Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,487,1630CT41.7% 45.0 / 13.7 37intergenic (+200/‑72)hrpA/ydcFputative ATP‑dependent helicase/DUF218 superfamily protein, SAM‑binding
Reads supporting (aligned to +/- strand):  ref base C (0/21);  new base T (15/0);  total (16/21)
Fisher's exact test for biased strand distribution p-value = 1.80e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

ATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGAACATTACCCCGTTTCCGACGCTTTCGCCGGCAACTATAGATGCCATAAATGTTATCGGACAGTGG  >  NC_000913/1487021‑1487303
                                                                                                                                               |                                                                                                                                              
attaATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCttt                                                                                                                                         <  2:271587/150‑1 (MQ=255)
    aTTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGCTTATGAAATAATATTTTTTCCttt                                                                                                                                         >  2:96825/1‑146 (MQ=255)
       cTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTTAAGAAAAAAAAATTTCTCCTTTccca                                                                                                                                     >  2:126539/1‑144 (MQ=255)
                tAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAACGACAAAAAATTTTCTCCTTTCTCAGTGGTccccct                                                                                                                          >  1:398029/1‑145 (MQ=255)
                 aaGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTtctc                                                                                                                       <  1:349856/151‑1 (MQ=255)
                     gTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACta                                                                                                                   <  2:523007/151‑1 (MQ=255)
                     gTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACta                                                                                                                   <  1:535028/151‑1 (MQ=255)
                       aaaaaTTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACtatt                                                                                                                 <  2:81718/151‑1 (MQ=255)
                        aaaaTTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTTAAGAAAAAACAATTTCTCCTTTTTGAGGGCTCCCCCTCTCCCcatt                                                                                                                 >  1:199198/1‑150 (MQ=255)
                        aaaaTTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACtatta                                                                                                                <  1:345958/151‑1 (MQ=255)
                         aaaTTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTTAAGAAAAAAAAATTTCTCCCTTCATAGTGCCCCCCTTCTCTCtatt                                                                                                                 >  1:643254/1‑149 (MQ=255)
                           aTTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGAAAAAAGAATTTCTCCCTTTTGAGGGGTCCCCCTCTCCCtatttt                                                                                                               >  1:202722/1‑147 (MQ=255)
                              cAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACAAAAAAATTTCTCCTTTTAGAGTGGTCCCCTtcttccttttttct                                                                                                             >  1:397920/1‑137 (MQ=255)
                                       aGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAAGAAAAACAAATTTTTCCTTTTCTAGTGCTTCCCCTCTCACTATTTtcttaacccgcctt                                                                                                  >  2:401590/1‑139 (MQ=255)
                                           aaaTACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGAGGTCCTCCGTGCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTaa                                                                                           <  2:611729/151‑1 (MQ=255)
                                             aTACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGGATTCTTAAgg                                                                                         <  2:254711/151‑1 (MQ=255)
                                              tACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTTAAGAAAAAACAATTTTTTCTTTTCGAGGGGTCCCCTTCTCACCATTTTTTCACGCAGGATtttttaagg                                                                                        >  1:603690/1‑146 (MQ=255)
                                                 aaTTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAAc                                                                                     <  1:139615/151‑1 (MQ=255)
                                                         gctgcAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAtgga                                                                             <  1:553089/151‑1 (MQ=255)
                                                          ctgcAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTTAAGAAAAAAGATTTTCTCCTTTTTGAGTGGTCCCCTTCTCCCTATTTTTTCCCGCCGGATTTTTTAAGGGaagaaaaag                                                                               >  1:705618/1‑142 (MQ=255)
                                                              aaGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTTAAGACAAAAGAATTTCTCCCTTTAAAGGGCCCCGCCTTTCTCCATTATTTCCCCCCCTATTCTTAAAGGGACGATAaagag                                                                            >  1:534390/1‑147 (MQ=255)
                                                              aaGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTTAAGAAAAAAGAATTTCTCCTTTTTGAGGGGTCCGCCTCTCCCTATTTTCTCCCCCCGTATTTTTTAAGGGaagaaaaag                                                                               >  1:682220/1‑138 (MQ=255)
                                                              aaGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTATCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAaggcgg                                                                           <  2:714951/148‑1 (MQ=255)
                                                              aaGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAAGAAAAAAAAATTTCTCCTTTTCGAGGGCTCCCCTTCTCCCTCTTTTTTCACGCCGTATTTTTTAGGGGAAGAAAAAGAGGaa                                                                         >  1:698474/1‑150 (MQ=255)
                                                                 aTGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCAt                                                                     <  2:699276/151‑1 (MQ=255)
                                                                   gAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGa                                                                   <  1:612291/151‑1 (MQ=255)
                                                                   gAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGa                                                                   <  1:412905/151‑1 (MQ=255)
                                                                       aatGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGAACa                                                                <  1:398465/150‑1 (MQ=255)
                                                                              aTCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAAGAAAAACAATTTTCTCCTTTTACAGGGGTCCCCTTTTCCCTATTTTCTCTCCCCGTATTTTTTAAGGAAAGAGAAAGAGGAAACATTAAAATTAacccc                                                        >  1:485366/1‑150 (MQ=255)
                                                                                           ccTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGAACATTACCCCGTTTCCGACGCtt                                            <  2:426924/150‑1 (MQ=255)
                                                                                                 aaGATATGTCAGGCTTGCGGAGTGGCGGTTTAGGAAAAAAAAATTTTTCCTTTTACAGTGGTCCTCCTCTCCCTCTTATTTTAAGCCGTAATTTTTATGGGAAGGGAAGGAGGAAACATAAAAAATACCCCCTTTTCGCCGCctt                                           >  2:676554/1‑145 (MQ=255)
                                                                                                  aGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGAACATTACCCCGTTTCCGACGCTTTCGCCGGc                                    <  1:503260/151‑1 (MQ=255)
                                                                                                    atatGTCAGGCTTGCGGAGTGGCGGTTTATGAAAAAAGATTTTCTCCTTTCCCAGGGGTCCCCCCCTCTCTATTATTTCCCGCGGTATTTTTTAAGGGAGGAGAAAGAGGGAACAAGAAAAATAACCCCTTTCCGCCGCtttttcccg                                     >  1:711162/1‑142 (MQ=255)
                                                                                                             gCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGAACATTACCCCGTTTCCGACGCTTTCGCCGGCAACTATAGATg                         <  2:505334/151‑1 (MQ=255)
                                                                                                                    gAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGAACATTACCCCGTTTCCGACGCTTTCGCCGGCAACTATAGATGCCATaaa                  <  2:514807/151‑1 (MQ=255)
                                                                                                                         gCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGAACATTACCCCGTTTCCGACGCTTTCGCCGGCAACTATAGATGCCATAAATGTTa             <  1:488894/151‑1 (MQ=255)
                                                                                                                                     cATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGAACATTACCCCGTTTCCGACGCTTTCGCCGGCAACTATAGATGCCATAAATGTTATCGGACAGtgg  <  2:462746/151‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                              
ATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGAACATTACCCCGTTTCCGACGCTTTCGCCGGCAACTATAGATGCCATAAATGTTATCGGACAGTGG  >  NC_000913/1487021‑1487303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: