Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,181,786 G→T G181V (GGT→GTT)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,181,7860GT98.8% 307.3 / ‑4.3 82G181V (GGT→GTT) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (47/34);  total (48/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

GACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTT  >  NC_000913/4181638‑4181933
                                                                                                                                                    |                                                                                                                                                   
gacaACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGttt                                                                                                                                                   >  2:1257272/1‑151 (MQ=255)
   aaCGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCct                                                                                                                                                <  1:937473/151‑1 (MQ=255)
   aaCGGAACCTTTGTTATCAGCGCTACAGAGCGAGTTTTCGCTTCACAGCTGCACCCTACTCCAGCCGACTTTTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGAAAAGTGCTGTATAACGCGCGTATCATCCCTTTCTGTGTTTCct                                                                                                                                                >  2:328152/1‑151 (MQ=255)
      ggTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCctggc                                                                                                                                             <  1:467047/151‑1 (MQ=255)
        tACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCctggctg                                                                                                                                           >  1:1016524/1‑151 (MQ=255)
           cTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGAc                                                                                                                                        >  1:1114318/1‑151 (MQ=255)
               gTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCg                                                                                                                                    >  1:784898/1‑151 (MQ=255)
               gTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCg                                                                                                                                    <  2:678188/151‑1 (MQ=255)
               gTTATCAACGGTACTGAGCGTGTGATCGTTTCCCAGCTGCACCGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCg                                                                                                                                    <  2:478326/151‑1 (MQ=255)
                     aaCGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCg                                                                                                                              >  2:489455/1‑151 (MQ=255)
                     aaCGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCg                                                                                                                              <  1:1067512/151‑1 (MQ=255)
                        ggTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATc                                                                                                                           <  2:1169138/151‑1 (MQ=255)
                         gTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATcc                                                                                                                          >  2:244882/1‑151 (MQ=255)
                           aCTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCg                                                                                                                         >  1:849886/1‑150 (MQ=255)
                             tGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGa                                                                                                                        >  1:431018/1‑149 (MQ=255)
                             tGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGa                                                                                                                        >  2:267303/1‑149 (MQ=255)
                              gAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAgg                                                                                                                     <  2:242429/151‑1 (MQ=255)
                              gAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAgg                                                                                                                     <  2:1121249/151‑1 (MQ=255)
                              gAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAg                                                                                                                      <  1:512488/150‑1 (MQ=255)
                                   tgtTATCGTTTCCCAGCTGCACCGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACaa                                                                                                                 >  2:850777/1‑150 (MQ=255)
                                    gtTATCGTTTCCCAGCTGCACCGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAAcc                                                                                                               >  1:727683/1‑151 (MQ=255)
                                     ttATCGTTTCCCAGCTGCACCGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAAcc                                                                                                               >  2:1150372/1‑150 (MQ=255)
                                       aTCGTTTCCCAGCTGCACCGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTg                                                                                                             <  2:1247751/150‑1 (MQ=255)
                                        tCGTTTCCCAGCTGCACCGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGtt                                                                                                           >  2:1113640/1‑151 (MQ=255)
                                          gTTTCCCAGCTGCACCGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTcg                                                                                                         <  1:1110059/151‑1 (MQ=255)
                                          gTTTCCCAGCTGCACCGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTcg                                                                                                         <  2:101032/151‑1 (MQ=255)
                                             tCCCAGCTGCACCGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTcgta                                                                                                       <  2:729552/150‑1 (MQ=255)
                                              cccAGCTGCACCGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTcgtacg                                                                                                     >  2:993517/1‑151 (MQ=255)
                                                 aGCTGCACCGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTAt                                                                                                  <  2:289872/151‑1 (MQ=255)
                                                 aGCTGCACCGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTAt                                                                                                  >  1:1249815/1‑151 (MQ=255)
                                                     gCACCGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGa                                                                                               <  2:448864/150‑1 (MQ=255)
                                                      cACCGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGAcc                                                                                             >  2:866931/1‑151 (MQ=255)
                                                            aGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGcc                                                                                       >  2:1180479/1‑151 (MQ=255)
                                                               ccGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTa                                                                                    <  2:465123/151‑1 (MQ=255)
                                                                  ggCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGCAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAAc                                                                                 >  2:586412/1‑151 (MQ=255)
                                                                     gTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAActgc                                                                              >  1:984021/1‑151 (MQ=255)
                                                                       cttcttTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAActgcct                                                                            >  2:997731/1‑151 (MQ=255)
                                                                         tcttTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTGCGTATCGACCGTCGCCGTAAActgcct                                                                            <  1:567565/149‑1 (MQ=255)
                                                                            ttGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCg                                                                         <  1:893958/149‑1 (MQ=255)
                                                                            ttGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGa                                                                        <  2:806595/150‑1 (MQ=255)
                                                                             tGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGa                                                                        >  2:350739/1‑149 (MQ=255)
                                                                             tGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGAcc                                                                      >  1:98211/1‑151 (MQ=255)
                                                                             tGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGAcc                                                                      >  2:798938/1‑151 (MQ=255)
                                                                             tGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGAc                                                                       <  1:811274/150‑1 (MQ=255)
                                                                              gACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGAcc                                                                      >  1:572421/1‑150 (MQ=255)
                                                                               aCTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACcat                                                                    >  2:66742/1‑151 (MQ=255)
                                                                                  ccGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACcatcat                                                                 <  2:405641/151‑1 (MQ=255)
                                                                                  ccGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACcatcat                                                                 >  2:283653/1‑151 (MQ=255)
                                                                                   cGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCAtt                                                                >  1:957506/1‑151 (MQ=255)
                                                                                   cGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCAtt                                                                <  1:1132582/151‑1 (MQ=255)
                                                                                      cAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTg                                                             >  2:503388/1‑151 (MQ=255)
                                                                                         aGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTgcgc                                                          <  1:79640/151‑1 (MQ=255)
                                                                                           gTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTgcgcgc                                                        >  2:1091442/1‑151 (MQ=255)
                                                                                                aCCCACGCTTCGGGTAAAGTGCTGTATAACCCGCGTCTCCTCCCTTGCCGTGTTTGCTGGCTGGACTTCTAAGTCGATCTGAAGGACAACCTGTTTGTACGTATCGACCGTCGTCGTAACCTGCCTGCGACCATCATTCTGCGCGCCCTg                                                    <  2:401526/150‑1 (MQ=255)
                                                                                                 cccACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGa                                                   >  2:446693/1‑150 (MQ=255)
                                                                                                  ccACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAAc                                                 >  1:1258493/1‑151 (MQ=255)
                                                                                                    aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACt                                                >  1:530592/1‑150 (MQ=255)
                                                                                                     ctctTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTac                                              >  1:78392/1‑151 (MQ=255)
                                                                                                       ctTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTacac                                            >  2:473880/1‑151 (MQ=255)
                                                                                                        ttCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTAcacc                                           >  1:1189535/1‑151 (MQ=255)
                                                                                                           gggTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTAcaccac                                         >  2:852950/1‑150 (MQ=255)
                                                                                                           gggTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTAcaccac                                         >  2:138244/1‑150 (MQ=255)
                                                                                                           gggTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTAcaccac                                         >  2:1266030/1‑150 (MQ=255)
                                                                                                            ggTAAAGTGCTGTATAACGCGCGTATCATTCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACcaca                                        <  1:1256296/150‑1 (MQ=255)
                                                                                                               aaaGTGCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACagag                                     <  2:643703/150‑1 (MQ=255)
                                                                                                                    gCTGTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATc                               <  2:456459/151‑1 (MQ=255)
                                                                                                                       gTATAACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTc                            >  2:298529/1‑151 (MQ=255)
                                                                                                                        tataACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACGCCACAGAGCAGATCCTc                            <  2:512903/150‑1 (MQ=255)
                                                                                                                        tataACGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCg                           >  1:100394/1‑151 (MQ=255)
                                                                                                                            aCGCGCGTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCt                       >  1:212310/1‑151 (MQ=255)
                                                                                                                              gcgcgTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGt                     <  1:188761/151‑1 (MQ=255)
                                                                                                                                  gTATCATCCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCt                  <  2:211961/150‑1 (MQ=255)
                                                                                                                                    atcatcCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTg               >  2:1061770/1‑151 (MQ=255)
                                                                                                                                      catcCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGa              >  2:473732/1‑150 (MQ=255)
                                                                                                                                        tcCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGaaa            >  2:1015259/1‑150 (MQ=255)
                                                                                                                                        tcCCTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCAACATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGaaa            >  2:596145/1‑150 (MQ=255)
                                                                                                                                          ccTTACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGaaaa           >  2:541180/1‑149 (MQ=255)
                                                                                                                                             tACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGtt       <  1:989947/150‑1 (MQ=255)
                                                                                                                                             tACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGtt       >  1:1083051/1‑150 (MQ=255)
                                                                                                                                             tACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGtt       >  2:843130/1‑150 (MQ=255)
                                                                                                                                             tACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGtt       <  1:904265/150‑1 (MQ=255)
                                                                                                                                             tACCGTGTTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGt        <  1:34777/149‑1 (MQ=255)
                                                                                                                                              aCCGTGTTTCCTGGCTGAACTTCGAATTCAATCCGAACGACAACCTCTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCCACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAATTa      <  2:28134/150‑1 (MQ=255)
                                                                                                                                                 gtgtTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCtt  <  1:600549/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTT  >  NC_000913/4181638‑4181933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: