Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,067,245	T→G	intergenic (‑72/+95)	ygfI ← / ← yggE	putative DNA‑binding transcriptional regulator/oxidative stress defense protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,067,245	0	T	G	100.0%	311.1 / NA	77	intergenic (‑72/+95)	ygfI/yggE	putative DNA‑binding transcriptional regulator/oxidative stress defense protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (39/38);  total (39/38)

GCCGCCCGGGCAATATTATTGGTTTGCGCTAATAAAATGAAATTGCGCATCTTTTTCGAAATAAAAATGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGG  >  NC_000913/3067100‑3067391                                                                                                                                                  |                                                                                                                                                   gccgccCGGGCAATATTATTGGTTTGCGCTAATAAAATGAAATTGCGCATCTTTTTCGAAATAAAAATGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAagagag                                                                                                                                                  <  2:518444/148‑1 (MQ=255) gccgccCGGGCAATATTATTGGTTTGCGCTAATAAAATGAAATTGCGCATCTTTTTCGAAATAAAAATGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAagagag                                                                                                                                                  <  1:349787/148‑1 (MQ=255)   cgccCGGGCAATATTATTGGTTTGCGCTAATAAAATGAAATTGCGCATCTTTTTCGAAATAAAAATGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGAt                                                                                                                                             <  1:138786/151‑1 (MQ=255)   cgccCGGGCAATATTATTGGTTTGCGCTAATAAAATGAAATTGCGCATCTTTTTCGAAATAAAAATGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGAt                                                                                                                                             <  2:141113/151‑1 (MQ=255)      ccGGGCAATATTATTGGTTTGCGCTAATAAAATGAAATTGCGCATCTTTTTCGAAATAAAAATGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTAc                                                                                                                                          <  1:606197/151‑1 (MQ=255)      ccGGGCAATATTATTGGTTTGCGCTAATAAAATGAAATTGCGCATCTTTTTCGAAATAAAAATGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTAc                                                                                                                                          >  1:865767/1‑151 (MQ=255)       cGGGCAATATTATTAGTTTGCGCTAATAAAATGAAATTGCGCATCTTTTTCGAAATAAAAATGTCCATCCCCCACCCCCGCATCCTTGTCCGCGTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTAc                                                                                                                                          <  2:354057/150‑1 (MQ=255)        gggCAATATTATTGGTTTGCGCTAATAAAATGAAATTGCGCATCTTTTTCGAAATAAAAATGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGt                                                                                                                                        >  2:195760/1‑151 (MQ=255)           cAATATTATTGGTTTGCGCTAATAAAATGAAATTGCGCATCTTTTTCGAAATAAAAATGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCAt                                                                                                                                     <  1:326058/151‑1 (MQ=255)             atatTATTGGTTTGCGCTAATAAAATGAAATTGCGCATCTTTTTCGAAATAAAAATGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATgg                                                                                                                                   >  1:194573/1‑151 (MQ=255)               attattGGTTTGCGCTAATAAAATGAAATTGCGCATCTTTTTCGAAATAAAAATGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGtt                                                                                                                                 >  1:263847/1‑151 (MQ=255)               attattGGTTTGCGCTAATAAAATGAAATTGCGCATCTTTTTCGAAATAAAAATGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGt                                                                                                                                  <  2:522659/150‑1 (MQ=255)                 tattGGTTTGCGCTAATAAAATGAAATTGCGCATCTTTTTCGAAATAAAAATGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGttt                                                                                                                                <  1:1108935/150‑1 (MQ=255)                  attGGTTTGCGCTAATAAAATGAAATTGCGCATCTTTTTCGAAATAAAAATGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGtttt                                                                                                                               >  1:608353/1‑150 (MQ=255)                  attGGTTTGCGCTAATAAAATGAAATTGCGCATCTTTTTCGAAATAAAAATGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTtta                                                                                                                              <  1:599411/151‑1 (MQ=255)                       tttGCGCTAATAAAATGAAATTGCGCATCTTTTTCGAAATAAAAATGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTttacaga                                                                                                                          >  2:479453/1‑150 (MQ=255)                        ttGCGCTAATAAAATGAAATTGCGCATCTTTTTCGAAATAAAAATGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTttacagaa                                                                                                                         <  1:457228/150‑1 (MQ=255)                           cgcTAATAAAATGAAATTGCGCATCTTTTTCGAAATAAAAATGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTttacagaaaatt                                                                                                                     <  1:898118/151‑1 (MQ=255)                            gcTAATAAAATGAAATTGCGCATCTTTTTCGAAATAAAAATGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTttacagaaaatt                                                                                                                     >  2:901708/1‑150 (MQ=255)                            gcTAATAAAATGAAATTGCGCATCTTTTTCGAAATAAAAATGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTttacagaaaatt                                                                                                                     <  1:980452/150‑1 (MQ=255)                              taataaAATGAAATTGCGCATCTTTTTCGAAATAAAAATGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTAAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTttacagaaaattac                                                                                                                   >  1:1054420/1‑150 (MQ=255)                                ataaAATGAAATTGCGCATCTTTTTCGAAATAAAAATGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTttacagaaaattacaga                                                                                                                <  2:180218/151‑1 (MQ=255)                                ataaAATGAAATTGCGCATCTTTTTCGAAATAAAAATGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTttacagaaaattac                                                                                                                   >  1:455309/1‑148 (MQ=255)                                  aaaaTGAAATTGCGCATCTTTTTCGAAATAAAAATGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTttacagaaaattacagaa                                                                                                               >  1:230405/1‑150 (MQ=255)                                  aaaaTGAAATTGCGCATCTTTTTCGAAATAAAAATGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTttacagaaaattacagaa                                                                                                               >  2:581865/1‑150 (MQ=255)                                   aaaTGAAATTGCGCATCTTTTTCGAAATAAAAATGTCCATCCCCCTCCCCCGCATCCTTGTCCACTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTttacagaaaattacagaaa                                                                                                              >  1:522179/1‑150 (MQ=255)                                    aaTGAAATTGCGCATCTTTTTCGAAATAAAAATGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTttacagaaaattacagaaaa                                                                                                             >  1:453385/1‑150 (MQ=255)                                     aTGAAATTGCGCATCTTTTTCGAAATAAAAATGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTttacagaaaattacagaaaa                                                                                                             >  2:47663/1‑149 (MQ=255)                                       gAAATTGCGCATCTTTTTCGAAATAAAAATGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAagg                                                                                                          <  2:1052394/150‑1 (MQ=255)                                         aaTTGCGCATCTTTTTCGAAATAAAAATGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAaggagg                                                                                                       <  2:1070186/151‑1 (MQ=255)                                          aTTGCGCATCTTTTTCGAAATAAAAATGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGc                                                                                                      <  1:526328/151‑1 (MQ=255)                                              cgcATCTTTTTCGAAATAAAAATGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAata                                                                                                  <  1:1055957/151‑1 (MQ=255)                                                     ttttCGAAATAAAAATGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGTTGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGt                                                                                            >  2:909387/1‑150 (MQ=255)                                                     ttttCGAAATAAAAATGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCgg                                                                                              <  2:764629/148‑1 (MQ=255)                                                     ttttCGAAATAAAAATGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCg                                                                                               <  1:321967/147‑1 (MQ=255)                                                     ttttCGAAATAAAAATGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCg                                                                                               <  1:883354/147‑1 (MQ=255)                                                             aTAAAAATGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCAtt                                                                                   <  2:112148/151‑1 (MQ=255)                                                               aaaaaTGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCAtt                                                                                   >  2:969971/1‑149 (MQ=255)                                                               aaaaaTGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCAtt                                                                                   >  2:749471/1‑149 (MQ=255)                                                               aaaaaTGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACAGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGCGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGCAAAGGCAtt                                                                                   >  2:685553/1‑149 (MQ=255)                                                                aaaaTGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGc                                                                                >  1:583904/1‑151 (MQ=255)                                                                 aaaTGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGcc                                                                               >  2:162784/1‑151 (MQ=255)                                                                  aaTGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGccc                                                                              <  2:1105534/151‑1 (MQ=255)                                                                     gTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCcgac                                                                           <  1:840288/151‑1 (MQ=255)                                                                     gTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTGTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCcgac                                                                           <  2:37461/151‑1 (MQ=255)                                                                         aTCCCCCTCTCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAAt                                                                       <  2:605679/151‑1 (MQ=255)                                                                         aTCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAAt                                                                       <  1:196293/151‑1 (MQ=255)                                                                          tccccctcccccGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAAt                                                                       >  1:47583/1‑150 (MQ=255)                                                                           ccccctcccccGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATAc                                                                     >  1:870807/1‑151 (MQ=255)                                                                              cctcccccGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTc                                                                  >  1:234631/1‑151 (MQ=255)                                                                                  ccccGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGc                                                              <  2:738990/151‑1 (MQ=255)                                                                                    ccGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTa                                                            <  2:502489/151‑1 (MQ=255)                                                                                      gCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTAc                                                           >  2:1040942/1‑150 (MQ=255)                                                                                       cATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACaa                                                         <  2:1067748/151‑1 (MQ=255)                                                                                          ccTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAat                                                       >  1:587398/1‑150 (MQ=255)                                                                                            ttGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAAtatt                                                    >  1:1102972/1‑151 (MQ=255)                                                                                             tGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAAtatt                                                    <  1:25596/150‑1 (MQ=255)                                                                                              gTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAAtatta                                                   <  1:339050/150‑1 (MQ=255)                                                                                                 cGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATtgt                                               >  1:1038974/1‑151 (MQ=255)                                                                                                    ttAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGtgct                                            >  1:577756/1‑151 (MQ=255)                                                                                                      aaCCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGtgctgc                                          >  1:392431/1‑151 (MQ=255)                                                                                                      aaCCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGtgctg                                           <  2:78373/150‑1 (MQ=255)                                                                                                       aCCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGtgctgc                                          >  2:139267/1‑150 (MQ=255)                                                                                                         cGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAgg                                       <  1:63018/151‑1 (MQ=255)                                                                                                          gTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGgt                                      <  1:881127/151‑1 (MQ=255)                                                                                                           tttCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGgtg                                     >  1:229505/1‑151 (MQ=255)                                                                                                               agtcagCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGtttt                                 <  1:190377/151‑1 (MQ=255)                                                                                                                gtcagCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTa                                <  1:629601/151‑1 (MQ=255)                                                                                                                   agCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCg                             >  2:635032/1‑151 (MQ=255)                                                                                                                   agCCTATCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCg                             <  1:1010441/151‑1 (MQ=255)                                                                                                                        aTCCTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGgttg                        >  2:725043/1‑151 (MQ=255)                                                                                                                          ccTTGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGgttgtt                      <  2:201864/151‑1 (MQ=255)                                                                                                                            ttGATGAAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGttgttg                     >  2:290834/1‑150 (MQ=255)                                                                                                                             tgatgaAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGAt                   >  1:291795/1‑151 (MQ=255)                                                                                                                                tgaAACCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATcc                 >  2:439691/1‑150 (MQ=255)                                                                                                                                  aaaCCGCGAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCcac               >  1:887051/1‑150 (MQ=255)                                                                                                                                      cgcgAGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGtt          >  1:497940/1‑151 (MQ=255)                                                                                                                                          aGCAAAGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTa       <  2:533190/150‑1 (MQ=255)                                                                                                                                              aaGAGAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTgg  >  1:207592/1‑151 (MQ=255)                                                                                                                                                  |                                                                                                                                                   GCCGCCCGGGCAATATTATTGGTTTGCGCTAATAAAATGAAATTGCGCATCTTTTTCGAAATAAAAATGTCCATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGG  >  NC_000913/3067100‑3067391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑