Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,604,9370TG43.8% 46.4 / 13.3 33V182G (GTG→GGG) rsmD16S rRNA m(2)G966 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (1/17);  new base G (14/0);  total (16/17)
Fisher's exact test for biased strand distribution p-value = 3.18e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGACGATAAATTTACTGGAAGATAACGGCTGGCTGGCTGACGAAGCCCTGATTTATGTCGAAAGCGAAGTCGAAAACGGTCTGCCCACTGTTCCAGCAAACTGGTCATTACATCGGGAAAAAGTGGCGGGTCA‑GGTGGCTTATCGGCTGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGC‑GTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAC  >  NC_000913/3604802‑3605080
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agaCGATAAATTTACTGGAAGATAACGGCTGGCTGGCTGACGAAGCCCTGATTTATGTCGAAAGCGAAGTCGAAAACGGTCTGCCCACTGTTCCAGCAAACTGGTCATTACATCGGGAAAAAGTGGCGGGTCA‑GGGGGGTTAt                                                                                                                                           >  1:16891/1‑143 (MQ=255)
      tAAATTTACTGGAAGATAACGGCTGGCTGGCTGACGAAGCCCTGATTTATGTCGAAAGCGAAGTCGAAAACGGTCTGCCCACTGTTCCAGCAAACTGGTCATTACATCGGGAAAAAGTGGCGGGGCA‑GGGGGCTTATTGGGTggttta                                                                                                                                >  2:318553/1‑143 (MQ=255)
                       aaCGGCTGGCTGGCTGACGAAGCCCTGATTTATGTCGAAAGCGAAGTCGAAAACGGTCTGCCCACTGTTCCAGCAAACTGGTCATTACATCGGGAAAAAGTGGCGGGTCA‑GGTGGCTTATCGGCTGTATCAACGCGAAGCACAAGGAGaaa                                                                                                            <  2:604415/151‑1 (MQ=255)
                       aaCGGCTGGCTGGCTGACGAAGCCCTGATTTATGTCGAAAGCGAAGTCGAAAACGGTCTGCCCACTGTTCCAGCAAACTGGTCATTACATCGGGAAAAAGTGGCGGGTCA‑GGTGGCTTATCGGCTGTATCAACGCGAAGCACAAGGAGaaa                                                                                                            <  1:240163/151‑1 (MQ=255)
                               gctggctgACGAAGCCCTGATTTATGTCGAAAGCGAAGTCGAAAACGGTCTGCCCACTGTTCCAGCAAACTGGTCATTACATCGGGAAAAAGTGGCGGGGCA‑GGGGGGTTTTTTGGTGTATTAACCCGGAGGACAAAGAGaaa                                                                                                            >  1:514178/1‑143 (MQ=255)
                                  ggctgACGAAGCCCTGATTTATGTCGAAAGCGAAGTCGAAAACGGTCTGCCCACTGTTCCAGCAAACTGGTCATTACATCGGGAAAAAGTGGCGGGGCAGGGGGGCTTTTTGG‑TGTATTAACACCAAACACaaaggggaaaagggg                                                                                                      >  2:419203/1‑133 (MQ=255)
                                          aaGCCCTGATTTATGTCGAAAGCGAAGTCGAAAACGGTCTGCCCACTGTTCCAGCAAACTGGTCATTACATCGGGAAAAAGTGGCGGGTCA‑GGTGGCTTATCGGCTGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTg                                                                                         <  1:296959/151‑1 (MQ=255)
                                             cccTGATTTATGTCGAAAGCGAAGTCGAAAACGGTCTGCCCACTGTTCCAGCAAACTGGTCATTACATCGGGAAAAAGTGGCGGGGCC‑GGGGGGTTATTGGCTGTGTTAACGCGGAGGACAAAGAGGAAGGGATGCTTGTTTATAtt                                                                                          >  1:622740/1‑147 (MQ=255)
                                               cTGATTTATGTCGAAAGCGAAGTCGAAAACGGTCTGCCCACTGTTCCAGCAAACTGGTCATTACATCGGGAAAAAGTGGCGGGGCA‑GGGGGGGTTTCGGCTGTTTTAAAACGGAGGCCAAAGAGGAAGAGATGGTGTTTTATTTTTGTCGt                                                                                    >  1:189725/1‑151 (MQ=255)
                                                     tATGTCGAAAGCGAAGTCGAAAACGGTCTGCCCACTGTTCCAGCAAACTGGTCATTACATCGGGAAAAAGTGGCGGGTCA‑GGTGGCTTATCGGCTGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGtt                                                                               <  2:262991/150‑1 (MQ=255)
                                                       tGTCGAAAGCGAAGTCGAAAACGGTCTGCCCACTGTTCCAGCAAACTGGTCATTACATCGGGAAAAAGTGGCGGGGTA‑GGGGGGGTATAGGCTGTATCAAAGCGAAGGAAAAAGGGAAAAGGATGGTGGTTAATAtttggggttttt                                                                                >  2:246351/1‑137 (MQ=255)
                                                              aGCGAAGTCGAAAACGGTCTGCCCACTGTTCCAGCAAACTGGTCATTACATCGGGAAAAAGTGGCGGGTCA‑GGTGGCTTATCGGCTGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTGGTTTGTTAATGCTCTGc                                                                     <  1:274185/151‑1 (MQ=255)
                                                              aGCGAAGTCGAAAACGGTCTGCCCACTGTTCCAGCAAACTGGTCATTACATCGGGAAAAAGTGGCGGGTCA‑GGGGGGTTATCGGCTGTTTTAAACCGGAACACAAAGGGAAAAGGGTGGTGGTTAATATTTGTTGTTTTTTTAttttct                                                                       >  1:645308/1‑144 (MQ=255)
                                                                 gAAGTCGAAAACGGTCTGCCCACTGTTCCAGCAAACTGGTCATTACATCGGGAAAAAGTGGCGGGTCA‑GGTGGCTTATCGGCTGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGC‑Gtt                                                                 <  1:244093/151‑1 (MQ=255)
                                                                 gAAGTCGAAAACGGTCTGCCCACTGTTCCAGCAAACTGGTCATTACATCGGGAAAAAGTGGCGGGTCA‑GGGGGGTTATTGGCTGTATTAACGCGAAGCACAAAGAGAAAGAGATGGTGGTTTATATTTGTTGTTTTTTTATGCTCTTC‑ggt                                                                 >  1:506819/1‑149 (MQ=255)
                                                                  aaGTCGAAAACGGTCTGCCCACTGTTCCAGCAAACTGGTCATTACATCGGGAAAAAGTGGCGGGGCC‑GGGGGGGTATTGGCTGTATTAAAGCGAAAGAAAAAGGGAAAGGGGTGGTTGGTTATTTTTGTTGTTTGTTAATGttcctcggtt                                                                 >  2:262908/1‑144 (MQ=255)
                                                                       gAAAACGGTCTGCCCACTGTTCCAGCAAACTGGTCATTACATCGGGAAAAAGTGGCGGGTCA‑GGGGGCTTTTCGGCTGTATCAACGCGAAGAACAAGGAGAAAGGGGTGCTGATTAATATTTGTTGGTTGTTTATGCTCTTC‑GTTTtgg                                                             >  1:51387/1‑149 (MQ=255)
                                                                         aaaCGGTCTGCCCACTGTTCCAGCAAACTGGTCATTACATCGGGAAAAAGTGGCGGGTCA‑GGTGGCTTATCGGCTGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGC‑GTTTGGGGAtt                                                         <  2:301402/151‑1 (MQ=255)
                                                                              gTCTGCCCACTGTTCCAGCAAACTGGTCATTACATCGGGAAAAAGTGGCGGGTCA‑GGTGGCTTATCGGCTGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGC‑GTTTGGGGATTTTTaa                                                    <  1:559094/151‑1 (MQ=255)
                                                                                   cccACTGTTCCAGCAAACTGGTCATTACATCGGGAAAAAGTGGCGGGTCC‑GGGGGGGTATTGGCTGTATCAAAACGAAGCACAAAGAGAAAAAGAAGCTGGTTAATATTTGTCGTTTTTTTATTCTCTTCGGTTTGGGGTTTTTTATTctc                                                >  1:373208/1‑149 (MQ=255)
                                                                                    ccACTGTTCCAGCAAACTGGTCATTACATCGGGAAAAAGTGGCGGGTCA‑GGTGGCTTATCGGCTGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGC‑GTTTGGGGATTTTTAATCCTCa                                              <  2:420324/151‑1 (MQ=255)
                                                                                         gTTCCAGCAAACTGGTCATTACATCGGGAAAAAGTGGCGGGTCA‑GGTGGCTTATCGGCTGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGC‑GTTTGGGGATTTTTAATCCTCAAcc                                           <  2:369757/149‑1 (MQ=255)
                                                                                         gTTCCAGCAAACTGGTCATTACATCGGGAAAAAGTGGCGGGTCA‑GGTGGCTTATCGGCTGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGC‑GTTTGGGGATTTTTAATCCTCAACCt                                          <  1:82048/150‑1 (MQ=255)
                                                                                            ccAGCAAACTGGTCATTACATCGGGAAAAAGTGGCGGGTCA‑GGTGGCTTATCGGCTGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGC‑GTTTGGGGATTTTTAATCCTCAACCTGGt                                       <  1:643081/150‑1 (MQ=255)
                                                                                                 aaaCTGGTCATTACATCGGGAAAAAGTGGCGGGTCA‑GGAGGCTTTTCGGCTGTTTTAAAGCGAAGACCAAGGAGAAAAGGATGTTGGATTATTTTTGTCGCTTTTTTATTTTCTTC‑CTTTTGGGGGTTTTTATACTCAAccctcgggattc                                 >  1:685465/1‑141 (MQ=255)
                                                                                                      ggTCATTACATCGGGAAAAAGTGGCGGGTCA‑GGGGGGGTTTCGGCTGTATTAACGCGAAGCACAAGGAGGAAGAGATGCTGGTTTATATTTGTCGTTTTTTTATGCTCTGC‑GGTTTGGGGTTTTTTATTCTCAACCTGGTGGCTCCCCTcc                            >  1:104536/1‑151 (MQ=255)
                                                                                                         cATTACATCGGGAAAAAGTGGCGGGTCA‑GGTGGCTTATCGGCTGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGC‑GTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCAc                         <  1:112124/151‑1 (MQ=255)
                                                                                                          aTTACATCGGGAAAAAGTGGCGGGTCA‑GGTGGCTTATCGGCTGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGC‑GTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACg                        <  1:249331/151‑1 (MQ=255)
                                                                                                                   ggAAAAAGTGGCGGGTCA‑GGTGGCTTATCGGCTGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGC‑GTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGaa               <  1:661491/151‑1 (MQ=255)
                                                                                                                   ggAAAAAGTGGCGGGTCA‑GGTGGCTTATCGGCTGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGC‑GTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGaa               <  1:101970/151‑1 (MQ=255)
                                                                                                                         aGTGGCGGGTCA‑GGTGGCTTATCGGCTGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGC‑GTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCtt         <  1:683092/151‑1 (MQ=255)
                                                                                                                           tGGCGGGTCA‑AGGGGCTTATCCGCTGTATCCACGCGAACATAAAGGAGAAAGGGATTCTGATTAATATTGGTCGGTTGTTAATGCTCTGC‑GGTTGGGGGTTTTTAATCCTCAAACTGGTGCCTCCCTTCCCACGCCCGCTGAATATCtt         >  2:55670/1‑149 (MQ=255)
                                                                                                                                ggTCA‑GGTGGCTTATCGGCTGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGC‑GTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAc  >  1:217955/1‑151 (MQ=255)
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AGACGATAAATTTACTGGAAGATAACGGCTGGCTGGCTGACGAAGCCCTGATTTATGTCGAAAGCGAAGTCGAAAACGGTCTGCCCACTGTTCCAGCAAACTGGTCATTACATCGGGAAAAAGTGGCGGGTCA‑GGTGGCTTATCGGCTGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGC‑GTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAC  >  NC_000913/3604802‑3605080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑