Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,744,313 T→A V286E (GTG→GAG)  mdtK → multidrug efflux system transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,744,3130TA98.6% 271.6 / ‑6.3 73V286E (GTG→GAG) mdtKmultidrug efflux system transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (36/36);  minor base G (1/0);  total (37/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

GACTGATTCAACTCGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTT  >  NC_000913/1744166‑1744459
                                                                                                                                                   |                                                                                                                                                  
gACTGATTCAACTCGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCt                                                                                                                                                 >  2:1190798/1‑151 (MQ=255)
    gATTCAACTCGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCa                                                                                                                                             <  1:1072571/151‑1 (MQ=255)
      ttCAACTCGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGAAACTGTTTGACGTCGTGGCTCTGTTAGTGTCTGCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTcga                                                                                                                                             <  1:826913/149‑3 (MQ=255)
       tCAACTCGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAAt                                                                                                                                           <  1:595076/150‑1 (MQ=255)
       tCAACTCGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATg                                                                                                                                          >  2:1176490/1‑151 (MQ=255)
       tCAACTCGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATg                                                                                                                                          >  2:1166490/1‑151 (MQ=255)
         aaCTCGGTTTGCGGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGCATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGTTTCCAATGTc                                                                                                                                        >  1:1102628/1‑151 (MQ=255)
         aaCTCGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTc                                                                                                                                        <  1:7581/151‑1 (MQ=255)
             cGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGc                                                                                                                                      <  2:481008/149‑1 (MQ=255)
             cGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCt                                                                                                                                     <  2:225712/150‑1 (MQ=255)
             cGGTTTGCCGATTGCGCTGGCACTGTTCTTCGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTg                                                                                                                                    <  2:691817/151‑1 (MQ=255)
                   gCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTggcggc                                                                                                                               >  2:1205517/1‑150 (MQ=255)
                   gCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTggcggc                                                                                                                               <  1:844103/150‑1 (MQ=255)
                      gATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCg                                                                                                                           >  2:807103/1‑151 (MQ=255)
                        ttGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGt                                                                                                                         >  2:81637/1‑151 (MQ=255)
                        ttGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGt                                                                                                                         >  1:754879/1‑151 (MQ=255)
                         tGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGt                                                                                                                         <  1:1098635/150‑1 (MQ=255)
                         tGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGt                                                                                                                         >  1:241345/1‑150 (MQ=255)
                           cgcTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAAc                                                                                                                      <  1:980114/151‑1 (MQ=255)
                            gcTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACt                                                                                                                     >  2:564685/1‑151 (MQ=255)
                            gcTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACt                                                                                                                     >  1:982408/1‑151 (MQ=255)
                               ggCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTTGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTAt                                                                                                                   <  2:1084421/150‑1 (MQ=255)
                                  aCTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTAACcgc                                                                                                               >  1:479/1‑151 (MQ=255)
                                   cTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCcgc                                                                                                               <  1:721221/150‑1 (MQ=255)
                                       tCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGGTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAg                                                                                                           <  1:453913/150‑1 (MQ=255)
                                         tttGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGtt                                                                                                        >  1:465225/1‑151 (MQ=255)
                                          ttGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGt                                                                                                         <  2:811637/149‑1 (MQ=255)
                                           tGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTa                                                                                                       <  1:553514/150‑1 (MQ=255)
                                            gAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGGTCGGTATTGTTGATGTCGCAGGTCACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAACTTCCAATGTCGCTGGCTGCAGCGGTAACTATCCGCGGAGGGTAt                                                                                                      >  2:342492/1‑150 (MQ=255)
                                            gAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATc                                                                                                     <  2:586462/151‑1 (MQ=255)
                                              aGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCg                                                                                                    >  2:559429/1‑150 (MQ=255)
                                              aGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGt                                                                                                   <  1:963870/151‑1 (MQ=255)
                                                 gACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCt                                                                                                 <  1:316501/150‑1 (MQ=255)
                                                    acTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTggg                                                                                              >  1:364478/1‑150 (MQ=255)
                                                    acTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGt                                                                                             >  2:408901/1‑151 (MQ=255)
                                                       gTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTc                                                                                            <  1:891191/149‑1 (MQ=255)
                                                         ttGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAggg                                                                                        >  2:999100/1‑151 (MQ=255)
                                                          tGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAggg                                                                                        >  1:397681/1‑150 (MQ=255)
                                                           gCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGc                                                                                       >  1:857251/1‑150 (MQ=255)
                                                           accGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGc                                                                                       >  1:379782/2‑150 (MQ=255)
                                                                   ggCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGGTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCt                                                                               <  2:424516/150‑1 (MQ=255)
                                                                   ggCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCt                                                                               <  1:906415/150‑1 (MQ=255)
                                                                   ggCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTg                                                                              >  1:369194/1‑151 (MQ=255)
                                                                       ctGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGa                                                                            >  1:212015/1‑149 (MQ=255)
                                                                          ttAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATgcgc                                                                       >  2:871392/1‑151 (MQ=255)
                                                                                   ccGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCt                                                               <  2:228583/150‑1 (MQ=255)
                                                                                        cGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGcgcg                                                          <  2:181305/150‑1 (MQ=255)
                                                                                        cGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACCAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCgg                                                         >  1:907715/1‑151 (MQ=255)
                                                                                            aTTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGAcc                                                      <  2:398472/150‑1 (MQ=255)
                                                                                               gttgATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGc                                                  <  2:493483/151‑1 (MQ=255)
                                                                                               gttgATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGc                                                  >  2:867728/1‑151 (MQ=255)
                                                                                                         cAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATggtggg                                        >  1:1181468/1‑151 (MQ=255)
                                                                                                          aGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATggtgggt                                       <  2:418117/151‑1 (MQ=255)
                                                                                                             acacCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATggtgggtggc                                    >  1:58796/1‑149 (MQ=255)
                                                                                                               acCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTg                                  >  1:487112/1‑151 (MQ=255)
                                                                                                                ccAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGt                                 <  2:691064/151‑1 (MQ=255)
                                                                                                                 cAGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGt                                 >  1:376536/1‑150 (MQ=255)
                                                                                                                  aGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAATCCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTAt                               <  1:134292/151‑1 (MQ=255)
                                                                                                                  aGATTGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTAt                               <  2:284985/151‑1 (MQ=255)
                                                                                                                     ttGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGc                            >  2:481490/1‑151 (MQ=255)
                                                                                                                      tGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGc                            <  2:659499/150‑1 (MQ=255)
                                                                                                                      tGCCCTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGc                            >  1:1215670/1‑150 (MQ=255)
                                                                                                                        cccTGAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAAc                         <  2:70733/151‑1 (MQ=255)
                                                                                                                            gAACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCt                      <  2:58797/150‑1 (MQ=255)
                                                                                                                              aCTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGAc                   >  2:523733/1‑151 (MQ=255)
                                                                                                                              aCTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGAc                   <  2:966442/151‑1 (MQ=255)
                                                                                                                                      tCACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCAtt            <  1:766753/150‑1 (MQ=255)
                                                                                                                                       cACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCAttt           <  2:468419/150‑1 (MQ=255)
                                                                                                                                       cACTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCAttt           <  1:1022947/150‑1 (MQ=255)
                                                                                                                                         cTAATGTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTc         >  2:257360/1‑150 (MQ=255)
                                                                                                                                            aTGTTCGGGTTTCCAATGTCGCTGGCCCCAGCGGTAACTATCCGCGTAGGATCTCGTATGGGTCAGGGCTCAACGCTGGATGCGCCAACCTCTGCGCCAACCGGGCTTCTGGTTTGGGACTGAATGGCCAGCCTAACGGCCATTTTCACgg     >  1:1102028/1‑151 (MQ=255)
                                                                                                                                              gTTCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGtt   >  1:682799/1‑151 (MQ=255)
                                                                                                                                               ttCGAGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGttt  >  2:136127/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GACTGATTCAACTCGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTT  >  NC_000913/1744166‑1744459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: