Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,354,284	C→A	G79W (GGG→TGG)	sapC ←	antimicrobial peptide transport ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,354,284	0	C	A	100.0%	175.5 / NA	48	G79W (GGG→TGG)	sapC	antimicrobial peptide transport ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (21/27);  total (21/27)

AGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGAC  >  NC_000913/1354137‑1354428                                                                                                                                                    |                                                                                                                                                 aGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAg                                                                                                                                               <  1:575433/151‑1 (MQ=255)   cGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAgg                                                                                                                                              >  2:264706/1‑150 (MQ=255)     gAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGgaag                                                                                                                                           <  1:449194/151‑1 (MQ=255)     gAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGgaag                                                                                                                                           <  1:633595/151‑1 (MQ=255)       ggCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGgaagaa                                                                                                                                         <  2:83361/151‑1 (MQ=255)         ccGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGaagaaag                                                                                                                                       <  2:533930/151‑1 (MQ=255)          cGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAATCATCGCGTCCGAGGTCGTCAGTCCACAGgaagaa                                                                                                                                         >  2:568749/1‑148 (MQ=255)          cGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGgaagaa                                                                                                                                         >  2:335586/1‑148 (MQ=255)                  gCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGc                                                                                                                              >  1:267693/1‑151 (MQ=255)                    cccGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGcc                                                                                                                             >  2:328742/1‑150 (MQ=255)                           aaGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGcgcg                                                                                                                     >  2:551028/1‑151 (MQ=255)                           aaGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGcgcg                                                                                                                     <  2:51948/151‑1 (MQ=255)                               tGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGAcc                                                                                                                  >  1:539193/1‑150 (MQ=255)                                gCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCAt                                                                                                                >  1:132734/1‑151 (MQ=255)                                  ccAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCa                                                                                                                 <  2:573099/148‑1 (MQ=255)                                    aGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGAcg                                                                                                            <  1:73176/151‑1 (MQ=255)                                       aCCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGAcggcg                                                                                                         <  2:441067/151‑1 (MQ=255)                                        ccAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGAcggcg                                                                                                         <  2:707456/150‑1 (MQ=255)                                         cAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACggcggc                                                                                                       <  2:62625/151‑1 (MQ=255)                                             ccACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCaat                                                                                                    >  2:4447/1‑150 (MQ=255)                                                     cgtcgCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATaa                                                                                           <  2:281980/151‑1 (MQ=255)                                                            gCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCgaga                                                                                     >  1:537288/1‑150 (MQ=255)                                                                gCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTg                                                                                <  2:227856/151‑1 (MQ=255)                                                                  gTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACGTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCt                                                                              <  2:454977/151‑1 (MQ=255)                                                                   tCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTg                                                                             >  1:705419/1‑151 (MQ=255)                                                                    caccacAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTg                                                                             <  2:534938/150‑1 (MQ=255)                                                                       cacaAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCg                                                                         >  1:632117/1‑151 (MQ=255)                                                                         caAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGAt                                                                       <  1:639113/151‑1 (MQ=255)                                                                           aaTGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGc                                                                     >  1:127120/1‑151 (MQ=255)                                                                                  cacccacGGTTGGCGCAGCTCCGATCAGTAAACGGCTTAAAACATCGAGTCCGAGGACGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTAATAACCGAGAAATTGCTGGTCGATGCCGTAAg                                                               >  2:591821/1‑150 (MQ=255)                                                                                         ggTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAAc                                                       <  1:383287/151‑1 (MQ=255)                                                                                           ttGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAAc                                                       >  2:200860/1‑149 (MQ=255)                                                                                               cgcAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGCAAGGCGCAAACCAgccg                                                 >  2:69427/1‑151 (MQ=255)                                                                                                      ccGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCaaaa                                           <  2:580710/150‑1 (MQ=255)                                                                                                        gCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAAt                                         >  2:516108/1‑150 (MQ=255)                                                                                                          tCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATac                                       >  2:670737/1‑150 (MQ=255)                                                                                                          tCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGACaaaaa                                          >  2:327618/1‑147 (MQ=255)                                                                                                            aGTAAACGGCCTAAAACATCGCGTCCGAGGGCGTCCGTCCACAGGAAGACAGAAACTTCGCCATAGCGCGACCATGGCGGCGGCAATAATTGATAACCGAGAAAATGCTGGTCGAATCCGTAAGGCCCAAAACAGCCGCCAAAAAt                                         >  2:302946/1‑146 (MQ=255)                                                                                                              tAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGt                                  >  1:16352/1‑151 (MQ=255)                                                                                                                aaCGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTAc                                <  2:62479/151‑1 (MQ=255)                                                                                                                          aaCATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGcccc                      <  1:349868/151‑1 (MQ=255)                                                                                                                            cATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCcg                     <  2:361489/150‑1 (MQ=255)                                                                                                                               cgcgTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCgcgc                  <  1:216014/150‑1 (MQ=255)                                                                                                                               cgcgTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCgcgc                  <  2:343535/150‑1 (MQ=255)                                                                                                                                     cGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCg             <  2:354300/149‑1 (MQ=255)                                                                                                                                           cgtcAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGc      <  2:182948/150‑1 (MQ=255)                                                                                                                                            gtcAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCg    <  1:387322/151‑1 (MQ=255)                                                                                                                                              cAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGAc  <  1:498326/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                 AGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGAC  >  NC_000913/1354137‑1354428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑