Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,343,454	T→G	Y239S (TAT→TCT)	yfaL ←	adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,343,454	0	T	G	98.5%	248.3 / ‑3.5	66	Y239S (TAT→TCT)	yfaL	adhesin
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (26/39);  total (27/39)
Fisher's exact test for biased strand distribution p-value = 4.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.60e-01

ACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGGTTGTTAT  >  NC_000913/2343306‑2343601                                                                                                                                                     |                                                                                                                                                    ccccGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCAGaa                                                                                                                                                   <  1:541935/150‑1 (MQ=255)     gccgcAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCAGAACCtt                                                                                                                                               >  1:610057/1‑151 (MQ=255)       cgcAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCAGAACCTTCg                                                                                                                                             >  2:1115373/1‑151 (MQ=255)          agagGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCAGAACCTTCGGCa                                                                                                                                          <  1:454382/151‑1 (MQ=255)             ggaaggaCCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGCCGTCACGTTATTGGTATATATCGCCCCGCCAGAACCTTCGGCAgt                                                                                                                                        <  2:375342/150‑1 (MQ=255)             ggaaggaCCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCAGAACCTTCGGCAgtg                                                                                                                                       >  1:1034581/1‑151 (MQ=255)                          ccATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAgg                                                                                                                          >  1:1042683/1‑151 (MQ=255)                           cATAGCCTGCTGCGTTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGc                                                                                                                         <  1:845847/151‑1 (MQ=255)                           cATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGc                                                                                                                         <  2:595675/151‑1 (MQ=255)                            aTAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCa                                                                                                                        <  1:835909/151‑1 (MQ=255)                                ccTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAgtgt                                                                                                                    >  2:1022665/1‑151 (MQ=255)                                   gctgcGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGtt                                                                                                                   <  2:154179/149‑1 (MQ=255)                                      gctcttttGTTCTCAACGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAGGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTAttt                                                                                                               <  1:299262/144‑1 (MQ=255)                                            ttGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATc                                                                                                         <  2:767121/150‑1 (MQ=255)                                             tGTTCTCCTCCACAAACACGGCCCCCGTCCGGCTTTGGCCGTCATAAACAAAAAAAACAATAAGATAGGGGGCCGGCACGCTATTGGTAATTTACCCCCCGGCAGAACCTTCGGCCGTGGTATTTGTAAAGGCAGTGTTATTTACTATCg                                                                                                        <  2:972619/150‑1 (MQ=255)                                             tGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCg                                                                                                        <  1:1126582/150‑1 (MQ=255)                                                  tcATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAAc                                                                                                  >  2:739731/1‑151 (MQ=255)                                                   cATCGACTAACACGCCTCCGTTCTGGCTTTAGCTGTCAACAACAGAAATATCAATAAGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATaa                                                                                                   <  1:60663/149‑1 (MQ=255)                                                   cATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGtata                                                                                                    <  1:95920/148‑1 (MQ=255)                                                    aTCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCt                                                                                                >  1:1075031/1‑151 (MQ=255)                                                    aTCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCt                                                                                                <  2:727686/151‑1 (MQ=255)                                                      cGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGa                                                                                              <  2:100767/151‑1 (MQ=255)                                                       gACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGa                                                                                              >  1:773921/1‑150 (MQ=255)                                                         cTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCCCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAgg                                                                                           >  1:119781/1‑151 (MQ=255)                                                           aaCCCGCCTCCGTTCTGCCTGTAGCTGTCATCAACACAAATATCAATAAAATAGGGAGCCGTCACGCTTTTGGTATATTTCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGAt                                                                                         <  2:31980/151‑1 (MQ=255)                                                           aaCACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAgg                                                                                           <  2:754564/149‑1 (MQ=255)                                                            acacGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGAtg                                                                                        >  1:270663/1‑151 (MQ=255)                                                              acGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGAtgct                                                                                      >  1:996794/1‑151 (MQ=255)                                                               cGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGAtgctt                                                                                     >  2:1119244/1‑151 (MQ=255)                                                                    ccGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGATGCTTGCTg                                                                                 <  2:1101986/150‑1 (MQ=255)                                                                        tCTGGCTGTAGCTGTAATCAACAGAAATATCAATAAGATAGGGGGCCGCCCCGCTATTGGTATATATCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGATGCTTGCTGTCGc                                                                             <  1:166383/150‑1 (MQ=255)                                                                           ggCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGATGCTTGCTGTCGCta                                                                           <  2:496813/149‑1 (MQ=255)                                                                           ggCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGATGCTTGCTGTCGCta                                                                           <  2:432834/149‑1 (MQ=255)                                                                            gCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGATGCTTGCTGTCGCtatt                                                                         >  1:296481/1‑150 (MQ=255)                                                                             cTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGATGCTTGCTGTCGCtatta                                                                        <  1:763918/150‑1 (MQ=255)                                                                                tAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGATGCTTGCTGTCGCtattattat                                                                    >  2:360247/1‑151 (MQ=255)                                                                                 aGCTGTCATCAACAGAAATATCAATAAGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGATGCTTGCTGTCGCTATTATTATc                                                                   >  1:444265/1‑151 (MQ=255)                                                                                    tGTCATCAACAGAAATATCAATAAGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGATGCTTGCTGTCGCTATTATTATCGGt                                                                >  1:458486/1‑151 (MQ=255)                                                                                          caACAGAAATATCAATAAGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGATGCTTGCTGTCGCTATTATTATCGGTAACAt                                                           <  1:495084/150‑1 (MQ=255)                                                                                              aGAAATATCAATAAGATAGGGATCCGTCACGCTATTGATATAGATCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGATGCTTGCTGTCGCTATTATTATCGGTAACATCGAt                                                       <  2:587244/150‑1 (MQ=255)                                                                                                 aaTATCAATAAGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGcc                                                   >  1:447987/1‑151 (MQ=255)                                                                                                    atCAATAAGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGcccc                                                 <  1:718318/150‑1 (MQ=255)                                                                                                    atCAATAAGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGcccc                                                 <  2:75232/150‑1 (MQ=255)                                                                                                     tCAATAAGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCcg                                                <  1:702823/150‑1 (MQ=255)                                                                                                             aTAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCcgccatcgcc                                        >  1:430890/1‑150 (MQ=255)                                                                                                              tAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCcgccatcgccat                                      >  1:79299/1‑151 (MQ=255)                                                                                                              tAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCcgccatcgccat                                      >  1:267876/1‑151 (MQ=255)                                                                                                                gggAGCCGTCACGCTATTGGTATATATCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCgccatcgccatc                                     >  2:1115918/1‑150 (MQ=255)                                                                                                                 ggAGCCGTCACGCTATTGGTATATATCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCAc                                   <  1:598908/151‑1 (MQ=255)                                                                                                                  gAGCCGTCACGCTATTGGTATATATCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACt                                  >  2:752384/1‑151 (MQ=255)                                                                                                                       gTCACGCTATTGGTATATATCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAAc                             >  1:1124004/1‑151 (MQ=255)                                                                                                                         cACGCTATTGGTATATATCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACtt                           <  1:325070/151‑1 (MQ=255)                                                                                                                         cACGCTATTGGTATATATCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACtt                           >  1:683104/1‑151 (MQ=255)                                                                                                                                ttGGTATATATCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATg                    >  1:12137/1‑151 (MQ=255)                                                                                                                                   gTATATATCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGtat                 <  1:786033/151‑1 (MQ=255)                                                                                                                                    tatataTCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGtat                 <  1:68574/150‑1 (MQ=255)                                                                                                                                     atatatCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTatat               <  1:670395/151‑1 (MQ=255)                                                                                                                                      tatatCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATg              >  2:488914/1‑151 (MQ=255)                                                                                                                                         atCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGcc            <  2:45868/150‑1 (MQ=255)                                                                                                                                          tCGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCt           <  1:396781/150‑1 (MQ=255)                                                                                                                                           cGCCCCGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTg          <  2:148571/150‑1 (MQ=255)                                                                                                                                               ccGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGgttgt     <  1:190509/151‑1 (MQ=255)                                                                                                                                               ccGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGgttgt     <  2:136417/151‑1 (MQ=255)                                                                                                                                                cGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGgttgtt    >  2:213373/1‑151 (MQ=255)                                                                                                                                                cGCCAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGgttgtt    <  2:132219/151‑1 (MQ=255)                                                                                                                                                  ccAGAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGGTTGTTAt  <  2:775384/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    ACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGGTTGTTAT  >  NC_000913/2343306‑2343601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑