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGC‑GGGGGGGGTCCCCCTTTCctccccc > 2:297689/1‑138 (MQ=255) gTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGC‑GGGGAGGGACCCCCTTCCCTCACCCAGGGGCaa > 1:306493/1‑149 (MQ=255) tCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGGGGGCGCCCCTTTCCttcaccacggg > 1:91220/1‑139 (MQ=255) cGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGC‑GGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACG‑CTa < 1:603075/151‑1 (MQ=255) ccTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGC‑GGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACG‑CTAATg < 1:27370/151‑1 (MQ=255) cGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGC‑GGGGGGGAACCCCCTTTCCCCCCTCAGGGGCAAAG‑CTAAAGGa > 1:311832/1‑149 (MQ=255) cgcgCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGC‑GGGGGGGGTCCCCTTTCCCCCACCCCGGAGCAACG‑CTAATGGATGccc > 1:626481/1‑150 (MQ=255) gcTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGC‑GGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACG‑CTAATGGATGCCCTGCa < 1:834369/151‑1 (MQ=255) gCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGC‑GGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACG‑CTAATGGATGCCCTGCAatta < 1:738066/151‑1 (MQ=255) cccGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGC‑GGGGGGGATCCCCCTTTCCCCAATCAAGAACAACGCCTAATGGAGGCCCtcaaat > 2:741310/1‑144 (MQ=255) gAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGC‑GGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACG‑CTAATGGATGCCCTGCAATTATTaa < 2:806440/151‑1 (MQ=255) ggCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGC‑GGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACG‑CTAATGGATGCCCTGCAATTATTAACCTGGt < 2:138543/151‑1 (MQ=255) gTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGC‑GGGGGGGGGCCCCTTTCCCTCACTCACGAGGAACG‑CTCATGGACTCCCTGCCattttt > 2:346393/1‑140 (MQ=255) tACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGC‑GGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACG‑CTAATGGATGCCCTGCAATTATTAACCTGGTCGCt < 2:554000/150‑1 (MQ=255) aCGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGC‑GGGGGGGGTCCCCCTTCCCTCCCCTAGGGGCCACC‑CTAATAGATGGCCCGGAATTATTTACCt > 1:164325/1‑142 (MQ=255) aCGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGC‑GGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACG‑CTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGa < 1:576326/151‑1 (MQ=255) cGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGC‑GGGGAGGAACCCCTTTCCCCCACTCCGGAGCAAAG‑CTAATGGAAGCCCTGCCATTATTAACCTGGTCGCTg > 1:271208/1‑149 (MQ=255) ggCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGC‑GGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACG‑CTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGAtt < 2:131852/151‑1 (MQ=255) tGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGC‑GGGGGGGGTCCCCTTTTCCTCACTCAGGGGCAAAG‑CTAATGGGTGGCCCGCAATTATTAACCTGGTCGCTGAtaga > 1:29992/1‑147 (MQ=255) tGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGC‑GGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACG‑CTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGAt < 2:29988/147‑1 (MQ=255) aTCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGC‑GGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACG‑CTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTa < 2:259695/151‑1 (MQ=255) ccGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGC‑GGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACG‑CTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTg < 2:409964/151‑1 (MQ=255) cGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGC‑GGGGGGGGACCCCCTTCCCCCCCCCAGGAGGAACC‑CCAAAGGGTGGCCCGCCATTATTTAACTGGTCGCTGATTTTCTATc > 1:4058/1‑148 (MQ=255) cGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGC‑GGGGAGGATACCCTTTCCCCCCCTCAGGGGCCACG‑CTCATGGATGGCCTGCAATTATTAACATCGGCGCTGAGTTTCTATCTGt > 2:97815/1‑151 (MQ=255) aTAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGC‑GGGGCGGGACCCCCTTTCCTCACCCACGAGCAACC‑CTAAAGGATGGCCCCCAATTATTCACATCGTCGCTGAATTTCTATTTCTTTGCt > 2:469013/1‑151 (MQ=255) aGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGC‑GGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACG‑CTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAg < 2:541032/151‑1 (MQ=255) cTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGGGGGACCCCCTTTCCC‑CACTCAGGGGCAACC‑CTAATGGGGGCCCCGCAATTATTTACCTGGGCGCTGGTTCTCTCTCTGTTTGCtcattt > 2:691330/1‑146 (MQ=255) gCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGC‑GGGGAGGAACCCCCTTCCCCCACTCAGGAGCACCA‑CGAATGGATGCCCTGCAATTATTTACCTGGGCGCTGATTCTCTATTTGTTTTCTTGTCt > 2:243194/1‑149 (MQ=255) gTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGC‑GGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACG‑CTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCg < 2:220740/151‑1 (MQ=255) cAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGC‑GGGGGGGGTCCCCCTTTCCCCACTCAAGAGCAACG‑CTAATGGATGCCCCGCAATTATTAAACTGGTCGCTGGTTTTCTTTCTGTTTTCTAGTCTGGCtt > 1:59669/1‑147 (MQ=255) gaaagaaaAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGC‑GGGGAGGAACCCCCTTCCCTCACTCCGGAGCAACG‑CTAATGGGTGCCCTGCAATTATTTAACCGGGCGCCGGTTTTCTATTTGTTTTCTAGTCTGGGTTCGCTGtttt > 1:852135/1‑151 (MQ=255) aaaaCAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGC‑GGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACG‑CTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCg < 1:659554/151‑1 (MQ=255) aCAGCGCCTGGCCGCCAGCTGGCTTGCCAATGGC‑GGGGAGGATCCCCTTTCCCTCCCTCAGGAGCACCG‑CTAATGGCTGCCCCGCTATTATTAACCTGTTCGCCGAGTCTCTATCTGTTTGCTAGTTTGGCTTCGCTGTTTTTACTCGGt < 2:122714/150‑1 (MQ=255) cgcCTTGCCGCAAGCTGGTTTGCCAATGGC‑GGGGGGGTTCCCCCTTCCCCCCCTCCCGAGCAACA‑CCACAGGGTGCCCTGCAATTTTTTCCACTGTCGCCGGTTCTCTCTCTGTTTGCTTGTGTGGGTTCGCTGctttttttttttctggg > 2:102951/1‑139 (MQ=255) ccTTGCCGCAAGCTGGTTTGCCAATGGC‑GGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACG‑CTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACa < 2:840053/151‑1 (MQ=255) tGCCGCAAGCTGGTTTGCCAATGGC‑GGGGGGGGTCCCCCTTTCCTCACCCCGGGGCAAAG‑CCAATGGATGCCCTCCCCTTTTTTATCTTGTCGCTGATTTTCTATCTTTTTTCTTGTCTGGGTTCGCTGTTTTTAtttggtttgg > 2:630973/1‑145 (MQ=255) tGCCGCAAGCTGGTTTGCCAATGGC‑GGGGGGGAACCCCTTTTCCTCACTCCGGCGGAACC‑CTAATGGGTGGCCTGCAATTATTTACCTGGGCGCTGCTTCTTTTTTTGGTTGTTAGTTTTGCTTCTCTGTTTTTTCTTGGTCTGGTCAg > 2:332587/1‑149 (MQ=255) gCCGCAAGCTGGTTTGCCAATGGC‑GGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACG‑CTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGAc < 2:134824/150‑1 (MQ=255) gCAAGCTGGTTTGCCAATGGC‑GGGGGGGAACCCCTTTTCCTCCCTCAGGAGCAAAG‑CCAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTTTCTTTTTTTTTGCTAGTCTGGCTTCGCTGTTTTTTCTCGGGCTgggaagaacgg > 1:828316/1‑141 (MQ=255) aaGCTGGTTTGCCAATGGC‑GGGGGGGGTCCCCTTTTCCTCCCTCAGGGGCAACG‑CTAATGGGTGCCCCGCAATTATTAACCTGGTCGCTGATTTTCTATCTGTTTGCTAGTCTGGCTTTGCTGTTTTTTCTCGGTCTGGGCAGACTgg > 1:184203/1‑148 (MQ=255) aaGCTGGTTTGCCAATGGC‑GGGGGGGGGCCCCTTTTCCTCAATCAAGAACAACG‑CCAATGGGTGCCCCTCGAATTTTAAACTGGTGGCGGTATTTCTCTCTGTTTGTTAGTCCGGCTTCGCTGTTTTTATCCGGTCTGGGCAGACCGGCt > 2:398546/1‑150 (MQ=255) ttGCCAATGGC‑GGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACG‑CTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCt < 1:587782/150‑1 (MQ=255) cAATGGC‑GGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACG‑CTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCg < 2:858205/151‑1 (MQ=255) ggaggaTCCCCTTTCCCTCACTCAGGAGCACCG‑GTAATGGATGCCCTGCAAGTATTAGCCTGCTCGGTGATTCTCTATCTGTTTGGTAGTCTGGCATCGCTGTTTTTACTCGGTCTGGACAGAGTTGCTGTTAAGCCTTCCGGCATcaca < 2:409942/150‑1 (MQ=255) gaggaTCCCCTTTCCCTCACTCAGGAGCAACG‑CTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATc < 1:844157/151‑1 (MQ=255) | GACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGC‑GGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACG‑CTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATC > NC_000913/2601639‑2601933 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |