Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,495,641 A→C intergenic (‑349/‑4) yfdY ← / → lpxP DUF2545 family putative inner membrane protein/palmitoleoyl‑acyl carrier protein (ACP)‑dependent acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,495,6410AC98.7% 315.9 / ‑3.4 79intergenic (‑349/‑4)yfdY/lpxPDUF2545 family putative inner membrane protein/palmitoleoyl‑acyl carrier protein (ACP)‑dependent acyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base C (37/41);  total (37/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.87e-01

CATTCATGCTCCTCGTGGTTATGTCCTGACTAGTCTTTACACTCTTTACAGGAACCATTGTCGTACATGATGGCCCAACCAATTGAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTAACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGCTTGGTGTACTCTGGCTTTGGGTACAGCTTCCTTATCCTGTTCTCTGCTTTCTCGGCACGCGTATTGGCGCAATGGC  >  NC_000913/2495497‑2495787
                                                                                                                                                |                                                                                                                                                  
cATTCATGCTCCTCGTGGTTATGTCCTGACTAGTCTTTACACTCTTTACAGGAACCATTGTCGTACATGATGGCCCAACCAATTGAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATg                                                                                                                                              <  1:409042/151‑1 (MQ=255)
cATTCATGCTCCTCGTGGTTATGTCCTGACTAGTCTTTACACTCTTTACAGGAACCATTGTCGTACATGATGGCCCAACCAATTGAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATg                                                                                                                                              >  1:398179/1‑151 (MQ=255)
  ttCATGCTCCTCGTGGTTATGTCCTGACTAGTCTTTACACTCTTTACAGGAACCATTGTCGTACATGATGGCCCAACCAATTGAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGtt                                                                                                                                            >  1:732329/1‑151 (MQ=255)
    cATGCTCCTCGTGGTTATGTCCTGACTAGTCTTTACACTCTTTACAGGAACCATTGTCGTACATGATGGCCCAACCAATTGAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGttt                                                                                                                                           <  1:527090/150‑1 (MQ=255)
    cATGCTCCTCGTGGTTATGTCCTGACTAGTCTTTACACTCTTTACAGGAACCATTGTCGTACATGATGGCCCAACCAATTGAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGttt                                                                                                                                           <  2:806805/150‑1 (MQ=255)
     aTGCTCCTCGTGGTTATGTCCTGACTAGTCTTTACACTCTTTACAGGAACCATTGTCGTACATGATGGCCCAACCAATTGAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTcc                                                                                                                                         <  1:968271/151‑1 (MQ=255)
          cctcGTGGTTATGTCCTGACTAGTCTTTACACTCTTTACAGGAACCATTGTCGTACATGATGGCCCAACCAATTGAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACaa                                                                                                                                     <  2:215090/150‑1 (MQ=255)
          cctcGTGGTTATGTCCTGACTAGTCTTTACACTCTTTACAGGAACCATTGTCGTACATGATGGCCCAACCAATTGAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAAt                                                                                                                                    >  1:836757/1‑151 (MQ=255)
                ggTTATGTCCTGACTAGTCTTTACACTCTTTACAGGAACCATTGTCGTACATGATGGCCCAACCAATTGAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAAt                                                                                                                              >  1:308261/1‑151 (MQ=255)
                   tATGTCCTGACTAGTCTTTACACTCTTTACAGGAACCATTGTCGTACATGATGGCCCAACCAATTGAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAAtttt                                                                                                                           >  2:1072623/1‑151 (MQ=255)
                    aTGTCCTGACTAGTCTTTACACTCTTTACAGGAACCATTGTCGTACATGATGGCCCAACCAATTGAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTc                                                                                                                          <  1:287785/151‑1 (MQ=255)
                      gTCCTGACTAGTCTTTACACTCTTTACAGGAACCATTGTCGTACATGATGGCCCAACCAATTGAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTccc                                                                                                                        >  1:852700/1‑151 (MQ=255)
                        ccTGACTAGTCTTTACACTCTTTACAGGAACCATTGTCGTACATGATGGCCCAACCAATTGAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCcgc                                                                                                                      <  2:1114684/151‑1 (MQ=255)
                         cTGACTAGTCTTTACACTCTTTACAGGAACCATTGTCGTACATGATGGCCCAACCAATTGAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCcgcg                                                                                                                     >  2:432264/1‑151 (MQ=255)
                         cTGACTAGTCTTTACACTCTTTACAGGAACCATTGTCGTACATGATGGCCCAACCAATTGAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCcgcg                                                                                                                     >  1:1103921/1‑151 (MQ=255)
                              tAGTCTTTACACTCTTTACAGGAACCATTGTCGTACATGATGGCCCAACCAATTGAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGttt                                                                                                                >  2:961694/1‑151 (MQ=255)
                               aGTCTTTACACTCTTTACAGGAACCATTGTCGTACATGATGGCCCAACCAATTGAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGtt                                                                                                                 <  2:265309/149‑1 (MQ=255)
                               aGTCTTTACACTCTTTACAGGAACCATTGTCGTACATGATGGCCCAACCAATTGAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTc                                                                                                               <  1:875661/151‑1 (MQ=255)
                               aGTCTTTACACTCTTTACAGGAACCATTGTCGTACATGATGGCCCAACCAATTGAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTc                                                                                                               <  1:46420/151‑1 (MQ=255)
                               aGTCTTTACACTCTTTACAGGAACCATTGTCGTACATGATGGCCCAACCAATTGAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTAACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTc                                                                                                               <  2:261564/151‑1 (MQ=255)
                                gTCTTTACACTCTTTACAGGAACCATTGTCGTACATGATGGCCCAACCAATTGAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCt                                                                                                              >  1:294105/1‑151 (MQ=255)
                                  cTTTACACTCTTTACAGGAACCATTGTCGTACATGATGGCCCAACCAATTGAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTa                                                                                                             >  1:765288/1‑150 (MQ=255)
                                      acacTCTTTACAGGAACCATTGTCGTACATGATGGCCCAACCAATTGAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACAt                                                                                                          <  2:593686/149‑1 (MQ=255)
                                       cacTCTTTACAGGAACCATTGTCGTACATGATGGCCCAACCAATTGAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCt                                                                                                       <  2:353796/151‑1 (MQ=255)
                                             ttACAGGAACCATTGTCGTACATGATGGCCCAACCAATTGAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGc                                                                                                    <  1:740001/148‑1 (MQ=255)
                                              caCAGGAACCATTGTCGTACATGATGGCCCAACCAATTGAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTAc                                                                                                 >  2:460784/2‑150 (MQ=255)
                                               aCAGGAACCATTGTCGTACATGATGGCCCAACCAATTGAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTAcc                                                                                                <  2:567209/150‑2 (MQ=255)
                                               aCAGGAACCATTGTCGTACATGATGGCCCAACCAATTGAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACt                                                                                                <  1:1125636/150‑1 (MQ=255)
                                                  ggAACCATTGTCGTACATGATGGCCCAACCAATTGAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCt                                                                                            >  1:835092/1‑151 (MQ=255)
                                                   gAACCATTGTCGTACATGATGGCCCAACCAATTGAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTc                                                                                           >  1:460166/1‑151 (MQ=255)
                                                   gAACCATTGTCGTACATGATGGCCCAACCAATTGAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTc                                                                                           >  1:1091261/1‑151 (MQ=255)
                                                        aTTGTCGTACATGATGGCCCAACCAATTGAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATg                                                                                      >  1:418573/1‑151 (MQ=255)
                                                           gTCGTACATGATGGCCCAACCAATTGAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGt                                                                                    <  1:135418/150‑1 (MQ=255)
                                                             cGTACATGATGGCCCAACCAATTGAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTg                                                                                 <  1:261422/151‑1 (MQ=255)
                                                                aCATGATGGCCCAACCAATTGAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGc                                                                              <  1:528444/151‑1 (MQ=255)
                                                                       ggCCCAACCAATTGAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGCTTGgtgt                                                                       <  2:771806/151‑1 (MQ=255)
                                                                        gCCCAACCAATTGAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGCTTGGTGTa                                                                      <  2:726803/151‑1 (MQ=255)
                                                                            aaccaaTTGAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGCTTGGTGTActct                                                                  <  1:530302/151‑1 (MQ=255)
                                                                               caaTTGAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGCTTGGTGTACTCTGGc                                                               >  1:380974/1‑151 (MQ=255)
                                                                                   tGAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGCTTGGTGTACTCTGGCTTTg                                                           >  1:479019/1‑151 (MQ=255)
                                                                                    gAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGCTTGGTGTACTCTGGCTTTg                                                           <  2:960097/150‑1 (MQ=255)
                                                                                      gtgtACCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGCTTGGTGTACTCTGGCTTTGGGt                                                        >  1:479115/1‑151 (MQ=255)
                                                                                          aCCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGCTTGGTGTACTCTGGCTTTGGGTACAg                                                    <  2:816136/151‑1 (MQ=255)
                                                                                          aCCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGCTTGGTGTACTCTGGCTTTGGGTACAg                                                    <  2:130516/151‑1 (MQ=255)
                                                                                            cGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGCTTGGTGTACTCTGGCTTTGGGTACAGCt                                                  >  1:1098981/1‑151 (MQ=255)
                                                                                               tGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGCTTGGTGTACTCTGGCTTTGGGTACAGCTTcc                                               >  1:469111/1‑151 (MQ=255)
                                                                                                gTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGCTTGGTGTACTCTGGCTTTGGGTACAGCTTc                                                <  1:1044761/149‑1 (MQ=255)
                                                                                                   gACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGCTTGGTGTACTCTGGCTTTGGGTACAGCTTCCTTAt                                           >  1:97526/1‑151 (MQ=255)
                                                                                                   gACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGCTTGGTGTACTCTGGCTTTGGGTACAGCTTCCTTAt                                           <  1:451118/151‑1 (MQ=255)
                                                                                                        ggAATCTTCTGCTATACAACAATTCGTATCTACAGAATGCCACTAGGTTTCGACAATGCAAATTATCACGCGAGTTTCTACCTGCTCGCTACTGGCTCCCATGGTTTGGGCTTGGAGTACTCTGGCTTTGGGTACGGCTTCCTTATCCTGt                                      <  2:656086/151‑1 (MQ=255)
                                                                                                        ggAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGCTTGGTGTACTCTGGCTTTGGGTACAGCTTCCTTATCCTGt                                      >  1:832996/1‑151 (MQ=255)
                                                                                                          aaTCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGCTTGGTGTACTCTGGCTTTGGGTACAGCTTCCTTATCCTGTtc                                    >  1:704242/1‑151 (MQ=255)
                                                                                                           aTCTTCTGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGCTTGGTGTACTCTGGCTTTGGGTACAGCTTCCTTATCCTGTtct                                   >  2:534811/1‑151 (MQ=255)
                                                                                                               tctGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGCTTGGTGTACTCTGGCTTTGGGTACAGCTTCCTTATCCTGTtct                                   <  1:261798/147‑1 (MQ=255)
                                                                                                               tctGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGCTTGGTGTACTCTGGCTTTGGGTACAGCTTCCTTATCCTGTTCTCTg                                <  2:469193/150‑1 (MQ=255)
                                                                                                               tctGCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGCTTGGTGTACTCTGGCTTTGGGTACAGCTTCCTTATCCTGTTCTCTg                                <  2:165852/150‑1 (MQ=255)
                                                                                                                  gCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGCTTGGTGTACTCTGGCTTTGGGTACAGCTTCCTTATCCTGTTCTCTGCttt                            >  1:709831/1‑151 (MQ=255)
                                                                                                                  gCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGCTTGGTGTACTCTGGCTTTGGGTACAGCTTCCTTATCCTGTTCTCTGCt                              <  2:1020818/149‑1 (MQ=255)
                                                                                                                  gCGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGCTTGGTGTACTCTGGCTTTGGGTACAGCTTCCTTATCCTGTTCTCTGCt                              <  1:961813/149‑1 (MQ=255)
                                                                                                                   cGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGCTTGGTGTACTCTGGCTTTGGGTACAGCTTCCTTATCCTGTTCTCTGCTTtc                           <  1:138241/151‑1 (MQ=255)
                                                                                                                   cGATACAACAATTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGCTTGGTGTACTCTGGCTTTGGGTACAGCTTCCTTATCCTGTTCTCTGCTTtc                           <  2:128616/151‑1 (MQ=255)
                                                                                                                        caacaaTTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGCTTGGTGTACTCTGGCTTTGGGTACAGCTTCCTTATCCTGTTCTCTGCTTTCTCGGc                      >  2:915092/1‑151 (MQ=255)
                                                                                                                        caacaaTTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGCTTGGTGTACTCTGGCTTTGGGTACAGCTTCCTTATCCTGTTCTCTGCTTTCTCGGc                      <  2:1104981/151‑1 (MQ=255)
                                                                                                                           caaTTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGCTTGGTGTACTCTGGCTTTGGGTACAGCTTCCTTATCCTGTTCTCTGCTTTCTCGGCAcg                   <  2:199544/151‑1 (MQ=255)
                                                                                                                             aTTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGCTTGGTGTACTCTGGCTTTGGGTACAGCTTCCTTATCCTGTTCTCTGCTTTCTCGGCAcgcg                 >  2:706751/1‑151 (MQ=255)
                                                                                                                             aTTCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGCTTGGTGTACTCTGGCTTTGGGTACAGCTTCCTTATCCTGTTCTCTGCTTTCTCGGCAcgc                  >  2:725190/1‑150 (MQ=255)
                                                                                                                               tCGTATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGCTTGGTGTACTCTGGCTTTGGGTACAGCTTCCTTATCCTGTTCTCTGCTTTCTCGGCACGCGt                >  1:922510/1‑150 (MQ=255)
                                                                                                                                  tATCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGCTTGGTGTACTCTGGCTTTGGGTACAGCTTCCTTATCCTGTTCTCTGCTTTCTCGGCACGCGTATTg            >  2:1114028/1‑151 (MQ=255)
                                                                                                                                   aTCTACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGCTTGGTGTACTCTGGCTTTGGGTACAGCTTCCTTATCCTGTTCTCTGCTTTCTCGGCACGCGTATTg            <  1:1072749/150‑1 (MQ=255)
                                                                                                                                      tACAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGCTTGGTGTACTCTGGCTTTGGGTACAGCTTCCTTATCCTGTTCTCTGCTTTCTCGGCACGCGTATTGgcgc        >  2:358533/1‑151 (MQ=255)
                                                                                                                                       aCAGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGCTTGGTGTACTCTGGCTTTGGGTACAGCTTCCTTATCCTGTTCTCTGCTTTCTCGGCACGCGTATTGgc          <  2:154338/148‑1 (MQ=255)
                                                                                                                                         aGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGCTTGGTGTACTCTGGCTTTGGGTACAGCTTCCTTATCCTGTTCTCTGCTTTCTCGGCACGCGTATTGGCGCAAt     >  1:377686/1‑151 (MQ=255)
                                                                                                                                         aGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGCTTGGTGTACTCTGGCTTTGGGTACAGCTTCCTTATCCTGTTCTCTGCTTTCTCGGCACGCGTATTGGCGCAAt     >  1:737282/1‑151 (MQ=255)
                                                                                                                                         aGAAGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGCTTGGTGTACTCTGGCTTTGGGTACAGCTTCCTTATCCTGTTCTCTGCTTTCTCGGCACGCGTATTGGCGCAAt     >  1:686755/1‑151 (MQ=255)
                                                                                                                                           aaGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGCTTGGTGTACTCTGGCTTTGGGTACAGCTTCCTTATCCTGTTCTCTGCTTTCTCGGCACGCGTATTGGCGCAATgg   <  1:685740/151‑1 (MQ=255)
                                                                                                                                           aaGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGCTTGGTGTACTCTGGCTTTGGGTACAGCTTCCTTATCCTGTTCTCTGCTTTCTCGGCACGCGTATTGGCGCAATgg   <  2:377549/151‑1 (MQ=255)
                                                                                                                                           aaGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGCTTGGTGTACTCTGGCTTTGGGTACAGCTTCCTTATCCTGTTCTCTGCTTTCTCGGCACGCGTATTGGCGCAATg    <  2:689144/150‑1 (MQ=255)
                                                                                                                                            aGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGCTTGGTGTACTCTGGCTTTGGGTACAGCTTCCTTATCCTGTTCTCTGCTTTCTCGGCACGCGTATTGGCGCAATGgc  >  1:893322/1‑151 (MQ=255)
                                                                                                                                            aGGTCACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGCTTGGTGTACTCTGGCTTTGGGTACAGCTTCCTTATCCTGTTCTCTGCTTTCTCGGCACGCGTATTGGCGCAATGgc  >  2:731/1‑151 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
CATTCATGCTCCTCGTGGTTATGTCCTGACTAGTCTTTACACTCTTTACAGGAACCATTGTCGTACATGATGGCCCAACCAATTGAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTAACTATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGCTTGGTGTACTCTGGCTTTGGGTACAGCTTCCTTATCCTGTTCTCTGCTTTCTCGGCACGCGTATTGGCGCAATGGC  >  NC_000913/2495497‑2495787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: