Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 907,218 | 0 | G | A | 40.4% | 56.6 / 26.7 | 52 | F355F (TTC→TTT) | ybjT | putative NAD‑dependent oxidoreductase |
Reads supporting (aligned to +/- strand): ref base G (20/11); new base A (0/21); total (20/32) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 8.91e-07 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AAGTTGTTTTTCCGGTTCAACGACAATCACTTTCCAGCTATCCACCGCATCGCCAGTCTGTAAATATTCGCGCTCCGGGCGGCCTTTCGCCAGCTTATGACCGATCGCGCGGTCCATCAACGCCCG‑TGTCTGCCACAAAATATTGCC‑AAAGAA‑ATAA‑CGCTCTTT‑A‑CCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCAAAGGCCTGAGCGTCGTAGCCCCAGTC > NC_000913/907068‑907367 | aaGTTGTTTTTCCGGTTCAACGACAATCACTTTCCAGCTATCCACCGCATCGCCAGTCTGTAAATATTCGCGCTCCGGGCGGCCTTTCGCCAGCTTATGACCGATCGCGCGGTCCATCAACGCCCG‑TGTCTGCCACAAAATATTGCC‑AAAg < 2:674258/151‑1 (MQ=255) gttTTTCCGGTTCAACGACAATCACTTTCCAGCTATCCACCGCATCGCCAGTCTGTAAATATTCGCGCTCCGGGCGGCCTTTCGCCAGCTTATGACCGATCGCGCGGTCCATCAACGCCCG‑TGTCTGCCACAAAATATTGCC‑AAAGAA‑At > 2:224642/1‑150 (MQ=255) tttttCCGGTTCAACGACAATCACTTTCCAGCTATCCACCGCATCGCCAGTCTGTAAATATTCGCGCTCCGGGCGGCCTTTCGCCAGCTTATGACCGATCGCGCGGTCCATCAACGCCCG‑TGTCTGCCACAAAATATTGCC‑AAAGAA‑ATa > 1:992827/1‑150 (MQ=255) aaCGACAATCACTTTCCAGCTATCCACCGCATCGCCAGTCTGTAAATATTCGCGCTCCGGGCGGCCTTTCGCCAGCTTATGACCGATCGCGCGGTCCATCAACGCCCG‑TGTCTGCCACAAAATATTGCC‑AAAGAA‑ATAA‑CGCTCTTT‑A‑ccg > 2:87397/1‑151 (MQ=255) aCGACAATCACTTTCCAGCTATCCACCGCATCGCCAGTCTGTAAATATTCGCGCTCCGGGCGGCCTTTCGCCAGCTTATGACCGATCGCGCGGTCCATCAACGCCCG‑TGTCTGCCACAAAATATTGCC‑AAAGAA‑ATAA‑CGCTCTTT‑A‑ccgc < 2:311338/151‑1 (MQ=255) aCAATCACTTTCCAGCTATCCACCGCATCGCCAGTCTGTAAATATTCGCGCTCCGGGCGGCCTTTCGCCAGCTTATGACCGATCGCGCGGTCCATCAACGCCCG‑TGTCTGCCACAAAATATTGCC‑AAAGAA‑ATAA‑CGCTCTTT‑A‑ccgccg > 2:723478/1‑150 (MQ=255) cAATCACTTTCCAGCTATCCACCGCATCGCCAGTCTGTAAATATTCGCGCTCCGGGCGGCCTTTCGCCAGCTTATGACCGATCGCGCGGTCCATCAACGCCCG‑TGTCTGCCACAAAATATTGCC‑AAAGAA‑ATAA‑CGCTCTTT‑A‑ccgccg < 2:62952/149‑1 (MQ=255) cACTTTCCAGCTACCCCCCGCATCGCCAGTCTGTAAATATTCGCGCTCCGGGCGGCCTTTCGCCAGCTTATGACCGATCCCGCGGTCCATCAACGCCCG‑TGTCTGCCACAAAATATTGCC‑AAAGAA‑ATAA‑CGCTCTTT‑A‑CCGCCGAttt < 1:1094115/149‑1 (MQ=255) cTTTCCAGCTATCCCCCGCATCGCCAGTCTGTAAATATTCGCGCTCCGGGCGGCCTTTCGCCAGCTTATGACCGATCGCGCGGTCCATCAACGCCCG‑TGTCTGCCACAAAATATTGCC‑AAAGAA‑ATAA‑CGCTCTTT‑A‑CCGCCGATTTGGt < 1:297225/150‑1 (MQ=255) gCTATCCACCGCATCGCCAGTCTGTAAATATTCGCGCTCCGGGCGGCCTTTCGCCAGCTTATGACCGATCGCGCGGTCCATCAACGCCCG‑TGTCTGCCACAAAATATTGCC‑AAAGAA‑ATAA‑CGCTCTTT‑A‑CCGCCGATTTGGTTCACTAcc > 2:834625/1‑151 (MQ=255) aTCCACCGCATCGCCAGTCTGTAAATATTCGCGCTCCGGGCGGCCTTTCGCCAGCTTATGACCGATCGCGCGGTCCATCAACGCCCG‑TGTCTGCCACAAAATATTGCC‑AAAGAA‑ATAA‑CGCTCTTT‑A‑CCGCCGATTTGGTTCACTACCt < 1:117561/149‑1 (MQ=255) aTCCACCGCATCGCCAGTCTGTAAATATTCGCGCTCCGGGCGGCCTTTCGCCAGCTTATGACCGATCGCGCGGTCCATCAACGCCCG‑TGTCTGCCACAAAATATTGCC‑AAAGAA‑ATAA‑CGCTCTTT‑A‑CCGCCGATTTGGTTCACTACCTGc > 1:1238932/1‑151 (MQ=255) ccACCGCATCGCCAGTCTGTAAATATTCGCGCTCCGGGCGGCCTTTCGCCAGCTTATGACCGATCGCGCGGCCCATCAACGCCCG‑TGTCTGCCACAAAATATTGCC‑AAAGAA‑ATAA‑CGCTCTTT‑A‑CCGCCGATTTGGTTCACTACCTGcc < 2:836582/150‑1 (MQ=255) cGCCAGTCTGTAAATATTCGCGCTCCGGGCGGCCTTTCGCCAGCTTATGACCGATCGCGCGGTCCATCAACGCCCG‑TGTCTGCCACAAAATATTGCC‑AAAGAA‑ATAA‑CGCTCTTT‑A‑CCGCCGATTTGGTTCACTACCTGCCATAAagcagc > 1:1241742/1‑151 (MQ=255) gTAAATATTCGCGCTCCGGGCGGCCTTTCGCCAGCTTATGACCGATCGCGCGGTCCATCAACGCCCG‑TGTCTGCCACAAAATATTGCC‑AAAGAA‑ATAA‑CGCTCTTT‑A‑CCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTgg > 1:193713/1‑150 (MQ=255) aaatttcccccccccGGGCGCCTTTTCGCCAGCTTATGCCCGACCGCCCTGCCCATCACCCCCCG‑TGTCTCCCACAAAATATGGCC‑AAAAAA‑AAAC‑CGCTTTTT‑C‑CCGCAAATTTGGTTCACTCCCTGCCATAAAGCAGCAAGGCTg < 1:913513/134‑1 (MQ=255) aaaTTTTCGCCCTCCGGGCGCCTTTTCACCACTTTACGACCGATCGCGGGTCCCACCACCGCCCG‑GTTCTGCCAAAAAATATTCCC‑AAAAAA‑AAAA‑CCCTCTTT‑A‑CCGCCGATTTGGTTCACTCCCTGCCATAAAGCAGCAAGGCTGGc < 1:482223/149‑1 (MQ=255) aaaTATTCGCCCTCCGGGCGGCCTTTCGCCAGCTTATGACCGATCGCGCGGTCCATCAACGCCCG‑TGTCTGCCACAAAATATTGCC‑AAAGAA‑ATAA‑CGCTCTTT‑C‑CCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTgg < 2:10893/148‑1 (MQ=255) cgccccccGGGCGGCCTTCCGCCACCTTATGACCGAGCGCGCGGCCCATCAACGCCCG‑TGTCTGCCACAAAATATTGCC‑AAAGAA‑ATAA‑CGCCTTTT‑C‑CCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGtttt < 2:18413/145‑1 (MQ=255) cgcCCCGGGCGCCTTTCCCCCACCTAATAACCAACCGCGCGATCAATAACCGCCTG‑TGCCCGCAACAAAATTTTCCC‑AAAAAA‑AAAA‑CGCTCTTA‑C‑CCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTa < 1:802610/150‑1 (MQ=255) cTCCGGGCGGCCTTTCGCCAGCTTATGACCGATCGCGCGGTCCATCAACGCCCG‑TGTCTGCCACAAAATATTGCC‑AAAGAA‑ATAA‑CGCTCTTT‑A‑CCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACg > 2:461368/1‑151 (MQ=255) ccGGGCGGCCTTTCGCCAGCTTATGACCGATCGCGCGGTCCATCAACGCCCG‑TGTCTGCCACAAAATATTGCC‑AAAGAA‑ATAA‑CGCTCTTT‑A‑CCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACg > 2:800845/1‑149 (MQ=255) ggcCTTTCGCCAGCTTATGACCGATCGCGCGGTCCATCAACGCCCG‑TGTCTGCCACAAAATATTGCC‑AAAGAA‑ATCA‑CGCTCTTT‑A‑CCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCACGGCTGGCGGACGTTTTAACGGTAAAccc > 1:317844/1‑151 (MQ=255) gCCAGCTTATGACCGATCGCGCGGTCCATCAACGCCCG‑TGTCTGCCACAAAATATTGCC‑AAAGAA‑ATAA‑CGCTCTTT‑A‑CCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCACGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGtt > 2:1094452/1‑151 (MQ=255) ccgctttatgccataTCGGGGGGCCCACCACCCCCGG‑TGTTTGCCAAAAAATATGCCA‑AAAAAA‑ATAA‑CGCTCTTT‑C‑CCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGttt < 2:990748/137‑1 (MQ=255) accttaggccgacccgcgcgGCCCATCACCCCCCG‑TGTCTGCCAAAAAATATCCCA‑AAAGAA‑AAAC‑CGCTTTTT‑A‑CCGCCGATTTGGTTCACTCCCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGtt < 1:474960/134‑1 (MQ=255) ttatgccggtccgccgGTCCAACCACCCCCC‑TTTTTGCCAAAAAAATTTGCC‑AAAAAA‑AAAACCCCTTTTT‑C‑CCGCCGATTTGGTTCACTCCCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGttt < 1:1104042/132‑1 (MQ=255) aggcccGATCGCGGGGCCCATCACCCCCCG‑TGTCTGCCAAAAAATATTGCA‑AAAAAA‑AAAC‑CGCTCTTT‑A‑CCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCaa < 2:1066055/145‑1 (MQ=255) gACCGATCGCGCGGTCCATCAACGCCCG‑TGTCTGCCACAAAATATTGCC‑AAAGAA‑ATAA‑CGCTCTTT‑A‑CCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAAt > 1:30235/1‑150 (MQ=255) ccGCTCGCGC‑GTCCAAAAACCCCCGTTGTCTGCCAAAAAATTTTCCA‑AAAAAA‑AAAC‑CGCTTTTT‑C‑CCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAAt < 1:1103101/148‑1 (MQ=255) ccGACCGCGCGCTCCACCACCGCCCG‑TGTCTCCCAAAAAATTTTCCA‑AAAAAA‑AAAC‑CGCTTTTT‑C‑CCGCCGTTTTGGTCCCCTCCCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAAc < 1:1134700/151‑1 (MQ=255) gaccgcgcgCTCCAACACCCCCCG‑TGTCCCCCAAAAAAT‑TTTCC‑AAAAAA‑AAAA‑CCCCTTTT‑ACCCGCCGTTTTGGTTCACTCCCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCg < 1:328414/148‑1 (MQ=255) cgcgGTCCATCAACGCCCG‑TGTCTGCCACAAAATATTGCC‑AAAGAA‑ATAA‑CGCTCTTT‑A‑CCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTc > 2:415801/1‑151 (MQ=255) gctccacaaccGCCGG‑TGTCTGCCGCAAATTATT‑AC‑AAAAAA‑AAAA‑CCCCCTTT‑ACCCCCCGATTCGGTTCCCTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCg < 2:829544/133‑1 (MQ=255) gTCCATCAACGCCCG‑TGTCTGCCACAAAATATTGCC‑AAAGAA‑ATAA‑CGCTCTTT‑A‑CCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCgg > 2:12755/1‑150 (MQ=255) acccATCACCCCGCG‑TGTCTCCCAAAAAACATTCCA‑AAAAAA‑ATAA‑CGCTTTTA‑C‑CCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCgg < 2:167772/148‑1 (MQ=255) cccacctcGCCGG‑TTTCTCTCACAAAA‑ATTTGC‑AAAGAATATAT‑CCCCTTTT‑C‑CCGCCCTTTTGGTCCACTTCCTGCCATAATGCAGCAAGCCTGTCGCACGTTTTAACGTTAATCCCCGCCTGTCTGGCAAAATAACCGTACTCCGGTCg < 1:656247/144‑1 (MQ=255) acccccgg‑tgtCCCCCAAAAAATTTTGCA‑AAAAAA‑AAAC‑CCCTTTTC‑C‑CCGCCGTTTTGGTTCACTCCCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGc < 1:268335/140‑1 (MQ=255) accGCCCG‑TTTCTCCCAAAAATTATTGCA‑AAAAAA‑AAAC‑CGCTCTTT‑C‑CCGCCGATTTGTTTCACTCCCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCa < 2:748989/147‑1 (MQ=255) accGCCCG‑TGT‑TGCCCAAAAAATTTGCCAAAAGAA‑AGAC‑CCCTTTTC‑C‑CCGCCGTTTTGGTTCACTCCCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCg < 1:442866/144‑1 (MQ=255) aCGCCCG‑TGTCTGCCACAAAATATTGCC‑AAAGAA‑ATAA‑CGCTCTTT‑A‑CCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCg > 2:469157/1‑151 (MQ=255) ccgggttCTGCCAAAAAAT‑TTGCCAAAAAAA‑ATAC‑CCCTTTTT‑C‑CCCCCTTTTTGTTTCCCTCCCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTc < 1:983356/130‑1 (MQ=255) gcccAAAAAATAGGGCA‑AAAAAA‑AAAG‑CCCTTTTC‑C‑CCGCCAATTGGGTTCACTCCCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCAAAg < 1:1228453/145‑1 (MQ=255) ccAAAAAATATTCCA‑AAAAAA‑AAAA‑CGCTTTTT‑C‑CCGCCGATTTGGTTCCCTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCAAAGGCCt < 2:453004/149‑1 (MQ=255) ccAAAAAATATGCCA‑AAAAAA‑AAAA‑CCCTTTTT‑C‑CCGCCGATTTGGTTCACTCCCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCAAAGGCCt < 2:665496/149‑1 (MQ=255) aaaaaatttGCCAAAAAAA‑ATAC‑CGCGCTTT‑C‑CCCCCGATTTGGTTCCCTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCAAAGGCCTGAGc < 1:167403/144‑1 (MQ=255) aaaaaTATTGCA‑AAAAAA‑AAAC‑CCCTTTTT‑C‑CCGCCGTTTTGGTTCACTCCCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCAAAGGCCTGAGcg < 2:1010570/150‑1 (MQ=255) aaaaatttGCC‑AAAAAA‑AAAACCCCTCTTC‑A‑CCGCCGATTTGGTTCACTCCCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCAAAGGCCTGAGcg < 1:435250/145‑1 (MQ=255) aaaTATTGCC‑AAAGAA‑ATAA‑CGCTCTTT‑A‑CCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCAAAGGCCTGAGcgtc > 2:764043/1‑151 (MQ=255) tatTGCC‑AAAGAA‑ATAA‑CGCTCTTT‑A‑CCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCAAAGGCCTGAGcgtcgt > 2:1036887/1‑150 (MQ=255) c‑AAAAAA‑ATAA‑CCGTCTTTCC‑CCGCGGATTTGGTTCTCTCCCTGCCATAAAGCAGCAGGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCAAAGGCCTGAGCGTCGTAg < 1:323797/147‑1 (MQ=255) aaaaaa‑ATAC‑CCCTTTTT‑C‑CCCCCGATTTGGTTCACACCCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCAAAGGCCTGAGCGTCGTAGccc < 1:650396/148‑1 (MQ=255) aaaaaa‑ATAC‑CCCTTTTT‑A‑CCGCCGATTTGGTTCACTCCCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCAAAGGCCTGAGCGTCGTa < 2:591662/144‑1 (MQ=255) aaGAA‑ATAA‑CGCTCTTT‑A‑CCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCAAAGGCCTGAGCGTCGTAGCCCCAg > 1:460175/1‑150 (MQ=255) aGAA‑ATAA‑CGCTCTTT‑A‑CCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCAAAGGCCTGAGCGTCGTAGCCCCAGTc > 2:409146/1‑151 (MQ=255) | AAGTTGTTTTTCCGGTTCAACGACAATCACTTTCCAGCTATCCACCGCATCGCCAGTCTGTAAATATTCGCGCTCCGGGCGGCCTTTCGCCAGCTTATGACCGATCGCGCGGTCCATCAACGCCCG‑TGTCTGCCACAAAATATTGCC‑AAAGAA‑ATAA‑CGCTCTTT‑A‑CCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCAAAGGCCTGAGCGTCGTAGCCCCAGTC > NC_000913/907068‑907367 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |