Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,141,832 A→T D128E (GAT→GAA)  dcd ← deoxycytidine triphosphate deaminase; dCTP deaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,141,8320AT97.8% 158.7 / NA 45D128E (GAT→GAA) dcddeoxycytidine triphosphate deaminase; dCTP deaminase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (24/20);  minor base C (1/0);  total (25/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

CGCATCTTCACGGCGGTTGTAAGGTCGCACCGCCGGGCCGGAAAGCGGCTCAAAGCTCAGCGCACCAATTAACATGCCCGGACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGC  >  NC_000913/2141685‑2141978
                                                                                                                                                   |                                                                                                                                                  
cGCATCTTCACGGCGGTTGTAAGGTCGCACCGCCGGGCCGGAAAGCGGCTCAAAGCTCAGCGCACCAATTAACATGCCCGGACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGTTCg                                                                                                                                                 <  1:845072/151‑1 (MQ=255)
          cggcggTTGTAAGGTCGCACCGCCGGGCCGGAAAGCGGCTCAAAGCTCAGCGCACCAATTAACATGCCCGGACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGTTCGATGCGGTgcg                                                                                                                                       <  1:940349/151‑1 (MQ=255)
           ggcggTTGTAAGGTCGCACCGCCGGGCCGGAAAGCGGCTCAAAGCTCAGCGCACCAATTAACATGCCCGGACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGTTCGATGCGGTgcgc                                                                                                                                      >  1:100018/1‑151 (MQ=255)
            gcggTTGTAAGGTCGCACCGCCGGGCCGGAAAGCGGCTCAAAGCTCAGCGCACCAATTAACATGCCCGGACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGTTCGATGCGGTGcgcg                                                                                                                                     >  1:870187/1‑151 (MQ=255)
                ttGTAAGGTCGCACCGCCGGGCCGGAAAGCGGCTCAAAGCTCAGCGCACCAATTAACATGCCCGGACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGTTCGATGCGGTGCGCGGTg                                                                                                                                  >  1:918933/1‑150 (MQ=255)
                ttGTAAGGTCGCACCGCCGGGCCGGAAAGCGGCTCAAAGCTCAGCGCACCAATTAACATGCCCGGACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGTTCGATGCGGTGCGCGGTg                                                                                                                                  >  2:123479/1‑150 (MQ=255)
                  gTAAGTTCGCACCGCCGGGCCGGAAAGCGGCTCAAAGCTCAGCGCACCAATTAACATGCCCGGACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGTTCGATGCGGTGCGCGGTGACg                                                                                                                               <  2:755774/151‑1 (MQ=255)
                   tAAGGTCGCACCGCCGGGCCGGAAAGCGGCTCAAAGCTCAGCGCACCAATCAACATGCCCGGACGCCGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACCCCACCACAATGCAACCAGACCAGCCCGGTTCGATGCGGTGCGCGCTg                                                                                                                                  >  2:309061/1‑147 (MQ=255)
                     aGGTCGCACCGCCGGGCCGGAAAGCGGCTCAAAGCTCAGCGCACCAATTAACATGCCCGGACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGTTCGATGCGGTGCGCGGTGACGt                                                                                                                              <  1:762720/149‑1 (MQ=255)
                      ggTCGCACCGCCGGGCCGGAAAGCGGCTCAAAGCTCAGCGCACCAATTAACATGCCCGGACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGTTCGATGCGGTGCGCGGTGACGTGCa                                                                                                                           <  2:759953/151‑1 (MQ=255)
                           cACCGCCGGGCCGGAAAGCGGCTCAAAGCTCAGCGCACCAATTAACATGCCCGGACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGTTCGATGCGGTGCGCGGTGACGTGCACCATc                                                                                                                      >  2:241711/1‑151 (MQ=255)
                                  gggccggAAAGCGGCTCAAAGCTCAGCGCACCAATTAACATGCCCGGACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGTTCGATGCGGTGCGCGGTGACGTGCACCATCAGcccc                                                                                                                >  1:326094/1‑150 (MQ=255)
                                       ggAAAGCGGCTCAAAGCTCAGCGCACCAATTAACATGCCCGGACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGTTCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGAcgc                                                                                                          >  1:378702/1‑151 (MQ=255)
                                        gAAAGCGGATCAAAGCTCAGCGCACCAATTAACATGCCCGGACGCAGGGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGTTCGATGCGATGCGCGGGGGCGAGCACCATCAGCCCCAGAcgc                                                                                                          >  2:148655/1‑150 (MQ=255)
                                                     aGCTCAGCGCACCAATTAACATGCCCGGACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGTTCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACg                                                                                            >  1:780839/1‑151 (MQ=255)
                                                        tCAGCGCACCAATTAACATGCCCGGACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGTTCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACg                                                                                            >  2:579307/1‑148 (MQ=255)
                                                                 cAATTAACATGCCCGGACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGTTCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTccagcc                                                                                 <  2:1030788/150‑1 (MQ=255)
                                                                      aaCATGCCCGGACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGTTCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGccc                                                                            >  1:322965/1‑150 (MQ=255)
                                                                       aCATGCCCGGACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGTTCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCccac                                                                          <  1:632441/151‑1 (MQ=255)
                                                                               ggACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGTTCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCccacc                                                                         >  1:487292/1‑144 (MQ=255)
                                                                               ggACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGTTCGATGCGGGGCGCGGTGACGCGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCccacc                                                                         >  2:542887/1‑144 (MQ=255)
                                                                                gACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGTTCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGc                                                                 <  2:156655/151‑1 (MQ=255)
                                                                                gACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGTTCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGc                                                                 <  1:594737/151‑1 (MQ=255)
                                                                                    cAGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGTTCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGc                                                             >  1:193265/1‑151 (MQ=255)
                                                                                      gcgcCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGTTCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCa                                                            <  2:543834/150‑1 (MQ=255)
                                                                                                 aGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGTTCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACt                                                >  1:1065059/1‑151 (MQ=255)
                                                                                                    ttACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGTTCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTcc                                              >  2:873944/1‑150 (MQ=255)
                                                                                                          gAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGTTCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCa                                       <  1:857156/151‑1 (MQ=255)
                                                                                                           aGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGTTCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCAc                                      >  2:516515/1‑151 (MQ=255)
                                                                                                                   aaCTCCAGCACAATGCAACCAGACCAGCCCGGTTCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACcgcccgcg                              >  2:567976/1‑151 (MQ=255)
                                                                                                                   aaCTCCAGCACAATGCAACCAGACCAGCCCGGTTCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACcgccagc                               >  1:916675/1‑150 (MQ=255)
                                                                                                                   aaCTCCAGCACAATGCAACCAGACCAGCCCGGTTCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACcgccagc                               <  1:688324/150‑1 (MQ=255)
                                                                                                                     cTCCAGCACAATGCAACCAGCCCAGCCCGGTCCGAGGCGGTGCGCGGTCACGTGCCCCATCAGCCCCAGACGCGCCAGTGGGGAACGCCCGTCCAGCCAGCCCGCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGGCGCcgccagc                               <  2:671235/148‑1 (MQ=255)
                                                                                                                       ccAGCACAATGCAACCAGACCAGCCCGGTTCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACcgccagcgcca                           <  1:1006318/150‑1 (MQ=255)
                                                                                                                       ccAGCACAATGCAACCAGACCAGCCCGGTTCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACcgccagcgcca                           <  2:869890/150‑1 (MQ=255)
                                                                                                                        cAGCACAATGCAACCAGACCAGCCCGTCTCGATACGGTGCGCGGTGACGTGCACCATCAGGCCCAGACGAGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCCGACCAGCTGGCGGCGCCCCCGGATCCACCGTCACctccagggcc                            >  2:254504/1‑148 (MQ=255)
                                                                                                                           cacaGTGCAACCAGCCCAGCCCGGTTCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGctc                       <  2:916274/150‑1 (MQ=255)
                                                                                                                           cacaATGCAACCAGACCAGCCCGGTTCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGctct                      >  1:889928/1‑151 (MQ=255)
                                                                                                                            acaATGCAACCAGACCAGCCCGGTTCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCtctc                     <  1:540279/151‑1 (MQ=255)
                                                                                                                                      cagaccagCCCGGTTCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGa             >  1:132567/1‑149 (MQ=255)
                                                                                                                                        gaccagCCCGGTTCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGAt         <  2:528861/151‑1 (MQ=255)
                                                                                                                                         cccagCCCGGTTCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATa        <  2:387567/150‑1 (MQ=255)
                                                                                                                                          ccagCCCGGTTCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCCCCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGAt         <  2:984604/149‑1 (MQ=255)
                                                                                                                                             gCCCGGTTCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAAc    >  1:426286/1‑151 (MQ=255)
                                                                                                                                               ccGGTTCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGc  >  1:153531/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGCATCTTCACGGCGGTTGTAAGGTCGCACCGCCGGGCCGGAAAGCGGCTCAAAGCTCAGCGCACCAATTAACATGCCCGGACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGC  >  NC_000913/2141685‑2141978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑