Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,927,462	A→C	E103A (GAA→GCA)	asnA →	asparagine synthetase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,927,462	0	A	C	100.0%	298.6 / NA	73	E103A (GAA→GCA)	asnA	asparagine synthetase A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (35/38);  total (35/38)

GCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTT  >  NC_000913/3927319‑3927607                                                                                                                                                |                                                                                                                                                  gCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCg                                                                                                                                             <  2:945975/150‑1 (MQ=255) gCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGt                                                                                                                                            >  2:454505/1‑151 (MQ=255)               aaGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGc                                                                                                                               >  2:176206/1‑150 (MQ=255)                 gCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCAc                                                                                                                             >  1:105818/1‑150 (MQ=255)                       ccTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCt                                                                                                                      <  1:511126/151‑1 (MQ=255)                          gATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTa                                                                                                                     <  2:273044/149‑1 (MQ=255)                          gATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATg                                                                                                                   >  2:973573/1‑151 (MQ=255)                               ccAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGAc                                                                                                              >  2:67421/1‑151 (MQ=255)                                 aGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCa                                                                                                            <  1:304745/151‑1 (MQ=255)                                     cGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTgg                                                                                                        <  1:341795/151‑1 (MQ=255)                                      gAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTggg                                                                                                       <  1:777329/151‑1 (MQ=255)                                      gAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTggg                                                                                                       >  1:129719/1‑151 (MQ=255)                                           ggTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTg                                                                                                   <  2:1040902/150‑1 (MQ=255)                                            gTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTggg                                                                                                 >  1:388640/1‑151 (MQ=255)                                             ttcattcaCTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTggg                                                                                                 >  2:433049/1‑150 (MQ=255)                                              tcattcaCTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGa                                                                                                <  1:69880/150‑1 (MQ=255)                                               cattcaCTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAAc                                                                                              >  2:51846/1‑151 (MQ=255)                                                 ttcaCTGGCGAAGTGGAAACGTCAGCCCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGaa                                                                                               <  2:833019/148‑1 (MQ=255)                                                       ggCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCGGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATg                                                                                      <  1:8163/151‑1 (MQ=255)                                                       ggCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTaa                                                                                        <  2:434560/149‑1 (MQ=255)                                                       ggCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATg                                                                                      <  1:995885/151‑1 (MQ=255)                                                       ggCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATg                                                                                      >  2:163157/1‑151 (MQ=255)                                                         cGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCG‑AGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGc                                                                                   <  1:962451/151‑1 (MQ=255)                                                          gAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGc                                                                                   >  2:585109/1‑151 (MQ=255)                                                          gAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGc                                                                                   >  1:570931/1‑151 (MQ=255)                                                          gAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGc                                                                                   <  2:684446/151‑1 (MQ=255)                                                           aaGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCg                                                                                  >  2:843235/1‑151 (MQ=255)                                                           aaGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCg                                                                                  >  2:560858/1‑151 (MQ=255)                                                           aaGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCg                                                                                  <  2:539824/151‑1 (MQ=255)                                                             gTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGAc                                                                                <  1:454571/151‑1 (MQ=255)                                                               ggAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACg                                                                               <  2:638552/150‑1 (MQ=255)                                                                   aCGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAg                                                                          <  1:93011/151‑1 (MQ=255)                                                                    cGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGc                                                                         <  1:883818/151‑1 (MQ=255)                                                                      tCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCg                                                                        >  2:411995/1‑150 (MQ=255)                                                                       cAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTc                                                                      <  2:894099/151‑1 (MQ=255)                                                                            cTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATtc                                                                 >  2:398579/1‑151 (MQ=255)                                                                                  gCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGAct                                                           <  2:99570/151‑1 (MQ=255)                                                                                    aaCACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGActct                                                         >  2:256617/1‑151 (MQ=255)                                                                                    aaCACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGActct                                                         >  1:982183/1‑151 (MQ=255)                                                                                     acacGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTg                                                        >  2:117237/1‑151 (MQ=255)                                                                                      cacGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGActct                                                         >  2:942392/1‑149 (MQ=255)                                                                                      cacGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGa                                                       <  1:333284/151‑1 (MQ=255)                                                                                         gACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGaaaa                                                    <  1:388275/151‑1 (MQ=255)                                                                                         gACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGaaa                                                     >  1:350540/1‑150 (MQ=255)                                                                                             tCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGc                                                  <  2:867770/149‑1 (MQ=255)                                                                                             tCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCa                                                 <  2:808177/150‑1 (MQ=255)                                                                                               aGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACgg                                              <  2:66337/151‑1 (MQ=255)                                                                                                gcgcGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGt                                             <  2:85540/151‑1 (MQ=255)                                                                                                    gggCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTagag                                         <  2:663566/151‑1 (MQ=255)                                                                                                      gCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGc                                       <  1:715358/151‑1 (MQ=255)                                                                                                      gCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGc                                       >  1:1118307/1‑151 (MQ=255)                                                                                                        gAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCg                                      >  2:1000527/1‑150 (MQ=255)                                                                                                        gAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCg                                      >  1:240302/1‑150 (MQ=255)                                                                                                          aGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATc                                   >  2:635824/1‑151 (MQ=255)                                                                                                          aGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATc                                   >  2:252733/1‑151 (MQ=255)                                                                                                           gggCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCt                                  <  1:973694/151‑1 (MQ=255)                                                                                                            ggCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTg                                 <  1:748350/151‑1 (MQ=255)                                                                                                             gCTGTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTgg                                >  1:368841/1‑151 (MQ=255)                                                                                                                gTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTggg                               <  2:379738/149‑1 (MQ=255)                                                                                                                gTACACGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGc                              <  2:656844/150‑1 (MQ=255)                                                                                                                     cGCACATGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAAt                        <  2:87875/151‑1 (MQ=255)                                                                                                                         caTGAAAGCCCTTCGCCGCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAAtt                       <  2:163324/148‑1 (MQ=255)                                                                                                                         caTGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTaa                     >  2:704404/1‑150 (MQ=255)                                                                                                                         caTGAAAGCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTaa                     >  1:477480/1‑150 (MQ=255)                                                                                                                                gCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCg             >  2:412073/1‑151 (MQ=255)                                                                                                                                gCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCg             <  2:145925/151‑1 (MQ=255)                                                                                                                                gCCCTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCg             >  2:1113640/1‑151 (MQ=255)                                                                                                                                  ccTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGaa           <  1:825099/151‑1 (MQ=255)                                                                                                                                   cTTCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGa            >  1:850612/1‑149 (MQ=255)                                                                                                                                     tCGCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCt        >  2:214019/1‑151 (MQ=255)                                                                                                                                       gCCCCGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTg       >  1:343650/1‑150 (MQ=255)                                                                                                                                         cccGATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCgg    <  1:1071284/151‑1 (MQ=255)                                                                                                                                            gATGCAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGtt  >  2:375150/1‑150 (MQ=255)                                                                                                                                                |                                                                                                                                                  GCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGTGAGCGTCAATTCTCGACTCTGAAAAGCACGGTAGAGGCGATCTGGGCGGGAATTAAAGCAACCGAAGCTGCGGTT  >  NC_000913/3927319‑3927607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑