Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,486,3860GA41.5% 59.0 / 21.3 53A89A (GCG→GCA) crpcAMP‑activated global transcription factor, mediator of catabolite repression
Reads supporting (aligned to +/- strand):  ref base G (22/9);  new base A (0/22);  total (22/31)
Fisher's exact test for biased strand distribution p-value = 5.88e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTA‑TTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCG  >  NC_000913/3486244‑3486534
                                                                                                                                               |                                                                                                                                                    
acATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTA‑TTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGc                                                                                                                                              >  1:757244/1‑151 (MQ=255)
           ggCTCTGTGGCAGTGCTGAAAAAAGAAGAAGAGGGAAAAAAAATGACCCCCTCCTATCTGAATCAGGGTGATTTTA‑TTGGCGAACTGGGCCTGTTTGAAGAGGGCCGGAAACGTAGCGAAGGGGTACGTGCGAAAACCGCCTGTGAAGTgg                                                                                                                                   <  2:799801/151‑1 (MQ=255)
               ctGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTA‑TTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTg                                                                                                                                >  1:683185/1‑150 (MQ=255)
                   ggCAGTGTTGATAAAAGCGGAAGGGGGAAAAGAAATGTTCCTCTCCTACCGGAATCAGGGTGTTTTTA‑TTGGCGACCGGGGCTTGTTTGAAGAGGGCAAGGACCGTAGCCCATGGGTAGGTGCGAAAACCGCCTGTGAAGTGGCTg                                                                                                                                <  1:628345/146‑1 (MQ=255)
                   ggCAGTGCTAAAAAAAGAAGAAGAGGGAAAAGAAAGGACCCTCTCTTATCTAAACAAGGGTGTTTTTA‑TGGGCGAACTGGCCCTTTTGGAAGGGGGCCGGGAACGTGGCGATTGGGTGCGTGCAAAACCCGCCTGTGAAGTGGCTGaa                                                                                                                              <  1:760441/148‑1 (MQ=255)
                             aaaaaaGACGAGGAGGGTAAAGAAATGATCCTCCCCTATCTGAATCAGGGTGATTTTA‑TTGGCAAACGGGGCCTTTTGGAAGAGGGCAAGAACCGTAGCGCATGGGTACGTGAAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACaaaa                                                                                                                 <  1:538452/148‑1 (MQ=255)
                                 aaGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGAGATTTTA‑TTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAAt                                                                                                              >  2:1193243/1‑150 (MQ=255)
                                 aaGACGAAGAGGGTAAAGAAAGGATCCTCTCCTATCTAAAGCAGGGTGATTTTA‑TTGGCGACCTGGGCTTTTTTGAAGAGGGCCGGGAACGTAGCGCAGGGGTGCGGGCGAAACCCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAAtt                                                                                                             <  2:1307313/151‑1 (MQ=255)
                                    aagagggggaaaaaGAAGTAACCCTCTCCTATCTGAACCAGGGGTTTTTTA‑TGGGAGATCGGGGTTTGTTTGAAGGGGGACAGAAAGGTAGTGCTTGGGTACGGGAAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTc                                                                                                           <  1:1285418/139‑1 (MQ=255)
                                     cgacggggttAAAGAAGGCATCATCTCCTACCGGAATCAGGGTTTTTTTA‑TGGGCAACCTGGGCCTGTTTAAAAGGGCCCAGGACCGTAGCGAATGGGAACGTGCAAAACCCGCCTGTGAAGTGGCTGAAATTTCGTCCAAAAAATTTc                                                                                                           <  2:678249/140‑1 (MQ=255)
                                       aaGGGGATAAAAAAATGTTCCTCTCCTATCTGAATCAGGGTGTTTTTA‑TGGGCAAACTGGGCCTTTTTGAAGAGGGCAAGAAACGTAGCGAATGGGTGCGTGGAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTc                                                                                                           <  2:877480/147‑1 (MQ=255)
                                        agagGGTAAAGAAATGTTCCTCTCCTATCTGAATCAGGGGTTTTTTT‑TTGGCAAACGGCGCCGGTTAGAAGAGGCCAAGAAACGTGGCGCAGGGTTACGTGCAAAATCCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCaa                                                                                                      <  1:946682/151‑1 (MQ=255)
                                         gagGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGAGATTTTA‑TTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCa                                                                                                       >  1:1223902/1‑149 (MQ=255)
                                               aaaGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTA‑TTGGCGAACTGGGCCTTTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTCCGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTc                                                                                               <  2:1304288/151‑1 (MQ=255)
                                                aaGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTA‑TTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTc                                                                                               >  1:344698/1‑150 (MQ=255)
                                                          ccTCTCCTATCTGAATCAGGGTGATTTTA‑TGGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAAcc                                                                                      <  1:114014/149‑1 (MQ=255)
                                                                cTATCTGAATCAGGGTGATTTTA‑TTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACAtt                                                                              >  1:424474/1‑151 (MQ=255)
                                                                  aTCTGAATCAGGGTGATTTTA‑TTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCt                                                                            >  2:694067/1‑151 (MQ=255)
                                                                      gAATCAGGGT‑ATTTTATTTGGAGAGCGGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCAGGGGTACGTGCAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACAtt                                                                              <  2:608879/145‑1 (MQ=255)
                                                                       aaTCAGGTTGATTTTT‑TTGGCGAACGGGGCCTTTTTGAAGAGGGCAAGGAACGTAGCGCAGGGGTACGTGCAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTg                                                                           <  1:397532/147‑1 (MQ=255)
                                                                       aaTCAGGGTTATTTTA‑TTGGCGAACTGGGCCTGTTTGAAGGGGGCCAGAAACGTAGCGAATGGGTACGTGCAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACAtt                                                                              <  1:1266542/144‑1 (MQ=255)
                                                                       aaTCAGGGTGATTTTA‑TTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAAGGTAGCGCAGGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCt                                                                            <  2:407833/146‑1 (MQ=255)
                                                                       aaTCAGGGTGATTTTA‑TTGGCGAACGGGGCCTGTTTGAAGAGGGCCAGAAACGTAGCGAAGGGGTACGTGGAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTg                                                                           <  1:527353/147‑1 (MQ=255)
                                                                        aTCAGGGTGATTTTA‑TTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCg                                                                      >  1:1231751/1‑151 (MQ=255)
                                                                          cAGGGTGATTTTA‑TTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAAAGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGtt                                                                    >  1:1274474/1‑151 (MQ=255)
                                                                           aGGGTGATTTTA‑TTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGttt                                                                   >  2:444873/1‑151 (MQ=255)
                                                                                   tttA‑TTGGCGAACGGGGCCTTTTTGAAGAGGGCCAGGAACGTAGCGCAGGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGca                                                            <  1:946485/150‑1 (MQ=255)
                                                                                   tttA‑TTGGCAAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCAGGGGTACGTGCAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGca                                                            <  1:627850/150‑1 (MQ=255)
                                                                                    ttA‑TTGGCGACCGGGGCCTGTTTGAAGGGGGCCAGGAACGTAGCGCATGGGTACGTGCAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGcac                                                           <  1:1044523/150‑1 (MQ=255)
                                                                                    ttA‑TTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGcac                                                           >  2:487348/1‑150 (MQ=255)
                                                                                     tA‑TTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGcac                                                           >  1:126025/1‑149 (MQ=255)
                                                                                         tGGCGAACGGGGCCTGTTGGAGGAGGGCCAGGAACGTGGCGCATGGGTCCGTGCAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGAt                                                       <  1:472109/150‑1 (MQ=255)
                                                                                          ggCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACTTGGCGCATGGGTACGTGCAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATgg                                                     <  1:1252219/151‑1 (MQ=255)
                                                                                              aaCTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGgcg                                                   <  2:95779/149‑1 (MQ=255)
                                                                                                cTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGcgtc                                               >  2:538724/1‑151 (MQ=255)
                                                                                                  gggCTTGTTGAAAGAGGGCAGGGAACGTAGCGCATGGGTACGTGCAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGcgtcg                                              <  1:445748/150‑1 (MQ=255)
                                                                                                         tttGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCa                                        >  2:664234/1‑149 (MQ=255)
                                                                                                          ttGAAGGGGGCAAGGACCGTAGCGCGTGGGTCCGTGAAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCt                                           <  1:1319509/145‑1 (MQ=255)
                                                                                                          ttGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGt                                     >  1:1145045/1‑151 (MQ=255)
                                                                                                             aaGAGGGCGAGGAACGTGGCGCGGGGTTCCGGGCAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAg                                      <  1:853545/147‑1 (MQ=255)
                                                                                                                  ggCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTc                               >  2:1320000/1‑149 (MQ=255)
                                                                                                                     cAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGCTGGCGCGTCGTCTGCAAGTCACTTCag                             >  2:732838/1‑148 (MQ=255)
                                                                                                                           gggaGCGTAGGGGTAGGTGCAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTg                     <  1:1049074/147‑1 (MQ=255)
                                                                                                                           cGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTgg                    >  2:1123186/1‑151 (MQ=255)
                                                                                                                            gTAGCGCATGGTTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTgg                    <  2:870030/150‑1 (MQ=255)
                                                                                                                                cgcATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTggg                   <  2:151709/147‑1 (MQ=255)
                                                                                                                                cgcATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAAc               >  1:495213/1‑151 (MQ=255)
                                                                                                                                cgcATGGGTACGTGCAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCaa                <  1:1147419/150‑1 (MQ=255)
                                                                                                                                 gcATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAAcc              >  1:1174467/1‑151 (MQ=255)
                                                                                                                                   aTGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTg            >  1:679665/1‑151 (MQ=255)
                                                                                                                                     ggctACGGGCAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTgg           <  1:776893/147‑1 (MQ=255)
                                                                                                                                      ggTACGTGCAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCg         <  1:850425/151‑1 (MQ=255)
                                                                                                                                           gtcagAAACCCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCATCCTGGCGtt       <  2:1057131/144‑1 (MQ=255)
                                                                                                                                             gCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCg  >  2:738716/1‑151 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
ACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTA‑TTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCG  >  NC_000913/3486244‑3486534

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑