Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,258,974	0	T	G	57.9%	24.9 / 45.6	38	G411G (GGT→GGG)	dinF	oxidative stress resistance protein; putative MATE family efflux pump; UV and mitomycin C inducible protein
Reads supporting (aligned to +/- strand):  ref base T (5/11);  new base G (22/0);  total (27/11)
Fisher's exact test for biased strand distribution p-value = 3.63e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.98e-05

GCTGACCGCTATCTTATCTGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGG  >  NC_000913/4258843‑4259124                                                                                                                                    |                                                                                                                                                       gctgACCGCTATCTTATCTGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGGCGCCCCCGGGTTTGCGCGGGCGCTCCtt                                                                                                                                      >  2:198861/1‑150 (MQ=255)  ctgACCGCTATCTTATCTGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCCCCCCCGGGTTTTCGGTGCCGCTCCtt                                                                                                                                      >  2:510108/1‑149 (MQ=255)   tgACCGCTATCTTATCTGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCCGGGTTTGCGCTGACGCTCCtt                                                                                                                                      >  1:415023/1‑148 (MQ=255)     aCCGCTATCTTATCTGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCCGGGTTTTCGCTGACGCCCCTTACGc                                                                                                                                  >  1:395648/1‑150 (MQ=255)           atcttatctGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGt                                                                                                                            <  1:173335/150‑1 (MQ=255)            tcttatctGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGGCGCCGCAGGGTTTGCGCTGACGCCCCCTACGCTGCCGTgg                                                                                                                          >  1:600135/1‑151 (MQ=255)            tcttatctGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGACGCCGCAGGTTTTGCGCTGTCGCCCCTTACGCTGCCGTgg                                                                                                                          >  1:483953/1‑151 (MQ=255)               tatctGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACCGTATGGCGGTGGCCGCCGCAGGGTTTTCGCTGGCGCGCCTTACGCTGCCGTgg                                                                                                                          >  2:179637/1‑148 (MQ=255)                    ggCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGGCCCCCCAGGGTTTGGGCTTGAGCCCCTTTCGCCCGCGGGGCTggg                                                                                                                     >  2:381080/1‑148 (MQ=255)                    ggCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTaa                                                                                                                  <  1:379905/151‑1 (MQ=255)                                  ccGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGtggc                                                                                                    <  1:100601/151‑1 (MQ=255)                                  ccGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGGTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGGAATCATGCTTTTtgg                                                                                                     >  1:640561/1‑150 (MQ=255)                                   cGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGGTTTTTGCCGACGCTCCTTACGGTGCCCTTGGTGGGGAAACAttgttttggggt                                                                                                   >  2:144179/1‑140 (MQ=255)                                      tggtTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGGCGGCGCCGGGTTTGCGGTGACGCCCCCTACGCCGCCGGGGCTGGGGGATCATGCTTTGtgg                                                                                                     >  2:304062/1‑146 (MQ=255)                                            gCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGGCGCCGCAGGGTTTTCGCTGGCGCTCCTTTCGCTGCCGGGGCCGGGGAAACATGCTTTGggggtgg                                                                                                 >  2:146233/1‑144 (MQ=255)                                               tCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGGCGCCGCCGGGTTTGCGCTGACGCTCCTTACGCTGGCGGGGCTGGGTAATCATGCTTTTTGGGTGGGCTTaaac                                                                                         >  1:624633/1‑147 (MQ=255)                                                       tATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGGCGCCGCAGGTTTTGCGCTGGCGCGCCTTACGCCGCCGTGGCCGGGTAATCATGCTTTGTGGCTGGCCTTAACCGGCttttt                                                                                 >  1:600608/1‑147 (MQ=255)                                                                         tttATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGGTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGGCTGGGATTAACCGTCTTTTTGGGGGTGCGCGGGGTTtct                                                              >  1:490492/1‑150 (MQ=255)                                                                           tataGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGGCGCCGCAGGGTTTGTGCTGTCGCCCCTTACGCTGGCGGGGGTGGGGAATTATGCTTTTTGGGTGGCATTAAACGCCTTTTTTGCGGTGCGCGGGCTTTctct                                                            >  2:568779/1‑150 (MQ=255)                                                                              aGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGGTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGGAATCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGGTGCGCGGGCTTTTTCTggcg                                                        >  1:371557/1‑151 (MQ=255)                                                                              aGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGCGGCCGCCGCGGGGTTTGCGCTGGCGCTCCTTTAGCTGCCCCGGCTTGGGAATTATGCTTTTTGGCTGGGGTTAAGCCTCTTTTTGGCGTTGTCGGGGGTTTTTCTggcg                                                        >  1:49011/1‑151 (MQ=255)                                                                                     aCGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCCGGGTTTGCGCTGACGCTCCCTACGCTGCCGTGGCTGGGGAATCATGCTTTGTGGGTGGCATTAACCGCCTTTTTGGCGTTGCGCGGGCTTTTTTTGGCGGCTATCt                                                 >  1:447924/1‑151 (MQ=255)                                                                                     aCGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCt                                                 <  2:110112/151‑1 (MQ=255)                                                                                      cgcgTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGGTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGGAATCATGCTTTTTGGCTGGCGTTTAACGGCTTTCTTGCGTTGCGCGGGGTTTTTCTggggg                                                       >  2:195419/1‑144 (MQ=255)                                                                                           gCCACCGAAATGCGTAACAGTATGGCGGTGGCCCCCGCCGGGTTTGCGCTGACGCCCCTTTCGCTGCCGTGGGGGGGGAATCATGCTTTGTGGGTGGCCTTAACCGTCTTTCTTGCGTTGCGCGGGGTTTTTCCGGGGGCTTTCTggcgg                                            >  2:103348/1‑150 (MQ=255)                                                                                            ccACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGgcggcg                                          <  2:235197/151‑1 (MQ=255)                                                                                             cACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGGTTTGCGCTGACGCCCCTTACGCTGCCGTGGCTGGGGAATCATGCTTTGTGGCTGGCATTTACCGTCTTTTTGGCGTTGCGCGGGCTTTTTCTGGCGGCTATCTGgcggcg                                          >  1:396049/1‑150 (MQ=255)                                                                                              aCCGAAATGCGTAACAGTATGGCGGTTGCCGCCCCAGCGTTTTCGCTGACGCTCCTTACGCTGCCGCGGGTTGGGAATAATGCTTTGGGGGTGGCATTAACCCTCTTTCTTGCGGTGCGCGGGGGTTTTCTTGCGGCTATCTggcgg                                            >  2:415445/1‑147 (MQ=255)                                                                                              aCCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTc                                        <  2:669140/151‑1 (MQ=255)                                                                                                  aaaTGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTg                                    <  1:411005/151‑1 (MQ=255)                                                                                                   aaTGCGTAACAGTATGGCGGTGGGCGCCCCCGGGTTTTCGCTGACGCTCCTTACGCCGCCGGGGCGGGGGAATCATGCTTTTTGGCTGGGCTTAACCGGCTTTTTGGGGTTGCGCGGGGTTTTTTTGGCGGCCCTCTGGCGGGGGCACTgg                                   >  2:680939/1‑151 (MQ=255)                                                                                                          aaCAGTATGGCGGTGGCCGCCGCCGGTTTTTCGCTGACGCTCCTTACGCTGCCGGGGCTGGGGAATCATGCTTTGTGGCTGGCCTTAACCGCCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGGGTCACTGGCGCAATg                            >  2:527825/1‑151 (MQ=255)                                                                                                           aCAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATgg                           <  1:113318/151‑1 (MQ=255)                                                                                                            cAGTATGGCGGTGGGCGGCCCAGGTTTTGCGCTGACGCCCCCTTCGCCGTCGGGGCCGGGGAATCATGCCTTTTGGCTGGCATTAACCGTCTTTCTGGGGTTGCGCGGGGTTTCTCTGGGGGCCTTCTGGCGGCGGCAATGGAGCAAAgg                           >  2:421715/1‑150 (MQ=255)                                                                                                            cAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGt                          <  1:261190/151‑1 (MQ=255)                                                                                                             aGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGGGGCTGGGGAATCATGCTTTGTGGCTGGCGTTAACCGTCTTTTTGGCGTTGCGCGGGGTTTTTCTGGCGGGTCTCTGGCGGGGTCACTGGGGCAAGGGt                          >  2:230547/1‑150 (MQ=255)                                                                                                                   gCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGt                   <  2:240421/151‑1 (MQ=255)                                                                                                                     ggTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGttt                 <  1:487354/151‑1 (MQ=255)                                                                                                                                    ttttGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACgg  >  1:20340/1‑151 (MQ=255)                                                                                                                                    |                                                                                                                                                       GCTGACCGCTATCTTATCTGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGG  >  NC_000913/4258843‑4259124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑