Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,378,200 T→G E3A (GAA→GCA)  rpsI ← 30S ribosomal subunit protein S9

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,378,2000TG98.5% 262.4 / NA 67E3A (GAA→GCA) rpsI30S ribosomal subunit protein S9
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (35/31);  minor base C (1/0);  total (36/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACGTTGGTTGATTACGATTTTACCGTTGCCCGGTTTGATGAAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTTCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTT  >  NC_000913/3378054‑3378347
                                                                                                                                                  |                                                                                                                                                   
gCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACGTTGGTTGATTACGATTTTACCGTTGCCCGGTTTGATGAAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGc                                                                                                                                                 <  2:93279/151‑1 (MQ=255)
   gCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACGTTGGTTGATTACGATTTTACCGTTGCCCGGTTTGACGAAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCa                                                                                                                                               <  1:529749/150‑1 (MQ=255)
     tGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACGTTGGTTGATTACGATTTTACCGTTGCCCGGTTTGATGAAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTg                                                                                                                                            >  2:49676/1‑151 (MQ=255)
     tGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACGTTGGTTGATTACGATTTTACCGTTGCCCGGTTTGATGAAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTg                                                                                                                                            >  2:217451/1‑151 (MQ=255)
      gACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACGTTGGTTGATTACGATTTTACCGTTGCCCGGTTTGATGAAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTg                                                                                                                                            <  1:480208/150‑1 (MQ=255)
      gACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACGTTGGTTGATTACGATTTTACCGTTGCCCGGTTTGATGAAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTg                                                                                                                                            <  1:516293/150‑1 (MQ=255)
         gAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACGTTGGTTGATTACGATTTTACCGTTGCCCGGTTTGATGAAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCta                                                                                                                                        <  2:835724/151‑1 (MQ=255)
          aaCTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACGTTGGTTGATTACGATTTTACCGTTGCCCGGTTTGATGAAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCtat                                                                                                                                       >  2:1076425/1‑151 (MQ=255)
           aCTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACGTTGGTTGATTACGATTTTACCGTTGCCCGGTTTGATGAAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCtata                                                                                                                                      <  1:69382/151‑1 (MQ=255)
                 aTGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACGTTGGTTGATTACGATTTTACCGTTGCCCGGTTTGATGAAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCg                                                                                                                                >  1:328861/1‑151 (MQ=255)
                       gCAGTTTCACGACCGAAGTACTGTTCCAGAGAACGTTGGTTGATTACGATTTTACCGTTGCCCGGTTTGATGAAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGAtt                                                                                                                             >  1:649067/1‑148 (MQ=255)
                       gCAGTTTCACGACCGAAGTACTGTTCCAGAGAACGTTGGTTGATTACGATTTTACCGTTGCCCGGTTTGATGAAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGa                                                                                                                          <  2:783295/151‑1 (MQ=255)
                       gCAGTTTCACGACCGAAGTACTGTTCCAGAGAACGTTGGTTGATTACGATTTTACCGTTGCCCGGTTTGATGAAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGa                                                                                                                          <  2:818479/151‑1 (MQ=255)
                           tttCACGACCGAAGTACTGTTCCAGAGAACGTTGGTTGATTACGATTTTACCGTTGCCCGGTTTGATGAAAACGCGAGCTGCGGAACTTTTGCGGCTCCCCTTGCCTTCCCCTTTCTCTCCCGCAATTGCCTATAATCCCGATTAGATGTc                                                                                                                      >  2:1056867/1‑151 (MQ=255)
                            ttCACGACCGAAGTACTGTTCCAGAGAACGTTGGTTGATTACGATTTTACCGTTGCCCGGTTTGATGAAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTc                                                                                                                      <  1:512567/150‑1 (MQ=255)
                              cACGACCGAAGTACTGTTCCAGAGAACGTTGGTTGATTACGATTTTACCGTTGCCCGGTTTGATGAAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAg                                                                                                                   <  2:832397/151‑1 (MQ=255)
                               aCGACCGAAGTACTGTTCCAGAGAACGTTGGTTGATTACGATTTTACCGTTGCCCGGTTTGATGAAAACGAGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCa                                                                                                                     >  2:410375/1‑148 (MQ=255)
                                    cGAAGTACTGTTCCAGAGAACGTTGGTTGATTACGATTTTACCGTTGCCCGGTTTGATGAAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTg                                                                                                             <  1:154093/151‑1 (MQ=255)
                                    cGAAGTACTGTTCCAGAGAACGTTGGTTGATTACGATTTTACCGTTGCCCGGTTTGATGAAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTg                                                                                                             >  1:750201/1‑151 (MQ=255)
                                       aGTACTGTTCCAGAGAGCGTTGGTTGATTACGCTTTTACCGTTGCCCGGTTTGATGAAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGc                                                                                                            >  2:197175/1‑149 (MQ=255)
                                              ttCCAGAGAACGTTGGTTGATTACGATTTTACCGTTGCCCGGTTTGATGAAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCtgt                                                                                                   >  1:936112/1‑151 (MQ=255)
                                               tCCAGAGAACGTTGGTTGATTACGATTTTACCGTTGCCCGGTTTGATGAAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCtgt                                                                                                   <  1:1118559/150‑1 (MQ=255)
                                                   gagaACGTTGGTTGATTACGATTTTACCGTTGCCCGGTTTGATGAAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCcgc                                                                                              >  2:593420/1‑151 (MQ=255)
                                                         gttggttgATTACGATTTTGCCGTTGCCCGGTTTGATGAAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGt                                                                                         <  1:369809/150‑1 (MQ=255)
                                                          ttggttgATTACGATTTTACCGTTGCCCGGTTTGATGAAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTtg                                                                                       >  2:539329/1‑151 (MQ=255)
                                                           tggttgATTACGATTTTACCGTTGCCCGGTTTGATGAAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGt                                                                                            >  1:67773/1‑145 (MQ=255)
                                                           tggttgATTACGATTTTACCGTTGCCCGGTTTGATGAAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTtgt                                                                                      >  2:605565/1‑151 (MQ=255)
                                                           tggttgATTACGATTTTACCGTTGCCCGGTTTGATGAAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTtgt                                                                                      >  1:434612/1‑151 (MQ=255)
                                                            ggttgATTACGATTTTACCGTTGCCCGGTTTGATGAAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTtgtg                                                                                     <  2:247356/151‑1 (MQ=255)
                                                               tgATTACGATTTTACCGTTGCCCGGTTTGATGAAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCt                                                                                   <  1:305230/150‑1 (MQ=255)
                                                                   tACGATTTTACCGTTGCCCGGTTTGATGAAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGtt                                                                               <  1:290345/150‑1 (MQ=255)
                                                                   tACGATTTTACCGTTGCCCGGTTTGATGAAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGtt                                                                               >  2:463726/1‑150 (MQ=255)
                                                                   tACGATTTTACCGTTGCCCGGTTTGATGAAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGtt                                                                               <  1:576715/150‑1 (MQ=255)
                                                                               gTTGCCCGGTTTGATGAAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACt                                                                  >  1:8468/1‑151 (MQ=255)
                                                                                   cccGGTTTGATGAAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCa                                                              <  1:445473/151‑1 (MQ=255)
                                                                                      ggTTTGATGAAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGtt                                                           <  1:373652/151‑1 (MQ=255)
                                                                                      ggTTTGATGAAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGtt                                                           >  1:956690/1‑151 (MQ=255)
                                                                                         ttGATGAAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTa                                                         >  1:690817/1‑150 (MQ=255)
                                                                                         ttGATGAAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTa                                                         <  2:436854/150‑1 (MQ=255)
                                                                                          tgatgaAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTAc                                                        <  1:169975/150‑1 (MQ=255)
                                                                                          tgatgaAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACg                                                       >  2:795995/1‑151 (MQ=255)
                                                                                          tgatgaAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACg                                                       >  1:211362/1‑151 (MQ=255)
                                                                                           gatgaAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACgg                                                      >  2:382569/1‑151 (MQ=255)
                                                                                            atgaAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGa                                                     <  1:1126899/151‑1 (MQ=255)
                                                                                             tgaAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGa                                                     >  1:561409/1‑150 (MQ=255)
                                                                                              gaAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAAc                                                   <  2:38949/151‑1 (MQ=255)
                                                                                               aaaaCGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAAc                                                   >  2:192079/1‑150 (MQ=255)
                                                                                               aaaaCGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAAc                                                   >  1:32167/1‑150 (MQ=255)
                                                                                                aaaCGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACAt                                                 >  2:154135/1‑151 (MQ=255)
                                                                                                aaaCGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACAt                                                 <  2:379804/151‑1 (MQ=255)
                                                                                                     cgAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCAc                                            >  1:5012/1‑151 (MQ=255)
                                                                                                       aGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACg                                           >  2:49582/1‑150 (MQ=255)
                                                                                                           gCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGAcc                                        <  2:975352/149‑1 (MQ=255)
                                                                                                            cGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAg                                     <  2:561331/151‑1 (MQ=255)
                                                                                                             ggAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCACTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGc                                    <  1:377208/151‑1 (MQ=255)
                                                                                                                  ttttGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGcc                               >  2:628736/1‑151 (MQ=255)
                                                                                                                     tGCGGCGACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCttt                            >  1:902269/1‑151 (MQ=255)
                                                                                                                       cggcgACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTg                          <  2:439011/151‑1 (MQ=255)
                                                                                                                        ggcgACCAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTgg                         >  2:22481/1‑151 (MQ=255)
                                                                                                                              cAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACAt                   <  2:587580/151‑1 (MQ=255)
                                                                                                                              cAGTGCCGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACAt                   >  1:86247/1‑151 (MQ=255)
                                                                                                                                   ccGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCtt              >  1:1004357/1‑151 (MQ=255)
                                                                                                                                    cGTAGTATTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCttt             <  2:210665/151‑1 (MQ=255)
                                                                                                                                      tagtaTTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTa            >  1:1066292/1‑150 (MQ=255)
                                                                                                                                          aTTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACcgc       >  2:730655/1‑151 (MQ=255)
                                                                                                                                          aTTGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACcgc       <  2:935994/151‑1 (MQ=255)
                                                                                                                                           ttGATTTGCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACcgcg      >  1:637129/1‑151 (MQ=255)
                                                                                                                                               tttgCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGAttt  >  1:893883/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACGTTGGTTGATTACGATTTTACCGTTGCCCGGTTTGATGAAAACGCGAGCTGCGGAACTTTTGCGGCGACCAGTGCCGTAGTATTGATTTTCAGCCATTGCCTATAATCCCGATTAGATGTCAAGAACTTGCGGTTGCTGTGCCGCGTGGTTGTGCTCGTTACCCGCGTAAACTTTCAGTTTACGGAACATAGCACGACCCAGCGGGCCTTTTGGCAACATGCCTTTAACCGCGATTT  >  NC_000913/3378054‑3378347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑