Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,732,811 G→T S1242I (AGC→ATC)  lhr → putative ATP‑dependent helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,732,8110GT98.9% 345.6 / ‑6.5 90S1242I (AGC→ATC) lhrputative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (43/46);  minor base C (0/1);  total (43/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACGGCCTGGTGGCACTGCATCTACAGGAATATGCTGCAGAATCGTTCACTCCCGCCGAAGCGGATCAGGCGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCAGCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGTTCCAACGCACGC  >  NC_000913/1732666‑1732956
                                                                                                                                                 |                                                                                                                                                 
gACGGCCTGGTGGCACTGCATCTACAGGAATATGCTGCAGAATCGTTCACTCCCGCCGAAGCGGATCAGGCGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAAc                                                                                                                                              <  2:971899/151‑1 (MQ=255)
gACGGCCTGGTGGCACTGCATCTACAGGAATATGCTGCAGAATCGTTCACTCCCGCCGAAGCGGATCAGGCGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAAc                                                                                                                                              >  1:507607/1‑151 (MQ=255)
gACGGCCTGGTGGCACTGCATCTACAGGAATATGCTGCAGAATCGTTCACTCCCGCCGAAGCGGATCAGGCGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAAc                                                                                                                                              >  2:795730/1‑151 (MQ=255)
  cGGCCTGGTGGCACTGCATCTACAGGAATATGCTGCAGAATCGTTCACTCCCGCCGAAGCGGATCAGGCGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACgg                                                                                                                                            >  1:178688/1‑151 (MQ=255)
   ggCCTGGTGGCACTGCATCTACAGGAATATGCTGCAGAATCGTTCACTCCCGCCGAAGCGGATCAGGCGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGa                                                                                                                                           <  1:219134/151‑1 (MQ=255)
    gCCTGGTGGCACTGCATCTACAGGAATATGCTGCAGAATCGTTCACTCCCGCCGAAGCGGATCAGGCGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGAt                                                                                                                                          >  2:1126502/1‑151 (MQ=255)
    gCCTGGTGGCACTGCATCTACAGGAATATGCTGCAGAATCGTTCACTCCCGCCGAAGCGGATCAGGCGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGAt                                                                                                                                          <  2:214137/151‑1 (MQ=255)
     ccTGGTGGCACTGCATCTACAGGAATATGCTGCAGAATCGTTCACTCCCGCCGAAGCGGATCAGGCGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATa                                                                                                                                         <  2:1040027/151‑1 (MQ=255)
     ccTGGTGGCACTGCATCTACAGGAATATGCTGCAGAATCGTTCACTCCCGCCGAAGCGGATCAGGCGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATa                                                                                                                                         <  1:300781/151‑1 (MQ=255)
      cTGGTGGCACTGCATCTACAGGAATATGCTGCAGAATCGTTCACTCCCGCCGAAGCGGATCAGGCGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATAc                                                                                                                                        >  2:994294/1‑151 (MQ=255)
       tggtggCACTGCATCTACAGGAATATGCTGCAGAATCGTTCACTCCCGCCGAAGCGGATCAGGCGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATAcg                                                                                                                                       >  1:526033/1‑151 (MQ=255)
       tggtggCACTGCATCTACAGGAATATGCTGCAGAATCGTTCACTCCCGCCGAAGCGGATCAGGCGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATAcg                                                                                                                                       >  2:1260849/1‑151 (MQ=255)
        ggtggCACTGCATCTACAGGAATATGCTGCAGAATCGTTCACTCCCGCCGAAGCGGATCAGGCGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATAcg                                                                                                                                       <  1:903359/150‑1 (MQ=255)
           ggCACTGCATCTACAGGAATATGCTGCAGAATCGTTCACTCCCGCCGAAGCGGATCAGGCGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGa                                                                                                                                    <  2:421346/150‑1 (MQ=255)
           ggCACTGCATCTACAGGAATATGCTGCAGAATCGTTCACTCCCGCCGAAGCGGATCAGGCGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGAc                                                                                                                                   <  1:597395/151‑1 (MQ=255)
            gCACTGCATCTACAGGAATATGCTGCAGAATCGTTCACTCCCGCCGATGCGGATCAGGCGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCACCCAACGGATACGCGa                                                                                                                                    <  2:1209243/149‑1 (MQ=255)
             cACTGCATCTACAGGAATATGCTGCAGAATCGTTCACTCCCGCCGAAGCGGATCAGGCGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACa                                                                                                                                  >  1:892878/1‑150 (MQ=255)
               cTGCATCTACAGGAATATGCTGCAGAATCGTTCACTCCCGCCGAAGCGGATCAGGCGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACaaa                                                                                                                                >  1:527526/1‑150 (MQ=255)
               cTGCAGCTACGGGAATATCCTGCAGAACCGTTCACTCCCGCCGAAGCGGATCAGGCGAATCGTTCGGCGCTCCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGACCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACaaa                                                                                                                                <  1:591130/150‑1 (MQ=255)
                     cTACAGGAATATGCTGCAGAATCGTTCACTCCCGCCGAAGCGGATCAGGCGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCg                                                                                                                         >  1:487844/1‑151 (MQ=255)
                       aCAGGAATATGCTGCAGAATCGTTCACTCCCGCCGAAGCGGATCAGGCGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGc                                                                                                                          <  1:1168648/148‑1 (MQ=255)
                       aCAGGAATATGCTGCAGAATCGTTCACTCCCGCCGAAGCGGATCAGGCGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCg                                                                                                                         <  2:1007050/149‑1 (MQ=255)
                         aGGAATATGCTGCAGAATCGTTCACTCCCGCCGAAGCGGATCAGGCGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAAt                                                                                                                      <  2:335228/150‑1 (MQ=255)
                              tatGCTGCAGAATCGTTCACTCCCGCCGAAGCGGATCAGGCGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTg                                                                                                                >  1:1208747/1‑151 (MQ=255)
                               atGCTGCAGAATCGTTCACTCCCGCCGAAGCGGATCAGGCGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTg                                                                                                                >  2:821082/1‑150 (MQ=255)
                                tgctgcAGAATCGTTCACTCCCGCCGAAGCGGATCAGGCGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGa                                                                                                               <  2:916469/150‑1 (MQ=255)
                                tgctgcAGAATCGTTCACTCCCGCCGAAGCGGATCAGGCGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGAt                                                                                                              >  2:1055007/1‑151 (MQ=255)
                                  ctgcAGAATCGTTCACTCCCGCCGAAGCGGATCAGGCGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGAtct                                                                                                            >  1:1077162/1‑151 (MQ=255)
                                  ctgcAGAATCGTTCACTCCCGCCGAAGCGGATCAGGCGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGAt                                                                                                              <  1:914166/149‑1 (MQ=255)
                                    gcAGAATCGTTCACTCCCGCCGAAGCGGATCAGGCGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGAtctc                                                                                                           <  1:1231743/150‑1 (MQ=255)
                                     cAGAATCGTTCACTCCCGCCGAAGCGGATCAGGCGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGAtctctc                                                                                                         >  2:84927/1‑151 (MQ=255)
                                            gTTCACTCCCGCCGAAGCGGATCAGGCGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGccc                                                                                                    <  1:261185/149‑1 (MQ=255)
                                            gTTCACTCCCGCCGAAGCGGATCAGGCGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTg                                                                                                  >  1:618529/1‑151 (MQ=255)
                                             ttCACTCCCGCCGAAGCGGATCAGGCGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTg                                                                                                  <  1:356518/150‑1 (MQ=255)
                                              tCACTCCCGCCGAAGCGGATCAGGCGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGc                                                                                                 >  2:878260/1‑150 (MQ=255)
                                                 cTCCCGCCGAAGCGGATCAGGCGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCa                                                                                                >  2:143711/1‑148 (MQ=255)
                                                 cTCCCGCCGAAGCGGATCAGGCGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCa                                                                                                >  2:299998/1‑148 (MQ=255)
                                                    ccgccgAAGCGGATCAGGCGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGc                                                                                          <  2:844329/151‑1 (MQ=255)
                                                        cgAAGCGGATCAGGCGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGtt                                                                                       >  2:1025517/1‑150 (MQ=255)
                                                              ggATCAGGCGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGgcg                                                                                >  1:1206735/1‑151 (MQ=255)
                                                              ggATCAGGCGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGgcg                                                                                <  1:278925/151‑1 (MQ=255)
                                                                aTCAGGCGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCt                                                                              <  1:586112/151‑1 (MQ=255)
                                                                 tCAGGCGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTg                                                                             >  1:297019/1‑151 (MQ=255)
                                                                  cAGGCGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGAGCTg                                                                             >  2:1191737/1‑150 (MQ=255)
                                                                   aGGCGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTg                                                                             <  2:756087/149‑1 (MQ=255)
                                                                      cGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTg                                                                        >  1:643198/1‑151 (MQ=255)
                                                                      cGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTg                                                                        >  2:1065877/1‑151 (MQ=255)
                                                                        aaTCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGc                                                                      <  2:73683/151‑1 (MQ=255)
                                                                          tCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCa                                                                     >  1:798846/1‑150 (MQ=255)
                                                                           cGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCa                                                                     >  1:224483/1‑149 (MQ=255)
                                                                            gTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAgg                                                                  >  1:71492/1‑151 (MQ=255)
                                                                               cGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCg                                                                <  1:1059793/150‑1 (MQ=255)
                                                                                ggCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGtc                                                              <  2:924260/151‑1 (MQ=255)
                                                                                      gCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCAcc                                                        >  1:1205000/1‑151 (MQ=255)
                                                                                         acaaGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCTGc                                                     <  1:1194621/151‑1 (MQ=255)
                                                                                         acaaGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGc                                                     <  1:1126441/151‑1 (MQ=255)
                                                                                          caaGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCg                                                    <  1:654697/151‑1 (MQ=255)
                                                                                             gCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACa                                                 <  2:465652/151‑1 (MQ=255)
                                                                                              cGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACAt                                                <  1:290942/151‑1 (MQ=255)
                                                                                                aTAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACAttt                                              <  1:163036/151‑1 (MQ=255)
                                                                                                  aGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTg                                             <  2:1168820/150‑1 (MQ=255)
                                                                                                   gTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTggg                                           >  2:1227000/1‑151 (MQ=255)
                                                                                                    tCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCCAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGc                                          >  1:701810/1‑151 (MQ=255)
                                                                                                    tCGCTGTTCTGGCTGACGGAGCCGCCTGGATTGCACAACAAATCATCCAACGGCTACGCGACAAAATCGGCGACTCGGTTGATCTCTCCGCCCCGCAAGACGCGTTATGGGCGCTCGGCTGGCACGGCGCCCTCACCAGCGACATTTGGGc                                          >  1:917024/1‑151 (MQ=255)
                                                                                                     cGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGc                                          <  2:508054/150‑1 (MQ=255)
                                                                                                        tGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCg                                      >  1:645814/1‑151 (MQ=255)
                                                                                                         gTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCAcc                                       <  1:167791/149‑1 (MQ=255)
                                                                                                          ttCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGtt                                    >  1:250026/1‑151 (MQ=255)
                                                                                                           tCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGtt                                    <  1:927533/150‑1 (MQ=255)
                                                                                                           tCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGtt                                    <  2:457936/150‑1 (MQ=255)
                                                                                                             tGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTAcg                                 >  1:902522/1‑151 (MQ=255)
                                                                                                              ggCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTAc                                  <  2:746557/149‑1 (MQ=255)
                                                                                                               gCTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTAcgcg                               <  1:297076/151‑1 (MQ=255)
                                                                                                                cTGACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACgcgc                              >  2:286113/1‑151 (MQ=255)
                                                                                                                  gACGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGccc                            <  2:855186/151‑1 (MQ=255)
                                                                                                                    cGGAGGAGCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTc                          >  1:408853/1‑151 (MQ=255)
                                                                                                                      gaggagCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCAc                        <  1:586172/151‑1 (MQ=255)
                                                                                                                       aggagCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCAcc                       >  2:57068/1‑151 (MQ=255)
                                                                                                                       aggagCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCAcc                       <  2:597315/151‑1 (MQ=255)
                                                                                                                       aggagCCTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCAcc                       >  2:911735/1‑151 (MQ=255)
                                                                                                                             cTGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCgcagc                 >  1:598281/1‑151 (MQ=255)
                                                                                                                              tGGTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCgcagc                 >  2:996814/1‑150 (MQ=255)
                                                                                                                               ggTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCgcagca                <  1:133095/150‑1 (MQ=255)
                                                                                                                               ggTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCgcagca                <  1:100004/150‑1 (MQ=255)
                                                                                                                                gTTTGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGt              <  1:754794/151‑1 (MQ=255)
                                                                                                                                 tttGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGtt             >  1:218138/1‑151 (MQ=255)
                                                                                                                                  ttGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGtt             <  1:339308/150‑1 (MQ=255)
                                                                                                                                  ttGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGtt             <  1:717925/150‑1 (MQ=255)
                                                                                                                                   tGCACAACAAATCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGTTc            <  2:1008698/150‑1 (MQ=255)
                                                                                                                                            aaTCATCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGTTCCAacgcacgc  >  2:143694/1‑151 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
GACGGCCTGGTGGCACTGCATCTACAGGAATATGCTGCAGAATCGTTCACTCCCGCCGAAGCGGATCAGGCGAATCGTTCGGCGCTGCAACAAGCGATAGTCGCTGTTCTGGCTGACGGAGGAGCCTGGTTTGCACAACAAATCAGCCAACGGATACGCGACAAAATCGGCGAATCGGTTGATCTCTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGTTCCAACGCACGC  >  NC_000913/1732666‑1732956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: