Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,903,203	T→G	I41S (ATC→AGC)	manY →	mannose‑specific enzyme IIC component of PTS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,903,203	0	T	G	100.0%	276.5 / NA	73	I41S (ATC→AGC)	manY	mannose‑specific enzyme IIC component of PTS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (38/35);  total (38/35)

AAGTCTTACGTAAACAGGAGAAGTACAATGGAGATTACCACTCTTCAAATTGTGCTGGTATTTATCGTAGCCTGTATCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTATCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGAAATGATCGCGCTGGGCTGGATGAACATCGGTGCTGCAGTTGCGCCTGACGCCGCTCTGGCTTCTATCATTTCTACCATTCTGGTTATCGCAGGTCATC  >  NC_000913/1903055‑1903349                                                                                                                                                     |                                                                                                                                                   aaGTCTTACGTAAACAGGAGAAGTACAATGGAGATTACCACTCTTCAAATTGTGCTGGTATTTATCGTAGCCTGTATCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCg                                                                                                                                                  <  1:1070471/151‑1 (MQ=255)  aGTCTTTCGTTAACAGGAGAAGTTCAATGGAGATTACCACTCTTCAAATTGTGCTGGTATTTATCGTAGCCTGTATCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGt                                                                                                                                                 <  2:488262/151‑1 (MQ=255)       tACGTAAACAGGAGAAGTACAATGGAGATTACCACTCTTCAAATTGTGCTGGTATTTATCGTAGCCTGTATCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCTTTCtt                                                                                                                                             <  2:651071/150‑1 (MQ=255)       tACGTAAACAGGAGAAGTACAATGGAGATTACCACTCTTCAAATTGTGCTGGTATTTATCGTAGCCTGTATCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCtt                                                                                                                                             <  2:747746/150‑1 (MQ=255)        aCGTAAACAGGAGAAGTACAATGGAGATTACCACTCTTCAAATTGTGCTGGTATTTATCGTAGCCTGTATCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTgg                                                                                                                                           <  1:820259/151‑1 (MQ=255)            aaaCAGGAGAAGTACAATGGAGATTACCACTCTTCAAATTGTGCTGGTATTTATCGTAGCCTGTATCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGa                                                                                                                                        <  2:222223/150‑1 (MQ=255)             aaCAGGAGAAGTACAATGGAGATTACCACTCTTCAAATTGTGCTGGTATTTATCGTAGCCTGTATCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGata                                                                                                                                      <  1:855463/151‑1 (MQ=255)               cAGGAGAAGTACAATGGAGATTACCACTCTTCAAATTGTGCTGGTATTTATCGTAGCCTGTATCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATg                                                                                                                                    >  1:1020509/1‑151 (MQ=255)                aGGAGAAGTACAATGGAGATTACCACTCTTCAAATTGTGCTGGTATTTATCGTAGCCTGTATCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGa                                                                                                                                   <  1:1088158/151‑1 (MQ=255)                  gagaAGTACAATGGAGATTACCACTCTTCAAATTGTGCTGGTATTTATCGTAGCCTGTATCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGaaa                                                                                                                                 <  2:960974/151‑1 (MQ=255)                       gTACAATGGAGATTACCACTCTTCAAATTGTGCTGGTATTTATCGTAGCCTGTATCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCgg                                                                                                                            >  1:743873/1‑151 (MQ=255)                       gTACAATGGAGATTACCACTCTTCAAATTGTGCTGGTATTTATCGTAGCCTGTATCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCgg                                                                                                                            >  2:679836/1‑151 (MQ=255)                           aaTGGAGATTACCACTCTTCAAATTGTGCTGGTATTTATCGTAGCCTGTATCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGtatt                                                                                                                        <  2:905464/151‑1 (MQ=255)                           aaTGGAGATTACCACTCTTCAAATTGTGCTGGTATTTATCGTAGCCTGTATCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGtatt                                                                                                                        >  2:220257/1‑151 (MQ=255)                           aaTGGAGATTACCACTCTTCAAATTGTGCTGGTATTTATCGTAGCCTGTATCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGGGGGTAGCGTTCTTGGGGATATGAAAACCGGtat                                                                                                                         >  1:961576/1‑150 (MQ=255)                            aTGGAGATTACCACTCTTCAAATTGTGCTGGTATTTATCGTAGCCTGTATCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGtatta                                                                                                                       <  2:1026922/151‑1 (MQ=255)                            aTGGAGATTACCACTCTTCAAATTGTGCTGGTATTTATCGTAGCCTGTATCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGtatt                                                                                                                        >  2:679639/1‑150 (MQ=255)                                agaTTACCACTCTTCAAATTGTGCTGGTATTTATCGTAGCCTGTATCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGtattattat                                                                                                                   >  1:204464/1‑151 (MQ=255)                                 gaTTACCACTCTTCAAATTGTGCTGGTATTTATCGTAGCCTGTATCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATc                                                                                                                  <  2:451154/151‑1 (MQ=255)                                  aTTACCACTCTTCAAATTGTGCTGGTATTTATCGTAGCCTGTATCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATc                                                                                                                  >  1:5240/1‑150 (MQ=255)                                  aTTACCACTCTTCAAATTGTGCTGGTATTTATCGTAGCCTGTATCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCg                                                                                                                 <  2:362317/151‑1 (MQ=255)                                    tACCACTCTTCAAATTGTGCTGGTATTTATCGTAGCCTGTATCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCgg                                                                                                                >  2:728559/1‑150 (MQ=255)                                             tCAAATTGTGCTGGTATTTATCGTAGCCTGTATCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCt                                                                                                       <  1:968668/150‑1 (MQ=255)                                             tCAAATTGTGCTGGTATTTATCGTAGCCTGTATCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTg                                                                                                      >  2:225449/1‑151 (MQ=255)                                              cAAATTGTGCTGGTATTTATCGTAGCCTGTATCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTg                                                                                                      <  2:45739/150‑1 (MQ=255)                                               aaaTTGTGCTGGTATTTATCGTAGCCTGTATCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTgg                                                                                                     >  2:969855/1‑150 (MQ=255)                                                aaTTGTGCTGGTATTTATCGTAGCCTGTATCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGaa                                                                                                   <  2:1062717/151‑1 (MQ=255)                                                 aTTGTGCTGGTATTTATCGTAGCCTGTATCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGaaa                                                                                                  <  1:748771/151‑1 (MQ=255)                                                 aTTGTGCTGGTATTTATCGTAGCCTGTATCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGaa                                                                                                   >  1:146639/1‑150 (MQ=255)                                                  ttGTGCTGGTATTTATCGTAGCCTGTATCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGAAAt                                                                                                 >  1:998276/1‑151 (MQ=255)                                                         ggTATTTATCGTAGCCTGTATCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACTGGTATTATTATCGGTGGTACGCTGGAAATGATcgcg                                                                                          >  1:593049/1‑151 (MQ=255)                                                             tttATCGTAGCCTGTATCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGAAATGATCGCgctgg                                                                                      >  1:886265/1‑151 (MQ=255)                                                             tttATCGTAGCCTGTATCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGAAATGATCGCgctgg                                                                                      >  1:966072/1‑151 (MQ=255)                                                              ttATCGTAGCCTGTATCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGAAATGATCGCgctggg                                                                                     >  1:333182/1‑151 (MQ=255)                                                              ttATCGTAGCCTGTATCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGAAATGATCGCgctgg                                                                                      <  2:686287/150‑1 (MQ=255)                                                                aTCGTAGCCTGTATCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGAAATGATCGCgctgggct                                                                                   >  1:1021048/1‑151 (MQ=255)                                                                aTCGTAGCCTGTATCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGAAATGATCGCgctgggct                                                                                   <  2:671766/151‑1 (MQ=255)                                                                aTCGTAGCCTGTATCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGAAATGATCGCgctgggct                                                                                   >  1:386757/1‑151 (MQ=255)                                                                aTCGTAGCCTGTATCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGAAATGATCGCgctgggct                                                                                   <  1:911889/151‑1 (MQ=255)                                                                  cGTAGCCTGTATCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGAAATGATCGCgctgggctg                                                                                  <  2:1035566/150‑1 (MQ=255)                                                                           tATCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGAAATGATCGCGCTGGGCTGGATGAACATc                                                                        >  2:690893/1‑151 (MQ=255)                                                                            aTCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGAAATGATCGCGCTGGGCTGGATGAACATc                                                                        <  1:530263/150‑1 (MQ=255)                                                                            aTCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGAAATGATCGCGCTGGGCTGGATGAACATc                                                                        >  2:547782/1‑150 (MQ=255)                                                                            aTCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGAAATGATCGCGCTGGGCTGGATGAACATCg                                                                       <  2:14851/151‑1 (MQ=255)                                                                             tCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGAAATGATCGCGCTGGGCTGGATGAACATCgg                                                                      >  1:448707/1‑151 (MQ=255)                                                                              cGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCCCCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGAAATGATCGCGCTGGGCTGGATGAACATCGGt                                                                     <  1:575773/151‑1 (MQ=255)                                                                                cAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGAAATGATCGCGCTGGGCTGGATGAACATCGGtgc                                                                   >  1:662128/1‑151 (MQ=255)                                                                                 aGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGAAATGATCGCGCTGGGCTGGATGAACATCGGtgct                                                                  <  1:601582/151‑1 (MQ=255)                                                                                 aGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGAAATGATCGCGCTGGGCTGGATGAACATCGGtgct                                                                  >  2:584303/1‑151 (MQ=255)                                                                                  ggTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGAAATGATCGCGCTGGGCTGGATGAACATCGGtgctg                                                                 <  2:1063178/151‑1 (MQ=255)                                                                                       gggATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGAAATGATCGCGCTGGGCTGGATGAACATCGGTGCTGCAGtt                                                            <  2:519440/151‑1 (MQ=255)                                                                                             aaTCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGAAATGATCGCGCTGGGCTGGATGAACATCGGTGCTGCAGTTGCGCCt                                                      <  2:192771/151‑1 (MQ=255)                                                                                             aaTCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGAAATGATCGCGCTGGGCTGGATGAACATCGGTGCTGCAGTTGCGCCt                                                      >  2:247250/1‑151 (MQ=255)                                                                                                ccTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGAAATGATCGCGCTGGGCTGGATGAACATCGGTGCTGCAGTTGCGCCTGAc                                                   <  1:75796/151‑1 (MQ=255)                                                                                                        aaTTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGAAATGATCGCGCTGGGCTGGATGAACATCGGTGCTGCAGTTGCGCCTGACGCCGctct                                           >  2:390392/1‑151 (MQ=255)                                                                                                           ttCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGAAATGATCGCGCTGGGCTGGATGAACATCGGTGCTGCAGTTGCGCCTGACGCCGCTCTgg                                         <  1:784873/150‑1 (MQ=255)                                                                                                           ttCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGAAATGATCGCGCTGGGCTGGATGAACATCGGTGCTGCAGTTGCGCCTGACGCCGCTCTGGc                                        >  2:179451/1‑151 (MQ=255)                                                                                                             cAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGAAATGATCGCGCTGGGCTGGATGAACATCGGTGCTGCAGTTGCGCCTGACGCCGCTCTGGCt                                       <  1:610313/150‑1 (MQ=255)                                                                                                              aGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGAAATGATCGCGCTGGGCTGGATGAACATCGGTGCTGCAGTTGCGCTTGACGCCGCTCTGGCTTc                                     <  2:1021133/151‑1 (MQ=255)                                                                                                                  tCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGAAATGATCGCGCTGGGCTGGATGAACATCGGTGCTGCAGTTGCGCCTGACGCCGCTCTGGCTTCTAt                                  <  2:797607/150‑1 (MQ=255)                                                                                                                   cACCGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGAAATGATCGCGCTGGGCTGGATGAACATCGGTGCTGCAGTTGCGCCTGACGCCGCTCTGGCTTCTATc                                 >  2:614621/1‑150 (MQ=255)                                                                                                                      cGTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGAAATGATCGCGCTGGGCTGGATGAACATCGGTGCTGCAGTTGCGCCTGACGCCGCTCTGGCTTCTATCAttt                             >  1:417005/1‑151 (MQ=255)                                                                                                                       gTCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGAAATGATCGCGCTGGGCTGGATGAACATCGGTGCTGCAGTTGCGCCTGACGCCGCTCTGGCTTCTATCATTTc                            <  2:382461/151‑1 (MQ=255)                                                                                                                        tCCGCTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGAAATGATCGCGCTGGGCTGGATGAACATCGGTGCTGCAGTTGCGCCTGACGCCGCTCTGGCTTCTATCATTTCt                           >  2:792242/1‑151 (MQ=255)                                                                                                                            cTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGAAATGATCGCGCTGGGCTGGATGAACATCGGTGCTGCAGTTGCGCCTGACGCCGCTCTGGCTTCTATCATTTCTAcc                        >  1:361992/1‑150 (MQ=255)                                                                                                                            cTAATCGCGTGTACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGAAATGATCGCGCTGGGCTGGATGAACATCGGTGCTGCAGTTGCGCCTGACGCCGCTCTGGCTTCTATCATTTCTAcc                        >  2:152437/1‑150 (MQ=255)                                                                                                                                    gtgtACCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGAAATGATCGCGCTGGGCTGGATGAACATCGGTGCTGCAGTTGCGCCTGACGCCGCTCTGGCTTCTATCATTTCTACCATTCTGGtt               >  1:33493/1‑151 (MQ=255)                                                                                                                                        aCCCTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGAAATGATCGCGCTGGGCTGGATGAACATCGGTGCTGCAGTTGCGCCTGACGCCGCTCTGGCTTCTATCATTTCTACCATTCTGGTTATCg           >  1:534669/1‑151 (MQ=255)                                                                                                                                          ccTGGTGGGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGAAATGATCGCGCTGGGCTGGATGAACATCGGTGCTGCAGTTGCGCCTGACGCCGCTCTGGCTTCTATCATTTCTACCATTCTGGTTATCGc          >  2:508620/1‑150 (MQ=255)                                                                                                                                              gtggGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGAAATGATCGCGCTGGGCTGGATGAACATCGGTGCTGCAGTTGCGCCTGACGCCGCTCTGGCTTCTATCATTTCTACCATTCTGGTTATCGCAGGtc     >  1:661551/1‑151 (MQ=255)                                                                                                                                              gtggGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGAAATGATCGCGCTGGGCTGGATGAACATCGGTGCTGCAGTTGCGCCTGACGCCGCTCTGGCTTCTATCATTTCTACCATTCTGGTTATCGCAGGtc     >  1:1093062/1‑151 (MQ=255)                                                                                                                                               tggGTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGAAATGATCGCGCTGGGCTGGATGAACATCGGTGCTGCAGTTGCGCCTGACGCCGCTCTGGCTTCTATCATTTCTACCATTCTGGCTATCGCAGGtc     >  2:189974/1‑150 (MQ=255)                                                                                                                                                 ggTAGCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGAAATGATCGCGCTGGGCTGGATGAACATCGGTGCTGCAGTTGCGCCTGACGCCGCTCTGGCTTCTATCATTTCTACCATTCTGGTTATCGCAGGtcatc  <  2:1037855/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   AAGTCTTACGTAAACAGGAGAAGTACAATGGAGATTACCACTCTTCAAATTGTGCTGGTATTTATCGTAGCCTGTATCGCAGGTATGGGATCAATCCTCGATGAATTTCAGTTTCACCGTCCGCTAATCGCGTGTACCCTGGTGGGTATCGTTCTTGGGGATATGAAAACCGGTATTATTATCGGTGGTACGCTGGAAATGATCGCGCTGGGCTGGATGAACATCGGTGCTGCAGTTGCGCCTGACGCCGCTCTGGCTTCTATCATTTCTACCATTCTGGTTATCGCAGGTCATC  >  NC_000913/1903055‑1903349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑