Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,206,435 | 0 | C | T | 44.1% | 28.7 / 19.4 | 34 | intergenic (‑179/‑28) | ttdR/ttdA | transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit |
Reads supporting (aligned to +/- strand): ref base C (4/15); new base T (15/0); total (19/15) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.24e-06 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCT > NC_000913/3206295‑3206581 | cGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATa < 2:672046/151‑1 (MQ=255) gCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGa < 1:85703/151‑1 (MQ=255) cATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAACAAATAAAAAAAATgg > 1:415326/1‑148 (MQ=255) ttCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGTAG‑tt < 2:540686/150‑1 (MQ=255) ccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCa < 2:155724/151‑1 (MQ=255) ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAGACAAATAaaaaaa > 2:661845/1‑135 (MQ=255) ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGTTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGTCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCAtgatga < 2:299010/149‑1 (MQ=255) ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGc < 2:127040/151‑1 (MQ=255) gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCg < 1:408727/151‑1 (MQ=255) attcTTCTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGCTTCCACACATCTCCAACCACCTTTTTAGTGGTAGTTTGTGGCGGTGTAATTTTTCC‑GTCCAAAACAAAAACTCTAG‑ATGCCCCGATTAGTGCAAGtaata > 1:568488/1‑149 (MQ=255) aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAg < 1:684959/151‑1 (MQ=255) ttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCCGACAAAAAAAAAAAATGGGG‑TTGGCATGATGAGCGgaaagaaaaaacaa > 2:415711/1‑134 (MQ=255) cgcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGG‑AATTTTTTCAGACAAATAAAAAAACAGGGG‑GTGCCATGGAGAGGGAAAGtaataaaaaa > 1:490141/1‑139 (MQ=255) gcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTg < 2:391475/151‑1 (MQ=255) gATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGGAAAAAACAAAAGGCAGGGaa > 1:700436/1‑148 (MQ=255) cACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGG‑TATTTTTTTAGACAAATAAAAAAAATGGAG‑TTGCCATGATGGGCGAAAGGAATAAACAAAAAGGAGGGAATaa > 1:87715/1‑147 (MQ=255) cGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGAAAAAAAAAAAAAAAGGAG‑TTGTCATGATGAGCGGAAAGAATAAAAAAAAAGCAGTGAATaa > 1:449086/1‑145 (MQ=255) aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACag < 2:541531/151‑1 (MQ=255) aCATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑GATTTTTTCCCACAAATACAAAAAAATGAGATTGCC‑TGATGAGCGGGAGTAATAAAGAAAAAGCAGGGAATAAAtttggaaagagt > 1:10472/1‑135 (MQ=255) atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAG‑AATTTTTTCAGAAAAAAAAAAAAACTGGAG‑TTGGCAAGATGAGCGAAAGAAATAAAAAACAAGCCGAGAATAAAATGACagaagatgttg > 2:344970/1‑139 (MQ=255) tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCACACAAAAAAAAAAAATGGAG‑GTGCCATTATGAGCGAAAAAAATAAAAAAAAAGCAGGGAATAAATTGACAGAGATTTTTGCtta > 1:21212/1‑148 (MQ=255) ggCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCa < 2:651812/151‑1 (MQ=255) atatAAAACCACATTTTTAGTGGTAGTTTGGGGCGGTG‑AATTTTTCCAAACAAATACAAAAACGGGAG‑TTGCCTTGATGACCAAAATTAATAAGCAACGGCCAGGGAATAAGTTGACAGAGTTTGTCGCTAACTTTACCGCCATGATTTc < 2:695176/150‑1 (MQ=255) ataaAAACCCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATATAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCt < 2:181727/151‑1 (MQ=255) aTTTTTAGTGGTAGTTTGTGGCGGTG‑GATTTTTTCAGACAAAAAAAAAAACTGGAG‑TTGCCAAGATGAGCGAAAGTAATAAACAAAAGGCAGTGAATAAGTTGACAGAGATTTTCGCTAACTTTACCGCCATGATTTCTACCCGAATGcc > 1:423969/1‑150 (MQ=255) tttttAGTGGTAGTTTGTGGCGGTG‑ACTTTTTTCAGACAAAAACAAAAAATGGGG‑TTGGCCCTATGTGCGCAAGGAAAAAGCAAAAAGCGGTGGAAAAATTGACAGAGATTTTCGCTAACTTTATCGCCCTCATTTCTAcccca > 1:294112/1‑142 (MQ=255) tAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAATAAAAAAAATGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtgatg > 1:517445/1‑150 (MQ=255) gTTTGTGGCGGTG‑AATTTTTTCAGACAAATACAAAAAATGGAG‑GTGCCCTGATGAGCGAAAGTAAAAAGCAAAAAGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTTTACCCGAATGACTGATGACGtggggg > 2:597485/1‑151 (MQ=255) gTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACGGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGtggtgg < 1:671702/151‑1 (MQ=255) ttGTGGCGGTG‑AAATTTTTCAGACAAATAAAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAAAAAAAAACAAGGAGTGAATAAGTTGACAGAGATTTTCGCTAAATTTACCGCCATGATTTTTACCCGAAAGCCTGATGACgtgggg > 2:634495/1‑146 (MQ=255) aaTTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAc > 1:584506/1‑150 (MQ=255) aaTTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCAAAATTAATAAGCAACAGGCATTAAATAATTTCAAAGAGTTTTTCGCTACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACa < 2:691266/151‑1 (MQ=255) tttttCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGc > 2:665870/1‑151 (MQ=255) ttttCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCt > 1:248266/1‑151 (MQ=255) | CGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCT > NC_000913/3206295‑3206581 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |