Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,823,664	T→C	V422A (GTG→GCG)	spoT →	bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,823,664	0	T	C	100.0%	164.1 / NA	43	V422A (GTG→GCG)	spoT	bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/20);  total (23/20)

GAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGTGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAAT  >  NC_000913/3823519‑3823808                                                                                                                                                  |                                                                                                                                                 gAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGCGGgcg                                                                                                                                             >  1:642631/1‑151 (MQ=255)   aTTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGCGGgcgc                                                                                                                                            >  1:52267/1‑150 (MQ=255)         cGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGCGGGCGCACGCGtt                                                                                                                                     >  2:296306/1‑151 (MQ=255)             agCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGCGGGCGCACGCGTTGAcc                                                                                                                                 >  1:238116/1‑151 (MQ=255)                     aTCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGCGGGCGCACGCGTTGACCGCCAGCCt                                                                                                                         >  1:18803/1‑151 (MQ=255)                        cGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGCGGGCGCACGCGTTGACCGCCAGCCTTAc                                                                                                                      >  2:135273/1‑151 (MQ=255)                        cGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGCGGGCGCACGCGTTGACCGCCAGCCTTAc                                                                                                                      >  1:396417/1‑151 (MQ=255)                         gATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGCGGGCGCACGCGTTGACCGCCAGCCTTAcc                                                                                                                     >  1:666107/1‑151 (MQ=255)                              ctTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGCGGGCGCACGCGTTGACCGCCAGCCTTACCCGc                                                                                                                  <  2:464195/149‑1 (MQ=255)                                tCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGCGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTc                                                                                                              >  2:629716/1‑151 (MQ=255)                                    ggATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATCTCGGTCATGCCTGCGCGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGc                                                                                                            >  2:173034/1‑149 (MQ=255)                                       tGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGCGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCg                                                                                                       >  2:9464/1‑151 (MQ=255)                                            tttACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGCGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTAc                                                                                                  >  2:554509/1‑151 (MQ=255)                                               aCGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGCGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAg                                                                                               <  1:295848/151‑1 (MQ=255)                                                 gTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGCGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCg                                                                                             <  1:151885/151‑1 (MQ=255)                                                   tttCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGCGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGt                                                                                           >  1:223796/1‑151 (MQ=255)                                                      cacaCCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGCGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCa                                                                                         <  1:194153/150‑1 (MQ=255)                                                       acacCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGCGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCaaa                                                                                       <  1:655397/151‑1 (MQ=255)                                                             gAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGCGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTg                                                                                 >  2:383016/1‑151 (MQ=255)                                                              aaGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGCGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTg                                                                                 >  1:213218/1‑150 (MQ=255)                                                               aGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGCGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGaa                                                                               <  1:650970/151‑1 (MQ=255)                                                                  gcgcATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGCGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATc                                                                            <  1:178623/151‑1 (MQ=255)                                                                    gcATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGCGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCAt                                                                          >  2:88075/1‑151 (MQ=255)                                                                    gcATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGCGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCAt                                                                          >  1:378793/1‑151 (MQ=255)                                                                              gCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGCGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCg                                                                <  1:103870/151‑1 (MQ=255)                                                                                 gcctgccGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGCGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCggg                                                              >  1:271919/1‑150 (MQ=255)                                                                                  cctgccGGTGCAACGCCCGTCGACGTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGCGGGCGCCCGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGgcg                                                            <  2:563114/151‑1 (MQ=255)                                                                                     gccGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGCGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTc                                                         >  2:120560/1‑151 (MQ=255)                                                                                         gTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGCGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGccc                                                     <  1:582686/151‑1 (MQ=255)                                                                                         gTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGCGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGccc                                                     <  2:498588/151‑1 (MQ=255)                                                                                             aaCGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGCGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAAt                                                 <  1:491263/151‑1 (MQ=255)                                                                                              aCGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGCGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATg                                                <  1:430078/151‑1 (MQ=255)                                                                                                    gTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGCGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCtt                                          <  1:124135/151‑1 (MQ=255)                                                                                                    gTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGCGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCtt                                          <  1:175568/151‑1 (MQ=255)                                                                                                     tCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGCGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTg                                         >  2:644076/1‑151 (MQ=255)                                                                                                           tCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGCGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGa                                    >  2:347563/1‑150 (MQ=255)                                                                                                               ttATGCAGTACATACCGATATCGGTCATGCCTGCGCGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACttt                               >  2:127759/1‑151 (MQ=255)                                                                                                                          aTACCGATATCGGTCATGCCTGCGCGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTc                    >  2:32966/1‑151 (MQ=255)                                                                                                                             ccGATATCGGTCATGCCTGCGCGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGaa                 <  2:372742/151‑1 (MQ=255)                                                                                                                              cGATATCGGTCATGCCTGCGCGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCTAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGaaa                <  2:549580/151‑1 (MQ=255)                                                                                                                                  atCGGTCATGCCTGCGCGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAgcgc            <  1:183338/151‑1 (MQ=255)                                                                                                                                           gCCTGCGCGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCGCGCCaaaa   <  1:682429/151‑1 (MQ=255)                                                                                                                                            ccTGCGCGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAAt  <  1:634829/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                 GAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGTGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAAT  >  NC_000913/3823519‑3823808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑