Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,001,9180AC49.1% 91.5 / 11.9 59intergenic (‑129/+192)fliA/fliCRNA polymerase, sigma 28 (sigma F) factor/flagellar filament structural protein (flagellin)
Reads supporting (aligned to +/- strand):  ref base A (25/5);  new base C (1/28);  total (26/33)
Fisher's exact test for biased strand distribution p-value = 1.23e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGC  >  NC_000913/2001768‑2002066
                                                                                                                                                       |                                                                                                                                                    
cAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGaaaaaaa                                                                                                                                                      <  2:513116/151‑1 (MQ=255)
   cGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAAccc                                                                                                                                                   <  1:22372/151‑1 (MQ=255)
     gTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCccg                                                                                                                                                 <  1:767603/151‑1 (MQ=255)
         agagTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCgg                                                                                                                                             >  1:559566/1‑151 (MQ=255)
            gTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTgg                                                                                                                                          >  1:646207/1‑151 (MQ=255)
             tGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAAGGAGGGGTTATTTGGGGTTTACAGGTAAATTCCAGGCAAAAAAAACCCCCGCCGGTgg                                                                                                                                          <  2:108696/150‑1 (MQ=255)
               atttCACGATAAACAGCCCTGCTTTATATGAGTTAGCGGCATGATTATCCGTTTCTGCGGGTTTTTTAATCGGACGATTAGTGGGTGAAAGGAGGGGTTATTTGGGGGTTACAGATAAATTCCAGGCAGAAAAAAACCCCGCCGGTgg                                                                                                                                          <  2:554090/146‑1 (MQ=255)
               aaTTCACGATAAACAGCCCTGGTTTATATGAGTTTTCGGTATGATTTCCCGTTTCTGCAGGTTTTTTAACCGGACGATTAGGGGGTAAAAGGAGGGGTTTTTTGGGGGTTAAGGGTAAATCCCAGGCAAAAAAAACCCCCGCCGGTGGc                                                                                                                                         <  2:478183/149‑1 (MQ=255)
               aaTTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCgg                                                                                                                                       >  1:691342/1‑151 (MQ=255)
                 ttCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGTTTTTTAAGCGGACGATTAGTGGGTAAAATGGGGGGTTATTTGGGGTTTACAGGTAAATTCCAGGCAGAAAAAACCCCCGCCGGTGGCggg                                                                                                                                      <  2:512306/150‑1 (MQ=255)
                       ctAAACAGCCCTGCGTTATATGAGTTCTCGGCATGATTATCCGTTTCTCCCGGGCTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGCGGCGGGGAAGCAc                                                                                                                               >  2:128743/2‑151 (MQ=255)
                       aTAAACAGCCCTGCTTTATATGATTTATCGGAATGTTTATCCTTTTCTGCGGGTTTTTAAATCGGACGATTAGTGGGTGAAATGGGGGTTTATTGGGGGGTTACAGGTAAATTCCAGGCAGAAAAAACCCCCGCCGGTGGCGGGGAAGCa                                                                                                                                <  1:788759/150‑1 (MQ=255)
                               ctttGCTTTATAGGAGTTATGGGTATGTTTTCCCGTTTCTGCGGTTTTTTTATTCTCACGTTTAGGGGATAAAAGGGGGGGTTTTTTGGGGGTTACAGTTAAATCCCGGGAAAAAAAACCCCCCGCCGGTGGCGGGGAAGCACGtt                                                                                                                            <  2:606274/143‑1 (MQ=255)
                                ccggctttATAGGGGTTAGCGGCAGGATTATCCGTTGCTGGGGGGTTTTTAAGCGGACGAGTAGGGGGTGAAAGGGGGGGTTTTTTGGGGGTGACGGGAAAATGCCGGGCAGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGAc                                                                                                                      <  2:289250/145‑1 (MQ=255)
                                ccTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCt                                                                                                                         >  1:606565/1‑148 (MQ=255)
                                 cTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTGGGGGTTTACAGGTAATTTCAAGGCGGAAAAAACCCCCGCGGGTGGCGGGGAAGccgg                                                                                                                              >  2:232764/1‑139 (MQ=255)
                                   gctttATATGAGTTACCGGCATGATTATCCGTTTCGGCGGGGTTTTTAACGGGACGATTAGGGGGGAAAAGGAGGGTTTATTTGGGGTTTACAGGTAAATTCCAGCAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACaaa                                                                                                                   <  1:498259/148‑1 (MQ=255)
                                      ttATATGGTTTACCGGCATGTTTATCCTTTTCGGCGGGTTTTTTAATCGGACGTTTAGGGGGTAAAAGGGGGGTTTATTGGGGGGTTAAAGGAAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAAt                                                                                                                  <  1:390525/149‑1 (MQ=255)
                                        atatGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTgcg                                                                                                              >  1:808265/1‑151 (MQ=255)
                                        atatGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTgcg                                                                                                              >  1:719236/1‑151 (MQ=255)
                                             aGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCttt                                                                                                          >  2:39816/1‑150 (MQ=255)
                                               ttATCGGCATGATTATCCGTTTCTGGAGGGTTTTTAATCGGACGATTAGTGGGGGAAATGAGGGGTTTTTTGGGGGTTACAGGAAAATTCCAGGAAAAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTAt                                                                                                        <  2:671080/150‑1 (MQ=255)
                                                    ggCATGATTATCCGTTTCTGGAGGTTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGTTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGc                                                                                                   <  1:128781/150‑1 (MQ=255)
                                                    gccATGATTTTCCTTTTGCGCGGGGTTTTAAGTCGGAAGATTGGTGGGGGAAAGGAGGGTTTATTTGGGGGTGAAAGTAAAATTCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTa                                                                                                         <  2:431523/142‑1 (MQ=255)
                                                         gATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATg                                                                                             >  1:852400/1‑151 (MQ=255)
                                                               ccGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGt                                                                                       >  1:470746/1‑151 (MQ=255)
                                                                cGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGt                                                                                       >  1:185166/1‑150 (MQ=255)
                                                                        cAGGGTTTTTAATCGGACGATTAGTGGGTGACATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCtt                                                                              >  2:531740/1‑151 (MQ=255)
                                                                         aGGTTTTTTAATCGGCCGATTAGTGGGTGAAATGAGGGTTTTTTTGGGGGTTAAAGTTAAATTCCAGGCAGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACg                                                                                  <  2:32764/146‑1 (MQ=255)
                                                                         aGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCtt                                                                              >  1:468347/1‑150 (MQ=255)
                                                                          ggttttttAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGAAAATTCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTAt                                                                            <  1:636923/148‑1 (MQ=255)
                                                                             tttttAATCGGACGATTAGGGGGTAAAATGGGGGGTTATTTGGGGTTTACGGGTAAATCCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTAt                                                                            <  1:842837/148‑1 (MQ=255)
                                                                                  aagcGGAGGTTTGGTGGGTGAAATGAGGGTTTTTTGGGGGTTTACAGGTAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCt                                                                     <  1:556595/147‑1 (MQ=37)
                                                                                  aaTCGGACGTTTAGGGGGTGAAATGAGGGTTTATTTGGGGGTTACAGGTAAATTCCAGGAAGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTa                                                                    <  1:208728/151‑1 (MQ=255)
                                                                                    tCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAc                                                                   >  2:263115/1‑150 (MQ=255)
                                                                                      ggACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaa                                                                 >  2:560293/1‑150 (MQ=255)
                                                                                       gACGATTGGTGGGTAAAATGGGGGTTTTTTTGGGGGTTACGGGTAATTTCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaa                                                               <  1:519591/151‑1 (MQ=37)
                                                                                        aCGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaa                                                               >  2:882384/1‑150 (MQ=255)
                                                                                            ttggggggaaaaaTGGGGGTTTATTTGGGGGTTACAGGAAAATTCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAtgt                                                           <  1:576517/140‑1 (MQ=37)
                                                                                             tAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGc                                                         >  1:28059/1‑151 (MQ=255)
                                                                                              aGTGGGTGAAATGAGGCGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGc                                                         >  2:499460/1‑150 (MQ=255)
                                                                                                tGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCaa                                                       >  1:361027/1‑150 (MQ=255)
                                                                                                 gggTGAAATGAGGGGTTTTTTGGGGGTTACAGAAAAATCCCGGGAAGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCaa                                                       <  2:895755/149‑1 (MQ=38)
                                                                                                 gggTAAAATGAGGGTTTTTTTGGGGGTTACAGGTAAATCCCAGCAAGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAtgtg                                                          <  2:358715/146‑1 (MQ=38)
                                                                                                      aaagggggggTTTTTTGGGGGTTACAGGAAAATTCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTc                                                   <  1:437760/142‑1 (MQ=37)
                                                                                                      aaaTGGGGGGTTTTTGGGGGTTTACAGGTAAATCCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTc                                                   <  1:812663/148‑1 (MQ=37)
                                                                                                       aaTGAGGGTTTATTGGGGGGTTACAGGTAAATTCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATa                                               <  1:17320/151‑1 (MQ=255)
                                                                                                           aGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAAtt                                           >  1:421139/1‑151 (MQ=255)
                                                                                                            ggggTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTg                                          >  2:511711/1‑151 (MQ=255)
                                                                                                             gggTTTTTTTGGGGGTAAGGGTAAATTCCAAGCAAAAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTg                                          <  2:276533/151‑1 (MQ=25)
                                                                                                             gggTTATTTGGGGGTTACAGGAAAATTCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGc                                         <  1:183240/151‑1 (MQ=255)
                                                                                                              gttttttggggggTTACAGGTAAATTCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCa                                        <  1:851399/143‑1 (MQ=255)
                                                                                                                   tttGGGGGTTAAAGTAAAATCCAAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGTCAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAAtt                                     <  2:630610/149‑1 (MQ=25)
                                                                                                                    ttGGGGGTTACAGGTAAATTCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAAtt                                     <  2:40964/148‑1 (MQ=255)
                                                                                                                     tGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAAc                                 >  1:359632/1‑151 (MQ=255)
                                                                                                                         ggTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGt                             >  1:455542/1‑151 (MQ=255)
                                                                                                                            tACAGGTAAATCCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAg                           <  2:321659/150‑1 (MQ=255)
                                                                                                                                   aaaTCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAgg                          <  1:321267/144‑1 (MQ=255)
                                                                                                                                    aaTTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATa                   >  2:265638/1‑150 (MQ=255)
                                                                                                                                      ttCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagc                >  1:357322/1‑151 (MQ=255)
                                                                                                                                                    aaaaCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAgg   >  1:454686/1‑151 (MQ=255)
                                                                                                                                                     aaaCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGc  >  2:115441/1‑151 (MQ=255)
                                                                                                                                                       |                                                                                                                                                    
CAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGC  >  NC_000913/2001768‑2002066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: