Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,440,924	C→G	G36R (GGC→CGC)	rpoA ←	RNA polymerase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,440,924	0	C	G	100.0%	306.7 / NA	81	G36R (GGC→CGC)	rpoA	RNA polymerase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (43/38);  total (43/38)

GGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCT  >  NC_000913/3440777‑3441071                                                                                                                                                    |                                                                                                                                                    ggTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGaaa                                                                                                                                                  <  2:211504/151‑1 (MQ=255)   ttGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGc                                                                                                                                                >  2:164338/1‑151 (MQ=255)        cAGGTTTTCTAGGATATCTTCCTGAACGCCGTCTTTGGTGCTGTACTCTTGTCGTACTCCATGAATCTCAACCTCGGTCACCGCGCAACCAGGCATCGCTGAGCGCAGAATACGGCGCATTGTCTTACCCAGAGTATGGCGAAAGCCa                                                                                                                                              >  2:323037/1‑148 (MQ=255)         aGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGct                                                                                                                                          >  1:470666/1‑151 (MQ=255)          ggATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGctc                                                                                                                                         <  1:281819/151‑1 (MQ=255)           gATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGctct                                                                                                                                        >  1:330301/1‑151 (MQ=255)           gATTTCCAGGATATCTTCCTGAACGCCTGCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGctct                                                                                                                                        <  2:483750/151‑1 (MQ=255)              ttCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTaa                                                                                                                                      >  1:542282/1‑150 (MQ=255)              ttCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTaa                                                                                                                                      >  1:749114/1‑150 (MQ=255)                 cAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGc                                                                                                                                  >  1:614313/1‑151 (MQ=255)                  aGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCt                                                                                                                                 >  1:133639/1‑151 (MQ=255)                  aGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCt                                                                                                                                 >  2:179866/1‑151 (MQ=255)                   ggATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTc                                                                                                                                <  2:358429/151‑1 (MQ=255)                      tatCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCa                                                                                                                               >  2:955141/1‑149 (MQ=255)                      tatCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCa                                                                                                                               >  1:540454/1‑149 (MQ=255)                      tatCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCa                                                                                                                               >  1:58216/1‑149 (MQ=255)                      tatCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCa                                                                                                                               >  1:80181/1‑149 (MQ=255)                      tatCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCa                                                                                                                               >  2:464984/1‑149 (MQ=255)                       atCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAgg                                                                                                                            >  1:884051/1‑151 (MQ=255)                         cTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGt                                                                                                                          >  1:373949/1‑151 (MQ=255)                           tCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTc                                                                                                                         >  1:124715/1‑150 (MQ=255)                            ccTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCa                                                                                                                        <  2:1059474/150‑1 (MQ=255)                               gAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCtt                                                                                                                    <  1:520384/151‑1 (MQ=255)                                aaCGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTg                                                                                                                   >  2:371122/1‑151 (MQ=255)                                aaCGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTg                                                                                                                   <  2:994525/151‑1 (MQ=255)                                 aCGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTgg                                                                                                                  <  2:357824/151‑1 (MQ=255)                                       tcttTGGTTCTGTACTCATGTAGTACACCATCAGTCTCAACCTCGGTCACCTCGCAACCCGGCATCGATGAGATCAGAATACGGCGCAGTGCGTTACCCAGAGTCTGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGgcgtg                                                                                                              >  2:699993/1‑149 (MQ=255)                                          ttGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTc                                                                                                          <  1:720255/150‑1 (MQ=255)                                            ggTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGaa                                                                                                       <  1:375229/151‑1 (MQ=255)                                               gCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTc                                                                                                    >  1:357420/1‑151 (MQ=255)                                                 tGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCAc                                                                                                  >  2:946235/1‑151 (MQ=255)                                                 tGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCAc                                                                                                  >  2:195558/1‑151 (MQ=255)                                                  gTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACt                                                                                                 >  2:868133/1‑151 (MQ=255)                                                   tACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACtt                                                                                                >  1:907276/1‑151 (MQ=255)                                                    aCTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTg                                                                                               >  1:325968/1‑151 (MQ=255)                                                          gtagtaCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGa                                                                                          <  1:536142/150‑1 (MQ=255)                                                             gtaCACCATCAATCTCAACCTCGGTCCCCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATc                                                                                      <  2:930743/151‑1 (MQ=255)                                                               acacCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCaa                                                                                    <  1:650664/151‑1 (MQ=255)                                                               acacCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCa                                                                                     <  2:878591/150‑1 (MQ=255)                                                                   cATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAg                                                                                <  1:532026/151‑1 (MQ=255)                                                                    aTCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAgg                                                                               <  1:445854/151‑1 (MQ=255)                                                                      cAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGgcg                                                                             >  1:735360/1‑151 (MQ=255)                                                                      cAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGgcg                                                                             <  1:1015352/151‑1 (MQ=255)                                                                       aaTCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGGTGCCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGgcgc                                                                            <  2:831471/151‑1 (MQ=255)                                                                        aTCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGcgcg                                                                           >  1:953069/1‑151 (MQ=255)                                                                            cAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGttt                                                                       <  1:768129/151‑1 (MQ=255)                                                                            cAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGtt                                                                        <  1:228602/150‑1 (MQ=255)                                                                             aaCCTCGGTCAGCGCGCAGCCCGGCGTCGATGAGCGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATAGCAACCAGGCGCgg                                                                          <  2:850342/147‑1 (MQ=255)                                                                             aaCCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGtttt                                                                      <  1:1010090/151‑1 (MQ=255)                                                                                 tCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGa                                                                   >  2:431844/1‑150 (MQ=255)                                                                                  cGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGa                                                                   <  2:872996/149‑1 (MQ=255)                                                                                   ggTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAAc                                                                >  2:730619/1‑151 (MQ=255)                                                                                      cACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAActct                                                             >  1:1052279/1‑151 (MQ=255)                                                                                          gcgcAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTca                                                         <  1:164387/151‑1 (MQ=255)                                                                                           cgcAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTcac                                                        <  2:664265/151‑1 (MQ=255)                                                                                                   ggCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCt                                                >  1:34824/1‑151 (MQ=255)                                                                                                        cGATGAGAGCAGAATACCGCGCAGTGCGTTACCCAGAGTATGCCGAAAGCCAAGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAAATCACTTGCTCGATATCAACCAGGCGCGGTTTTATAAACTCTGTCACACAACCCTGCAtt                                           >  2:949519/1‑151 (MQ=255)                                                                                                           tGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGTGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATtgtg                                        >  1:344979/1‑151 (MQ=255)                                                                                                            gagaGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTgtgt                                       <  1:310865/151‑1 (MQ=255)                                                                                                                 cAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCctct                                  >  2:590613/1‑151 (MQ=255)                                                                                                                 cAGAATACGGCGCAGGGCGTTACCCCGAGTATGTCGAAAGCCACGCTCTAAAGGCTCAAGGGGCACCTTGGCGTGCGGCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGGGTcgt                                    >  2:266262/1‑147 (MQ=255)                                                                                                                    aaTACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCtt                               >  2:765154/1‑151 (MQ=255)                                                                                                                     aTACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCttt                              >  2:972649/1‑151 (MQ=255)                                                                                                                      tACGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTg                             >  1:560587/1‑151 (MQ=255)                                                                                                                        cGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGt                           <  1:398444/151‑1 (MQ=255)                                                                                                                        cGGCGCAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGt                           <  1:685561/151‑1 (MQ=255)                                                                                                                             cAGTGCGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGTGTCAAACTCGCTTGCTCGATATCAACCAGGCGCGGTTTTCGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTag                      <  2:146187/151‑2 (MQ=255)                                                                                                                                  cGTTACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCttt                 >  1:314431/1‑151 (MQ=255)                                                                                                                                     tACCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACt              >  1:637661/1‑151 (MQ=255)                                                                                                                                      aCCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACtt             >  2:876570/1‑151 (MQ=255)                                                                                                                                      aCCCAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACt              <  1:945866/150‑1 (MQ=255)                                                                                                                                        ccAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTgg           <  2:626143/151‑1 (MQ=255)                                                                                                                                        ccAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTgg           <  1:151582/151‑1 (MQ=255)                                                                                                                                        ccAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACGCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTgg           <  2:894888/151‑1 (MQ=255)                                                                                                                                         cAGAGTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGa          <  1:4021/151‑1 (MQ=255)                                                                                                                                          agagTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAg         <  1:179915/151‑1 (MQ=255)                                                                                                                                          agagTATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAg         <  2:617288/151‑1 (MQ=255)                                                                                                                                              tATGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTaa      >  1:822957/1‑150 (MQ=255)                                                                                                                                                tGGCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGc   >  2:477198/1‑151 (MQ=255)                                                                                                                                                 ggCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCt  <  1:700229/151‑1 (MQ=255)                                                                                                                                                 ggCGAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCt  <  1:1009767/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    GGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCT  >  NC_000913/3440777‑3441071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑