Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,352,163	G→A	R158C (CGT→TGT)	sapF ←	antimicrobial peptide ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,352,163	0	G	A	100.0%	268.5 / NA	73	R158C (CGT→TGT)	sapF	antimicrobial peptide ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (43/30);  total (43/30)

AAATATACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAATTTG  >  NC_000913/1352019‑1352310                                                                                                                                                 |                                                                                                                                                    aaaTATACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGtt                                                                                                                                                <  2:222915/150‑1 (MQ=255)  aaTATACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGtttt                                                                                                                                              >  1:784241/1‑151 (MQ=255)    tataCGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATAGCAGCGCGCGCGCCAGACCCAGACACTGttttt                                                                                                                                             <  2:1028724/150‑1 (MQ=255)         gAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACAGATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTGTTGTCCgg                                                                                                                                       <  2:1006028/151‑1 (MQ=255)          aaaTGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCggg                                                                                                                                      >  2:693042/1‑151 (MQ=255)            aTGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCATACACTGTTTTTGTCCGGGTg                                                                                                                                    <  2:811654/151‑1 (MQ=255)              gCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGcc                                                                                                                                  >  1:882354/1‑151 (MQ=255)                ccTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCa                                                                                                                                 >  2:993114/1‑150 (MQ=255)                  tGTTTTTCCTGTAATTCCAGAATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGCCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAAc                                                                                                                               >  2:292728/1‑150 (MQ=255)                    tttttCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACat                                                                                                                             >  2:1021010/1‑150 (MQ=255)                          cTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCg                                                                                                                        >  2:252680/1‑149 (MQ=255)                            gTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGAt                                                                                                                     <  1:802361/150‑1 (MQ=255)                             tAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGAt                                                                                                                     >  1:123082/1‑149 (MQ=255)                                ttCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTa                                                                                                                 >  2:102975/1‑150 (MQ=255)                                ttCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTa                                                                                                                 >  1:750805/1‑150 (MQ=255)                                 tCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAAc                                                                                                               >  2:36982/1‑151 (MQ=255)                                   cAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTg                                                                                                             >  1:535868/1‑151 (MQ=255)                                    aGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTg                                                                                                             >  1:1048828/1‑150 (MQ=255)                                      cATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACg                                                                                                          <  1:561326/151‑1 (MQ=255)                                       aTCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGt                                                                                                         <  1:863774/151‑1 (MQ=255)                                           gATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGTGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATc                                                                                                     >  1:920258/1‑151 (MQ=255)                                            aTTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATcc                                                                                                    <  1:29574/151‑1 (MQ=255)                                             ttAATCAACTGCGAACGCATCGAAATATCCAGTGAAGCGCGGGCTTCATCGGTAATAATGACTTTTGCGCGCAATATCAGCGCGCGCGCCAGACCCAGACAATGTTTTTGTACGGGTGCCAACATATGCGGATAGTAACTGACGTGAt                                                                                                      >  2:769325/1‑148 (MQ=255)                                               aaTCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCgg                                                                                                  <  1:1068977/150‑1 (MQ=255)                                               aaTCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGt                                                                                                 >  2:1024527/1‑151 (MQ=255)                                               aaTCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGt                                                                                                 >  2:364193/1‑151 (MQ=255)                                                   aaCTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTagca                                                                                             <  2:722482/151‑1 (MQ=255)                                                   aaCTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTagc                                                                                              >  1:829555/1‑150 (MQ=255)                                                     cTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTagcagc                                                                                           >  2:1046049/1‑151 (MQ=255)                                                         gAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCaa                                                                                       <  1:903045/151‑1 (MQ=255)                                                         gAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCaa                                                                                       <  2:710748/151‑1 (MQ=255)                                                           aCGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGACAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAAcc                                                                                     <  2:221376/151‑1 (MQ=255)                                                              cATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCAt                                                                                   >  2:964290/1‑150 (MQ=255)                                                               aTCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGAGCCGGTAGCAGCCCAACCATa                                                                                  <  2:914291/150‑1 (MQ=255)                                                                tCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACg                                                                                >  1:822759/1‑151 (MQ=255)                                                                tCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACg                                                                                >  2:531049/1‑151 (MQ=255)                                                                  gACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGc                                                                               >  2:1045418/1‑150 (MQ=255)                                                                   aCATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCAt                                                                             >  1:741581/1‑151 (MQ=255)                                                                    cATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATc                                                                            <  1:754668/151‑1 (MQ=255)                                                                          cAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTc                                                                       >  2:21174/1‑150 (MQ=255)                                                                           aGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCa                                                                      >  1:417677/1‑150 (MQ=255)                                                                               aaGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCaataat                                                                 <  1:963240/151‑1 (MQ=255)                                                                               aaGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCaataat                                                                 >  2:437529/1‑151 (MQ=255)                                                                                    aGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGtt                                                            >  2:1076461/1‑151 (MQ=255)                                                                                              cGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCt                                                   <  2:149747/150‑1 (MQ=255)                                                                                                gCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCACTAATCTGTTTGCGACGCTGtt                                                >  2:231441/1‑151 (MQ=255)                                                                                                 cAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTc                                               <  2:101779/151‑1 (MQ=255)                                                                                                     aatGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCgg                                            <  1:594202/150‑1 (MQ=255)                                                                                                       tGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTc                                         >  1:393915/1‑151 (MQ=255)                                                                                                       tGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTc                                         >  1:471399/1‑151 (MQ=255)                                                                                                          cTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTcc                                        >  2:310690/1‑149 (MQ=255)                                                                                                            tttGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAg                                      <  1:458093/149‑1 (MQ=255)                                                                                                            tttGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGt                                    >  1:172688/1‑151 (MQ=255)                                                                                                             ttGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTc                                   >  2:883075/1‑151 (MQ=255)                                                                                                             ttGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTc                                   >  1:457071/1‑151 (MQ=255)                                                                                                               gggCGCAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCg                                  <  2:882080/150‑1 (MQ=255)                                                                                                                    cAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTc                            >  2:271949/1‑151 (MQ=255)                                                                                                                    cAATATCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTc                            <  1:426812/151‑1 (MQ=255)                                                                                                                        atCAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGgc                        <  1:1038132/151‑1 (MQ=255)                                                                                                                          cAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGgcg                       <  1:973693/150‑1 (MQ=255)                                                                                                                          cAGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGgcg                       >  1:193980/1‑150 (MQ=255)                                                                                                                           aGCGCGCGCGCCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCa                     <  1:383903/151‑1 (MQ=255)                                                                                                                              gcgcgcgcCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTg                  >  2:715303/1‑151 (MQ=255)                                                                                                                              gcgcgcgcCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTg                  >  1:402964/1‑151 (MQ=255)                                                                                                                                   cgcCAGACCCAGACACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGaaa              >  2:189698/1‑150 (MQ=255)                                                                                                                                     ccagacccagacACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATc           >  1:948065/1‑151 (MQ=255)                                                                                                                                      cagacccagacACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATcc          >  2:983275/1‑151 (MQ=255)                                                                                                                                         acccagacACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGa       <  1:668796/151‑1 (MQ=255)                                                                                                                                         acccagacACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGa       <  1:1021338/151‑1 (MQ=255)                                                                                                                                           ccagacACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTACCTGACGTGATCCGGTAGCAGCCCAACCATACGCGTCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTTCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAAt     >  2:23540/1‑151 (MQ=255)                                                                                                                                             agacACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAAttt   <  1:454000/151‑1 (MQ=255)                                                                                                                                             agacACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAAttt   <  1:1107107/151‑1 (MQ=255)                                                                                                                                              gacACTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAATTTg  <  1:693267/151‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                    AAATATACGAAATGCCCTGTTTTTCCTGTAATTCCAGCATCAGATTAATCAACTGCGAACGCATCGACATATCCAGTGAAGCGAGGGCTTCATCGGCAATAATGACTTTTGGGCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAATTTG  >  NC_000913/1352019‑1352310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑