Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,508,662 | 0 | T | A | 40.4% | 81.1 / 19.5 | 57 | E384D (GAA→GAT) | yhfW | phosphopentomutase‑related metalloenzyme superfamily protein |
Reads supporting (aligned to +/- strand): ref base T (10/24); new base A (23/0); total (33/24) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.86e-08 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGC‑GGAAAAATTC‑ACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGCCATC > NC_000913/3508520‑3508809 | tttttAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGC‑GGGAAAAATt‑acaca > 1:396347/1‑150 (MQ=255) ttttAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGC‑GGAAAAATTC‑acacac < 1:772779/150‑1 (MQ=255) tttAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGC‑GGAAAAATTC‑ACACACg < 1:42448/150‑1 (MQ=255) ttAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGGGGGGCGC‑GGGAAAAATC‑ACACACgg > 1:909877/1‑150 (MQ=255) aaaGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGGGCGGGAAAAAttaaa > 1:819816/1‑140 (MQ=255) aaGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGC‑GGAAAAATTC‑ACACACGGTAGc < 2:674093/151‑1 (MQ=255) aGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGGGC‑GGGAAAAATC‑AAAAACAGTAGcc > 2:76287/1‑151 (MQ=255) aGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGG‑GGAAAAAATC‑ACACACGGGAg > 2:646812/1‑149 (MQ=255) aGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATTA‑AAAAACggaacc > 2:92727/1‑147 (MQ=255) gCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTTGCGCGG‑GGGAAAAATC‑ACACAACGTAGacc > 1:567696/1‑151 (MQ=255) agagaTCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGC‑GGAAAAATTC‑ACACACGGTAGCCCCcaca < 1:664959/151‑1 (MQ=255) aaTATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGC‑GGGAAAAATC‑AAACACCGGAGCCCCCCCACCCGaa > 2:924704/1‑150 (MQ=255) atatGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTCGCGCGC‑GGGAAAAATC‑ACCCACAGTAGCCCCCCCAt > 1:147950/1‑144 (MQ=255) atGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGC‑GGAAAAATTC‑ACACACGGTAGCCCCCACATCAGAAAGc < 1:415142/150‑1 (MQ=255) tGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGC‑GGGAAAAATC‑ACACACCGGAGCCCCCCCATCAGaa > 1:147772/1‑146 (MQ=255) aGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGG‑GGGAAAAATT‑ACACACGGTAGCCCCCCCAACCGAAAACgcgg > 2:819102/1‑150 (MQ=255) aGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGC‑GGAGAAATTA‑ACAAACGGGAACCCCCCCCTCGGaagga > 2:84910/1‑143 (MQ=255) aTGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGC‑GGAAAAAATCTACAAACGG‑AGACCCCCCAACAGAAAACggcgtg > 2:490819/1‑145 (MQ=255) gggCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGC‑GGAAAAATTC‑ACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCg < 2:767046/151‑1 (MQ=255) ggCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGC‑GGAAAAATTC‑ACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGa < 1:65362/151‑1 (MQ=255) cGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGC‑GGAAAAAATC‑ACACACGGTAGCCCCCACATCAGAAAACGTGGGGGGCACAcc > 1:82253/1‑147 (MQ=255) aggtgggaaCCCGTCGGATCGAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGAAGAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGC‑GTAAAAATTC‑ACACACGGTGGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAg < 2:523647/141‑1 (MQ=255) aGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTTGCGCGC‑GGAAAAATTT‑ACACACGATAGGCCCCACAACATAAAGCGTGGTGCGCACAACCAGCTg > 1:218748/1‑151 (MQ=255) aGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGC‑GGAAAAATTC‑ACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTg < 2:353891/151‑1 (MQ=255) aGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGAGCGC‑GGAAAAAATT‑CCACACAGGAACCCCCCCAACAGAAAACgcgggggggccacc > 2:502301/1‑135 (MQ=255) gggTTTAACCCGTCGGATAAAAACTCAGAGGGTGTCCCCGGAAAACCGGAGGAAGGAAAGAAAAGACGCACCATTTTGTGGCGGGGGCGCGC‑GAAAAAATTC‑ACACACGGTAGCCCCCACATCAGAAAGGGTGGGGCGCACACCGaatt < 2:159459/149‑4 (MQ=255) tgtAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGC‑GGGAAAAATC‑ACACACCCAAGGCCCCCCCTCCGAAAGCGTGGGGGGGCCACCGCGCTGCggg > 2:532146/1‑149 (MQ=255) tgtAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGC‑GGGAAAAATT‑ACACACGGTAGCCCCCCCCTCAGAAAGCGGGGGGGGCACACCGAGCTGCg > 1:204894/1‑149 (MQ=255) gtAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGC‑GGCAAAATTC‑CCACACGGTAGCCCCCCCATCAGAAAGCGGGGTGCGCACAcc > 1:160142/1‑140 (MQ=255) aaCCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGGGGGGCGC‑GGGAAAAATT‑ACACAAGGTCGCCCCCCCATCCGAAAGCGGGGTGCGCACACCCAGCTGCg > 2:565603/1‑146 (MQ=255) cccGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGC‑GGAAAAATTC‑ACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGat < 2:155161/151‑1 (MQ=255) cAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGC‑GGAAAAATTC‑ACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGc < 1:101170/151‑1 (MQ=255) cTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGGGGCGCGC‑GGAAAAAATC‑ACACACGGGAGCCCCCACAACAGAAAACGGGGGGCGCACACCCAGCTGCGGAGCGGTCATCCCCTGCTGAt > 1:577924/1‑150 (MQ=255) aTAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGC‑GGAAAAAATC‑ACAAACGGGAGCCCCCACCTCAGAAAGAGGGGTGGGCACACCGCACTGCGTAGTGGTCATCCCCTGCTGGTaaa > 2:881678/1‑150 (MQ=255) gggTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGC‑GGGAAAAATT‑ACACACGGGAGACCCCCCACCAGACAGAGAGGGGGGCGCACCCAGCTGCGGAGCGAGTATCCCTCGCTGTTaaaac > 2:541741/1‑147 (MQ=255) gtgtCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTTGGGCGG‑GGGAAAATTT‑ACACAACGGAGGCCCCCCACCAAGAAACGAGGGGGGCACACCGCGCCGCGGCGGGGTCAACCCCTTCTGGTAAAACAGc > 1:384128/1‑150 (MQ=255) gtCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGC‑GGAAAAATTC‑ACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACt < 2:336918/151‑1 (MQ=255) tGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGAGCGG‑GGGAAAAATA‑AAAAACCGGAAACCCCCCACCAGAAAGCGGGGGGGGCACACCGCGCTGCGGAGCGATCATCCCCTGCTGATAAAACAACACCGGcacc > 2:266205/1‑151 (MQ=255) gCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGC‑GGAAAAATTC‑ACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGcacca < 1:464029/151‑1 (MQ=255) ccGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGC‑GGAAAAATTC‑ACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTcg < 2:398387/151‑1 (MQ=255) ggagggagGAAAGAAAAGAGCGACCATTTTGTGGCGGTTGCGCGC‑GGGAAAATTC‑ACACCCGGTAGACCCCCCATCAGAACGCGGGGGGCGGACACCGCGCTGCGGAGCGATCCTCCCCTTCTGATAAAACAGCACAGGCACCACCTcgcg > 2:212849/1‑151 (MQ=255) ggagggagGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGC‑GGGAAAAATC‑ACACACGGTAGCCCCCCCATCAGAAAACGTGGGGGGCACACCGAGCCGCGGAGCGATCATCCCCTGCTGATAAAACAGCACTGGCACCACTTcgcg > 1:268388/1‑151 (MQ=255) agggaggAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGC‑GGGAAAATTT‑ACACACCGGAGCCCCCCCCTCAGAAAGCGGGGGGCGCCCAACCGGCCGCGTAGCGATCATCCCCTGCTGATAAACCAGCACTGGCACCACCTCCCggg > 2:875681/1‑151 (MQ=255) aggaAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGC‑GGAAAAATTC‑ACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTAtg < 1:21741/151‑1 (MQ=255) aaagaaaagaGCGACCATTTTGTGGCGGTGGCGCGC‑GGGAAAAATC‑ACACACGGTAGGCCCCCCATCAGAAAACGGGGGGCGCACCCCGCGCCGCGTAGCGATCATCCCTTTCTGATAAAACAACACCGGCCCCCCCTCTCGGGGAtgggg > 2:937443/1‑149 (MQ=255) aagaGCGACCATTTTGTGGCGGTGGGGCGC‑GGGAAAAATT‑AAACACCGTAACCCCCACATCAGAAAGCGGGGGGCGCACACCGAGCTGCGTGGCGATCATCCCTTTCTGTTAAACCAGCACTGGCACCCCTTTGCGGGGATggtgggtg > 1:247705/1‑149 (MQ=255) aCCATTTTGTGGCGGTGGCGCGC‑GGAAAAATTC‑ACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATg < 1:153239/151‑1 (MQ=255) ccATTTTGTGGCGGTGGCGCGC‑CGGAAAAATC‑ACAAACCGGAGCCCCCCCATCAGAAAGCGTGGTGGGCACACCGAGCCGCGGCGAGGTCATCCCCTGCTGATAAAACAACACTGGCACCACCTCGCGGGGATGGGGGGGGTGACCAAAgg > 2:882837/1‑151 (MQ=255) gtggcggtggcgCGC‑GGAAAAATTC‑ACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGAt < 1:490091/150‑1 (MQ=255) tggcggtggcgCGC‑GGAAAAATTC‑ACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCg > 1:878800/1‑151 (MQ=255) ggcggtggcgCGC‑GGAAAAATTC‑ACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCg < 2:540744/150‑1 (MQ=255) ggtggcgCGC‑GGAAAAATTC‑ACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGc > 1:284179/1‑151 (MQ=255) ggtggcgCGC‑GGAAAAATTC‑ACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGc > 2:355865/1‑151 (MQ=255) gtggcgCGC‑GGAAAAATTC‑ACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGcc < 2:564196/151‑1 (MQ=255) cgcgc‑gGAAAAATTC‑ACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGa < 1:239758/151‑1 (MQ=255) gc‑gGAAAAATTC‑ACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATcc < 1:496878/151‑1 (MQ=255) gc‑gGAAAAATTC‑ACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATcc < 1:608495/151‑1 (MQ=255) aaaTTC‑ACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCTTTGCCGTGATCCGCCATc < 2:789979/151‑1 (MQ=255) | TTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGC‑GGAAAAATTC‑ACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGCCATC > NC_000913/3508520‑3508809 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |