Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4350CT52.5% 31.9 / 85.6 61intergenic (‑179/‑28)ttdR/ttdAtranscriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (2/27);  new base T (32/0);  total (34/27)
Fisher's exact test for biased strand distribution p-value = 2.57e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.42e-05

TATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAA  >  NC_000913/3206290‑3206583
                                                                                                                                                 |                                                                                                                                                    
tATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTCCAGa                                                                                                                                                  >  2:689351/1‑150 (MQ=255)
 aTTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCata                                                                                                                                                  >  1:9640/1‑147 (MQ=255)
 aTTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACa                                                                                                                                                <  2:1020782/151‑1 (MQ=255)
     cGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATa                                                                                                                                            <  1:361245/151‑1 (MQ=255)
      gCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAc                                                                                                                                           <  2:141711/151‑1 (MQ=255)
       cAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAc                                                                                                                                           <  1:833669/150‑1 (MQ=255)
       cAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTTAGACaaaaa                                                                                                                                            >  1:766245/1‑147 (MQ=255)
           aCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATaaaaaa                                                                                                                                       >  1:766667/1‑150 (MQ=255)
           aCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACaaaaa                                                                                                                                      <  2:891088/151‑1 (MQ=255)
            cATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAAc                                                                                                                                     <  2:345566/151‑1 (MQ=255)
                    ttCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCCGACAAATAAAAAAAAAGGAggt                                                                                                                              >  1:643115/1‑148 (MQ=255)
                       ccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCa                                                                                                                          <  2:836118/151‑1 (MQ=255)
                             ctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCatgat                                                                                                                      <  1:127722/149‑1 (MQ=255)
                              tGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTCAACCAAAAAAAAAAAACGAGTTGGCatgat                                                                                                                      >  1:8212/1‑148 (MQ=255)
                                gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCg                                                                                                                 <  1:593968/151‑1 (MQ=255)
                                 cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                                <  1:57399/151‑1 (MQ=255)
                                  gcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAACAAAAAATGGAGTTGCCCTGATGAGCg                                                                                                                 >  2:470314/1‑149 (MQ=255)
                                   cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGaaa                                                                                                              <  2:150/151‑1 (MQ=255)
                                   cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAATTTTTTTAAACAaaaaaaaaaaattgggtttgcat                                                                                                                         >  1:651755/1‑127 (MQ=255)
                                    aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGAATTTTTCCACACAAATAAAAAAAATGGAGTTGGCCTGTTGGGGGaaa                                                                                                              >  2:237319/1‑150 (MQ=255)
                                     atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAACTGGAGGTGCCATGATGAGCgga                                                                                                               >  2:93673/1‑146 (MQ=255)
                                     atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAAAAAAAAAACTGGAGTTGCCATGATGAGCGaa                                                                                                               >  2:348243/1‑148 (MQ=255)
                                       attcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAACTGGAGTTGGCATGGTGAGCGaaa                                                                                                              >  1:852354/1‑147 (MQ=255)
                                        ttcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAGCCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACGGGAGGTGCCATGATGAGCGAAAGtaa                                                                                                          <  2:829857/150‑1 (MQ=255)
                                         tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat                                                                                                         <  2:103694/150‑1 (MQ=255)
                                            ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAAAAAAAAATGGAGGTGTCATGATGAGCGGAAGgaataa                                                                                                       >  2:354146/1‑149 (MQ=255)
                                                tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCa                                                                                                    <  2:402605/148‑1 (MQ=255)
                                                   tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCCGACAAAAACAAAAACTGGAGTTGCCATGATGGGGGAAAGTAATAAAAAACacg                                                                                               >  1:946358/1‑148 (MQ=255)
                                                    ttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTTAATTTTTTCCGACAAAAACAAAAAATGGAGGTGGCATGATTAGCGAAAGTAATAAACaaaaag                                                                                               >  2:318122/1‑145 (MQ=255)
                                                    ttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAACTGGAGTTGGCCTGATGAGGGAAAGGAAAAAGCaaaa                                                                                                 >  2:473892/1‑145 (MQ=255)
                                                      cgcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAACAAAAACTGGAGTTGCCATGATGAGGGGAAGTAATAAAAAACAAGCa                                                                                             >  1:104004/1‑149 (MQ=255)
                                                        cgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGAGTTTGCCTGATGAGCGGAAGTAATAAACaaaa                                                                                                 >  1:307368/1‑141 (MQ=255)
                                                             aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGAGGTGGCATGATGAGGGAAAGAAAAAAAAAAAAAGCAGTGAaaaa                                                                                     >  2:545674/1‑150 (MQ=255)
                                                                 aCGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAAAAAAAAATGGGGGTGCCATGATGAGCGGAAGGAATAAACAAAAAGCAGGGAATAAGtt                                                                                  >  2:176065/1‑149 (MQ=255)
                                                                    aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACag                                                                             <  2:913008/151‑1 (MQ=255)
                                                                         ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGAACAAAACAAAAAATGGAGGTGTCATGATGAGGGGAAGTAATAAGCAACAGGGAGTGAATAAGTTGa                                                                                >  2:308039/1‑143 (MQ=255)
                                                                           aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAGACAAAAACAAAAAATGGAGGTGGCATGATGAGCGGAAGTAATAAACAACAAGCAGGGAATAAGTTGACaga                                                                            >  2:470231/1‑145 (MQ=255)
                                                                           aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTg                                                                       <  2:603481/150‑1 (MQ=255)
                                                                            aTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTc                                                                     <  2:515812/151‑1 (MQ=255)
                                                                                  tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGAAATTTTTTAAAAAAAAAAAAAAAATGGAGGTGCCATGATGAGGGGAAGAAAAAAACAAAAGGGAGAGGATAAAGTGTCAGAGATTGTCGCTaa                                                                >  1:475712/1‑150 (MQ=255)
                                                                                   atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAACAAAAAAAAAAAATGGAGGTGCCATGATGAGCGGAAATAATAAACAACAGGCAGGGAATAAGTTGAAAGAGATTGTTGCTaa                                                                >  1:128415/1‑149 (MQ=255)
                                                                                        cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAAAAAAAAATGGAGTTGCCATGATGAGCGGAAGTAATAAGCAAAAGGCAGGGGATAAGATGACAGAGATTGTCGCTAACttt                                                            >  2:662653/1‑148 (MQ=255)
                                                                                        cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAcc                                                         <  2:601236/151‑1 (MQ=255)
                                                                                          gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAAAGGGGTTGCCATGATGAGCGAAAGGAATAAAAAAAAAGCAGTGAAAAAGTTGAAAGAGATTGTCGCTAACttt                                                            >  2:578220/1‑146 (MQ=255)
                                                                                           tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAATAAAAAAACTGGGGTTTCCCTGATGAGCGGAAAAAATAAGCAACAGGCAGTGAATAAATTGACAGAGATTGTCGCTAACTTTACCGcc                                                      >  2:871773/1‑151 (MQ=255)
                                                                                                  aaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAACTGGAGGTGCCCTGATGAGCGGAAGGAAAAAACAAAAAGCAGGGAAAAAGTTGAAAGAGATTGTTGCTAACTTTACCGCCCTGAttt                                               >  2:590548/1‑151 (MQ=255)
                                                                                                   aaTATAAAACCACATTTTTAGTGGTATTTTGTGGGGGTGAATTTTCCCAGACAAATACAAAAACTGGGGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGAAGTGAATAAGTTGACGGAGATTGTCGCTAACTTTACCGCCATGATTTc                                              <  1:171137/151‑1 (MQ=255)
                                                                                                     tataAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCAACCAAAAAAAAAACTGGAGTTGTCATTGTGAGCGAAAGGAAAAAACAAAAGGGAAGGGATAAATTGAAAGAGATTTTCGCTCAATTTACCGCCATGGttttt                                             >  1:1056584/1‑148 (MQ=255)
                                                                                                      ataAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTa                                            <  1:1018585/150‑1 (MQ=255)
                                                                                                       taAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGGGTTGCCATGATGGGCGGAAGTAATAAACAAAAGGCAGTGAAAAAATTGACAGAGATTGTCGCTAACTTTACCGCCCTGATTTTTAcc                                          >  2:210835/1‑151 (MQ=255)
                                                                                                        aaaaCCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAAAAAAAAATGGAGTTGTCATGGTGAGCGAAAGTAATAAACAACAAGCAGAGAATAAATTGACAGAGATTGTCGCTAACTTTACCCCCCTGATTTTTAccc                                         >  1:35101/1‑151 (MQ=255)
                                                                                                         aaaCCCCATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTCCCAGACAAATACAAAAACGGGATTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAGTTTGACAGAGATTGTCACTAATTTTACCGCCATGATTTCTACCCg                                        <  1:533517/151‑1 (MQ=255)
                                                                                                            ccACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGAGGTGCCATGATGAGGGAAAGAAATAAGCAACAGGCAGGGAATAAGTTGAAAGAGATTGTCGGTAAATTTACCCCCATGATTTTTACCCGaa                                      >  2:283851/1‑150 (MQ=255)
                                                                                                             cacaTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATg                                    <  2:807656/151‑1 (MQ=255)
                                                                                                                aTTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCCGCAAAAAACAAAAAATGGAGTTGGCATGGTGGGAGAAAGTAATAAACAAAAAGCAGGGAAAAAAGTGAGAGAAATTGTCGCTCAATTTACCGACAAGCTTTTTATCCGaa                                      >  1:2117/1‑146 (MQ=255)
                                                                                                                  ttttAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAATTAATAAGCAACAGGAAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtg                                <  2:936831/150‑1 (MQ=255)
                                                                                                                        tGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAACTGGAGGTTCCATGGTGAGCGGAAGTAATAAGCAACAGGCAGAGAATAAGTTGACAGAGATTGTCGCTAACTTTTACGCCATGATTTTTACCCGCATGCCTGATGACg                          >  1:646656/1‑150 (MQ=255)
                                                                                                                              tttGTGGCGGTGAATTTTTCCAGAAAAATACAAAAACGGGAGTGGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGGGATGGTCGCTAACTTTGCCGCCATGATTTCTACCCGAATGCCTGATGACgtggtg                     <  1:530857/149‑1 (MQ=255)
                                                                                                                                 gtgGCGGTGAATTTTTCCAGACAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGAAATAAGCAACAGGCAGTGAATAAGTTGACAGAGTTTGTCGCTACTTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaa                <  2:662388/151‑1 (MQ=255)
                                                                                                                                          aaTTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAATTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa         <  2:643225/149‑1 (MQ=255)
                                                                                                                                              tttCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCt    <  2:697740/150‑1 (MQ=255)
                                                                                                                                                 ccAGACAAATACAAAAACTGGAGTTGCCATGATAAGCAAAAGTAATAAGCAACAGGCAGTAAATAGGTTGACAGAGTTTGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaa  <  2:243944/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
TATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAA  >  NC_000913/3206290‑3206583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: