Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,257,602	A→T	N163I (AAT→ATT)	lexA →	transcriptional repressor of SOS regulon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,257,602	0	A	T	100.0%	247.1 / NA	66	N163I (AAT→ATT)	lexA	transcriptional repressor of SOS regulon
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (35/31);  total (35/31)

CGCGTCAGCGGGATGTCGATGAAAGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCC  >  NC_000913/4257454‑4257746                                                                                                                                                     |                                                                                                                                                 cgcgTCAGCGGGATGTCGATGAAAGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTa                                                                                                                                                <  2:1062019/151‑1 (MQ=255)    gTCAGCGGGATGTCGATGAAAGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTaa                                                                                                                                               >  1:429471/1‑149 (MQ=255)      cAGCGGGATGTCGATGAAAGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTc                                                                                                                                           <  2:577706/151‑1 (MQ=255)       aGCGGGATGTCGATGAAAGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCg                                                                                                                                          >  2:218082/1‑151 (MQ=255)          gggATGTCGATGAAAGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGaa                                                                                                                                        >  2:1087269/1‑150 (MQ=255)          gggATGTCGATGAAAGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAAc                                                                                                                                       >  2:1070388/1‑151 (MQ=255)           ggATGTCGATGAAAGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACt                                                                                                                                      <  1:1028325/151‑1 (MQ=255)           ggATGTCGATGAAAGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACt                                                                                                                                      >  2:1208068/1‑151 (MQ=255)            gATGTCGATGAAAGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTg                                                                                                                                     >  1:829736/1‑151 (MQ=255)               gTCGATGAAAGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTg                                                                                                                                  >  1:623823/1‑151 (MQ=255)                 cGATGAAAGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGcc                                                                                                                                >  2:1034168/1‑151 (MQ=255)                      aaaGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGaaa                                                                                                                           <  2:426364/151‑1 (MQ=255)                       aaGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGaaa                                                                                                                           >  1:682703/1‑150 (MQ=255)                        aGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAAt                                                                                                                         <  1:60467/151‑1 (MQ=255)                          atatCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAg                                                                                                                       <  2:142149/151‑1 (MQ=255)                           tatCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGc                                                                                                                      >  1:29515/1‑151 (MQ=255)                           tatCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGc                                                                                                                      >  1:495397/1‑151 (MQ=255)                            atCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGc                                                                                                                      <  2:37707/150‑1 (MQ=255)                              cGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGa                                                                                                                    <  1:257369/150‑1 (MQ=255)                               ggCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGt                                                                                                                  <  1:1070257/151‑1 (MQ=255)                                 cATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGttt                                                                                                                >  1:11101/1‑151 (MQ=255)                                 cATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGttt                                                                                                                <  1:293454/151‑1 (MQ=255)                                 cATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGttt                                                                                                                <  2:640183/151‑1 (MQ=255)                                 cATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGtt                                                                                                                 <  2:165324/150‑1 (MQ=255)                                   ttATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTa                                                                                                               >  1:1234176/1‑150 (MQ=255)                                        gatggTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCa                                                                                                          >  2:750418/1‑150 (MQ=255)                                               aCTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGt                                                                                                  >  1:21501/1‑151 (MQ=255)                                               aCTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGt                                                                                                  >  2:1167041/1‑151 (MQ=255)                                                  tGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTg                                                                                                <  2:651670/150‑1 (MQ=255)                                                                 aaaCTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCagag                                                                                <  1:178838/151‑1 (MQ=255)                                                                      cAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTc                                                                            >  1:642603/1‑150 (MQ=255)                                                                       cggATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCAc                                                                          >  2:118498/2‑151 (MQ=255)                                                                            gTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCAtt                                                                      <  2:1241310/150‑1 (MQ=255)                                                                            gTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTg                                                                     >  2:984576/1‑151 (MQ=255)                                                                            gTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTg                                                                     <  1:484136/151‑1 (MQ=255)                                                                                gTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAgg                                                                 <  1:395107/151‑1 (MQ=255)                                                                                gTAACGGTCAGGACGTTGTCGCACGAATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAgg                                                                 >  2:612667/1‑151 (MQ=255)                                                                                    cGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCt                                                              <  1:929825/150‑1 (MQ=255)                                                                                      gTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGc                                                           <  1:81233/151‑1 (MQ=255)                                                                                      gTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGc                                                           >  2:728006/1‑151 (MQ=255)                                                                                       tCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGc                                                           >  2:475363/1‑150 (MQ=255)                                                                                           gTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTg                                                      <  2:1029851/151‑1 (MQ=255)                                                                                                tGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGt                                                  <  2:770449/150‑1 (MQ=255)                                                                                                  tCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGtt                                                 >  1:132163/1‑149 (MQ=255)                                                                                                      aCGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGGTAtt                                              >  2:426955/1‑148 (MQ=255)                                                                                                          aTTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACg                                       <  1:1249589/151‑1 (MQ=255)                                                                                                            tgatgaCGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACgg                                      <  2:408699/150‑1 (MQ=255)                                                                                                             gatgaCGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCg                                    >  1:660942/1‑151 (MQ=255)                                                                                                                gaCGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGGTATTTGCAAccggga                                   >  2:900647/1‑144 (MQ=255)                                                                                                                 aCGAAGTTATCGTTAAGCGCCTGAAAAATCAGGGCATTAAAGTCGAACTGTGGCCGGAAAATAGCGAGTTTGAACCAATTGTCGTTGACTTTCGTCAGCAGAGCTTGACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGGGACt                                 <  2:955520/150‑1 (MQ=255)                                                                                                                  cGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGGTGGGGGTTTTGGCAACGGCggcg                                 >  2:1039564/1‑146 (MQ=255)                                                                                                                   gAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTTGGGTTATTTGCAACGGCGACt                                 >  2:559104/1‑148 (MQ=255)                                                                                                                      gTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGt                           <  2:829706/151‑1 (MQ=255)                                                                                                                       ttACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTTGCAACGGGGACTGGCTGt                           >  2:852375/1‑150 (MQ=255)                                                                                                                        tACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTTGGGGTATTCGCAACGGCGACCgg                               >  2:203143/1‑145 (MQ=255)                                                                                                                           cGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTaaaa                       <  1:517243/150‑3 (MQ=255)                                                                                                                            gTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACata                     <  2:943653/151‑1 (MQ=255)                                                                                                                              tAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACatat                    <  2:1186707/150‑1 (MQ=255)                                                                                                                              tAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGGTATTTGCAACGGCGACTGGCTGTAACata                     >  1:395698/1‑149 (MQ=255)                                                                                                                                aGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGGTATTCGCAACGGCGACTGGCTGTAACatat                    >  2:508869/1‑148 (MQ=255)                                                                                                                                  cgcCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTg               <  1:367586/151‑1 (MQ=255)                                                                                                                                     cTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTTGGGTTATTTGCAACGGCGACTGGGTGTAAAATATCTCTggg             >  2:287499/1‑148 (MQ=255)                                                                                                                                        aaaaaaCAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGGTATTCGCAAAGGCGACTGGCTGTAACATATCTCTGAGGcc          >  1:1142923/1‑150 (MQ=255)                                                                                                                                         aaaaaCAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACcgc        <  1:628741/151‑1 (MQ=255)                                                                                                                                              cAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGGTATTCCCAACGGCGACCGGCTGTAAAATATTTCTCAGACCCCGGTg    >  2:523546/1‑150 (MQ=255)                                                                                                                                               aGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATgcc  <  1:695213/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                 CGCGTCAGCGGGATGTCGATGAAAGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCC  >  NC_000913/4257454‑4257746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑