Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	140,169	T→G	T353P (ACC→CCC)	gcd ←	glucose dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	140,169	0	T	G	97.2%	273.3 / ‑0.4	72	T353P (ACC→CCC)	gcd	glucose dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base G (39/31);  total (40/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01

TGGCGAGTTAAAGGTAAAGGTGTGTTCGTCAGACGGGATTGCGTTCGGATCTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGTAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCATACAGACCCGGTTTGGTGTCTGGCATATTGCTTTGCAGATTGAGCACGCCTTTATTGGCGAAGGTTTCGCACAGTTTGCCGTTTTC  >  NC_000913/140023‑140313                                                                                                                                                   |                                                                                                                                                 tGGCGAGTTAAAGGTAAAGGTGTGTTCGTCAGACGGGATTGCGTTCGGATCTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACt                                                                                                                                              >  2:982754/1‑151 (MQ=255)  ggCGAGTTAAAGGTAAAGGTGTGTTCGTCAGACGGGATTGCGTTCGGATCTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACt                                                                                                                                              <  1:840268/150‑1 (MQ=255)  ggCGAGTTAAAGGTAAAGGTGTGTTCGTCAGACGGGATTGCGTTCGGATCTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACt                                                                                                                                              <  2:235402/150‑1 (MQ=255)  ggCGAGTTAAAGGTAAAGGTGTGTTCGTCAGACGGGATTGCGTTCGGATCTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTg                                                                                                                                             >  1:125929/1‑151 (MQ=255)     gAGTTAAAGGTAAAGGTGTGTTCGTCAGACGGGATTGCGTTCGGATCTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAAc                                                                                                                                          >  2:875384/1‑151 (MQ=255)         taaaggtaaaggTGTGTTCGTCAGACGGGATTGCGTTCGGATCTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGc                                                                                                                                      >  2:419224/1‑151 (MQ=255)          aaaggtaaaggtGTGTTCGTCAGACGGGATTGCGTTCGGATCTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGcc                                                                                                                                     >  2:900789/1‑151 (MQ=255)          aaaggtaaaggtGTGTTCGTCAGACGGGATTGCGTTCGGATCTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGcc                                                                                                                                     <  1:6156/151‑1 (MQ=255)           aaggtaaaggtGTGTTCGTCAGACGGGATTGCGTTCGGATCTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCa                                                                                                                                    <  1:1063953/151‑1 (MQ=255)            aggtaaaggtGTGTTCGTCAGACGGGATTGCGTTCGGATCTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCAt                                                                                                                                   >  2:495284/1‑151 (MQ=255)            aggtaaaggtGTGTTCGTCAGACGGGATTGCGTTCGGATCTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCAt                                                                                                                                   >  2:1026770/1‑151 (MQ=255)                 aaggtGTGTTCGTCAGACGGGATTGCGTTCGGATCTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACg                                                                                                                               >  2:1097913/1‑150 (MQ=255)                      gtgtTCGTCAGACGGGATTGCGTTCGGATCTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGtt                                                                                                                         >  2:503909/1‑151 (MQ=255)                            gTCAGACGGGATTGCGTTCGGATCTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCg                                                                                                                   >  2:501019/1‑151 (MQ=255)                             tCAGACGGGATTGCGTTCGGATCTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCgg                                                                                                                  >  1:618254/1‑151 (MQ=255)                               aGACGGGATTGCGTTCGGATCTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTg                                                                                                                >  1:87454/1‑151 (MQ=255)                                gACGGGATTGCGTTCGGATCTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTg                                                                                                                >  2:1004806/1‑150 (MQ=255)                                gACGGGATTGCGTTCGGATCTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGa                                                                                                               <  1:1074381/151‑1 (MQ=255)                                  cgtgATGGCGTTCGGATCTTTCGCGCCGGGATCAGAAGCCCACAGCAGCACCCCGGTGTTGACATCAAATCCACCGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAAGTGATCCGGCCATCACGATGGTTGTATCGGTGATa                                                                                                             <  2:15466/148‑1 (MQ=255)                                  cGGGATTGCGTTCGGATCTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGAt                                                                                                              >  1:228476/1‑150 (MQ=255)                                   gggATTGCGTTCGGATCTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATaa                                                                                                            <  2:646662/151‑1 (MQ=255)                                     gATTGCGTTCGGATCTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATc                                                                                                          >  1:637139/1‑151 (MQ=255)                                      aTTGCGTTCGGATCTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCg                                                                                                         <  1:345953/151‑1 (MQ=255)                                         gCGTTCGGATCTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTg                                                                                                      <  1:891793/151‑1 (MQ=255)                                             tCGGATCTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCg                                                                                                   <  1:177151/150‑1 (MQ=255)                                              cGGATCTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGaa                                                                                                 <  1:791620/151‑1 (MQ=255)                                              cGGATCTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGa                                                                                                  <  2:638324/150‑1 (MQ=255)                                              cGGATCTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGa                                                                                                  <  1:172034/150‑1 (MQ=255)                                               ggATCTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAg                                                                                                <  1:987756/151‑1 (MQ=255)                                                gATCTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGt                                                                                               >  2:568251/1‑151 (MQ=255)                                                      tCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGTAACTGAACCTGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGtt                                                                                          <  1:611808/150‑1 (MQ=255)                                                       cgcgCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTc                                                                                         <  1:987284/150‑1 (MQ=255)                                                        gcgcCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCAt                                                                                       >  2:672968/1‑151 (MQ=255)                                                                    aaaGCCCACAGCAGCTCCCCGGTGTTGACATCAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCATACAGACCCGGtt                                                                           >  2:757173/1‑151 (MQ=255)                                                                       gCCCACAGCAGCTCCCCGGTGTTGACATCAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCATACAGACCCGGTTTgg                                                                        <  1:1087894/151‑1 (MQ=255)                                                                            cagcagCTCCCCGGTGTTGACATCAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCATACAGACCCGGTTTGGTGTCt                                                                   >  1:116893/1‑151 (MQ=255)                                                                            cagcagCTCCCCGGTGTTGACATCAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCATACAGACCCGGTTTGGTGTCt                                                                   >  2:617674/1‑151 (MQ=255)                                                                             agcagcTCCCCGGTGTTGACATCAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCATACAGACCCGGTTTGGTGTCTg                                                                  >  1:221224/1‑151 (MQ=255)                                                                             agcagcTCCCCGGTGTTGACATCAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCATACAGACCCGGTTTGGTGTCTg                                                                  <  1:105967/151‑1 (MQ=255)                                                                               cagcTCCCCGGTGTTGACATCAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCATACAGACCCGGTTTGGTGTCTGGc                                                                <  2:225650/151‑1 (MQ=255)                                                                                        ggTGTTGACATCAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCATACAGACCCGGTTTGGTGTCTGACATATTGCtt                                                       >  2:947580/1‑151 (MQ=255)                                                                                             tGACATCAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCATACAGACCCGGTTTGGTGTCTGGCATATTGCTTTGCa                                                   <  1:287086/150‑1 (MQ=255)                                                                                              gACATCAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCATACAGACCCGGTTTGGTGTCTGGCATATTGCTTTGc                                                    >  1:1059527/1‑148 (MQ=255)                                                                                                   cAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCATACAGACCCGGTTTGGTGTCTGGCATATTGCTTTGCAGATTGAg                                            <  1:1005339/151‑1 (MQ=255)                                                                                                   cAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCATACAGACCCGGTTTGGTGTCTGGCATATTGCTTTGCAGATTGAg                                            <  1:152255/151‑1 (MQ=255)                                                                                                    aaaaCCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCATACAGACCCGGTTTGGTGTCTGGCATATTGCTTTGCAGATTGAGc                                           <  2:518694/151‑1 (MQ=255)                                                                                                    aaaaCCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCATACAGACCCGGTTTGGTGTCTGGCATATTGCTTTGCAGATTGAGc                                           >  2:674164/1‑151 (MQ=255)                                                                                                        ccACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCATACAGACCCGGTTTGGTGTCTGGCATATTGCTTTGCAGATTGAGCACGc                                       >  1:372669/1‑151 (MQ=255)                                                                                                         cACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCATACAGACCCGGTTTGGTGTCTGGCATATTGCTTTGCAGATTGAGCACGcc                                      >  2:1111093/1‑151 (MQ=255)                                                                                                            ggATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCATACAGACCCGGTTTGGTGTCTGGCATATTGCTTTGCAGATTGAGCACGCCttt                                   <  1:395570/151‑1 (MQ=255)                                                                                                            ggATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCATACAGACCCGGTTTGGTGTCTGGCATATTGCTTTGCAGATTGAGCACGCCttt                                   <  2:67218/151‑1 (MQ=255)                                                                                                              aTCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCATACAGACCCGGTTTGGTGTCTGGCATATTGCTTTGCAGATTGAGCACGCCTTTAt                                 <  1:876022/151‑1 (MQ=255)                                                                                                                cACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCATACAGACCCGGTTTGGTGTCTGGCATATTGCTTTGCAGATTGAGCACGCCTCTATTg                               >  1:1024198/1‑151 (MQ=255)                                                                                                                 gaaacAGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCATACAGACCCGGTTTGGTGTCTGGCATATTGCTTTGCAGATTGAGCACGCCTTTATTgg                              >  2:351780/5‑151 (MQ=255)                                                                                                                      aGACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCATACAGACCCGGTTTGGTGTCTGGCATATTGCTTTGCAGATTGAGCACGCCTTTATTGGCGAAg                         <  2:212060/151‑1 (MQ=255)                                                                                                                       gACGTTTCGCGGGTTGAGAAGTTATCGGTAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCATACAGACCCGGTTTGGTGTCTGGCATATTGCTTTGCAGATTGAGCACGCCTTTATTGGCGAAgg                        >  1:743558/1‑151 (MQ=255)                                                                                                                       gACGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCATACAGACCCGGTTTGGTGTCTGGCATATTGCTTTGCAGATTGAGCACGCCTTTATTGGCGAAgg                        >  2:890138/1‑151 (MQ=255)                                                                                                                         cGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCATACAGACCCGGTTTGGTGTCTGGCATATTGCTTTGCAGATTGAGCACGCCTTTATTGGCGAAGGtt                      >  2:140929/1‑151 (MQ=255)                                                                                                                         cGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCATACAGACCCGGTTTGGTGTCTGGCATATTGCTTTGCAGATTGAGCACGCCTTTATTGGCGAAGGtt                      <  2:453257/151‑1 (MQ=255)                                                                                                                         cGTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCATACAGACCCGGTTTGGTGTCTGGCATATTGCTTTGCAGATTGAGCACGCCTTTATTGGCGAAGGtt                      <  1:949572/151‑1 (MQ=255)                                                                                                                          gTTTCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCATACAGACCCGGTTTGGTGTCTGGCATATTGCTTTGCAGATTGAGCACGCCTTTATTGGCGAAGGttt                     >  2:623070/1‑151 (MQ=255)                                                                                                                            ttCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCATACAGACCCGGTTTGGTGTCTGGCATATTGCTTTGCAGATTGAGCACGCCTTTATTGGCGAAGGTTTc                    <  2:152228/150‑1 (MQ=255)                                                                                                                            ttCGCGGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCATACAGACCCGGTTTGGTGTCTGGCATATTGCTTTGCAGATTGAGCACGCCTTTATTGGCGAAGGTTTCg                   >  2:192595/1‑151 (MQ=255)                                                                                                                                cgGGTTGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCATACAGACCCGGTTTGGTGTCTGGCATATTGCTTTGCAGATTGAGCACGCCTTTATTGGCGAAGGTTTCGcac                >  2:1004603/1‑150 (MQ=255)                                                                                                                                     tGAGAAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCATACAGACCCGGTTTGGTGTCTGGCATATTGCTTTGCAGATTGAGCACGCCTTTATTGGCGAAGGTTTCGCACAGTTTg          >  2:584584/1‑151 (MQ=255)                                                                                                                                       agaAGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCATACAGACCCGGTTTGGTGTCTGGCATATTGCTTTGCAGATTGAGCACGCCTTTATTGGCGAAGGTTTCGCACAGTTTGcc        >  1:1047184/1‑151 (MQ=255)                                                                                                                                         aaGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCATACAGACCCGGTTTGGTGTCTGGCATATTGCTTTGCAGATTGAGCACGCCTTTATTGGCGAAGGTTTCGCACAGTTTGCCGt      >  2:715909/1‑151 (MQ=255)                                                                                                                                          aGTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCATACAGACCCGGTTTGGTGTCTGGCATATTGCTTTGCAGATTGAGCACGCCTTTATTGGCGAAGGTTTCGCACAGTTTGCCGtt     >  2:800066/1‑151 (MQ=255)                                                                                                                                           gTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCATACAGACCCGGTTTGGTGTCTGGCATATTGCTTTGCAGATTGAGCACGCCTTTATTGGCGAAGGTTTCGCACAGTTTGCCGttt    <  2:304653/151‑1 (MQ=255)                                                                                                                                           gTTATCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCATACAGACCCGGTTTGGTGTCTGGCATATTGCTTTGCAGATTGAGCACGCCTTTATTGGCGAAGGTTTCGCACAGTTTGCCGttt    >  1:536723/1‑151 (MQ=255)                                                                                                                                              aTCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCATACAGACCCGGTTTGGTGTCTGGCATATTGCTTTGCAGATTGAGCACGCCTTTATTGGCGAAGGTTTCGCACAGTTTGCCGtttt   >  1:864355/1‑149 (MQ=255)                                                                                                                                              aTCGGGAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCATACAGACCCGGTTTGGTGTCTGGCATATTGCTTTGCAGATTGAGCACGCCTTTATTGGCGAAGGTTTCGCACAGTTTGCCGTTTTc  <  1:418793/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                 TGGCGAGTTAAAGGTAAAGGTGTGTTCGTCAGACGGGATTGCGTTCGGATCTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGTAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCATACAGACCCGGTTTGGTGTCTGGCATATTGCTTTGCAGATTGAGCACGCCTTTATTGGCGAAGGTTTCGCACAGTTTGCCGTTTTC  >  NC_000913/140023‑140313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑