Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	993,913	0	G	C	44.4%	87.8 / 15.2	54	G44G (GGC→GGG)	ssuB	aliphatic sulfonate ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base G (23/7);  new base C (1/23);  total (24/30)
Fisher's exact test for biased strand distribution p-value = 4.12e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTT‑CAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAA  >  NC_000913/993763‑994061                                                                                                                                                        |                                                                                                                                                     ccACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTT‑CAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCgg                                                                                                                                                      >  2:585871/1‑151 (MQ=255)  cACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTT‑CAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGc                                                                                                                                                     >  1:688480/1‑151 (MQ=255)    cGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTT‑CAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGccc                                                                                                                                                   <  2:342297/151‑1 (MQ=255)     ggTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTT‑CAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGccc                                                                                                                                                   >  1:21986/1‑150 (MQ=255)      gTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTT‑CAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCccac                                                                                                                                                 <  1:612272/151‑1 (MQ=255)        aGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTT‑CAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCccacc                                                                                                                                                >  1:694606/1‑150 (MQ=255)          cAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTT‑CAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCaccacc                                                                                                                                             <  1:273414/151‑1 (MQ=255)                cgcATCCTGAAACATCATTCGCGTGTCTTCCTGAATTT‑CAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCcac                                                                                                                                        >  1:925246/1‑150 (MQ=255)                   aTCCTGAAACATCATTCGCGTGTCTTCCTGAATTT‑CAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAAc                                                                                                                                    >  2:215029/1‑151 (MQ=255)                               cttccgcgTTTTTTCCTAATTTT‑CGGCCGGGGGTGTGGTCCCCGCAAACCCACCGCCTGCGTTTGGCTTTCCCAGCCCTGCCAGCAGGCGCAGCAGGTTATTTTTCCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGc                                                                                                                            <  1:499325/143‑1 (MQ=255)                                  tCGCGTGTCTTCCTGAATTT‑CAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAat                                                                                                                      >  2:1035780/1‑150 (MQ=255)                                     tgtgtC‑TCCTTAATTTCCACCCAGGGGTGGGGTCCCCGCAACCACACCGCGGGGGTTGGGCTTTCCCCGCCCTGCCAGCAGGCGCGGCAGGTTCCTTTTGCCCCCACCGCTGCGGCCCCCCACCGCCACAAACTGACCTGCCGGaa                                                                                                                       <  2:962721/145‑1 (MQ=255)                                           ttCCAGATTTT‑CAGCCGGCGTTGGGGTCCCCTCAACCCCTGCGCCGGGGGTGGCCGTTCCCAGCCCGCCGGGCAGGCGGGGAGGGGTCTTTTTCCCCCCCCCGCCGCGGCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAAt                                                                                                              <  1:138645/149‑1 (MQ=255)                                             ccTGATTTC‑CACCCGGCGGTGTGGTCCCTGCTACCACATCCCCGGGGGTTGGCTTTCCCACCCCTGCCAGCGGGGGCAGCAGGTTACTTTTCCCCCCACCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAAt                                                                                                              <  2:896029/147‑1 (MQ=255)                                                gAATTT‑CAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTg                                                                                                       >  1:178932/1‑151 (MQ=255)                                                 atttt‑CCGCCAGCGGTGGGGTCCCCGCTAACACACCGCCGGCGTTGGGCGTTCCCAGCCCTGCCAGCGGGCGCAGCGGGGTACTTTGCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAg                                                                                                          <  1:840943/145‑1 (MQ=255)                                                   ttt‑CCGCCGGCGGTGGGGCCCCCGCAACCACCCCGCGTGCGTTTGTCTTTCCCAGCCCTCCCAGCAGGCGCAGCAGGTTACTTTTCCCCCCCCCGCTGCGGCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGt                                                                                                     <  1:640373/150‑1 (MQ=255)                                                    ttccagccaggGGGGGGGTGCCCGCAAACCCCCCGCCGGCGGTGGGCTTTTCCAGCCCGGCCAGCGGGCGCGGCAGGTTTCTTTTCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGtt                                                                                                        <  2:885795/144‑1 (MQ=255)                                                    tt‑CAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGCCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATACAGTTGGTTc                                                                                                   >  2:413346/1‑151 (MQ=255)                                                      ccacccgggggTGGGGTCCCCGAAAACACCGCGCTTGCGTTTGGCTTTCCCAGCCCCCCCAGCGGGCGCAGAGGGGTTCTTTTGCCCCCACCGCGGCGCCCCACCGCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCa                                                                                                  <  2:391495/142‑1 (MQ=255)                                                       cagccagcGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTc                                                                                                   >  2:1022473/1‑149 (MQ=255)                                                          ccgggggggtggggCCCGCAACCCCGCCGCCGGGGTTTGCCTTTCCCAGCCCGCCCAGAAGGCCAAGCGGGGTCTTTTTCCCCCCCCCGTTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTc                                                                                                   <  2:77251/138‑1 (MQ=255)                                                             gcGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGat                                                                                           >  1:134290/1‑151 (MQ=255)                                                                    ggtgCCCGCTAACACACCCCCGGGGTTTGGCTTTCCCAGCCCTCCCAGCAGGCGCAGCAGGTTATTTTTGCCCCCACCGCTGCGCCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATAttt                                                                                       <  1:959394/148‑1 (MQ=255)                                                                        cccgacacccccccGCCTGGGTTTGGCTTTCCCACCCCGCCCAGCGGGCGCAGGGGGGTTCTTTTGCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCcg                                                                                   <  2:216895/135‑1 (MQ=255)                                                                         ccGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGt                                                                                >  2:588498/1‑150 (MQ=255)                                                                          cGCAAACACGCCGCCGGGGTTTGGCTTTCCCAGCCCGGCCAGCAGGCGCAGGGGGGTTTTTTTGCCCCCCCCGCGGCGCCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTaa                                                                              <  2:780725/151‑1 (MQ=255)                                                                           gCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAAt                                                                             >  1:18124/1‑151 (MQ=255)                                                                                 acaTCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGc                                                                       >  1:1010468/1‑151 (MQ=255)                                                                                  cagccccgggggTGGGCTTTCCCAGCCCGGCCAGCAGCCGCAGCGGGGTATTTTTCCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCt                                                                      <  1:72118/141‑1 (MQ=255)                                                                                   aTCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCtt                                                                     >  1:164869/1‑151 (MQ=255)                                                                                     cGCCGGGGGTTGGCTTTCCAAGCCCCGCCGGGAGGCGCGGCAGGGTACTTTGGCCCCCCCCGCTGCGCCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGttt                                                                         <  2:462577/145‑1 (MQ=255)                                                                                     cGCCGGCGGTGGGCTTTCCCAGCCCTCCCAGCAGGCGCAGCAGGGTATTTTGCCCACCCCCGCTGCGCCCCCCCGCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCt                                                                      <  2:734322/148‑1 (MQ=255)                                                                                        cTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGc                                                                >  2:593251/1‑151 (MQ=255)                                                                                          gCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCAt                                                              >  2:782186/1‑151 (MQ=255)                                                                                            ggTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTg                                                            >  1:950167/1‑151 (MQ=255)                                                                                                 gCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGca                                                        >  1:598756/1‑150 (MQ=255)                                                                                                     ttCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCTGCGTAATGTTTGCTTACTGCATTGAGCAACAAt                                                   >  1:252971/1‑151 (MQ=255)                                                                                                       ccaccccTGCCAGCGGGCCCAGAGGGTTACTTTCCCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATgg                                                 <  1:876913/146‑1 (MQ=255)                                                                                                        caccccggCCAGCAGCCGCAGCAGGGTCTTTTTCCCACCACCGCTGCGCCCCACCGCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGc                                                <  2:342435/144‑1 (MQ=255)                                                                                                        cAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGc                                                >  1:898174/1‑151 (MQ=255)                                                                                                         acaCCTGCCAGCAGGCCCAGAGGTGTTTTTTTCCCCCCCCCGCTGCGCCCCACCCCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGcaaca                                                     <  2:793712/143‑1 (MQ=255)                                                                                                           cccTGCCAGCGGGCGCGGCGGGGTATTTTTCCCACCACGGCGGCGGCCCACGGCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGa                                                           <  2:1062702/136‑1 (MQ=255)                                                                                                            cccgCCAGCAGGCGCAGCGGGGTTTTTTTCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCg                                               <  2:694955/145‑1 (MQ=255)                                                                                                             cTGCCAGCGGGCGCAGCAGGTTTCTTTTCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTg                                             <  1:861642/149‑1 (MQ=255)                                                                                                                       gcgcAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTc                                    >  2:1020017/1‑148 (MQ=255)                                                                                                                        cgcAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGAc                                >  2:1044485/1‑151 (MQ=255)                                                                                                                           agcagGGTCCTTTCCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACg                               <  2:1063624/149‑1 (MQ=255)                                                                                                                               gggtctttttCCCCCCCCCGCTGCGCCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGa                              <  1:431622/140‑1 (MQ=255)                                                                                                                                          ccccccccccTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATAc                 <  1:627509/139‑1 (MQ=255)                                                                                                                                          ccccccccGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCg               <  1:777788/144‑1 (MQ=255)                                                                                                                                           ccccaccGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTg             <  2:191855/149‑1 (MQ=255)                                                                                                                                            ccccccGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTgg            <  1:513989/147‑1 (MQ=255)                                                                                                                                              caccGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGcc          >  2:915158/1‑151 (MQ=255)                                                                                                                                                   cTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCtt     >  2:1015359/1‑151 (MQ=255)                                                                                                                                                       ggCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCaa  >  1:691651/1‑150 (MQ=255)                                                                                                                                                        |                                                                                                                                                     CCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTT‑CAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAA  >  NC_000913/993763‑994061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑