Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,443,2770AC39.4% 113.3 / 26.9 72V274G (GTA→GGA) secYpreprotein translocase membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (39/4);  new base C (0/28);  total (39/33)
Fisher's exact test for biased strand distribution p-value = 7.79e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAATACTG  >  NC_000913/3443128‑3443427
                                                                                                                                                       |                                                                                                                                                      
cAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTAc                                                                                                                                                       <  2:190510/151‑1 (MQ=255)
cAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTAc                                                                                                                                                       >  1:376161/1‑151 (MQ=255)
    cGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTAccccc                                                                                                                                                   <  1:502319/151‑1 (MQ=255)
         agagTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCat                                                                                                                                              >  1:1207439/1‑151 (MQ=255)
         agagTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCat                                                                                                                                              >  1:1157646/1‑151 (MQ=255)
             tAACACATAACGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCACCCAGTACCGCCCCCGAACCATGACGCGATGGCCGCCGGGATCAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCAATAtt                                                                                                                                           >  2:1187159/1‑150 (MQ=255)
                   aTAAAGCGGTTGGGCAGGCTGTAAATACAGTGAAATTGTTGTAAGCAAGTTCCAACCAGTACAGAAACCGAAACATGACGCGATGGTCGAAGGGAACAGAATAATACTGGAAGAGAAGATTGCCGGGATTACCCATGCAATATTTAAttt                                                                                                                                     >  2:113718/1‑150 (MQ=255)
                    tAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTc                                                                                                                                    >  1:423456/1‑150 (MQ=255)
                       aGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCg                                                                                                                                >  2:696684/1‑151 (MQ=255)
                       aGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCg                                                                                                                                >  2:570361/1‑151 (MQ=255)
                         cGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCgg                                                                                                                               >  1:140204/1‑150 (MQ=255)
                         cGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGt                                                                                                                              >  1:1199346/1‑151 (MQ=255)
                          ggTTGCCCAGGCTAAAAATACAGCAAAATTGTTGTCACCCATTCCCAACCTGCCCCCCCCCCAACCCATGACGGGATGGTCCCCGGGAACAAAATAATCCTGGAGGCGAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGt                                                                                                                              <  1:883981/150‑1 (MQ=255)
                             tGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAAt                                                                                                                          >  1:1371416/1‑151 (MQ=255)
                               ccggggttaaaaaTCCAGCAAATTTGTTGTCAGCCATTCCCAACCAGTCCCCCCCCCAAACCATGCCGGGAGGGTCGCGGGGAAAAAAATAATACTGGAAGAGAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAAtgt                                                                                                                        <  1:1346068/141‑1 (MQ=255)
                               cccAGGCTGAAAAAACGGCGAAATTTTTGTCGGCCAGTCCCAAACAGTCCCGCCCCCGAACCATGACGCGAGGCTCGCCGGAAACAAAATAATACTGGAAGCGAAGATTGCCGGGATCACCCCCGCCATATTCACTTTCAGCGGTAAAt                                                                                                                          <  2:922509/149‑1 (MQ=255)
                                ccgggctaaaaaTACAGAAAATTTTTTGTCAGCCATTCCAAACCGTCCCCGCCCCCAAACCGTGGCGGTGTGGGCGCGGGGAAAAAAAAAATACTGGAAGCGAAGATGGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAAtgtg                                                                                                                       <  1:1369906/142‑1 (MQ=255)
                                ccAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAAtgtg                                                                                                                       >  1:730886/1‑151 (MQ=255)
                                 cAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAAtgtgt                                                                                                                      >  1:1298232/1‑151 (MQ=255)
                                   ggatGCCAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTATCGCCCCCGAACCATGAGGCGATGGTCCCCGTGAACAGAATAATACTGTAACCGAAGATTGCCGGGATTACCCCCGCTATATTCACTTTCAGCGGTAAAtgt                                                                                                                        >  2:406961/4‑147 (MQ=255)
                                   ggCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGc                                                                                                                    >  2:1289327/1‑151 (MQ=255)
                                    gCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGct                                                                                                                   >  1:863837/1‑151 (MQ=255)
                                      tGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGctct                                                                                                                 >  1:295641/1‑151 (MQ=255)
                                         aaaTACAGAGAATTTTTTGTCGGCGAGTACCAACAAGTCCCGCCCCCGACCAAGGCGGCGAGGGCCGCGGGAAAAAAAATAATACTGGAAGCGAAGATTGCGGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCtg                                                                                                                <  2:1362361/149‑1 (MQ=255)
                                          aaTCCAGCAAAATTTTTGTCACCCAGTTCCAACCAGTCCCCCCCCCGACCCAGGCCGCGATGGTCGCCGGAAAAAAAAAAATCCGGGAAGCGAAGTTTGCCGGAATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgc                                                                                                             <  1:1083367/151‑1 (MQ=255)
                                                gcaaaaTTGTTGTCAGCCAGTCCAACCCAGTCCCGCCCCCGAACCATGACGCGATGGTCGCGGGAAACAAAATAATACTGGAAGCGAAGTTGGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgcag                                                                                                           <  1:784432/144‑1 (MQ=255)
                                                   aattttttgtCAGCCGTTCCCAACCAGTCCCCCCCCCGACCAATGCCGCGATGGTCCCGGGAAACAGAATAATACTGAAAGCAAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgcag                                                                                                           <  1:1180183/138‑1 (MQ=255)
                                                    aaTTGTTGTCAGCCATTCCCAACCGGTCCCCCCCCCGAACCATGCCGGGATGGTCGCGGGGAACAAAATAATACTGGAAGCGAAGTTTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGaca                                                                                                   <  1:615010/151‑1 (MQ=255)
                                                     aTTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGaca                                                                                                   >  2:995421/1‑150 (MQ=255)
                                                         tggtcaccacgTTCCAACAATTCCCTCCCCCACACCGGGACTCGTGGGTCGCCGAGACCAAAATTATACTGGAAGCTAAGATGGCCGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgac                                                                                               <  2:1073059/140‑1 (MQ=255)
                                                          tgtCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgac                                                                                               >  2:1051237/1‑149 (MQ=255)
                                                                ccAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGCCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGc                                                                                       >  2:608524/1‑151 (MQ=255)
                                                                  aGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTg                                                                                     >  1:781107/1‑151 (MQ=255)
                                                                  aGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTg                                                                                     >  1:1362374/1‑151 (MQ=255)
                                                                         aCCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTc                                                                              >  2:217063/1‑151 (MQ=255)
                                                                          ccAGTCCCCCCCCCGAACAAGGCCGGGATGTTCGCGGGGAAAAGAAAAATCCTGGAAGAGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGtt                                                                                <  1:630770/148‑1 (MQ=255)
                                                                                cccccccccAACCCAGGCCGGGATGGCCGCCGGAAAAAGAATAATCCTGGAAGCGAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTcgcg                                                                           <  2:973677/144‑1 (MQ=255)
                                                                                 ccGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGt                                                                       >  2:267245/1‑151 (MQ=255)
                                                                                   gCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGttt                                                                     >  1:1257404/1‑151 (MQ=255)
                                                                                     ccccGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTAc                                                                   >  1:68448/1‑151 (MQ=255)
                                                                                          aacaaggACGCGATGGTCGCCGGAAACAAAATAATACTGGAAGCGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACaa                                                              <  1:102612/145‑1 (MQ=255)
                                                                                          aaCCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACcaca                                                               >  2:797891/1‑150 (MQ=255)
                                                                                             cATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgc                                                           >  2:1184551/1‑151 (MQ=255)
                                                                                                  cgggATGGTCGCCGGGAACAAAATAAACCTGGAAGGGAATATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgcg                                                          <  2:1146850/144‑1 (MQ=255)
                                                                                                   gcgAGGGTCGCCGGAAAAAAAATAATACGGAAAGCGAAGTTTGCGGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgcgg                                                         <  1:821165/147‑1 (MQ=255)
                                                                                                     gATGTTCGCCGGAAAAAAATAAATACTGGAAGCGAAAATTGCGGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGa                                                   <  2:549135/151‑1 (MQ=255)
                                                                                                      aTGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGAc                                                  >  1:301550/1‑151 (MQ=255)
                                                                                                        ggccgcgGGGAAAAAAATAATGCGGGAGGAGAAGATGGCGGGATTCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgcggcc                                                       <  2:1166492/141‑2 (MQ=255)
                                                                                                             cggggAAAAAAATAATACTGGAAGCGAATATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCt                                            <  2:696364/148‑1 (MQ=255)
                                                                                                             cgggaaaCAAAAAAATACGGGAAGCGAAGATTGCCGGGATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCAc                                               <  1:678797/142‑1 (MQ=255)
                                                                                                             ccGGAAAAAAAAAAATACGGGAAGCAAATATCGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTc                                           <  1:888670/151‑1 (MQ=255)
                                                                                                               ggaaaCAAAATAATACTGGAGGCGAAGATTGCGGGATTCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaa                                         <  1:73441/148‑1 (MQ=255)
                                                                                                                 aaaCAAAATAATACTGGAAGCGAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaac                                        <  1:1146149/149‑1 (MQ=255)
                                                                                                                  aaaaaaataatGCTGGAAGCGAATTTTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaa                                         <  1:1051546/143‑1 (MQ=255)
                                                                                                                  aaCAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaaca                                       >  2:642870/1‑150 (MQ=255)
                                                                                                                   aaaaaataatCCTGGAAGCAAATATTGCCGGTATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACaaa                                     <  1:448188/147‑1 (MQ=255)
                                                                                                                   aCAAAATAATACGGGAAGCGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACaaa                                     <  1:1125799/151‑1 (MQ=255)
                                                                                                                       aataatACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATac                                  >  1:95135/1‑150 (MQ=255)
                                                                                                                       aataatACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATac                                  >  2:222149/1‑150 (MQ=255)
                                                                                                                       aaaaacagggaaaGCGAAGTTTCCCGGTATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAAt                                    <  1:1517/137‑1 (MQ=255)
                                                                                                                         taataCTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATacaac                               >  1:1131616/1‑151 (MQ=255)
                                                                                                                            tagtGGAAGCGAGGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATAcaacaa                             <  1:890283/147‑1 (MQ=255)
                                                                                                                            taCTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATAcaacaa                             >  2:457501/1‑150 (MQ=255)
                                                                                                                             aCTGGAAGCAAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACaaa                            <  1:19634/150‑1 (MQ=255)
                                                                                                                                gaaaGCGATGATGGCCGGTTATGCGCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGaa                         <  2:1016540/148‑1 (MQ=255)
                                                                                                                                  aggCGAAGATGCCCGGTATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACg                       <  2:919626/148‑1 (MQ=255)
                                                                                                                                   aGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTc                     >  1:915596/1‑151 (MQ=255)
                                                                                                                                      gAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCATCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACt                  >  1:681654/1‑151 (MQ=255)
                                                                                                                                       aagagtgaagggATAACCCCCGCCATATTCACTTTCAGCGGTACATGTGTGCTCTGTGCAGAATAGACACGACGACCTTGCTGAAGTTCCGAGTAGTTTACCACAATGCGGCGATGACGACGCCCAACAAATACAACAAAGAACGTCACt                  >  2:53875/10‑150 (MQ=255)
                                                                                                                                              ccGGTATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGc                <  1:571971/145‑1 (MQ=255)
                                                                                                                                              ccGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatac          >  2:381679/1‑151 (MQ=255)
                                                                                                                                                   aTTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatac          >  2:264969/1‑146 (MQ=255)
                                                                                                                                                       aCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAATACTg  >  1:434642/1‑151 (MQ=255)
                                                                                                                                                       |                                                                                                                                                      
CAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAATACTG  >  NC_000913/3443128‑3443427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: