Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,724,848	C→A	G300V (GGC→GTC)	kgtP ←	alpha‑ketoglutarate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,724,848	0	C	A	100.0%	261.1 / NA	65	G300V (GGC→GTC)	kgtP	alpha‑ketoglutarate transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (32/33);  total (32/33)

GCAGGGCACACATCACCAGACCAAAAGCGGCATAAGGCGAGGAAACGTTTTGCAATGCTGAGAGAATAGGAACGGTAAAAATGGCTGCCAGCGAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGCCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGAAACAAAGGGAGCCCGCAGCGGTA  >  NC_000913/2724705‑2724994                                                                                                                                                |                                                                                                                                                   gcagGGCACACATCACCAGACCAAAAGCGGCATAAGGCGAGGAAACGTTTTGCAATGCTGAGAGAATAGGAACGGTAAAAATGGCTGCCAGCGAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAg                                                                                                                                             <  2:46811/151‑1 (MQ=255)      gCACACATCACCAGACCAAAAGCGGCATAAGGCGAGGAAACGTTTTGCAATGCTGAGAGAATAGGAACGGTAAAAATGGCTGCCAGCGAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGtt                                                                                                                                        <  1:165113/151‑1 (MQ=255)      gCACACATCACCAGACCAAAAGCGGCATAAGGCGAGGAAACGTTTTGCAATGCTGAGAGAATAGGAACGGTAAAAATGGCTGCCAGCGAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGtt                                                                                                                                        <  1:333149/151‑1 (MQ=255)       cacacaTCACCAGACCAAAAGCGGCATAAGGCGAGGAAACGTTTTGCAATGCTGAGAGAATAGGAACGGTAAAAATGGCTGCCAGCGAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTg                                                                                                                                       <  1:651148/151‑1 (MQ=255)         cacaTCACCAGACCAAAAGCGGCATAAGGCGAGGAAACGTTTTGCAATGCTGAGAGAATAGGAACGGTAAAAATGGCTGCCAGCGAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGaa                                                                                                                                     <  2:262253/151‑1 (MQ=255)          acaTCACCAGACCAAAAGCGGCATAAGGCGAGGAAACGTTTTGCAATGCTGAGAGAATAGGAACGGTAAAAATGGCTGCCAGCGAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAAt                                                                                                                                    >  1:129858/1‑151 (MQ=255)              cACCAGACCAAAAGCGGCATAAGGCGAGGAAACGTTTTGCAATGCTGAGAGAATAGGAACGGTAAAAATGGCTGCCAGCGAACCGAAACATAACATTGAGGTACGGCGACCAATATTATCCGACAGCGCGACAATGAGTGGTTGAATAAg                                                                                                                                 >  1:296792/1‑150 (MQ=255)                ccAGACCAAAAGCGGCATAAGGCGAGGAAACGTTTTGCAATGCTGAGAGAATAGGAACGGTAAAAATGGCTGCCAGCGAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCAt                                                                                                                              >  2:271857/1‑151 (MQ=255)                ccAGACCAAAAGCGGCATAAGGCGAGGAAACGTTTTGCAATGCTGAGAGAATAGGAACGGAAAAAATGGCTGCCAGCGAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCAt                                                                                                                              >  2:382133/1‑151 (MQ=255)                       aaaaGCGGCATAAGGCGAGGAAACGTTTTGCAATGCTGAGAGAATAGGAACGGTAAAAATGGCTGCCAGCGAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATac                                                                                                                        >  1:786736/1‑150 (MQ=255)                          aGCGGCATAAGGCGAGGAAACGTTTTGCAATGCTGAGATAATAGGAACGGTAAAAATGGCTGCCAGCGAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATacaaac                                                                                                                    <  1:298297/151‑1 (MQ=255)                          aGCGGCATAAGGCGAGGAAACGTTTTGCAATGCTGAGAGAATAGGAACGGTAAAAATGGCTGCCAGCGAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATacaaac                                                                                                                    <  1:550067/151‑1 (MQ=255)                           gCGGCATAAGGCGAGGAAACGTTTTGCAATGCTGAGAGAATAGGAACGGTAAAAATGGCTGCCAGCGAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATacaaaca                                                                                                                   <  1:612894/151‑1 (MQ=255)                             ggCATAAGGCGAGGAAACGTTTTGCAATGCTGAGAGAATAGGAACGGTAAAAATGGCTGCCAGCGAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAAt                                                                                                                 <  2:493040/151‑1 (MQ=255)                              gCATAAGGCGAGGAAACGTTTTGCAATGCTGAGAGAATAGGAACGGTAAAAATGGCTGCCAGCGAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATg                                                                                                                >  1:968278/1‑151 (MQ=255)                                aTAAGGCGAGGAAACGTTTTGCAATGCTGAGAGAATAGGAACGGTAAAAATGGCTGCCAGCGAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCg                                                                                                              <  2:993952/151‑1 (MQ=255)                                aTAAGGCGAGGAAACGTTTTGCAATGCTGAGAGAATAGGAACGGTAAAAATGGCTGCCAGCGAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCg                                                                                                              <  1:433692/151‑1 (MQ=255)                                  aaGGCGAGGAAACGTTTTGCAATGCTGAGAGAATAGGAACGGTAAAAATGGCTGCCAGCGAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCgg                                                                                                             <  2:188080/150‑1 (MQ=255)                                  aaGGCGAGGAAACGTTTTGCAATGCTGAGAGAATAGGAACGGTAAAAATGGCTGCCAGCGAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGc                                                                                                            >  1:981618/1‑151 (MQ=255)                                     gCGAGGAAACGTTTTGCAATGCTGAGAGAATAGGAACGGTAAAAATGGCTGCCAGCGAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGt                                                                                                         <  1:288519/151‑1 (MQ=255)                                     gCGAGGAAACGTTTTGCAATGCTGAGAGAATAGGAACGGTAAAAATGGCTGCCAGCGAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGt                                                                                                         <  1:708828/151‑1 (MQ=255)                                        aGGAAACGTTTTGCAATGCTGAGAGAATAGGAACGGTAAAAATGGCTGCCAGCGAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCAt                                                                                                      >  2:610764/1‑151 (MQ=255)                                            aaCGTTTTGCAATGCTGAGAGAATAGGAACGGTAAAAATGGCTGCCAGCGAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATg                                                                                                  <  2:836343/151‑1 (MQ=255)                                               gTTTTGCAATGCTGAGAGAATAGGCACGGTAAAAATGGCTGCCAGCGAACCGAAACATAACATTGAGGGACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATg                                                                                                  >  2:279953/1‑148 (MQ=255)                                                ttttGCAATGCTGAGAGAATAGGAACGGTAAAAATGGCTGCCAGCGAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCAc                                                                                              >  1:61918/1‑151 (MQ=255)                                                         gCTGAGAGAATAGGAACGGTAAAAATGGCTGCCAGCGAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGt                                                                                     >  2:449215/1‑151 (MQ=255)                                                             agagaATAGGAACGGTAAAAATGGCTGCCAGCGAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGgc                                                                                 >  1:215352/1‑151 (MQ=255)                                                                gaATAGGAACGGTAAAAATGGCTGCCAGCGAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGgcatg                                                                              >  2:806875/1‑151 (MQ=255)                                                                 aaTAGGAACGGTAAAAATGGCTGCCAGCGAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGgcatgc                                                                             >  1:547226/1‑151 (MQ=255)                                                                  aTAGGAACGGTAAAAATGGCTGCCAGCGAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGgcatgca                                                                            <  1:997032/151‑1 (MQ=255)                                                                  aTAGGAACGGTAAAAATGGCTGCCAGCGAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGgcatgc                                                                             >  2:434388/1‑150 (MQ=255)                                                                    aGGAACGGTAAAAATGGCTGCCAGCGAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCAtt                                                                          <  1:941456/151‑1 (MQ=255)                                                                        aCGGTAAAAATGGCTGCCAGCGAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCg                                                                      <  2:961852/151‑1 (MQ=255)                                                                        aCGGTAAAAATGGCTGCCAGCGAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCg                                                                      <  1:489744/151‑1 (MQ=255)                                                                          ggTAAAAATGGCTGCCAGCGAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCa                                                                    <  1:50227/151‑1 (MQ=255)                                                                          ggTAAAAATGGCTGCCAGCGAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCa                                                                    <  2:108223/151‑1 (MQ=255)                                                                          ggTAAAAATGGCTGCCAGCGAAACGAAACATAACATTGAGGTACGGCGACAAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGc                                                                     >  2:131956/1‑150 (MQ=255)                                                                             aaaaaTGGCTGCCAGCGAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGt                                                                  >  1:231346/1‑150 (MQ=255)                                                                                       gCCAGCGAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTAcc                                                           >  1:562928/1‑147 (MQ=255)                                                                                       gCCAGCGAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAg                                                         <  1:956051/149‑1 (MQ=255)                                                                                          aGCGAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACt                                                    >  2:682590/1‑151 (MQ=255)                                                                                             gAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCt                                                 >  2:239669/1‑151 (MQ=255)                                                                                              aaCCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTg                                                <  1:615107/151‑1 (MQ=255)                                                                                              aaCCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTg                                                >  1:867488/1‑151 (MQ=255)                                                                                                  gAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGTCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCata                                            <  1:382738/151‑1 (MQ=255)                                                                                                  gAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCata                                            <  1:238070/151‑1 (MQ=255)                                                                                                   aaaCATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCatat                                           >  1:95228/1‑151 (MQ=255)                                                                                                    aacataacatTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCAtata                                          <  2:697699/151‑1 (MQ=255)                                                                                                         aacatTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAagt                                       >  1:373119/1‑149 (MQ=255)                                                                                                          acatTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAagtagt                                    >  2:356933/1‑151 (MQ=255)                                                                                                          acatTGAGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAagtagt                                    >  2:446035/1‑151 (MQ=255)                                                                                                                aGGTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGt                              <  2:89837/151‑1 (MQ=255)                                                                                                                  gTACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGtat                            <  1:868677/151‑1 (MQ=255)                                                                                                                   tACGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGtat                            >  2:930647/1‑150 (MQ=255)                                                                                                                    aCGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGtata                           <  2:652517/150‑1 (MQ=255)                                                                                                                    aCGGCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAg                          <  2:7901/151‑1 (MQ=255)                                                                                                                       gCGACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGaa                        >  2:787265/1‑150 (MQ=255)                                                                                                                         gACCAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGAAACa                     <  2:602399/151‑1 (MQ=255)                                                                                                                           ccAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGAAACaaa                   <  1:731374/151‑1 (MQ=255)                                                                                                                            cAATCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGAAACaa                    >  2:524690/1‑149 (MQ=255)                                                                                                                             aaTCTTATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGAAACAAAgg                 >  1:487623/1‑151 (MQ=255)                                                                                                                                  tATCCGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCACAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGAAACAAAGGGAGcc            >  2:588989/1‑151 (MQ=255)                                                                                                                                      cGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGAAACAAAGGGAGCCCGc         >  2:537091/1‑150 (MQ=255)                                                                                                                                      cGACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGAAACAAAGGGAGCCCGCa        <  1:219266/151‑1 (MQ=255)                                                                                                                                       gACAGCGCGACAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGAAACAAAGGGAGCCCGc         >  2:339244/1‑149 (MQ=255)                                                                                                                                            cgcgaCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGAAACAAAGGGAGCCCGCAGCGGTa  <  1:418872/151‑1 (MQ=255)                                                                                                                                            cgcgaCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGAAACAAAGGGAGCCCGCAGCGGTa  <  1:974600/151‑1 (MQ=255)                                                                                                                                             gcgaCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGAAACAAAGGGAGCCCGCAGCGGTa  >  1:411798/1‑150 (MQ=255)                                                                                                                                             gcgaCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGAAACAAAGGGAGCCCGCAGCGGTa  >  1:873543/1‑150 (MQ=255)                                                                                                                                             gcgaCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGAAACAAAGGGAGCCCGCAGCGGTa  >  2:129833/1‑150 (MQ=255)                                                                                                                                                |                                                                                                                                                   GCAGGGCACACATCACCAGACCAAAAGCGGCATAAGGCGAGGAAACGTTTTGCAATGCTGAGAGAATAGGAACGGTAAAAATGGCTGCCAGCGAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGCCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGAAACAAAGGGAGCCCGCAGCGGTA  >  NC_000913/2724705‑2724994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑