Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,751,5370AC40.2% 126.0 / 20.7 82V111G (GTT→GGT) yiaTputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base A (35/14);  new base C (0/33);  total (35/47)
Fisher's exact test for biased strand distribution p-value = 7.07e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAACGCCCAGCGCAGGTGTCAGCGACAGACGACCTATCTGCATTTTGTGGAATACCGATAGCTCCCCCACCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCATTTTTTTCACTTTGCGACAGAATAAAACCTAACGTGGTC  >  NC_000913/3751388‑3751686
                                                                                                                                                         |                                                                                                                                                     
gaACGCCCAGCGCAGGTGTCAGCGACAGACGACCTATCTGCATTTTGTGGAATACCGATAGCTCCCCCACCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAAc                                                                                                                                                      >  1:509464/1‑151 (MQ=255)
 aaCGCCCAGCGCAGGTGTCAGCGACAGACGACCTATCTGCATTTTGTGGAATACCGATAGCTCCCCCACCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAAcc                                                                                                                                                     >  2:1002822/1‑151 (MQ=255)
 aaCGCCCAGCGCAGGTGTCAGCGACAGACGACCTATCTGCATTTTGTGGAATACCGATAGCTCCCCCACCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAAcc                                                                                                                                                     >  1:1261116/1‑151 (MQ=255)
  aCGCCCAGCGCAGGTGTCAGCGACAGACGACCTATCTGCATTTTGTGGAATACCGATAGCTCCCCCACCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAAccc                                                                                                                                                    <  1:916050/151‑1 (MQ=255)
     cccAGCGCAGGTGTCAGCGACAGACGACCTATCTGCATTTTGTGGAATACCGATAGCTCCCCCACCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAAccccc                                                                                                                                                  >  2:1098961/1‑150 (MQ=255)
     cccAGCGCAGGTGTCAGCGACAGACGACCTATCTGCATTTTGTGGAATACCGATAGCTCCCCCACCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCg                                                                                                                                                 <  2:1099830/151‑1 (MQ=255)
               gtgtCAGCGACAGACGACCTATCTGCATTTTGTGGAATACCGATAGCTCCCCCACCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGt                                                                                                                                       >  2:398282/1‑151 (MQ=255)
                  tCAGCGACAGACGACCTATCTGCATTTTGTGGAATACCGATAGCTCCCCCACCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACt                                                                                                                                    >  1:1245778/1‑151 (MQ=255)
                   cAGCGACAGACGACCTATCTGCATTTTGTGGAATACCGATAGCTCCCCCACCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACt                                                                                                                                    >  1:448768/1‑150 (MQ=255)
                    aGCGACAGACGACCTATCTGCATTTTGTGGAATACCGATAGCTCCCCCACCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTAt                                                                                                                                  >  1:959696/1‑151 (MQ=255)
                    aGCGACAGACGACCTATCTGCATTTTGTGGAATACCGATAGCTCCCCCACCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTAt                                                                                                                                  >  1:710224/1‑151 (MQ=255)
                     gCGACAGCCGCCCTATCTGCTTTTTGTGAAATACCGATACCCCCCCCCCCCAGCCTTTGCTGTTATCCAGAACGCCCGCAGCTGCAGAGGTTTTCCCGTTTCCCCACCGCTGGTGGTAATCCCAGGCACCCCCCGCCATAGCCGTACTAt                                                                                                                                  <  2:559396/150‑1 (MQ=255)
                      cGACAGACGACCTATCTGCATTTTGTGGAATACCGATAGCTCCCCCACCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATc                                                                                                                                 >  1:1023431/1‑150 (MQ=255)
                       gACAGACGACCTATCTGCATTTTGTGGAATACCGATAGCTCCCCCACCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCAc                                                                                                                               >  1:873050/1‑151 (MQ=255)
                        aCAGACGACCTATCTGCATTTTGTGGAATCCCGATAGCTCCCCCCCCCAGCCGTTGCTGTTATCCAGACCGTCCGCAGCTGCAGAGGCTTTCACGTTTCCCCAACGCTCGTGGTGATACCAGGCACCCCCCGCCATAGCCGTACTATCAc                                                                                                                               <  1:914600/150‑1 (MQ=255)
                          aGACGACCTATCTGCATTTTGTGGAATACCGATAGCTCCCCCACCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCt                                                                                                                            >  2:871656/1‑151 (MQ=255)
                          aGACGACCTATCTGCATTTTGTGGAATACCGATAGCTCCCCCACCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCt                                                                                                                            >  2:584225/1‑151 (MQ=255)
                                ccTATCTGCATTTTGTGGAATACCGATAGCTCCCCCACCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCa                                                                                                                       >  2:90553/1‑150 (MQ=255)
                                ccTATCTGCATTTTGTGGAATACCGATAGCTCCCCCACCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCa                                                                                                                       >  1:921200/1‑150 (MQ=255)
                                catATCTCCATTTGGTGAAATACCGATAGCCCCCCCCCCCGCCCGTTGCTTTTACCCAGAACGCCCGCAGCTGCAGAGCCTTCCACGTTCCCCCAACGCCGGGGTTGACACAAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATc                                                                                                                        <  2:962266/147‑1 (MQ=255)
                                 cTATCTGATTTTGGTGGAATCCCGATAGCCCCCCCCCCCAGCCTTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGCCTTTCACTGTCCCCCACCCCTCGGGTTGATACCGGGCAACCCCCGCCATAGCCGTACTATCa                                                                                                                                <  2:892743/140‑1 (MQ=255)
                                    tcggctttttGTGGAATCCCGATAGCCCCCCCCCCCACCCTTTGCTGTTATCCAGAACGTCCGCACCGGCAGAGGTTTTCCCGTTTCCCCACCGCTCGTGGTCATCCCGGCCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCt                                                                                                                   <  1:1065382/144‑1 (MQ=255)
                                       gCATTTTGTGAAATCCCGATAGCCCCCCCCCCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGAGGAGGCTTTCCCGGTCCCCCACCGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCt                                                                                                                   <  1:414235/147‑1 (MQ=255)
                                          ttttGTGGAATACCGATAGCTCCCCCACCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCAt                                                                                                            >  1:944409/1‑151 (MQ=255)
                                           tttGTGGAATGCCGATACCCCCCCCCCCCAGCCGTTGCTGTAACCCAAATCCCCAGCAGTCGCGAAGTTTCCCCCGGTCCCCCACCGCCCGTGGGAACCCCGGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCAt                                                                                                            <  2:722118/150‑1 (MQ=255)
                                           tttGTGGAATACCGATAGCCCCCCCCCCCACCCGTTGCTTTTACCCAGAACGCCCGCAGCTGCAGAGGCTTTCCCGTTCCCCCAACCCTCGTGGTAATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTg                                                                                                               <  2:477042/147‑1 (MQ=255)
                                            ttGTGGAATACGGATATCTCCCCCACCCAGCCTTGGCTGTTTTCCAGACCGTCCCCAGCTGCAGATGCTTCCCCGTTCCCCAAACCCTGGTGGTATTCCTAGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGc                                                                                                              <  2:1084594/147‑1 (MQ=255)
                                                   aTACCGATAGCTCCCCCACCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATTGCATAATc                                                                                                   >  2:638747/1‑151 (MQ=255)
                                                    tACCGATAGCTCCCCCACCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCg                                                                                                  >  2:824227/1‑151 (MQ=255)
                                                      ccGATAGCCCCCCCCCCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCCCGGTCCCCCACCGCTCGGGGTGATACCAGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTc                                                                                                <  1:682666/151‑1 (MQ=255)
                                                        gATAGCCCCCCCCCCCAGCCGTTGCTTTTACCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCCCGTGGGGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCAt                                                                                              <  1:1258555/151‑1 (MQ=255)
                                                          tacccccccccccCAGCCGTTGTTGTTATCCAGAACGCCCGCAGCTGCAGAGGTTTTCACGTTCCCCCACCGCTCGTGGTGATCCCAGCCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCAt                                                                                              <  1:629184/144‑1 (MQ=255)
                                                               cccccACCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGt                                                                                       >  2:781722/1‑151 (MQ=255)
                                                                ccccccCAAGCCGTGGCTGTTATCAAAACGGCCCGCAGCGGGAGAGCCTTCCACGGTCCCCCAACGCTCGGGGTAATCCCGGCCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTa                                                                                      <  2:954032/151‑1 (MQ=255)
                                                                  cgccccaGACGTTGGCTTTGATCCAGACCCGCCGCGGCTGAGAGGGCTTCATGGTTCCCCCAACTCGCGTGGTGGTACCAGGAACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAg                                                                                     <  2:852093/148‑1 (MQ=255)
                                                                   cacccaGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAggg                                                                                   >  1:389983/1‑151 (MQ=255)
                                                                     cccaGCCTTTGCTTTTACCCAAACCGCCCGCAGCGGCAGAGTCTTTCACGGTTCCCCAACGCTCGGGGTGATACCAGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAggg                                                                                   <  2:880438/149‑1 (MQ=255)
                                                                       caccctttGCTGTTATCCAGAACGTCCGCAGCTGCAGAGCCTTTCCCGTTTCCCCAACGCTCGTGGTAATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCaa                                                                               <  2:411740/145‑1 (MQ=255)
                                                                       caGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCa                                                                                >  2:82929/1‑150 (MQ=255)
                                                                             ttGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTcc                                                                          >  2:1864/1‑150 (MQ=255)
                                                                             ttGCTGTTATCCAGAACGCCCGCAGCTGAAGAGTCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGAACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTcc                                                                          <  1:412115/150‑1 (MQ=255)
                                                                                gCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGc                                                                       >  2:1150956/1‑151 (MQ=255)
                                                                                      aTCCAGAGCGGCCGCAGCTCGGGAGCCTTTCACGGTTCCCCATCGCTCGTGTGGATCCCAGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCg                                                                      <  2:412528/146‑1 (MQ=255)
                                                                                           gAACGCCCGCAGCTGCAGAGGCTTTCCCGGTTCCCCAACGCTCGTGGTGATCCCAGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAgg                                                             <  1:133253/150‑1 (MQ=255)
                                                                                                 ccGCAGCTGCGGAGGCTTTCCCGTTCCCCCGACGCCCTGGGTAAAACCAGACAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGt                                                      <  1:689221/151‑1 (MQ=255)
                                                                                                 ccGCAGCTGCAGAGGTTTTCCCGTTTCCCCAACGTTCGGGGTGTTCCCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATg                                                          <  1:858390/147‑1 (MQ=255)
                                                                                                 ccGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGt                                                      >  1:1108314/1‑151 (MQ=255)
                                                                                                 ccGCAGCGCCAGGGCTTTTCCCGGTCCCCAACCGCTCGTGGTCAACCCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATg                                                          <  2:69716/147‑1 (MQ=255)
                                                                                                   gCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTc                                                     >  1:163786/1‑150 (MQ=255)
                                                                                                   gCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTc                                                     >  2:387063/1‑150 (MQ=255)
                                                                                                      gCTGCAGAGGCTTTCACGGTTCCCCACCGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAgg                                                  <  1:584242/150‑1 (MQ=255)
                                                                                                        tGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCt                                                >  2:623491/1‑150 (MQ=255)
                                                                                                         gCAAAGTCTTGCAGGGTCCCCCAACGCCCGTGGCAACACAAGCAAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGt                                                      <  1:1142218/143‑1 (MQ=255)
                                                                                                             gagCTTTCCACGTTTCCCCAACGCGCGGGGTGATACCAGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAg                                             <  2:285111/147‑1 (MQ=255)
                                                                                                                 tttCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCAttt                                      >  2:910610/1‑151 (MQ=255)
                                                                                                                   tccacttttCCCCAACGGTCGTGTTGTACCCGGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCAt                                        <  1:6913/141‑1 (MQ=255)
                                                                                                                   tCACGGTTCCCCAACGCTGGGGGTGATACCGGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCAtttt                                     <  2:1222746/150‑1 (MQ=255)
                                                                                                                     ccgggTTCCCCAACGCCGGGGGGGATCCCGGGAACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCAtt                                       <  1:71832/144‑1 (MQ=255)
                                                                                                                     cACGGTTCCCCACCGCTCGTGGTGATACCAGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCAttttt                                    <  1:678315/150‑1 (MQ=255)
                                                                                                                     cACGGTCCCCCAACGCTCGGGTTGACCCCGGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGTATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCAtttttt                                   <  2:944362/151‑1 (MQ=255)
                                                                                                                       cGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCATTTTTTTc                                 >  2:925064/1‑151 (MQ=255)
                                                                                                                        ggTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCATTTTTTTc                                 >  1:210505/1‑150 (MQ=255)
                                                                                                                        ggTCCCCCAACGCTCGGGGTGATCCCAGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCAttttttt                                  <  2:853582/149‑1 (MQ=255)
                                                                                                                         gTTTCCCAACGCCTCGTGTTAACACCGGGCACCCACCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCAttttttt                                  <  2:630997/149‑1 (MQ=255)
                                                                                                                          ttcccccACCCCTGGTGGTATTACCAGCCACCCCCCGCCATAGCCGTACTATCACGCTTATCAATCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCAttttttt                                  <  2:957912/145‑1 (MQ=255)
                                                                                                                           tCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCATTTTTTTCACtt                             >  1:39922/1‑151 (MQ=255)
                                                                                                                             ccccAGCGCTCGTGGTAATCCCAGGCACCCCCCGCCATAGCCGTACTACCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCATTTTTTTCACttt                            <  1:1153339/151‑1 (MQ=255)
                                                                                                                             ccccAGCGCTCGTGGTAATACCAGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCATTTTTTTCACtt                             <  2:1261050/150‑1 (MQ=255)
                                                                                                                             ccccAACGCTCGTGGTGATCCCAGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCATTTTTTTCACttt                            <  1:245869/151‑1 (MQ=255)
                                                                                                                             ccccAACGCCGGGGGTGATACCTGCCCCCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCATTTTTTTCa                                <  2:818098/147‑1 (MQ=255)
                                                                                                                                      ctggtggtgATACCCGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCATTTTTTTCACTTTGCGACa                      <  2:988896/146‑1 (MQ=255)
                                                                                                                                      cTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCATTTTTTTCACTTTGCGAc                       >  2:173866/1‑148 (MQ=255)
                                                                                                                                         gtggtgACACCAGCCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCATTTTTTTCACTTTGCGACAGAATa                 <  2:1241946/151‑1 (MQ=255)
                                                                                                                                         gtggtaATACCAGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCATTTTTTTCACTTTGCGACAg                     <  1:470300/147‑1 (MQ=255)
                                                                                                                                         gtggtaATACCAGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCATTTTTTTCACTTTGCGACAGa                    <  1:999936/148‑1 (MQ=255)
                                                                                                                                          ttgtgATACCAGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCATTTTTTTCACTTTGCGACAGAATa                 <  2:321099/148‑1 (MQ=255)
                                                                                                                                          tggggATACCAGCCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCATTTTTTTCACTTTGCGACAGAATa                 <  2:91466/150‑1 (MQ=255)
                                                                                                                                           ggtgATCCCAGCCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCATTTTTTTCACTTTGCGACAGaa                   <  2:921154/147‑1 (MQ=255)
                                                                                                                                              gATACCAGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCATTTTTTTCACTTTGCGACAGAATAAAAc             <  1:1128792/150‑1 (MQ=255)
                                                                                                                                              gATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCATTTTTTTCACTTTGCGACAGAATAAAAcc            >  1:650953/1‑151 (MQ=255)
                                                                                                                                                   cAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCATTTTTTTCACTTTGCGACAGAATAAAACCTAACg       >  1:1016568/1‑151 (MQ=255)
                                                                                                                                                    aGGCAACCCCCGCCATAGCCGTACTATCACAATTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGAAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCATTTTTTTCACTTTGCGACAGAATAAAACCTAACg       >  2:1219143/1‑150 (MQ=255)
                                                                                                                                                        aaCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCATTTTTTTCACTTTGCGACAGAATAAAACCTAACGTGGTc  >  1:336555/1‑151 (MQ=255)
                                                                                                                                                         |                                                                                                                                                     
GAACGCCCAGCGCAGGTGTCAGCGACAGACGACCTATCTGCATTTTGTGGAATACCGATAGCTCCCCCACCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCATTTTTTTCACTTTGCGACAGAATAAAACCTAACGTGGTC  >  NC_000913/3751388‑3751686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: