Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,821,514	T→G	I36L (ATT→CTT)	chbB ←	N,N'‑diacetylchitobiose‑specific enzyme IIB component of PTS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,821,514	0	T	G	100.0%	241.8 / NA	58	I36L (ATT→CTT)	chbB	N,N'‑diacetylchitobiose‑specific enzyme IIB component of PTS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (36/22);  total (36/22)

CATAAAGCAGCGAGTCAATTACTTCAACCGGTTTGTTGGGTAACAAACGCTGGATTTCGGGCAACATATAAGCAATCTGCGGCCCTAATAACACGACATCGGCATTCTGACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCAATAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGATACCAGTAAAGAGGTAGACATGCCCGCAGAACAAAACAGATAAATGTGTTTCTTTTCCATAAAACTGCCCTCATCGACGATTATCTGTCAGCCA  >  NC_000913/1821367‑1821653                                                                                                                                                    |                                                                                                                                            cATAAAGCAGCGAGTCAATTACTTCAACCGGTTTGTTGGGTAACAAACGCTGGATTTCGGGCAACATATAAGCAATCTGCGGCCCTAATAACACGACATCGGCATTCTGACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCaagaat                                                                                                                                          <  2:566047/151‑1 (MQ=255)                cAATTACTTCAACCGGTTTGTTGGGTAACAAACGCTGGATTTCGGGCAACATATAAGCAATCTGCGGCCCTAATAACACGACATCGGCATTCTGACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCata                                                                                                                           <  1:599386/151‑1 (MQ=255)                 aaTTACTTCAACCGGTTTGTTGGGTAACAAACGCTGGATTTCGGGCAACATATAAGCAATCTGCGGCCCTAATAACACGACATCGGCATTCTGACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCatat                                                                                                                          >  1:497692/1‑151 (MQ=255)                    tACTTCAACCGGTTTGTTGGGTAACAAACGCTGGATTTCGGGCAACATATAAGCAATCTGCGGCCCTAATAACACGACATCGGCATTCTGACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATAtttt                                                                                                                       >  2:722066/1‑151 (MQ=255)                        tCAACCGGTTTGTTGGGTAACAAACGCTGGATTTCGGGCAACATATAAGCAATCTGCGGCCCTAATAACACGACATCGGCATTCTGACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTc                                                                                                                     <  1:633919/149‑1 (MQ=255)                           aCCGGTTTGTTGGGTAACAAACGCTGGATTTCGGGCAACATATAAGCAATCTGCGGCCCTAATAACACGACATCGGCATTCTGACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCt                                                                                                                >  2:763811/1‑151 (MQ=255)                           aCCGGTTTGTTGGGTAACAAACGCTGGATTTCGGGCAACATATAAGCAATCTGCGGCCCTAATAACACGACATCGGCATTCTGACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCt                                                                                                                <  1:763836/151‑1 (MQ=255)                            ccGGTTTGTTGGGTAACAAACGCTGGATTTCGGGCAACATATAAGCAATCTGCGGCCCTAATAACACGACATCGGCATTCTGACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCtg                                                                                                               <  1:234109/151‑1 (MQ=255)                            ccGGTTTGTTGGGTAACAAACGCTGGATTTCGGGCAACATATAAGCAATCTGCGGCCCTAATAACACGACATCGGCATTCTGACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCtg                                                                                                               <  2:283642/151‑1 (MQ=255)                                   gttgGGTAACAAACGCTGGATTTCGGGCAACATATAAGCAATCTGCGGCCCTAATAACACGACATCGGCATTCTGACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTgcgcgc                                                                                                        <  2:317502/151‑1 (MQ=255)                                     tgGGTAACAAACGCTGGATTTCGGGCAACATATAAGCAATCTGCGGCCCTAATAACACGACATCGGCATTCTGACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCa                                                                                                       <  1:838067/150‑1 (MQ=255)                                       ggTAACAAACGCTGGATTTCGGGCAACATATAAGCAATCTGCGGCCCTAATAACACGACATCGGCATTCTGACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCAttt                                                                                                    >  1:256425/1‑151 (MQ=255)                                         tAACAAACGCTGGATTTCGGGCAACATATAAGCAATCTGCGGCCCTAATAACACGACATCGGCATTCTGACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCAttttt                                                                                                  >  2:626269/1‑151 (MQ=255)                                          aaCAAACGCTGGATTTCGGGCAACATATAAGCAATCTGCGGCCCTAATAACACGACATCGGCATTCTGACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTg                                                                                                 >  2:330210/1‑151 (MQ=255)                                            cAAACGCTGGATTTCGGGCAACATATAAGCAATCTGCGGCCCTAATAACACGACATCGGCATTCTGACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGAt                                                                                               >  1:194261/1‑151 (MQ=255)                                             aaaCGCTGGATTTCGGGCAACATATAAGCAATCTGCGGCCCTAATAACACGACATCGGCATTCTGACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGAt                                                                                               >  1:244156/1‑150 (MQ=255)                                                 gCTGGATTTCGGGCAACATATAAGCAATCTGCGGCCCTAATAACACGACATCGGCATTCTGACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGATAcc                                                                                            >  2:110970/1‑149 (MQ=255)                                                   tGGATTTCGGGCAACATATAAGCAATCTGCGGCCCTAATAACACGACATCGGCATTCTGACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGATACCAGt                                                                                         >  2:70380/1‑150 (MQ=255)                                                    ggATTTCGGGCAACATATAAGCAATCTGCGGCCCTAATAACACGACATCGGCATTCTGACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGATACCAGt                                                                                         <  1:811129/149‑1 (MQ=255)                                                       tttCGGGCAACATATAAGCAATCTGCGGCCCTAATAACACGACATCGGCATTCTGACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGATACCAGTaa                                                                                       >  2:415634/1‑148 (MQ=255)                                                           gggCAACATATAAGCAATCTGCGGCCCTAATAACACGACATCGGCATTCTGACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGATACCAGTAAAGAGGTa                                                                                <  1:867859/151‑1 (MQ=255)                                                           gggCAACATATAAGCAATCTGCGGCCCTAATAACACGACATCGGCATTCTGACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGATACCAGTAAAGAGGTa                                                                                <  1:48979/151‑1 (MQ=255)                                                              cAACATATAAGCAATCTGCGGCCCTAATAACACGACATCGGCATTCTGACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGATACCAGTAAAGAGGTAGAc                                                                             >  2:795055/1‑151 (MQ=255)                                                               aaCATATAAGCAATCTGCGGCCCTAATAACACGACATCGGCATTCTGACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGATACCAGTAAAGAGGTAGACa                                                                            <  1:540823/151‑1 (MQ=255)                                                               aaCATATAAGCAATCTGCGGCCCTAATAACACGACATCGGCATTCTGACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGATACCAGTAAAGAGGTAGACa                                                                            <  2:418636/151‑1 (MQ=255)                                                                 cATATAAGCAATCTGCGGCCCTAATAACACGACATCGGCATTCTGACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGATACCAGTAAAGAGGTAGACATg                                                                          >  1:214554/1‑151 (MQ=255)                                                                    ataAGCAATCTGCGGCCCTAATAACACGACATCGGCATTCTGACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGATACCAGTAAAGAGGTAGACATGccc                                                                       >  2:6266/1‑151 (MQ=255)                                                                      aaGAAATCTGCGGCCCTAATAACACGACATCGGCATTCTGACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGATACCAGTAAAGAGGTAGACATGCCCGc                                                                     >  2:456897/1‑151 (MQ=255)                                                                          aaTCTGCGGCCCTAATAACACGACATCGGCATTCTGACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGATACCAGTAAAGAGGTAGACATGCCCGcgg                                                                   <  2:1118125/149‑3 (MQ=255)                                                                          aaTCTGCGGCCCTAATAACACGACATCGGCATTCTGACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGATACCAGTAAAGAGGTAGACATGCCCGCAGa                                                                  >  1:746094/1‑150 (MQ=255)                                                                                 ggCCCTAATAACACGACATCGGCATTCTGACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGATACCAGTAAAGAGGTAGACATGCCCGCAGAACAAAAc                                                           >  1:635264/1‑150 (MQ=255)                                                                                  gCCCTAATAACACGACATCGGCATTCTGACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGATACCAGTAAAGAGGTAGACATGCCCGCAGAACAAAAc                                                           >  1:226746/1‑149 (MQ=255)                                                                                   cccTAATAACACGACATCGGCATTCTGACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGATACCAGTAAAGAGGTAGACATGCCCGCAGAACAAAACAGa                                                        <  1:839803/151‑1 (MQ=255)                                                                                    ccTAATAACACGACATCGGCATTCTGACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGATACCAGTAAAGAGGTAGACATGCCCGCAGAACAAAACAGAt                                                       <  1:728503/151‑1 (MQ=255)                                                                                        ataaCACGACATCGGCATTCTGACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGATACCAGTAAAGAGGTAGACATGCCCGCAGAACAAAACAGATAAAt                                                   >  2:259421/1‑151 (MQ=255)                                                                                          aaCACGACATCGGCATTCTGACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGATACCAGTAAAGAGGTAGACATGCCCGCAGAACAAAACAGATAAAtgt                                                 <  2:1117032/151‑1 (MQ=255)                                                                                                aCATCGGCATTCTGACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGATACCAGTAAAGAGGTAGACATGCCCGCAGAACAAAACAGATAAATGTGTTTCt                                           >  1:560437/1‑151 (MQ=255)                                                                                                 cATCGGCATTCTGACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGATACCAGTAAAGAGGTAGACATGCCCGCAGAACAAAACAGATAAATGTGTTTCtt                                          >  2:73118/1‑151 (MQ=255)                                                                                                     ggCATTCTGACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGATACCAGTAAAGAGGTAGACATGCCCGCAGAACAAAACAGATAAATGTGTTTCTTTTcc                                      >  2:321515/1‑151 (MQ=255)                                                                                                     ggCATTCTGACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGATACCAGTAAAGAGGTAGACATGCCCGCAGAACAAAACAGATAAATGTGTTTCTTTTcc                                      >  1:36130/1‑151 (MQ=255)                                                                                                       cATTCTGACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGATACCAGTAAAGAGGTAGACATGCCCGCAGAACAAAACAGATAAATGTGTTTCTTTTCCAt                                    <  2:896809/151‑1 (MQ=255)                                                                                                            tGACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGATACCAGTAAAGAGGTAGACATGCCCGCAGAACAAAACAGATAAATGTGTTTCTTTTCCATAAAAc                               >  1:1072463/1‑151 (MQ=255)                                                                                                             gACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGATACCAGTAAAGAGGTAGACATGCCCGCAGAACAAAACAGATAAATGTGTTTCTTTTCCATAAAACt                              >  2:1053890/1‑151 (MQ=255)                                                                                                             gACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGATACCAGTAAAGAGGTAGACATGCCCGCAGAACAAAACAGATAAATGTGTTTCTTTTCCATAAAACt                              >  1:925956/1‑151 (MQ=255)                                                                                                             gACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGATACCAGTAAAGAGGTAGACATGCCCGCAGAACAAAACAGATAAATGTGTTTCTTTTCCATAAAACt                              <  1:455929/151‑1 (MQ=255)                                                                                                                    ttCACCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGATACCAGTAAAGAGGTAGACATGCCCGCAGAACAAAACAGATAAATGTGTTTCTTTTCCATAAAACTGCCCTc                        >  1:678532/1‑150 (MQ=255)                                                                                                                      cACCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGATACCAGTAAAGAGGTAGACATGCCCGCAGAACAAAACAGATAAATGTGTTTCTTTTCCATAAAACTGCCCTCATc                     >  1:723538/1‑151 (MQ=255)                                                                                                                       aCCAGCCAGTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGATACCAGTAAAGAGGTAGACATGCCCGCAGAACAAAACAGATAAATGTGTTTCTTTTCCATAAAACTGCCCTCATcg                    <  1:935850/151‑1 (MQ=255)                                                                                                                         cagccagTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGATACCAGTAAAGAGGTAGACATGCCCGCAGAACAAAACAGATAAATGTGTTTCTTTTCCATAAAACTGCCCTCATcgac                  >  1:9725/1‑151 (MQ=255)                                                                                                                         cagccagTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGATACCAGTAAAGAGGTAGACATGCCCGCAGAACAAAACAGATAAATGTGTTTCTTTTCCATAAAACTGCCCTCATcgac                  >  1:958609/1‑151 (MQ=255)                                                                                                                         cagccagTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGATACCAGTAAAGAGGTAGACATGCCCGCAGAACAAAACAGATAAATGTGTTTCTTTTCCATAAAACTGCCCTCATcgac                  <  1:220739/151‑1 (MQ=255)                                                                                                                          agccagTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGATACCAGTAAAGAGGTAGACATGCCCGCAGAACAAAACAGATAAATGTGTTTCTTTTCCATAAAACTGCCCTCATcgacg                 >  2:922663/1‑151 (MQ=255)                                                                                                                              agTGTTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGATA‑CAGTAAAGAGGTAGACATGCCCGCAGAACAAAACAGATAAATGTGTTTCTTTTCCATAAAACTGCCCTCATCGACGATTAt            >  1:733844/1‑151 (MQ=255)                                                                                                                               gtgtTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGATACCAGTAAAGAGGTAGACATGCCCGCAGAACAAAACAGATAAATGTGTTTCTTTTCCATAAAACTGCCCTCATCGACGATTAt            >  2:937295/1‑151 (MQ=255)                                                                                                                               gtgtTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGATACCAGTAAAGAGGTAGACATGCCCGCAGAACAAAACAGATAAATGTGTTTCTTTTCCATAAAACTGCCCTCATCGACGATTAt            >  1:390485/1‑151 (MQ=255)                                                                                                                                tgtTTCCGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGATACCAGTAAAGAGGTAGACATGCCCGCAGAACAAAACAGATAAATGTGTTTCTTTTCCATAAAACTGCCCTCATCGACGATTATc           >  1:168531/1‑151 (MQ=255)                                                                                                                                     ccGGAAATGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGATACCAGTAAAGAGGTAGACATGCCCGCAGAACAAAACAGATAAATGTGTTTCTTTTCCATAAAACTGCCCTCATCGACGATTATCTGTc       <  1:103245/150‑1 (MQ=255)                                                                                                                                         aaaTGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGATACCAGTAAAGAGGTAGACATGCCCGCAGAACAAAACAGATAAATGTGTTTCTTTTCCATAAAACTGCCCTCATCGACGATTATCTGTCAGCCa  <  2:574540/151‑1 (MQ=255)                                                                                                                                           aTGCTTCAAGAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGATACCAGTAAAGAGGTAGACATGCCCGCAGAACAAAACAGATAAATGTGTTTCTTTTCCATAAAACTGCCCTCATCGACGATTATCTGTCAGcc   >  1:1044585/1‑148 (MQ=255)                                                                                                                                                    |                                                                                                                                            CATAAAGCAGCGAGTCAATTACTTCAACCGGTTTGTTGGGTAACAAACGCTGGATTTCGGGCAACATATAAGCAATCTGCGGCCCTAATAACACGACATCGGCATTCTGACCTTTTTCACCAGCCAGTGTTTCCGGAAATGCTTCAATAATGACCGGAACTTCATATTTTTCTGCCTGTGCGCGCATTTTTGATACCAGTAAAGAGGTAGACATGCCCGCAGAACAAAACAGATAAATGTGTTTCTTTTCCATAAAACTGCCCTCATCGACGATTATCTGTCAGCCA  >  NC_000913/1821367‑1821653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑