Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913907,2180GA40.4% 56.6 / 26.7 52F355F (TTC→TTTybjTputative NAD‑dependent oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (20/11);  new base A (0/21);  total (20/32)
Fisher's exact test for biased strand distribution p-value = 8.91e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGTTGTTTTTCCGGTTCAACGACAATCACTTTCCAGCTATCCACCGCATCGCCAGTCTGTAAATATTCGCGCTCCGGGCGGCCTTTCGCCAGCTTATGACCGATCGCGCGGTCCATCAACGCCCGTGTCTGCCACAAAATATTGCCAAAGAAATAACGCTCTTTACCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCAAAGGCCTGAGCGTCGTAGCCCCAGTC  >  NC_000913/907068‑907367
                                                                                                                                                        |                                                                                                                                                         
aaGTTGTTTTTCCGGTTCAACGACAATCACTTTCCAGCTATCCACCGCATCGCCAGTCTGTAAATATTCGCGCTCCGGGCGGCCTTTCGCCAGCTTATGACCGATCGCGCGGTCCATCAACGCCCGTGTCTGCCACAAAATATTGCCAAAg                                                                                                                                                           <  2:674258/151‑1 (MQ=255)
     gttTTTCCGGTTCAACGACAATCACTTTCCAGCTATCCACCGCATCGCCAGTCTGTAAATATTCGCGCTCCGGGCGGCCTTTCGCCAGCTTATGACCGATCGCGCGGTCCATCAACGCCCGTGTCTGCCACAAAATATTGCCAAAGAAAt                                                                                                                                                      >  2:224642/1‑150 (MQ=255)
      tttttCCGGTTCAACGACAATCACTTTCCAGCTATCCACCGCATCGCCAGTCTGTAAATATTCGCGCTCCGGGCGGCCTTTCGCCAGCTTATGACCGATCGCGCGGTCCATCAACGCCCGTGTCTGCCACAAAATATTGCCAAAGAAATa                                                                                                                                                     >  1:992827/1‑150 (MQ=255)
                  aaCGACAATCACTTTCCAGCTATCCACCGCATCGCCAGTCTGTAAATATTCGCGCTCCGGGCGGCCTTTCGCCAGCTTATGACCGATCGCGCGGTCCATCAACGCCCGTGTCTGCCACAAAATATTGCCAAAGAAATAACGCTCTTTAccg                                                                                                                                     >  2:87397/1‑151 (MQ=255)
                   aCGACAATCACTTTCCAGCTATCCACCGCATCGCCAGTCTGTAAATATTCGCGCTCCGGGCGGCCTTTCGCCAGCTTATGACCGATCGCGCGGTCCATCAACGCCCGTGTCTGCCACAAAATATTGCCAAAGAAATAACGCTCTTTAccgc                                                                                                                                    <  2:311338/151‑1 (MQ=255)
                      aCAATCACTTTCCAGCTATCCACCGCATCGCCAGTCTGTAAATATTCGCGCTCCGGGCGGCCTTTCGCCAGCTTATGACCGATCGCGCGGTCCATCAACGCCCGTGTCTGCCACAAAATATTGCCAAAGAAATAACGCTCTTTAccgccg                                                                                                                                  >  2:723478/1‑150 (MQ=255)
                       cAATCACTTTCCAGCTATCCACCGCATCGCCAGTCTGTAAATATTCGCGCTCCGGGCGGCCTTTCGCCAGCTTATGACCGATCGCGCGGTCCATCAACGCCCGTGTCTGCCACAAAATATTGCCAAAGAAATAACGCTCTTTAccgccg                                                                                                                                  <  2:62952/149‑1 (MQ=255)
                           cACTTTCCAGCTACCCCCCGCATCGCCAGTCTGTAAATATTCGCGCTCCGGGCGGCCTTTCGCCAGCTTATGACCGATCCCGCGGTCCATCAACGCCCGTGTCTGCCACAAAATATTGCCAAAGAAATAACGCTCTTTACCGCCGAttt                                                                                                                              <  1:1094115/149‑1 (MQ=255)
                             cTTTCCAGCTATCCCCCGCATCGCCAGTCTGTAAATATTCGCGCTCCGGGCGGCCTTTCGCCAGCTTATGACCGATCGCGCGGTCCATCAACGCCCGTGTCTGCCACAAAATATTGCCAAAGAAATAACGCTCTTTACCGCCGATTTGGt                                                                                                                           <  1:297225/150‑1 (MQ=255)
                                    gCTATCCACCGCATCGCCAGTCTGTAAATATTCGCGCTCCGGGCGGCCTTTCGCCAGCTTATGACCGATCGCGCGGTCCATCAACGCCCGTGTCTGCCACAAAATATTGCCAAAGAAATAACGCTCTTTACCGCCGATTTGGTTCACTAcc                                                                                                                   >  2:834625/1‑151 (MQ=255)
                                       aTCCACCGCATCGCCAGTCTGTAAATATTCGCGCTCCGGGCGGCCTTTCGCCAGCTTATGACCGATCGCGCGGTCCATCAACGCCCGTGTCTGCCACAAAATATTGCCAAAGAAATAACGCTCTTTACCGCCGATTTGGTTCACTACCt                                                                                                                  <  1:117561/149‑1 (MQ=255)
                                       aTCCACCGCATCGCCAGTCTGTAAATATTCGCGCTCCGGGCGGCCTTTCGCCAGCTTATGACCGATCGCGCGGTCCATCAACGCCCGTGTCTGCCACAAAATATTGCCAAAGAAATAACGCTCTTTACCGCCGATTTGGTTCACTACCTGc                                                                                                                >  1:1238932/1‑151 (MQ=255)
                                         ccACCGCATCGCCAGTCTGTAAATATTCGCGCTCCGGGCGGCCTTTCGCCAGCTTATGACCGATCGCGCGGCCCATCAACGCCCGTGTCTGCCACAAAATATTGCCAAAGAAATAACGCTCTTTACCGCCGATTTGGTTCACTACCTGcc                                                                                                               <  2:836582/150‑1 (MQ=255)
                                                  cGCCAGTCTGTAAATATTCGCGCTCCGGGCGGCCTTTCGCCAGCTTATGACCGATCGCGCGGTCCATCAACGCCCGTGTCTGCCACAAAATATTGCCAAAGAAATAACGCTCTTTACCGCCGATTTGGTTCACTACCTGCCATAAagcagc                                                                                                     >  1:1241742/1‑151 (MQ=255)
                                                           gTAAATATTCGCGCTCCGGGCGGCCTTTCGCCAGCTTATGACCGATCGCGCGGTCCATCAACGCCCGTGTCTGCCACAAAATATTGCCAAAGAAATAACGCTCTTTACCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTgg                                                                                             >  1:193713/1‑150 (MQ=255)
                                                             aaatttcccccccccGGGCGCCTTTTCGCCAGCTTATGCCCGACCGCCCTGCCCATCACCCCCCGTGTCTCCCACAAAATATGGCCAAAAAAAAACCGCTTTTTCCCGCAAATTTGGTTCACTCCCTGCCATAAAGCAGCAAGGCTg                                                                                              <  1:913513/134‑1 (MQ=255)
                                                             aaaTTTTCGCCCTCCGGGCGCCTTTTCACCACTTTACGACCGATCGCGGGTCCCACCACCGCCCGGTTCTGCCAAAAAATATTCCCAAAAAAAAAACCCTCTTTACCGCCGATTTGGTTCACTCCCTGCCATAAAGCAGCAAGGCTGGc                                                                                            <  1:482223/149‑1 (MQ=255)
                                                             aaaTATTCGCCCTCCGGGCGGCCTTTCGCCAGCTTATGACCGATCGCGCGGTCCATCAACGCCCGTGTCTGCCACAAAATATTGCCAAAGAAATAACGCTCTTTCCCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTgg                                                                                             <  2:10893/148‑1 (MQ=255)
                                                                    cgccccccGGGCGGCCTTCCGCCACCTTATGACCGAGCGCGCGGCCCATCAACGCCCGTGTCTGCCACAAAATATTGCCAAAGAAATAACGCCTTTTCCCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGtttt                                                                                   <  2:18413/145‑1 (MQ=255)
                                                                      cgcCCCGGGCGCCTTTCCCCCACCTAATAACCAACCGCGCGATCAATAACCGCCTGTGCCCGCAACAAAATTTTCCCAAAAAAAAAACGCTCTTACCCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTa                                                                                  <  1:802610/150‑1 (MQ=255)
                                                                        cTCCGGGCGGCCTTTCGCCAGCTTATGACCGATCGCGCGGTCCATCAACGCCCGTGTCTGCCACAAAATATTGCCAAAGAAATAACGCTCTTTACCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACg                                                                               >  2:461368/1‑151 (MQ=255)
                                                                          ccGGGCGGCCTTTCGCCAGCTTATGACCGATCGCGCGGTCCATCAACGCCCGTGTCTGCCACAAAATATTGCCAAAGAAATAACGCTCTTTACCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACg                                                                               >  2:800845/1‑149 (MQ=255)
                                                                                ggcCTTTCGCCAGCTTATGACCGATCGCGCGGTCCATCAACGCCCGTGTCTGCCACAAAATATTGCCAAAGAAATCACGCTCTTTACCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCACGGCTGGCGGACGTTTTAACGGTAAAccc                                                                       >  1:317844/1‑151 (MQ=255)
                                                                                        gCCAGCTTATGACCGATCGCGCGGTCCATCAACGCCCGTGTCTGCCACAAAATATTGCCAAAGAAATAACGCTCTTTACCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCACGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGtt                                                               >  2:1094452/1‑151 (MQ=255)
                                                                                         ccgctttatgccataTCGGGGGGCCCACCACCCCCGGTGTTTGCCAAAAAATATGCCAAAAAAAATAACGCTCTTTCCCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGttt                                                              <  2:990748/137‑1 (MQ=255)
                                                                                           accttaggccgacccgcgcgGCCCATCACCCCCCGTGTCTGCCAAAAAATATCCCAAAAGAAAAACCGCTTTTTACCGCCGATTTGGTTCACTCCCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGtt                                                               <  1:474960/134‑1 (MQ=255)
                                                                                               ttatgccggtccgccgGTCCAACCACCCCCCTTTTTGCCAAAAAAATTTGCCAAAAAAAAAACCCCTTTTTCCCGCCGATTTGGTTCACTCCCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGttt                                                              <  1:1104042/132‑1 (MQ=255)
                                                                                                aggcccGATCGCGGGGCCCATCACCCCCCGTGTCTGCCAAAAAATATTGCAAAAAAAAAACCGCTCTTTACCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCaa                                                         <  2:1066055/145‑1 (MQ=255)
                                                                                                  gACCGATCGCGCGGTCCATCAACGCCCGTGTCTGCCACAAAATATTGCCAAAGAAATAACGCTCTTTACCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAAt                                                      >  1:30235/1‑150 (MQ=255)
                                                                                                    ccGCTCGCGCGTCCAAAAACCCCCGTTGTCTGCCAAAAAATTTTCCAAAAAAAAAACCGCTTTTTCCCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAAt                                                      <  1:1103101/148‑1 (MQ=255)
                                                                                                    ccGACCGCGCGCTCCACCACCGCCCGTGTCTCCCAAAAAATTTTCCAAAAAAAAAACCGCTTTTTCCCGCCGTTTTGGTCCCCTCCCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAAc                                                   <  1:1134700/151‑1 (MQ=255)
                                                                                                      gaccgcgcgCTCCAACACCCCCCGTGTCCCCCAAAAAATTTTCCAAAAAAAAAACCCCTTTTACCCGCCGTTTTGGTTCACTCCCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCg                                                 <  1:328414/148‑1 (MQ=255)
                                                                                                           cgcgGTCCATCAACGCCCGTGTCTGCCACAAAATATTGCCAAAGAAATAACGCTCTTTACCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTc                                            >  2:415801/1‑151 (MQ=255)
                                                                                                              gctccacaaccGCCGGTGTCTGCCGCAAATTATTACAAAAAAAAAACCCCCTTTACCCCCCGATTCGGTTCCCTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCg                                                 <  2:829544/133‑1 (MQ=255)
                                                                                                               gTCCATCAACGCCCGTGTCTGCCACAAAATATTGCCAAAGAAATAACGCTCTTTACCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCgg                                         >  2:12755/1‑150 (MQ=255)
                                                                                                               acccATCACCCCGCGTGTCTCCCAAAAAACATTCCAAAAAAAATAACGCTTTTACCCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCgg                                         <  2:167772/148‑1 (MQ=255)
                                                                                                                 cccacctcGCCGGTTTCTCTCACAAAAATTTGCAAAGAATATATCCCCTTTTCCCGCCCTTTTGGTCCACTTCCTGCCATAATGCAGCAAGCCTGTCGCACGTTTTAACGTTAATCCCCGCCTGTCTGGCAAAATAACCGTACTCCGGTCg                                      <  1:656247/144‑1 (MQ=255)
                                                                                                                      acccccggtgtCCCCCAAAAAATTTTGCAAAAAAAAAACCCCTTTTCCCCGCCGTTTTGGTTCACTCCCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGc                                     <  1:268335/140‑1 (MQ=255)
                                                                                                                      accGCCCGTTTCTCCCAAAAATTATTGCAAAAAAAAAACCGCTCTTTCCCGCCGATTTGTTTCACTCCCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCa                                   <  2:748989/147‑1 (MQ=255)
                                                                                                                      accGCCCGTGTTGCCCAAAAAATTTGCCAAAAGAAAGACCCCTTTTCCCCGCCGTTTTGGTTCACTCCCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCg                                      <  1:442866/144‑1 (MQ=255)
                                                                                                                       aCGCCCGTGTCTGCCACAAAATATTGCCAAAGAAATAACGCTCTTTACCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCg                                >  2:469157/1‑151 (MQ=255)
                                                                                                                           ccgggttCTGCCAAAAAATTTGCCAAAAAAAATACCCCTTTTTCCCCCCTTTTTGTTTCCCTCCCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTc                                            <  1:983356/130‑1 (MQ=255)
                                                                                                                                   gcccAAAAAATAGGGCAAAAAAAAAAGCCCTTTTCCCCGCCAATTGGGTTCACTCCCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCAAAg                         <  1:1228453/145‑1 (MQ=255)
                                                                                                                                     ccAAAAAATATTCCAAAAAAAAAAACGCTTTTTCCCGCCGATTTGGTTCCCTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCAAAGGCCt                     <  2:453004/149‑1 (MQ=255)
                                                                                                                                     ccAAAAAATATGCCAAAAAAAAAAACCCTTTTTCCCGCCGATTTGGTTCACTCCCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCAAAGGCCt                     <  2:665496/149‑1 (MQ=255)
                                                                                                                                        aaaaaatttGCCAAAAAAAATACCGCGCTTTCCCCCCGATTTGGTTCCCTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCAAAGGCCTGAGc                 <  1:167403/144‑1 (MQ=255)
                                                                                                                                        aaaaaTATTGCAAAAAAAAAACCCCTTTTTCCCGCCGTTTTGGTTCACTCCCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCAAAGGCCTGAGcg                <  2:1010570/150‑1 (MQ=255)
                                                                                                                                         aaaaatttGCCAAAAAAAAAACCCCTCTTCACCGCCGATTTGGTTCACTCCCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCAAAGGCCTGAGcg                <  1:435250/145‑1 (MQ=255)
                                                                                                                                          aaaTATTGCCAAAGAAATAACGCTCTTTACCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCAAAGGCCTGAGcgtc              >  2:764043/1‑151 (MQ=255)
                                                                                                                                             tatTGCCAAAGAAATAACGCTCTTTACCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCAAAGGCCTGAGcgtcgt            >  2:1036887/1‑150 (MQ=255)
                                                                                                                                                   cAAAAAAATAACCGTCTTTCCCCGCGGATTTGGTTCTCTCCCTGCCATAAAGCAGCAGGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCAAAGGCCTGAGCGTCGTAg          <  1:323797/147‑1 (MQ=255)
                                                                                                                                                     aaaaaaATACCCCTTTTTCCCCCCGATTTGGTTCACACCCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCAAAGGCCTGAGCGTCGTAGccc       <  1:650396/148‑1 (MQ=255)
                                                                                                                                                     aaaaaaATACCCCTTTTTACCGCCGATTTGGTTCACTCCCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCAAAGGCCTGAGCGTCGTa           <  2:591662/144‑1 (MQ=255)
                                                                                                                                                      aaGAAATAACGCTCTTTACCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCAAAGGCCTGAGCGTCGTAGCCCCAg    >  1:460175/1‑150 (MQ=255)
                                                                                                                                                       aGAAATAACGCTCTTTACCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCAAAGGCCTGAGCGTCGTAGCCCCAGTc  >  2:409146/1‑151 (MQ=255)
                                                                                                                                                        |                                                                                                                                                         
AAGTTGTTTTTCCGGTTCAACGACAATCACTTTCCAGCTATCCACCGCATCGCCAGTCTGTAAATATTCGCGCTCCGGGCGGCCTTTCGCCAGCTTATGACCGATCGCGCGGTCCATCAACGCCCGTGTCTGCCACAAAATATTGCCAAAGAAATAACGCTCTTTACCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCAAAGGCCTGAGCGTCGTAGCCCCAGTC  >  NC_000913/907068‑907367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: