Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,850,007 | 0 | G | A | 45.8% | 53.6 / 39.3 | 59 | P44S (CCG→TCG) | uhpB | sensory histidine kinase in two‑component regulatory sytem with UhpA |
Reads supporting (aligned to +/- strand): ref base G (26/6); new base A (0/27); total (26/33) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.32e-11 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GTCAGTAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCG‑ACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATACGGG‑CCAGTATCCGCGCGGGCATTGCAGCATTAGCCCCAGACGCAGACCAAACGGAAATAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATAAAAAAGCAGGCAATAACGGTAATTAAGCGGGAGAACAACGTCTTCATCACCAGCCATCAAACATGCG > NC_000913/3849859‑3850156 | gTCAGTAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCG‑ACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATACGGG‑CCAGTATCCGCGCGGGCATTGCAGCATTAGCCCCAGACGCAGACCAAACGGa > 2:1043799/1‑150 (MQ=255) aacctcccGATCATCATAAACGGAAAATGGGTTAACCCG‑ACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACGCCGCGCCCAGCAATACGGG‑CCAGTATCCGCGCGGGCATTGCAGCATTAGCCCCAGACGCAGACCAAACGGAAATAAc < 2:135472/144‑1 (MQ=255) aaccTACCGATCATCAATAACGGAAAATGGGTTAACCCG‑ACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATACGGG‑CCAGTATCCGCGCGGGCATTGCAGCATTAGCCCCAGACGCAGACCAAACGGAAATaa < 1:268109/146‑1 (MQ=255) aaCTACCGATCATCAATAACGGAAAATGGGTTAAACCG‑ACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATACGGG‑CCAGTATCCGCGCGGGCATTGCAGCATTAGCCCCAGACGCAGACCAAACGGAAATAAc > 1:127096/1‑149 (MQ=255) aCTACCGATCATCAATAACGGAAAATGGGTTAAACCG‑ACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATACGGG‑CCAGTATCCGCGCGGGCATTGCAGCATTAGCCCCAGACGCAGACCAAACGGAAATAACAGc > 1:229934/1‑151 (MQ=255) aCCGATCATCAATAACGGAAAATGGGTTAAACCG‑ACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATACGGG‑CCAGTATCCGCGCGGGCATTGCAGCATTAGCCCCAGACGCAGACCAAACGGAAATAACAGCa < 1:93294/149‑1 (MQ=255) ccGATCATCAATAACGGAAAATGGGTTAAACCG‑ACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATACGGG‑CCAGTATCCGCGCGGGCATTGCAGCATTAGCCCCAGACGCAGACCAAACGGAAATAACAGCACCg < 1:356871/151‑1 (MQ=255) tcatcaATAACGGAAAATGGGTTAAACCG‑ACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATACGGG‑CCAGTATCCGCGCGGGCATTGCAGCATTAGCCCCAGACGCAGACCAAACGGAAATAACAGCACCGCCat > 2:308720/1‑151 (MQ=255) tcatcaATAACGGAAAATGGGTTAAACCG‑ACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATACGGG‑CCAGTATCCGCGCGGGCATTGCAGCATTAGCCCCAGACGCAGACCAAACGGAAATAACAGCACCGCCat > 1:1158382/1‑151 (MQ=255) tcatcaATAACGGAAAATGGGTTAAACCG‑ACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATACGGG‑CCAGTATCCGCGCGGGCATTGCAGCATTAGCCCCAGACGCAGACCAAACGGAAATAACAGCACCGCCat > 2:663922/1‑151 (MQ=255) caATAACGGAAAATGGGTTAAACCG‑ACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATACGGG‑CCAGTATCCGCGCGGGCATTGCAGCATTAGCCCCAGACGCAGACCAAACGGAAATAACAGCACCGCCATATc > 2:558550/1‑150 (MQ=255) aTAACGGAAAATGGGTTAAACCG‑ACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATACGGG‑CCAGTATCCGCGCGGGCATTGCAGCATTAGCCCCAGACGCAGACCAAACGGAAATAACAGCACCGCCATATCAg > 2:832787/1‑150 (MQ=255) ggAAAATGGGTTAAACCG‑ACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATACGGG‑CCAGTATCCGCGCGGGCATTGCAGCATTAGCCCCAGACGCAGACCAAACGGAAATAACAGCACCGCCATATCAGGGCGCt > 2:714581/1‑151 (MQ=255) aaaTGGGTTAAACCG‑ACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATACGGG‑CCAGTATCCGCGCGGGCATTGCAGCATTAGCCCCAGACGCAGACCAAACGGAAATAACAGCACCGCCATATCAGGGCGCTCa > 2:238168/1‑150 (MQ=255) aaaGGGGTTAAACCGCCCCGCCGGCGTGAGCAAAAAAACCACCCCCCCCCCCGCGCCAAGCAAGGGGGC‑CAAGTCCCCGCGCGGGCATTGCAGCATTACCCCGAGACGCAGACAAAAGGAAAAAAAAAGCACCGCCATATCAGGgcgc < 2:317064/148‑1 (MQ=255) aaTGGGTTAAACCG‑ACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATACGGG‑CCAGTATCCGCGCGGGCATTGCAGCATTAGCCCCAGACGCAGACCAAACGGAAATAACAGCACCGCCATATCAGGGCGCTCAAc > 1:352769/1‑151 (MQ=255) ggttaaccccccccgCCTGCGTTAGCAATAAAACCAGCGCCCCCCCCGCCCCCAGCAATGCGGG‑CCAGTACCCGCGGGGGCATGGCAGCATTCCCCCCAGACGCAGCCAAAACGGAAATAACGGCACCGCCATATCAGGGCGCTCAAcc < 2:1014360/137‑1 (MQ=255) tAAACCG‑ACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATACGGG‑CCAGTATCCGCGCGGGCATTGCAGCATTAGCCCCAGACGCAGACCAAACGGAAATAACAGCACCGCCATATCAGGGCGCTCAACCAGATGc > 2:4055/1‑151 (MQ=255) tAAACCG‑ACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATACGGG‑CCAGTATCCGCGCGGGCATTGCAGCATTAGCCCCAGACGCAGACCAAACGGAAATAACAGCACCGCCATATCAGGGCGCTCAACCAGATGc > 1:549142/1‑151 (MQ=255) aaacccg‑ccccCCTGCGTTAGCCAGAAAACCACCACCCCCCCCGCCCCCAGAAATGCGGG‑CCAGTCCCCGCGGGGGCATGGCAGCATTACCCCCAGACGCAGACAAAACGAAAATAACAGCACCGCCATATCAGGGCGCTCAACCAGATGc < 1:814318/147‑1 (MQ=255) cg‑accgCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATACGGG‑CCAGTATCCGCGCGGGCATTGCAGCATTAGCCCCAGACGCAGACCAAACGGAAATAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCt > 1:154387/1‑151 (MQ=255) cGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATACGGG‑CCAGTATCCGCGCGGGCATTGCAGCATTAGCCCCAGACGCAGACCAAACGGAAATAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCcac > 2:1168048/1‑151 (MQ=255) aaaaCCAGCACCCCCCCCGCCCCCAGCAATACGGC‑CCAGTACCCGCGCGGGCATTGCACCATTAGCCCGAGCCGCAGACAAAAGGAAAATAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAAcc < 2:332932/150‑1 (MQ=255) aaTCAGCACCCCCCCCGCCCCCACGAATAGGGC‑CCAGTACCCGGGCGGGCATTGCAGCATTAGCCCCAGACGCAGACCAAACGAAAAAAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCAt < 1:267808/151‑1 (MQ=255) gccgcagcCCCCCCGCGCCCACAAGGCCGGA‑CCTGTTTCCGCGCGGGTATTGCAGCTTTCGCCCCAGCCGCAGTCCAAGAGGAAACACCGGAACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATg < 1:687847/147‑1 (MQ=255) ccagccccccccccccgcCCAGCAACGCGGG‑CCATTACCCGCGGGGGCATGGCAGCATTACCCCCAGACGCAGCCAAAAGGAAAAAAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGc < 2:689698/136‑1 (MQ=255) cgcccccccccccggcCCAAGAAAACGGGCCCGGTACCCGCGCGGCTATGACAGCATACCCCCCAGACGAAGCCAAAACGAAAAAAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATg < 1:632017/135‑1 (MQ=255) agcCACTCCGCGCCCAGCAATACGGG‑CCAGTATCCGCGCGGGCATTGCAGCATTAGCCCCAGACGCAGACCAAACGGAAATAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGc > 2:514814/1‑149 (MQ=255) cccccccccccccAGAAATACGGG‑CAATTCCCCGGGCGGGAATTGCACCATTACCCCAGGCCGAAAACAAAAGGAAAAAAACACCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGc < 2:121209/137‑1 (MQ=255) cgcCCAGCAATACGGG‑CCAGTATCCGCGCGGGCATTGCAGCATTAGCCCCAGACGCAGACCAAACGGAAATAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATaa > 1:194904/1‑150 (MQ=255) cagcacgccGGC‑CCAGTACCCGCGCGGGCATTGCAGCATTCCCCCCAGACGCAGACAAAACGAAAAAACCGGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATaaaaaa < 2:739070/142‑1 (MQ=255) aGCAATCCGGC‑CCAGAACCCGCGGGGGCATTGCACTATTACCCCCAGCCGCAGACAAAACGAAAAAAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATaaaaaa < 1:335392/149‑1 (MQ=255) aacaCGGG‑CCAGTACCCGCGCGGGCTTTGCAGCATTACCCCCAGACGCAGACCAAAGGAAAAAAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATAAAAAAGc < 1:137687/145‑1 (MQ=255) aaTGCGGC‑CCAGTACCCGCGGGGCCATTGCAGCATTACCCCCAGACGAAGACAAAACGAAAATACCAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATAAAAAAGCAgg < 1:528911/151‑1 (MQ=255) ggg‑CAAGTCCCCGCGCGGGTATGGCAGTATTGCCCCCAGAGCCAGCCAAAGCGAAAAAACCAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATAAAAAAGCAGGCAATa < 1:1169618/151‑1 (MQ=255) ccAGTACCCGCGCGGGAATGGCAGCATAACCCCCAGACGAAGACAAAAGGAAAAAAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATAAAAAAGCAGGCAATAAc < 1:1062013/150‑1 (MQ=255) cAGTACCCGCGGGGGCATTGCAGCATTACCCCCAGACGCAGACAAAACGAAAAAAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATAAAAAAGCAGGCAATAAc < 1:73807/149‑1 (MQ=255) aGTATCCGCGCGGGCATTGCAGCATTAGCCCCAGACGCAGACCAAACGGAAATAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATAAAAAAGCAGGCAATAACGGt > 2:294444/1‑151 (MQ=255) tctCCGGGGGGCCATCGCAGCATGACCCCCGGCCGCACACCAAACGAAAATAACAACCCCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATAAAAAAGCAGGCAATAACGGTa < 2:97146/148‑1 (MQ=255) gcgGGCATTGCAGCATAACCCCCAGACACAGACAAAACGAAAAAAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATAAAAAAGCAGGCAATAACGGTAATTAAGc < 1:557612/150‑1 (MQ=255) ggtcATGGCACCATAACCCCCAGGGGAAAACAAAGCGAAAAAAACACCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATAAAAAAGCAGGCAATAACGGTAATTAAg < 2:106000/144‑1 (MQ=255) gggCATTGCAGAATCACCCCCAGACGAAGACAAAACGAAAATAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATAAAAAAGCAGGCAATAACGGTAATTAAGc < 1:562816/148‑1 (MQ=255) ggactttGCAGCTTTACCCCCAGCCGCAGACAAAAGGAAAAAAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATAAAAAAGCAGGCAATAACGGTAAt < 1:1009569/138‑1 (MQ=255) ggCATGGCAGCATTACCCCCAGACGAAACCAAAACAAAAAAAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATAAAAAAGCAGGCAATAACGGTAATTAAGCGGga < 1:905485/151‑1 (MQ=255) aTTGCAGCATTAGCCCCAGACGCAGACCAAACGGAAATAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATAAAAAAGCAGGCAATAACGGTAATTAAGCGGgag > 1:20862/1‑149 (MQ=255) tGCAGCATGACCCCCAGCCGCAGACAAAAGGAAAAAAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATAAAAAAGCAGGCAATAACGGTAATTAAGCGGgag < 1:504122/147‑1 (MQ=255) agcaTTGCCCCCAGACGCAGACCAAACGAAAATAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATAAAAAAGCAGGCAATAACGGTAATTAAGCGGGAGaacaac < 1:80063/150‑1 (MQ=255) tcacccccAGCCGCAAACAAAAGGAAAAAAACACCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATAAAAAAGCAGGCAATAACGGTAATTAAGCGGGAGaacaa < 2:202800/141‑1 (MQ=255) taacccccAGACGAAAACAAAAGGAAAAAAACAACACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATAAAAAAGCAGGCAATAACGGTAATTAAGCGGGAGAACAACGTc < 2:140886/145‑1 (MQ=255) taacccccAGACCCAACCAAAAAGAAAATAACAGCCCCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATAAAAAAGCAGGCAATAACGGTAATTAAGCGGGAGaa < 1:556614/138‑1 (MQ=255) tAGCCCCAGACGCAGACCAAACGGAAATAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATAAAAAAGCAGGCAATAACGGTAATTAAGCGGGAGAACAACGTCtt > 1:664146/1‑150 (MQ=255) aGCCCCAGACGCAGACCAAACGGAAATAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATAAAAAAGCAGGCAATAACGGTAATTAAGCGGGAGAACAACGTCTtc > 1:793477/1‑150 (MQ=255) aGACCAAACGAAAAAAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATAGAAAAGCAGGCAATAACGGTAATTAAGCGGGAGAACAACGTCTTCATCACCAGCCAt < 1:80305/150‑1 (MQ=255) aGACAAAACGAAAAAAAGACCCCCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATAAAAAAGCAGGCAATAACGGTAATTAAGCGGGAGAACAACGTCTTCATCACCAGCCAt < 2:983842/150‑1 (MQ=255) gACCAAACGGAAATAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATAAAAAAGCAGGCAATAACGGTAATTAAGCGGGAGAACAACGTCTTCATCACCAGCCATc > 2:444334/1‑150 (MQ=255) gACCAAACGGAAATAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATAAAAAAGCAGGCAATAACGGTAATTAAGCGGGAGAACAACGTCTTCATCACCAGCCATc > 1:441589/1‑150 (MQ=255) cAAACGGAAATAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATAAAAAAGCAGGCAATAACGGTAATTAAGCGGGAGAACAACGTCTTCATCACCAGCCATCAAAc > 1:1135671/1‑151 (MQ=255) aaaCGGAAATAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATAAAAAAGCAGGCAATAACGGTAATTAAGCGGGAGAACAACGTCTTCATCACCAGCCATCAAAc > 1:167170/1‑150 (MQ=255) aCGGAAATAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATAAAAAAGCAGGCAATAACGGTAATTAAGCGGGAGAACAACGTCTTCATCACCAGCCATCAAACATg > 2:1038178/1‑151 (MQ=255) ggAAATAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATAAAAAAGCAGGCAATAACGGTAATTAAGCGGGAGAACAACGTCTTCATCACCAGCCATCAAACATgcg > 2:636758/1‑151 (MQ=255) | GTCAGTAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCG‑ACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATACGGG‑CCAGTATCCGCGCGGGCATTGCAGCATTAGCCCCAGACGCAGACCAAACGGAAATAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATAAAAAAGCAGGCAATAACGGTAATTAAGCGGGAGAACAACGTCTTCATCACCAGCCATCAAACATGCG > NC_000913/3849859‑3850156 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |