Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,032,395	A→C	E412A (GAA→GCA)	pepQ →	proline dipeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,032,395	0	A	C	100.0%	295.6 / NA	72	E412A (GAA→GCA)	pepQ	proline dipeptidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (34/38);  total (34/38)

GGCAAAATATCCGTACCTGCGCTGCACCCGTATTCTCCAGCCGGGCATGGTGTTAACCATCGAACCGGGTATCTACTTCATCGAATCGCTACTGGCACCGTGGCGTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGAAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACGAAAACAACGTGGAAAACATGACCCGGGATCTGAAACTGGCGTGATGGAAAGCTGGTTAATTCCTGCGGCACCGGTCACGGTCGTTGAAGAG  >  NC_000913/4032249‑4032539                                                                                                                                                   |                                                                                                                                                 ggCAAAATATCCGTACCTGCGCTGCACCCGTATTCTCCAGCCGGGCATGGTGTTAACCATCGAACCGGGTATCTACTTCATCGAATCGCTACTGGCACCGTGGCGTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGc                                                                                                                                               >  1:275531/1‑150 (MQ=255)   cAAAATATCCGTACCTGCGCTGCACCCGTATTCTCCAGCCGGGCATGGTGTTAACCATCGAACCGGGTATCTACTTCATCGAATCGCTACTGGCACCGTGGCGTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCAc                                                                                                                                             >  2:628580/1‑150 (MQ=255)    aaaaTATCCGTACCTGCGCTGCACCCGTATTCTCCAGCCGGGCATGGTGTTAACCATCGAACCGGGTATCTACTTCATCGAATCGCTACTGGCACCGTGGCGTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTg                                                                                                                                           >  2:334621/1‑151 (MQ=255)    aaaaTATCCGTACCTGCGCTGCACCCGTATTCTCCAGCCGGGCATGGTGTTAACCATCGAACCGGGTATCTACTTCATCGAATCGCTACTGGCACCGTGGCGTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTg                                                                                                                                           <  2:644888/151‑1 (MQ=255)    aaaaTATCCGTACCTGCGCTGCACCCGTATTCTCCAGCCGGGCATGGTGTTAACCATCGAACCGGGTATCTACTTCATCGAATCGCTACTGGCACCGTGGCGTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTg                                                                                                                                           >  2:138020/1‑151 (MQ=255)         atCCGTACCTGCGCTGCACCCGTATTCTCCAGCCGGGCATGGTGTTAACCATCGAACCGGGTATCTACTTCATCGAATCGCTACTGGCACCGTGGCGTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAAcc                                                                                                                                      >  1:652028/1‑151 (MQ=255)         atCCGTACCTGCGCTGCACCCGTATTCTCCAGCCGGGCATGGTGTTAACCATCGAACCGGGTATCTACTTCATCGAATCGCTACTGGCACCGTGGCGTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAAcc                                                                                                                                      <  1:466630/151‑1 (MQ=255)            cGTACCTGCGCTGCACCCGTATTCTCCAGCCGGGCATGGTGTTAACCATCGAACCGGGTATCTACTTCATCGAATCGCTACTGGCACCGTGGCGTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGtt                                                                                                                                   <  1:280776/151‑1 (MQ=255)               aCCTGCGCTGCACCCGTATTCTCCAGCCGGGCATGGTGTTAACCATCGAACCGGGTATCTACTTCATCGAATCGCTACTGGCACCGTGGCGTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTcgg                                                                                                                                >  1:428545/1‑151 (MQ=255)               aCCTGCGCTGCACCCGTATTCTCCAGCCGGGCATGGTGTTAACCATCGAACCGGGTATCTACTTCATCGAATCGCTACTGGCACCGTGGCGTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTcgg                                                                                                                                >  2:841011/1‑151 (MQ=255)                ccTGCGCTGCACCCGTATTCTCCAGCCGGGCATGGTGTTAACCATCGAACCGGGTATCTACTTCATCGAATCGCTACTGGCACCGGGGCGTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTcgg                                                                                                                                >  1:91580/1‑150 (MQ=255)                 cTGCGCTGCACCCGTATTCTCCAGCCGGGCATGGTGTTAACCATCGAACCGGGTATCTACTTCATCGAATCGCTACTGGCACCGTGGCGTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTcggcg                                                                                                                              >  2:1028089/1‑151 (MQ=255)                   gcgcTGCACCCGTATTCTCCAGCCGGGCATGGTGTTAACCATCGAACCGGGTATCTACTTCATCGAATCGCTACTGGCACCGTGGCGTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTcggcg                                                                                                                              <  1:18561/149‑1 (MQ=255)                    cgcTGCACCCGTATTCTCCAGCCGGGCATGGTGTTAACCATCGAACCGGGTATCTACTTCATCGAATCGCTACTGGCACCGTGGCGTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCa                                                                                                                           <  1:173699/151‑1 (MQ=255)                         cACCCGTATTCTCCAGCCGGGCATGGTGTTAACCATCGAACCGGGTATCTACTTCATCGAATCGCTACTGGCACCGTGGCGTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTc                                                                                                                        <  1:489459/149‑1 (MQ=255)                          aCCCGTATTCTCCAGCCGGGCATGGTGTTAACCATCGAACCGGGTATCTACTTCATCGAATCGCTACTGGCACCGTGGCGTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTa                                                                                                                     <  2:803000/151‑1 (MQ=255)                               tATTCTCCAGCCGGGCATGGTGTTAACCATCGAACCGGGTATCTACTTCATCGAATCGCTACTGGCACCGTGGCGTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGa                                                                                                                 >  1:93730/1‑150 (MQ=255)                                  tctcCAGCCGGGCATGGTGTTAACCATCGAACCGGGTATCTACTTCATCGAATCGCTACTGGCACCGTGGCGTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGAc                                                                                                             >  1:154466/1‑151 (MQ=255)                                  tctcCAGCCGGGCATGGTGTTAACCATCGAACCGGGTATCTACTTCATCGAATCGCTACTGGCACCGTGGCGTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTAGCGAAGa                                                                                                              <  2:213315/150‑1 (MQ=255)                                        gCCGGGCATGGTGTTAACCATCGAACCGGGTATCTACTTCATCGAATCGCTACTGGCACCGTGGCGTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACgtg                                                                                                       <  2:652200/151‑1 (MQ=255)                                        gCCGGGCATGGTGTTAACCATCGAACCGGGTATCTACTTCATCGAATCGCTACTGGCACCGTGGCGTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACgtg                                                                                                       >  2:436516/1‑151 (MQ=255)                                         ccGGGCATGGTGTTAACCATCGAACCGGGTATCTACTTCATCGAATCGCTACTGGCACCGTGGCGTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACgtgg                                                                                                      >  2:675006/1‑151 (MQ=255)                                           gggCATGGTGTTAACCATCGAACCGGGTATCTACTTCATCGAATCGCTACTGGCACCGTGGCGTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACgtggtg                                                                                                    <  1:757994/151‑1 (MQ=255)                                           gggCATGGTGTTAACCATCGAACCGGGTATCTACTTCATCGAATCGCTACTGGCACCGTGGCGTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACgtggtg                                                                                                    >  2:121496/1‑151 (MQ=255)                                             gCATGGTGTTAACCATCGAACCGGGTATCTACTTCATCGAATCGCTACTGGCACCGTGGCGTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGAt                                                                                                  <  2:1011639/151‑1 (MQ=255)                                             gCATGGTGTTAACCATCGAACCGGGTATCTACTTCATCGAATCGCTACTGGCACCGTGGCGTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGAt                                                                                                  <  1:422138/151‑1 (MQ=255)                                                  gtgtTAACCATCGAACCGGGTATCTACTTCATCGAATCGCTACTGGCACCGTGGCGTGAAGGGCAGTTCAGCAAGCTCTTCAACTGGCAGAAAATTGCAGCGCTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCa                                                                                               <  2:1031282/149‑1 (MQ=255)                                                  gtgtTAACCATCGAACCGGGTATCTACTTCATCGAATCGCTACTGGCACCGTGGCGTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACg                                                                                             <  1:578095/151‑1 (MQ=255)                                                       aaCCATCGAACCGGGTATCTACTTCATCGAATCGCTACTGGCACCGTGGCGTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACGaaaa                                                                                         <  2:180826/150‑1 (MQ=255)                                                       aaCCATCGAACCGGGTATCTACTTCATCGAATCGCTACTGGCACCGTGGCGTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACGAAaac                                                                                        <  2:924071/151‑1 (MQ=255)                                                         ccATCGAACCGGGTATCTACTTCATCGAATCGCTACTGGCACCGTGGCGTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACGAAaaca                                                                                       >  2:881969/1‑150 (MQ=255)                                                           aTCGAACCGGGTATCTACTTCATCGAATCGCTACTGGCACCGTGGCGTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACGAAAACAACg                                                                                    <  1:794943/151‑1 (MQ=255)                                                             cGAACCGGGTATCTACTTCATCGAATCGCTACTGGCACCGTGGCGTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACGAAAACAACGTg                                                                                  <  2:1019739/151‑1 (MQ=255)                                                                    ggTATCTACTTCATCGAATCGCTACTGGCACCGTGGCGTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACGAAAACAACGTGGAAAACa                                                                           <  2:655612/151‑1 (MQ=255)                                                                     gTATCTACTTCATCGAATCGCTACTGGCACCGTGGCGTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACGAAAACAACGTGGAAAACa                                                                           >  2:629417/1‑150 (MQ=255)                                                                     gTATCTACTTCATCGAATCGCTACTGGCACCGTGGCGTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACGAAAACAACGTGGAAAACAt                                                                          >  2:84211/1‑151 (MQ=255)                                                                          tACTTCATCGAATCGCTACTGGCACCGTGGCGTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACGAAAACAACGTGGAAAACATGAccc                                                                     >  1:507969/1‑151 (MQ=255)                                                                                aTCGAATCGCTACTGGCACCGTGGCGTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACGAAAACAACGTGGAAAACATGACCCGGGATc                                                               >  1:860883/1‑151 (MQ=255)                                                                                       cGCTACTGGCACCGTGGCGTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACGAAAACAACGTGGAAAACATGACCCGGGATCTGAAACt                                                        >  2:1034103/1‑151 (MQ=255)                                                                                           aCTGGCACCGTGGCGTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACGAAAACAACGTGGAAAACATGACCCGGGATCTGAAACTGGc                                                     <  2:118988/150‑1 (MQ=255)                                                                                            cTGGCACCGTGGCGTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACGAAAACAACGTGGAAAACATGACCCGGGATCTGAAACTGGCGt                                                   >  1:352842/1‑151 (MQ=255)                                                                                                  ccGTGGCGTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACGAAAACAACGTGGAAAACATGACCCGGGATCTGAAACTGGCGTGATgg                                              >  1:31116/1‑150 (MQ=255)                                                                                                   cGTGGCGTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACGAAAACAACGTGGAAAACATGACCCGGGATCTGAAACTGGCGGGATGGa                                             >  2:299961/1‑150 (MQ=255)                                                                                                        cGTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACGAAAACAACGTGGAAAACATGACCCGGGATCTGAAACTGGCGTGATGGAAAGCTg                                       >  2:533159/1‑151 (MQ=255)                                                                                                        cGTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACGAAAACAACGTGGAAAACATGACCCGGGATCTGAAACTGGCGTGATGGAAAGCTg                                       >  1:276171/1‑151 (MQ=255)                                                                                                         gTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACGAAAACAACGTGGAAAACATGACCCGGGATCTGAAACTGGCGTGATGGAAAGCTgg                                      >  2:974223/1‑151 (MQ=255)                                                                                                         gTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACGAAAACAACGTGGAAAACATGACCCGGGATCTGAAACTGGCGTGATGGAAAGCTgg                                      >  1:864860/1‑151 (MQ=255)                                                                                                           gAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACGAAAACAACGTGGAAAACATGACCCGGGATCTGAAACTGGCGTGATGGAAAGCTGGtt                                    <  2:188613/151‑1 (MQ=255)                                                                                                           gAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACGAAAACAACGTGGAAAACATGACCCGGGATCTGAAACTGGCGTGATGGAAAGCTGGtt                                    >  2:414723/1‑151 (MQ=255)                                                                                                            aaGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACGAAAACAACGTGGAAAACATGACCCGGGATCTGAAACTGGCGTGATGGAAAGCTGGtt                                    >  2:763292/1‑150 (MQ=255)                                                                                                             aGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACGAAAACAACGTGGAAAACATGACCCGGGATCTGAAACTGGCGTGATGGAAAGCTGGTTaa                                  <  2:618024/151‑1 (MQ=255)                                                                                                                   gTTCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACGAAAACAACGTGGAAAACATGACCCGGGATCTGAAACTGGCGTGATGGAAAGCTGGTTAATTCCTg                            <  2:696233/151‑1 (MQ=255)                                                                                                                    ttCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACGAAAACAACGTGGAAAACATGACCCGGGATCTGAAACTGGCGTGATGGAAAGCTGGTTAATTCCTGc                           >  1:315694/1‑151 (MQ=255)                                                                                                                     tCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACGAAAACAACGTGGAAAACATGACCCGGGATCTGAAACTGGCGTGATGGAAAGCTGGTTAATTCCTGc                           <  2:937110/150‑1 (MQ=255)                                                                                                                     tCAGCAAGCACTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACGAAAACAACGTGGAAAACATGACCCGGGATCTGAAACTGGCGTGATGGAAAGCTGGTTAATTCCTGc                           <  2:455471/150‑1 (MQ=255)                                                                                                                         caagcaCTTCAACTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACGAAAACAACGTGGAAAACATGACCCGGGATCTGAAACTGGCGTGATGGAAAGCTGGTTAATTCCTGCGGCAc                      <  1:577081/151‑1 (MQ=255)                                                                                                                                ttCAACTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACGAAAACAACGTGGAAAACATGACCCGGGATCTGAAACTGGCGTGATGGAAAGCTGGTTAATTCCTGCGGCACCGGTCAc               >  2:695015/1‑151 (MQ=255)                                                                                                                                ttCAACTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACGAAAACAACGTGGAAAACATGACCCGGGATCTGAAACTGGCGTGATGGAAAGCTGGTTAATTCCTGCGGCACCGGTCAc               >  1:603387/1‑151 (MQ=255)                                                                                                                                 tCAACTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACGAAAACAACGTGGAAAACATGACCCGGGATCTGAAACTGGCGTGATGGAAAGCTGGTTAATTCCTGCGGCACCGGTCAc               <  2:46992/150‑1 (MQ=255)                                                                                                                                  cAACTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACGAAAACAACGTGGAAAACATGACCCGGGATCTGAAACTGGCGTGATGGAAAGCTGGTTAATTCCTGCGGCACCGGTCACgg             <  1:334671/151‑1 (MQ=255)                                                                                                                                   aaCTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACGAAAACAACGTGGAAAACATGACCCGGGATCTGAAACTGGCGTGATGGAAAGCTGGTTAATTCCTGCGGCACCGGTCACGGt            >  1:120416/1‑151 (MQ=255)                                                                                                                                    aCTGGCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACGAAAACAACGTGGAAAACATGACCCGGGATCTGAAACTGGCGTGATGGAAAGCTGGTTAATTCCTGCGGCACCGGTCACGGTc           <  1:1127754/151‑1 (MQ=255)                                                                                                                                       ggCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACGAAAACAACGTGGAAAACATGACCCGGGATCTGAAACTGGCGTGATGGAAAGCTGGTTAATTCCTGCGGCACCGGTCACGGTCg          <  2:422268/149‑1 (MQ=255)                                                                                                                                       ggCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACGAAAACAACGTGGAAAACATGACCCGGGATCTGAAACTGGCGTGATGGAAAGCTGGTTAATTCCTGCGGCACCGGTCACGGTCGt         <  2:574646/150‑1 (MQ=255)                                                                                                                                        gCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACGAAAACAACGTGGAAAACATGACCCGGGATCTGAAACTGGCGTGATGGAAAGCTGGTTAATTCCTGCGGCACCGGTCACGGTCGTTg       <  2:1065236/151‑1 (MQ=255)                                                                                                                                        gCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACGAAAACAACGTGGAAAACATGACCCGGGATCTGAAACTGGCGTGATGGAAAGCTGGTTAATTCCTGCGGCACCGGTCACGGTCGTTg       <  1:121511/151‑1 (MQ=255)                                                                                                                                        gCAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACGAAAACAACGTGGAAAACATGACCCGGGATCTGAAACTGGCGTGATGGAAAGCTGGTTAATTCCTGCGGAACCGGTCACGGTCGTTg       <  1:143246/151‑1 (MQ=255)                                                                                                                                         cAGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACGAAAACAACGTGGAAAACATGACCCGGGATCTGAAACTGGCGTGATGGAAAGCTGGTTAATTCCTGCGGCACCGGTCACGGTCGTTGa      <  1:875378/151‑1 (MQ=255)                                                                                                                                          aGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACGAAAACAACGTGGAAAACATGACCCGGGATCTGAAACTGGCGTGATGGAAAGCTGGTTAATTCCTGCGGCACCGGTCACGGTCGTTGaa     <  1:673356/151‑1 (MQ=255)                                                                                                                                          aGAAAATTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACGAAAACAACGTGGAAAACATGACCCGGGATCTGAAACTGGCGTGATGGAAAGCTGGTTAATTCCTGCGGCACCGGTCACGGTCGTTGaa     <  1:789715/151‑1 (MQ=255)                                                                                                                                            aaaaTTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACGAAAACAACGTGGAAAACATGACCCGGGATCTGAAACTGGCGTGATGGAAAGCTGGTTAATTCCTGCGGCACCGGTCACGGTCGTTGa      >  2:196572/1‑148 (MQ=255)                                                                                                                                             aaaTTGCAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACGAAAACAACGTGGAAAACATGACCCGGGATCTGAAACTGGCGTGATGGAAAGCTGGTTAATTCCTGCGGCACCGGTCACGGTCGTTGAagag  <  2:142217/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                 GGCAAAATATCCGTACCTGCGCTGCACCCGTATTCTCCAGCCGGGCATGGTGTTAACCATCGAACCGGGTATCTACTTCATCGAATCGCTACTGGCACCGTGGCGTGAAGGGCAGTTCAGCAAGCACTTCAACTGGCAGAAAATTGAAGCACTGAAACCGTTCGGCGGCATTCGTATCGAAGACAACGTGGTGATCCACGAAAACAACGTGGAAAACATGACCCGGGATCTGAAACTGGCGTGATGGAAAGCTGGTTAATTCCTGCGGCACCGGTCACGGTCGTTGAAGAG  >  NC_000913/4032249‑4032539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑