Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 1,728,926 | T→C | F194L (TTT→CTT) | rnt → | RNase T; exoribonuclease T; structured DNA 3' exonuclease; RNA processing; DNA repair |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,728,926 | 0 | T | C | 98.2% | 218.4 / ‑6.3 | 57 | F194L (TTT→CTT) | rnt | RNase T; exoribonuclease T; structured DNA 3' exonuclease; RNA processing; DNA repair |
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (25/31); total (25/32) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CCACTTTTGACACTGCTGCACTGGCCGGGCTGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCAGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGTTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATCTGCCGCCGAAGAGGTGTAATCGAGTCGATGCCTGATGACATGCAATGATTCAGGCATCTATAGTGAGGCTATTCCACGCATCCTGCATGATA > NC_000913/1728786‑1729067 | ccACTTTTGACACTGCTGCACTGGCCGGGCTGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCAGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGCTTTGTGAAAt < 2:251763/151‑1 (MQ=255) ccACTTTTGACACTGCTGCACTGGCCGGGCTGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCAGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGCTTTGTGAAAt > 1:320432/1‑151 (MQ=255) ttttGACACTGCTGCACTGGCCGGGCTGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCAGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGCTTTGTGAAATCGt > 1:468151/1‑150 (MQ=255) ttGACACTGCTGCACTGGCCGGGCTGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCAGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCa > 1:938575/1‑150 (MQ=255) gACACTGCTGCACTGGCCGGGCTGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCAGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAAcc < 2:11993/151‑1 (MQ=255) gACACTGCTGCACTGGCCGGGCTGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCAGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAAc < 1:209889/150‑1 (MQ=255) acacTGCTGCACTGGCCGGGCTGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCAGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCg < 2:568423/151‑1 (MQ=255) acTGCTGCACTGGCCGGGCTGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCAGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCGCt < 1:454479/151‑1 (MQ=255) acTGCTGCACTGGCCGGGCTGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCAGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCGCt > 1:758192/1‑151 (MQ=255) ctgctgCACTGGCCGGGCTGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCAGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCGCTg > 1:717443/1‑151 (MQ=255) gcACTGGCCGGGCTGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCAGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCGCTGGaa < 2:403477/149‑1 (MQ=255) aCTGGCCGGGCTGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCAGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCGCTGGAAACGt > 2:517022/1‑151 (MQ=255) cTGGCCGGGCTGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCAGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCGCTGGAAACGt < 2:75186/150‑1 (MQ=255) ggCCGGGCTGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCAGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCGCTGGAAACGTCTg > 2:880957/1‑151 (MQ=255) ggCCGGGCTGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCAGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCGCTGGAAACGTCTg > 1:544524/1‑151 (MQ=255) gCCGGGCTGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCAGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCGCTGGAAACGTCTgg < 1:832077/151‑1 (MQ=255) cGGGCTGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCAGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCGCTGGAAACGTCTggg > 1:492483/1‑150 (MQ=255) cTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACAGCTGGCATGGACTTCGACAGCACCCAGGCGCACTCCGCGCTGTACGGCACCGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCGCTGGAAGCGTCTGGGAGGCTGGcc < 2:940493/149‑1 (MQ=255) cGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCAGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAg < 2:754404/140‑1 (MQ=255) cGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCAGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAg > 1:754689/1‑140 (MQ=255) cGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCAGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCt < 1:215836/150‑1 (MQ=255) gACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCAGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATc > 1:364646/1‑151 (MQ=255) aCAAGCCGTATTGTCAAAGGCTGGCCAGACCGCTGGCATGGACTTCGACAGCCCCCAGGCGCACTCCGCGCTTTACGACACCGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATca < 2:420468/151‑2 (MQ=255) aCCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCAGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATCTgccg > 2:347502/1‑151 (MQ=255) cGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCAGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATCTgccgcc > 1:291592/1‑151 (MQ=255) tGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCAGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATCTGCCGCCGAag < 1:441536/150‑1 (MQ=255) cAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCAGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATCTGCCGCCGAAGAgg < 1:649156/150‑1 (MQ=255) aaaGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCAGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATCTGCCGCCGAAGAgg > 2:330480/1‑149 (MQ=255) gCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCAGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGCGCTGCTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATCTGCCGCCGAAGAGGTGTAAt > 2:592778/1‑151 (MQ=255) tGCCAGACCGCTGGCATGGACTTCGACAGCACCCAGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATCTGCCGCCGAAGAGGTGTAATc < 2:931145/149‑1 (MQ=255) cGCTGGCATGGACTTCGACAGCACCCAGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATCTGCCGCCGAAGAGGTGTAATCGAGTCGATGc > 1:574444/1‑151 (MQ=255) gCATGGACTTCGACAGCACCCAGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATCTGCCGCCGAAGAGGTGTAATCGAGTCGATGCCtgat < 1:82338/151‑1 (MQ=255) aTGGACTTCGACAGCACCCAGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATCTGCCGCCGAAGAGGTGTAATCGAGTCGATGCCtgatga < 1:531131/151‑1 (MQ=255) aCTTCGACAGCGCCCAGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGTTTTTTGAAATCGTCAACCGCTGGAAGCGTCTGGGAGGCTGGCCGCTATCTGCCGCCGGAGAGGTGGAATCGAGTCGATGCCTGATGACATg < 2:675526/151‑1 (MQ=255) aCTTCGACAGCACCCAGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATCTGCCGCCGAAGAGGTGTAATCGAGTCGATGCCTGATGACATg < 2:569969/151‑1 (MQ=255) cGACAGCACCCAGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATCTGCCGCCGAAGAGGTGTAATCGAGTCGATGCCTGATGACATGCaa < 2:717163/150‑1 (MQ=255) cGACAGCACCCAGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATCTGCCGCCGAAGAGGTGTAATCGAGTCGATGCCTGATGACATGCaa < 1:750962/150‑1 (MQ=255) cAGCACCCAGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATCTGCCGCCGAAGAGGTGTAATCGAGTCGATGCCTGATGACATGCAATGa < 1:867419/150‑1 (MQ=255) cAGCACCCAGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATCTGCCGCCGAAGAGGTGTAATCGAGTCGATGCCTGATGACATGCAATGAt > 1:548609/1‑151 (MQ=255) gCACCCAGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATCTGCCGCCGAAGAGGTGTAATCGAGTCGATGCCTGATGACATGCAATGATTc > 1:30520/1‑151 (MQ=255) aCCCAGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATCTGCCGCCGAAGAGGTGTAATCGAGTCGATGCCTGATGACATGCAATGATTc > 2:202099/1‑149 (MQ=255) ccAGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATCTGCCGCCGAAGAGGTGTAATCGAGTCGATGCCTGATGACATGCAATGATTCAGGc < 2:667640/151‑1 (MQ=255) aGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATCTGCCGCCGAAGAGGTGTAATCGAGTCGATGCCTGATGACATGCAATGATTCAGGCAt > 1:540030/1‑151 (MQ=255) ggCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATCTGCCGCCGAAGAGGTGTAATCGAGTCGATGCCTGATGACATGCAATGATTCAGGCATc < 1:910944/151‑1 (MQ=255) gcACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATCTGCCGCCGAAGAGGTGTAATCGAGTCGATGCCTGATGACATGCAATGATTCAGGCATCtat > 2:107207/1‑151 (MQ=255) ccGCGCTGTACGACACCGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATCTGCCGCCGAAGAGGTGTAATCGAGTCGATGCCTGATGACATGCAATGATTCAGGCATCTATAGTg < 1:923629/150‑1 (MQ=255) gcTGTACGACACCGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATCTGCCGCCGAAGAGGTGTAATCGAGTCGATGCCTGATGACATGCAATGATTCAGGCATCTATAGTGAGGCt < 2:83570/151‑1 (MQ=255) gTACGACACCGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATCTGCCGCCGAAGAGGTGTAATCGAGTCGATGCCTGATGACATGCAATGATTCAGGCATCTATAGTGAGGCTAtt < 2:291420/151‑1 (MQ=255) acacCGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATCTGCCGCCGAAGAGGTGTAATCGAGTCGATGCCTGATGACATGCAATGATTCAGGCATCTATAGTGAGGCTATTCCAc > 1:753273/1‑150 (MQ=255) acacCGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATCTGCCGCCGAAGAGGTGTAATCGAGTCGATGCCTGATGACATGCAATGATTCAGGCATCTATAGTGAGGCTATTCCACg < 2:467914/151‑1 (MQ=255) acCGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATCTGCCGCCGAAGAGGTGTAATCGAGTCGATGCCTGATGACATGCAATGATTCAGGCATCTATAGTGAGGCTATTCCACGCa < 2:703288/151‑1 (MQ=255) ccGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATCTGCCGCCGAAGAGGTGTAATCGAGTCGATGCCTGATGACATGCAATGATTCAGGCATCTATAGTGAGGCTATTCCACGCa < 2:320237/150‑1 (MQ=255) ccGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATCTGCCGCCGAAGAGGTGTAATCGAGTCGATGCCTGATGACATGCAATGATTCAGGCATCTATAGTGAGGCTATTCCACGCAt > 1:504916/1‑151 (MQ=255) cGAACGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATCTGCCGCCGAAGAGGTGTAATCGAGTCGATGCCTGATGACATGCAATGATTCAGGCATCTATAGTGAGGCTATTCCACGCATc < 1:335971/151‑1 (MQ=255) aCGCACTGCTGTGCTGCTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATCTGCCGCCGAAGAGGTGTAATCGAGTCGATGCCTGATGACATGCAATGATTCAGGCATCTATAGTGAGGCTATTCCACGCATCCTg > 2:191065/1‑151 (MQ=255) ctgctgTGCTGCTTTGTGAAATCGTCACCCGTTGGAAACGTCTGGGAGGCTGGCCGCTATCTGCCGCCGAAGAGGTGTAATCGAGTCGATGCCTGATGACATGCAATGATTCAGGCATCTATAGTGAGGCTATTCCACGCATCCTGCAt < 2:820370/149‑1 (MQ=255) tgctgtgctgCTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATCTGCCGCCGAAGAGGTGTAATCGAGTCGATGCCTGATGACATGCAATGATTCAGGCATCTATAGTGAGGCTATTCCACGCATCCTGCATGat > 2:295915/1‑151 (MQ=255) gctgtgctgcTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATCTGCCGCCGAAGAGGTGTAATCGAGTCGATGCCTGATGACATGCAATGATTCAGGCATCTATAGTGAGGCTATTCCACGCATCCTGCATGata < 2:266521/151‑1 (MQ=255) | CCACTTTTGACACTGCTGCACTGGCCGGGCTGGCACTCGGACAAACCGTATTGTCAAAGGCTTGCCAGACCGCTGGCATGGACTTCGACAGCACCCAGGCGCACTCCGCGCTGTACGACACCGAACGCACTGCTGTGCTGTTTTGTGAAATCGTCAACCGCTGGAAACGTCTGGGAGGCTGGCCGCTATCTGCCGCCGAAGAGGTGTAATCGAGTCGATGCCTGATGACATGCAATGATTCAGGCATCTATAGTGAGGCTATTCCACGCATCCTGCATGATA > NC_000913/1728786‑1729067 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |