Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,413,7350AT51.8% 14.5 / 42.5 27Y2* (TAT→TAA) ydaCDUF1187 family protein, Rac prophage; putative double‑strand break reduction protein
Reads supporting (aligned to +/- strand):  ref base A (2/11);  new base T (14/0);  total (16/11)
Fisher's exact test for biased strand distribution p-value = 5.98e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.03e-05

GTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGT‑GGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATG  >  NC_000913/1413590‑1413884
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gTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTC‑TTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATg                                                                                                                                                  <  2:48575/151‑1 (MQ=255)
     aCTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAac                                                                                                                                               <  2:498445/150‑1 (MQ=255)
         tCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCt                                                                                                                                           <  2:544643/150‑1 (MQ=255)
           tGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTTCATGAacaaccc                                                                                                                                          >  1:506277/1‑145 (MQ=255)
               ctctGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTTCATAAatacacccc                                                                                                                                        >  1:417491/1‑142 (MQ=255)
                   ggAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTTAAAGAAAACCCCCcttattttt                                                                                                                                >  2:309283/1‑151 (MQ=255)
                   ggAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCattattatt                                                                                                                                <  1:327141/151‑1 (MQ=255)
                      aaCGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTATATTAAAACCCCCCTtttttttt                                                                                                                               >  2:313891/1‑149 (MQ=255)
                          ccTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTATATTAACACCTCCCTTATTTTTTTCAGT‑g                                                                                                                        >  2:473456/1‑151 (MQ=255)
                             gCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGT‑gc                                                                                                                       <  1:99001/149‑2 (MQ=255)
                                            gagaGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGT‑GGTTCGTTTATTCCATCt                                                                                                       <  2:448004/150‑1 (MQ=255)
                                                  aTTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTTATTTTTTTAATTAAAACCTCCCTTTTTTTTTTTAGT‑GGTTTGTTTTTTTCATtttt                                                                                                     >  1:189941/1‑146 (MQ=255)
                                                          gCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGAAAATTTTTTTAAAAACACCCCCCTTTTTATTTTCTGT‑GGTTCGGTTTTTTCATCTTTTTAGGGCTTCttt                                                                                        >  2:42162/1‑151 (MQ=255)
                                                                 gATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGT‑GGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCa                                                                                    <  1:383512/148‑1 (MQ=255)
                                                                      cGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTACATTAAAACCTCCCTTATTTTTTTCAGTGGGTTGGTTTTTTCC‑TTTTTTGTGTGGttttttttttctttcccc                                                                            >  2:271241/1‑141 (MQ=255)
                                                                                    ttGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGT‑GGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCgg                                                               <  1:188747/150‑1 (MQ=255)
                                                                                     tGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATATCTTAAAACCCCCATTATTTTTTTCCGT‑GGGTTGTTTTTTTCTTCTTTTTAGTGCTTTTTTTTTTATTTTACCATAACCAGTTccg                                                               >  1:389426/1‑147 (MQ=255)
                                                                                       aaTATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTATATGGAGAACTCCACTTTTTTTTTTTTT‑GGTTTGGTTATTTTATCTTTTTTGTGCTTTTTTTTTTCTTCCACCATAAACCGGTCggg                                                              >  1:324935/1‑148 (MQ=255)
                                                                                        atatCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTAAATTAACACCCCCCCTTTTTTTTTTATT‑GGTTTGTTTTTTTCATATTTTTAGGGCTTTTTTTTTTCTTTCCCCATTACCCGGTCGGGGtt                                                           >  1:126071/1‑150 (MQ=255)
                                                                                            cTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTACATGAACACCTCCCTTATTATTTTCCGT‑GGTTCGGTTTTTTCATCTTTCTAGTGGTTCTTTTTTACTTTCCCCATAAACCGTTCGGGGTTTGCAt                                                      >  2:273656/1‑151 (MQ=255)
                                                                                                       agtagGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTTCATGAAAAACCCCCTTTTTTTTTTCATT‑GGGTTGTTTTTTTTTTCTTTTTAGTGCTTCTTTTTTTCTTTCACCCTAAACGGGTCGGGGTTTGCCTCCGTTTTACtt                                           >  1:103968/1‑151 (MQ=255)
                                                                                                         tagGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAAATTTTTAAATAAAAAACCCCCTTTTTTTTTTCAGT‑GGGTCGTTTTTTTCATCTTTTTAGTGGTTTTTTTTTACTTTCACCATAAACGGGTTGGGGGTCGCATCCGtt                                                 >  2:117812/1‑143 (MQ=255)
                                                                                                                     cttccttTTCAATAGTGGCGGTAATTTTTTACATTAACACCCCCCTTATTTTTTTCAGT‑GGGTCGTTTTTTTCATCTTTTGTGTGCTTTTTTTTTTCTTCCCCCATAAACCGTTCGGGGTTTGCCTCCCTTTCAcctccctccggaa                                 >  2:208178/1‑142 (MQ=255)
                                                                                                                           tttCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGT‑GGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGa                         <  2:423074/149‑1 (MQ=255)
                                                                                                                                       cGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGT‑GGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCg           <  2:82190/151‑1 (MQ=255)
                                                                                                                                           aaTTTTATACATGAACACCTCCATTATTATTTCCAGT‑GGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTAc       >  2:282710/1‑151 (MQ=255)
                                                                                                                                            aTTTTATACATGAACACCTCCATTATTATTTCCAGT‑GGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACt      <  1:177954/151‑1 (MQ=255)
                                                                                                                                                 ataCATGAACACCTCCATTATTATTTCCAGT‑GGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATg  <  2:207782/150‑1 (MQ=255)
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GTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGT‑GGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATG  >  NC_000913/1413590‑1413884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑