Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,206,435 | 0 | C | T | 52.5% | 31.9 / 85.6 | 61 | intergenic (‑179/‑28) | ttdR/ttdA | transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit |
Reads supporting (aligned to +/- strand): ref base C (2/27); new base T (32/0); total (34/27) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.57e-15 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.42e-05 |
TATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAA > NC_000913/3206290‑3206583 | tATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTCCAGa > 2:689351/1‑150 (MQ=255) aTTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCata > 1:9640/1‑147 (MQ=255) aTTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACa < 2:1020782/151‑1 (MQ=255) cGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATa < 1:361245/151‑1 (MQ=255) gCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAc < 2:141711/151‑1 (MQ=255) cAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAc < 1:833669/150‑1 (MQ=255) cAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTTAGACaaaaa > 1:766245/1‑147 (MQ=255) aCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATaaaaaa > 1:766667/1‑150 (MQ=255) aCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACaaaaa < 2:891088/151‑1 (MQ=255) cATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAAc < 2:345566/151‑1 (MQ=255) ttCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCCGACAAATAAAAAAAAAGGAggt > 1:643115/1‑148 (MQ=255) ccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCa < 2:836118/151‑1 (MQ=255) ctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCatgat < 1:127722/149‑1 (MQ=255) tGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTCAACCAAAAAAAAAAAACGAGTTGGCatgat > 1:8212/1‑148 (MQ=255) gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCg < 1:593968/151‑1 (MQ=255) cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa < 1:57399/151‑1 (MQ=255) gcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAACAAAAAATGGAGTTGCCCTGATGAGCg > 2:470314/1‑149 (MQ=255) cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGaaa < 2:150/151‑1 (MQ=255) cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAATTTTTTTAAACAaaaaaaaaaaattgggtttgcat > 1:651755/1‑127 (MQ=255) aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGAATTTTTCCACACAAATAAAAAAAATGGAGTTGGCCTGTTGGGGGaaa > 2:237319/1‑150 (MQ=255) atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAACTGGAGGTGCCATGATGAGCgga > 2:93673/1‑146 (MQ=255) atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAAAAAAAAAACTGGAGTTGCCATGATGAGCGaa > 2:348243/1‑148 (MQ=255) attcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAACTGGAGTTGGCATGGTGAGCGaaa > 1:852354/1‑147 (MQ=255) ttcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAGCCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACGGGAGGTGCCATGATGAGCGAAAGtaa < 2:829857/150‑1 (MQ=255) tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat < 2:103694/150‑1 (MQ=255) ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAAAAAAAAATGGAGGTGTCATGATGAGCGGAAGgaataa > 2:354146/1‑149 (MQ=255) tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCa < 2:402605/148‑1 (MQ=255) tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCCGACAAAAACAAAAACTGGAGTTGCCATGATGGGGGAAAGTAATAAAAAACacg > 1:946358/1‑148 (MQ=255) ttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTTAATTTTTTCCGACAAAAACAAAAAATGGAGGTGGCATGATTAGCGAAAGTAATAAACaaaaag > 2:318122/1‑145 (MQ=255) ttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAACTGGAGTTGGCCTGATGAGGGAAAGGAAAAAGCaaaa > 2:473892/1‑145 (MQ=255) cgcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAACAAAAACTGGAGTTGCCATGATGAGGGGAAGTAATAAAAAACAAGCa > 1:104004/1‑149 (MQ=255) cgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGAGTTTGCCTGATGAGCGGAAGTAATAAACaaaa > 1:307368/1‑141 (MQ=255) aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGAGGTGGCATGATGAGGGAAAGAAAAAAAAAAAAAGCAGTGAaaaa > 2:545674/1‑150 (MQ=255) aCGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAAAAAAAAATGGGGGTGCCATGATGAGCGGAAGGAATAAACAAAAAGCAGGGAATAAGtt > 2:176065/1‑149 (MQ=255) aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACag < 2:913008/151‑1 (MQ=255) ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGAACAAAACAAAAAATGGAGGTGTCATGATGAGGGGAAGTAATAAGCAACAGGGAGTGAATAAGTTGa > 2:308039/1‑143 (MQ=255) aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAGACAAAAACAAAAAATGGAGGTGGCATGATGAGCGGAAGTAATAAACAACAAGCAGGGAATAAGTTGACaga > 2:470231/1‑145 (MQ=255) aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTg < 2:603481/150‑1 (MQ=255) aTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTc < 2:515812/151‑1 (MQ=255) tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGAAATTTTTTAAAAAAAAAAAAAAAATGGAGGTGCCATGATGAGGGGAAGAAAAAAACAAAAGGGAGAGGATAAAGTGTCAGAGATTGTCGCTaa > 1:475712/1‑150 (MQ=255) atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAACAAAAAAAAAAAATGGAGGTGCCATGATGAGCGGAAATAATAAACAACAGGCAGGGAATAAGTTGAAAGAGATTGTTGCTaa > 1:128415/1‑149 (MQ=255) cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAAAAAAAAATGGAGTTGCCATGATGAGCGGAAGTAATAAGCAAAAGGCAGGGGATAAGATGACAGAGATTGTCGCTAACttt > 2:662653/1‑148 (MQ=255) cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAcc < 2:601236/151‑1 (MQ=255) gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAAAGGGGTTGCCATGATGAGCGAAAGGAATAAAAAAAAAGCAGTGAAAAAGTTGAAAGAGATTGTCGCTAACttt > 2:578220/1‑146 (MQ=255) tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAATAAAAAAACTGGGGTTTCCCTGATGAGCGGAAAAAATAAGCAACAGGCAGTGAATAAATTGACAGAGATTGTCGCTAACTTTACCGcc > 2:871773/1‑151 (MQ=255) aaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAACTGGAGGTGCCCTGATGAGCGGAAGGAAAAAACAAAAAGCAGGGAAAAAGTTGAAAGAGATTGTTGCTAACTTTACCGCCCTGAttt > 2:590548/1‑151 (MQ=255) aaTATAAAACCACATTTTTAGTGGTATTTTGTGGGGGTGAATTTTCCCAGACAAATACAAAAACTGGGGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGAAGTGAATAAGTTGACGGAGATTGTCGCTAACTTTACCGCCATGATTTc < 1:171137/151‑1 (MQ=255) tataAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCAACCAAAAAAAAAACTGGAGTTGTCATTGTGAGCGAAAGGAAAAAACAAAAGGGAAGGGATAAATTGAAAGAGATTTTCGCTCAATTTACCGCCATGGttttt > 1:1056584/1‑148 (MQ=255) ataAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTa < 1:1018585/150‑1 (MQ=255) taAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGGGTTGCCATGATGGGCGGAAGTAATAAACAAAAGGCAGTGAAAAAATTGACAGAGATTGTCGCTAACTTTACCGCCCTGATTTTTAcc > 2:210835/1‑151 (MQ=255) aaaaCCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAAAAAAAAATGGAGTTGTCATGGTGAGCGAAAGTAATAAACAACAAGCAGAGAATAAATTGACAGAGATTGTCGCTAACTTTACCCCCCTGATTTTTAccc > 1:35101/1‑151 (MQ=255) aaaCCCCATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTCCCAGACAAATACAAAAACGGGATTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAGTTTGACAGAGATTGTCACTAATTTTACCGCCATGATTTCTACCCg < 1:533517/151‑1 (MQ=255) ccACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGAGGTGCCATGATGAGGGAAAGAAATAAGCAACAGGCAGGGAATAAGTTGAAAGAGATTGTCGGTAAATTTACCCCCATGATTTTTACCCGaa > 2:283851/1‑150 (MQ=255) cacaTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATg < 2:807656/151‑1 (MQ=255) aTTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCCGCAAAAAACAAAAAATGGAGTTGGCATGGTGGGAGAAAGTAATAAACAAAAAGCAGGGAAAAAAGTGAGAGAAATTGTCGCTCAATTTACCGACAAGCTTTTTATCCGaa > 1:2117/1‑146 (MQ=255) ttttAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAATTAATAAGCAACAGGAAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtg < 2:936831/150‑1 (MQ=255) tGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAACTGGAGGTTCCATGGTGAGCGGAAGTAATAAGCAACAGGCAGAGAATAAGTTGACAGAGATTGTCGCTAACTTTTACGCCATGATTTTTACCCGCATGCCTGATGACg > 1:646656/1‑150 (MQ=255) tttGTGGCGGTGAATTTTTCCAGAAAAATACAAAAACGGGAGTGGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGGGATGGTCGCTAACTTTGCCGCCATGATTTCTACCCGAATGCCTGATGACgtggtg < 1:530857/149‑1 (MQ=255) gtgGCGGTGAATTTTTCCAGACAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGAAATAAGCAACAGGCAGTGAATAAGTTGACAGAGTTTGTCGCTACTTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaa < 2:662388/151‑1 (MQ=255) aaTTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAATTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa < 2:643225/149‑1 (MQ=255) tttCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCt < 2:697740/150‑1 (MQ=255) ccAGACAAATACAAAAACTGGAGTTGCCATGATAAGCAAAAGTAATAAGCAACAGGCAGTAAATAGGTTGACAGAGTTTGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaa < 2:243944/149‑1 (MQ=255) | TATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAA > NC_000913/3206290‑3206583 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |