Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,511,580	A→C	L376W (TTG→TGG)	mntH ←	manganese/divalent cation transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,511,580	0	A	C	100.0%	294.7 / NA	71	L376W (TTG→TGG)	mntH	manganese/divalent cation transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (36/35);  total (36/35)

CCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGAC  >  NC_000913/2511435‑2511727                                                                                                                                                  |                                                                                                                                                    ccGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACtt                                                                                                                                                 <  1:1041267/150‑1 (MQ=255)     cAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCt                                                                                                                                              <  1:335475/149‑1 (MQ=255)      aaTAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTc                                                                                                                                           >  2:488710/1‑151 (MQ=255)        tAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCAc                                                                                                                                         >  2:869651/1‑151 (MQ=255)         aGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACt                                                                                                                                        <  2:1120849/151‑1 (MQ=255)                       cGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAATTcagc                                                                                                                           >  1:381145/1‑150 (MQ=255)                          tCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTg                                                                                                                       >  2:165221/1‑151 (MQ=255)                           cATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTg                                                                                                                       <  1:15266/150‑1 (MQ=255)                              tCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAAc                                                                                                                   >  1:143286/1‑151 (MQ=255)                                   tACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTCTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAg                                                                                                                <  2:930155/149‑1 (MQ=255)                                           cAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCg                                                                                                      >  1:37882/1‑151 (MQ=255)                                             gcgcCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGAt                                                                                                    <  2:168753/151‑1 (MQ=255)                                                 cGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCa                                                                                                <  1:1027809/151‑1 (MQ=255)                                                          caacaaCCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTaa                                                                                        >  2:5921/1‑150 (MQ=255)                                                          caacaaCCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAAc                                                                                       >  2:885582/1‑151 (MQ=255)                                                             caacCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGt                                                                                    <  2:99552/151‑1 (MQ=255)                                                              aacCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGt                                                                                    >  2:1089224/1‑150 (MQ=255)                                                              aacCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGt                                                                                    >  2:445887/1‑150 (MQ=255)                                                              aacCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGt                                                                                    >  2:650045/1‑150 (MQ=255)                                                              aacCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGt                                                                                    >  2:512115/1‑150 (MQ=255)                                                                ccAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTAcc                                                                                 <  1:520493/151‑1 (MQ=255)                                                                  aGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTg                                                                               >  2:977340/1‑151 (MQ=255)                                                                   gATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTg                                                                               >  1:405446/1‑150 (MQ=255)                                                                    atatTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGa                                                                              >  2:862491/1‑150 (MQ=255)                                                                    atatTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGAc                                                                             <  1:531132/151‑1 (MQ=255)                                                                      atTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTc                                                                           <  2:792337/151‑1 (MQ=255)                                                                        tCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCAt                                                                         >  1:252244/1‑151 (MQ=255)                                                                          aGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATa                                                                        >  1:762195/1‑150 (MQ=255)                                                                            cgcCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAAcc                                                                     >  1:228158/1‑151 (MQ=255)                                                                             gcCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAAcc                                                                     >  1:107537/1‑150 (MQ=255)                                                                             gcCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAAcc                                                                     >  2:236470/1‑150 (MQ=255)                                                                                 cGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAAt                                                                <  1:491295/151‑1 (MQ=255)                                                                                   aCCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATcc                                                              >  2:898917/1‑151 (MQ=255)                                                                                    ccAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCg                                                             <  1:121161/151‑1 (MQ=255)                                                                                    ccAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCg                                                             >  1:288646/1‑151 (MQ=255)                                                                                     cAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCgt                                                            >  1:42632/1‑151 (MQ=255)                                                                                     cAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCgt                                                            >  2:680664/1‑151 (MQ=255)                                                                                        caccacAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTc                                                         <  1:265466/151‑1 (MQ=255)                                                                                         accacAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCg                                                        >  2:1029378/1‑151 (MQ=255)                                                                                         accacAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCg                                                        >  2:697858/1‑151 (MQ=255)                                                                                            acaATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGAt                                                     >  2:1110860/1‑151 (MQ=255)                                                                                              aaTCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCt                                                   <  1:274420/151‑1 (MQ=255)                                                                                              aaTCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCt                                                   >  1:713168/1‑151 (MQ=255)                                                                                              aaTCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCt                                                   <  2:254214/151‑1 (MQ=255)                                                                                               aTCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTa                                                  <  2:505122/151‑1 (MQ=255)                                                                                                   cccAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATc                                               <  2:1040985/150‑1 (MQ=255)                                                                                                    ccAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATccc                                             <  2:705346/151‑1 (MQ=255)                                                                                                     cAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGAGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATccc                                             >  1:1125359/1‑150 (MQ=255)                                                                                                       gCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATc                                          >  1:978641/1‑151 (MQ=255)                                                                                                               gtTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAAt                                     <  1:338790/148‑1 (MQ=255)                                                                                                               gtTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATg                                    <  2:496745/149‑1 (MQ=255)                                                                                                               gtTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGAc                                  <  2:544902/151‑1 (MQ=255)                                                                                                               gtTTTACGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGAc                                  <  1:480837/151‑1 (MQ=255)                                                                                                                    aCGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATaa                             <  1:394662/151‑1 (MQ=255)                                                                                                                    aCGCGTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATa                              >  1:599389/1‑150 (MQ=255)                                                                                                                     cgcgTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATaaa                            <  1:516373/151‑1 (MQ=255)                                                                                                                      gcgTTTGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAAt                           <  1:483649/151‑1 (MQ=255)                                                                                                                          ttGCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGAc                        <  1:275558/150‑1 (MQ=255)                                                                                                                            gCTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACgg                      <  1:488777/150‑1 (MQ=255)                                                                                                                             cTGTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGc                     >  1:273591/1‑150 (MQ=255)                                                                                                                               gTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCa                    <  1:67637/149‑1 (MQ=255)                                                                                                                               gTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCa                    <  1:812847/149‑1 (MQ=255)                                                                                                                               gTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAAc                  <  2:274985/151‑1 (MQ=255)                                                                                                                               gTTCACCAGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAATGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCaa                   >  1:537387/1‑150 (MQ=255)                                                                                                                                    ccAGATCGCCCATCCACTTGCCGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGt             <  2:989204/151‑1 (MQ=255)                                                                                                                                      aGATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTg            >  2:473927/1‑150 (MQ=255)                                                                                                                                       gATCGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGAc          >  2:555814/1‑151 (MQ=255)                                                                                                                                          cGCCCATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTg        <  1:805451/150‑1 (MQ=255)                                                                                                                                            cccATCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTac     <  2:182886/151‑1 (MQ=255)                                                                                                                                               aTCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTacgac  >  2:742003/1‑151 (MQ=255)                                                                                                                                               aTCCACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTacgac  <  1:395285/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                    CCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGAC  >  NC_000913/2511435‑2511727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑