Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,317,438	G→A	A30V (GCG→GTG)	nusA ←	transcription termination/antitermination L factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,317,438	0	G	A	100.0%	302.0 / NA	80	A30V (GCG→GTG)	nusA	transcription termination/antitermination L factor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (38/42);  total (38/42)

CTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCGCGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGG  >  NC_000913/3317292‑3317584                                                                                                                                                   |                                                                                                                                                   cTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCt                                                                                                                                                >  2:1050312/1‑151 (MQ=255)  ttCAAGGGTGATTTCCTTGGTCGGCTGGGTGACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCtt                                                                                                                                               >  1:252471/1‑151 (MQ=255)    cAAGGGTGATTTCCTTGGTCGGCTGGGTGACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCttt                                                                                                                                              <  2:163449/150‑1 (MQ=255)      aGGGTGATTTCCTTGGTCGGCTGGGTGACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTcc                                                                                                                                            >  2:198775/1‑150 (MQ=255)        ggTGATTTCCTTGGTCGGCTGGGTGACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAAt                                                                                                                                         <  2:176595/151‑1 (MQ=255)         gTGATTTCCTTGGTCGGCTGGGTGACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATg                                                                                                                                        <  2:1029571/151‑1 (MQ=255)            aTTTCCTTGGTCGGCTGGGTGACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCtt                                                                                                                                     <  1:1055108/151‑1 (MQ=255)              ttCCTTGGTCGGCTGGGTGACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCg                                                                                                                                   >  1:330928/1‑151 (MQ=255)               tCCTTGGTCGGCTGGGTGACTTCATCAACAACTAACCAGCGACGGATAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCg                                                                                                                                   >  2:119124/1‑150 (MQ=255)                           tGGGTGCCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATTCTTCGAAAATCTtctc                                                                                                                        <  2:200299/149‑1 (MQ=255)                            gggTGACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTcg                                                                                                                       <  2:701881/149‑1 (MQ=255)                             ggTGACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGa                                                                                                                    <  1:355167/151‑1 (MQ=255)                              gTGACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAg                                                                                                                   <  2:380673/151‑1 (MQ=255)                               tGACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAgg                                                                                                                  >  1:19041/1‑151 (MQ=255)                                gACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGt                                                                                                                 >  2:56221/1‑151 (MQ=255)                                        caacaaCTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCtt                                                                                                         >  2:224801/1‑151 (MQ=255)                                        caacaaCTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCt                                                                                                          <  1:417556/150‑1 (MQ=255)                                         aacaacTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCttt                                                                                                        >  2:826664/1‑151 (MQ=255)                                               tAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTg                                                                                                  >  1:822542/1‑151 (MQ=255)                                                aaCCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCAtt                                                                                                   <  1:436515/149‑1 (MQ=255)                                                aaCCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTg                                                                                                  <  1:313425/150‑1 (MQ=255)                                                     gCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACg                                                                                            <  2:650713/151‑1 (MQ=255)                                                       gACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGc                                                                                          >  1:1024407/1‑151 (MQ=255)                                                        aCGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCt                                                                                         <  1:152947/151‑1 (MQ=255)                                                         tggAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCtt                                                                                        >  2:645888/2‑151 (MQ=255)                                                            aaaGTGTCAAAATCACAGCTTTTGCGATCGATCTGTACGTGGACGTCGATCTATTGTTAATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCtt                                                                                        >  2:329672/1‑148 (MQ=255)                                                              agggtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACt                                                                                   >  2:41410/4‑151 (MQ=255)                                                              aGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACt                                                                                   >  2:497361/1‑151 (MQ=255)                                                                tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTAc                                                                                 >  2:799020/1‑151 (MQ=255)                                                                tgccAAAATCACCGCTTTTGCGATCGATCTGTATGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTa                                                                                  >  2:168722/4‑150 (MQ=255)                                                                 gtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTAc                                                                                 >  1:682053/1‑150 (MQ=255)                                                                 gtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACa                                                                                <  1:57955/151‑1 (MQ=255)                                                                   cAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAg                                                                               <  2:59187/150‑1 (MQ=255)                                                                     aaaTCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGcc                                                                             >  1:172477/1‑150 (MQ=255)                                                                         cACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCaaaa                                                                         <  2:984584/150‑1 (MQ=255)                                                                           ccGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAAGGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAAtt                                                                       <  1:14816/150‑1 (MQ=255)                                                                            cGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTc                                                                     <  2:878965/151‑1 (MQ=255)                                                                             gCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCt                                                                    >  2:286455/1‑151 (MQ=255)                                                                                tttGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCtt                                                                   <  1:138070/149‑1 (MQ=255)                                                                                  tGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGtt                                                               >  2:867850/1‑151 (MQ=255)                                                                                           tcTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCggg                                                      >  2:8269/1‑151 (MQ=255)                                                                                              gTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCgg                                                       <  1:168105/147‑1 (MQ=255)                                                                                              gTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCtt                                                   >  1:551528/1‑151 (MQ=255)                                                                                                    ggACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCAcc                                             >  1:422090/1‑151 (MQ=255)                                                                                                     gACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCt                                            >  1:193670/1‑151 (MQ=255)                                                                                                      aCGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCta                                           <  1:350473/151‑2 (MQ=255)                                                                                                       cGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTATCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCt                                            <  2:680821/149‑1 (MQ=255)                                                                                                       cGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATAGCGGGCTTTTCACCTc                                           >  2:488138/1‑150 (MQ=255)                                                                                                              ctctTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGAc                                   >  2:1125574/1‑151 (MQ=255)                                                                                                                ctTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATcc                                       <  2:799662/145‑1 (MQ=255)                                                                                                                ctTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCa                                      <  2:794952/146‑1 (MQ=255)                                                                                                                ctTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTGTTCACCTCATCCAGa                                    <  2:768443/148‑1 (MQ=255)                                                                                                                  tGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACt                                  <  2:119942/148‑1 (MQ=255)                                                                                                                  tGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTa                                 <  2:221681/149‑1 (MQ=255)                                                                                                                  tGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTAt                                <  1:920378/150‑1 (MQ=255)                                                                                                                   gTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTAtt                               <  2:98985/150‑1 (MQ=255)                                                                                                                   gTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTa                              <  2:1084193/151‑1 (MQ=255)                                                                                                                     tCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTaa                             <  2:117971/150‑1 (MQ=255)                                                                                                                      cATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTaaaa                           <  2:252419/151‑1 (MQ=255)                                                                                                                      cATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTaaa                            <  1:650315/150‑1 (MQ=255)                                                                                                                       atatTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAg                          <  1:172297/151‑1 (MQ=255)                                                                                                                        tatTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGt                         >  1:854365/1‑151 (MQ=255)                                                                                                                         atTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGt                         <  2:796987/150‑1 (MQ=255)                                                                                                                           tttttCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTgg                       <  2:347487/150‑1 (MQ=255)                                                                                                                            ttttCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTgggg                     >  2:812672/1‑151 (MQ=255)                                                                                                                             tttCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGACTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTgggg                     >  2:162330/1‑150 (MQ=255)                                                                                                                              ttCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGa                    >  2:373925/1‑150 (MQ=255)                                                                                                                               tCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGaa                   <  2:780803/150‑1 (MQ=255)                                                                                                                                  ttgttgCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAAc                  <  1:27707/148‑1 (MQ=255)                                                                                                                                  ttgttgCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCa                <  2:597778/150‑1 (MQ=255)                                                                                                                                   tgttgCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCACTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGAGGAACCAg               <  2:648429/150‑1 (MQ=255)                                                                                                                                    gttgCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAgg              <  1:447291/150‑1 (MQ=255)                                                                                                                                    gttgCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGt             >  1:48089/1‑151 (MQ=255)                                                                                                                                      tgCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTc           >  1:826901/1‑151 (MQ=255)                                                                                                                                       gCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTc           <  1:1017194/150‑1 (MQ=255)                                                                                                                                         tGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGcc        >  2:835171/1‑151 (MQ=255)                                                                                                                                           tCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCtt      >  1:318807/1‑151 (MQ=255)                                                                                                                                           tCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCtt      >  1:93473/1‑151 (MQ=255)                                                                                                                                              ccAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTg   >  2:454149/1‑151 (MQ=255)                                                                                                                                               cAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTgg  >  1:329020/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                                   CTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCGCGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGG  >  NC_000913/3317292‑3317584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑