breseq version 0.29.0 revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 564 shown) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 357,890 | 0 | C | A | 56.7% | 1.2 / 35.4 | 30 | L267F (TTG→TTT) | cynR | transcriptional activator of cyn operon; autorepressor |
* | NC_000913 | 599,576 | 0 | T | G | 50.0% | 64.2 / 38.2 | 66 | V288G (GTG→GGG) | cusA | copper/silver efflux system, membrane component |
* | NC_000913 | 3,508,662 | 0 | T | A | 45.0% | 80.5 / 28.0 | 62 | E384D (GAA→GAT) | yhfW | phosphopentomutase‑related metalloenzyme superfamily protein |
* | NC_000913 | 3,177,531 | 0 | A | C | 44.9% | 133.3 / 18.9 | 91 | V127G (GTG→GGG) | nudF | ADP‑ribose pyrophosphatase |
* | NC_000913 | 2,678,039 | 0 | T | G | 44.1% | 85.5 / 15.0 | 62 | N108T (AAC→ACC) | yphE | putative sugar ABC transporter ATPase |
* | NC_000913 | 3,508,657 | 0 | A | G | 43.5% | 88.5 / 14.9 | 62 | F386S (TTC→TCC) | yhfW | phosphopentomutase‑related metalloenzyme superfamily protein |
* | NC_000913 | 17,050 | 0 | A | C | 43.1% | 106.0 / 13.9 | 73 | intergenic (‑90/‑439) | mokC/nhaA | regulatory protein for HokC, overlaps CDS of hokC/sodium‑proton antiporter |
* | NC_000913 | 3,026,941 | 0 | A | C | 40.5% | 126.0 / 16.2 | 79 | E392D (GAA→GAC) | guaD | guanine deaminase |
* | NC_000913 | 3,812,792 | 0 | C | A | 40.3% | 128.8 / 32.1 | 72 | T21N (ACC→AAC) | dfp | fused 4'‑phosphopantothenoylcysteine decarboxylase/phosphopantothenoylcysteine synthetase, FMN‑binding |
* | NC_000913 | 4,378,802 | 0 | A | C | 40.0% | 89.8 / 21.1 | 55 | V48G (GTA→GGA) | ampC | penicillin‑binding protein; beta‑lactamase, intrinsically weak |
* | NC_000913 | 1,080,837 | 0 | G | T | 39.4% | 105.6 / 32.6 | 71 | intergenic (+24/‑519) | putP/efeU | proline:sodium symporter/ferrous iron permease (pseudogene) |
* | NC_000913 | 599,579 | 0 | T | G | 39.4% | 106.3 / 11.5 | 66 | V289G (GTG→GGG) | cusA | copper/silver efflux system, membrane component |
* | NC_000913 | 1,435,727 | 0 | G | A | 39.2% | 109.8 / 55.4 | 79 | intergenic (‑108/+33) | uspF/ompN | stress‑induced protein, ATP‑binding protein/outer membrane pore protein N, non‑specific |
* | NC_000913 | 3,556,207 | 0 | T | C | 39.2% | 111.6 / 22.8 | 74 | S215G (AGT→GGT) | rtcA | RNA 3'‑terminal phosphate cyclase |
* | NC_000913 | 4,457,105 | 0 | A | G | 39.2% | 105.3 / 27.4 | 74 | I441V (ATC→GTC) | mpl | UDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase |
* | NC_000913 | 2,168,966 | 0 | A | C | 38.2% | 104.4 / 17.8 | 69 | I85I (ATA→ATC) | yegS | phosphatidylglycerol kinase, metal‑dependent |
* | NC_000913 | 3,350,844 | 0 | A | C | 38.2% | 121.4 / 18.0 | 68 | L728V (TTA→GTA) | arcB | aerobic respiration control sensor histidine protein kinase, cognate to two‑component response regulators ArcA and RssB |
* | NC_000913 | 2,326,450 | 0 | T | G | 38.2% | 102.9 / 13.4 | 56 | G114G (GGT→GGG) | atoB | acetyl‑CoA acetyltransferase |
* | NC_000913 | 4,604,344 | 0 | C | T | 38.2% | 73.3 / 23.9 | 55 | S62F (TCT→TTT) | bglJ | bgl operon transcriptional activator |
* | NC_000913 | 4,604,350 | 0 | C | T | 38.2% | 70.6 / 26.0 | 55 | S64F (TCT→TTT) | bglJ | bgl operon transcriptional activator |
Marginal new junction evidence (sorted from low to high skew) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | = 1207805 | 71 (0.880) | 14 (0.200) | 14/266 | 8.0 | 19.1% | coding (305/630 nt) | stfP | e14 prophage; uncharacterized protein |
? | NC_000913 | = 1209602 | 55 (0.770) | pseudogene (18/501 nt) | stfE | pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related | |||||
* | ? | NC_000913 | 1207790 = | 74 (0.920) | 8 (0.110) | 8/266 | 11.0 | 11.4% | coding (290/630 nt) | stfP | e14 prophage; uncharacterized protein |
? | NC_000913 | 1209619 = | 58 (0.810) | pseudogene (1/501 nt) | stfE | pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related |