Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,180,393 A→C E133A (GAA→GCA)  rplJ → 50S ribosomal subunit protein L10

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,180,3930AC98.6% 290.6 / ‑3.4 71E133A (GAA→GCA) rplJ50S ribosomal subunit protein L10
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base C (26/44);  total (26/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.28e-01

CTATGGAACACCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTG  >  NC_000913/4180249‑4180540
                                                                                                                                                |                                                                                                                                                   
ctATGGAACACCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACgcagaa                                                                                                                                                 <  1:105730/149‑1 (MQ=255)
ctATGGAACACCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGc                                                                                                                                               >  2:928663/1‑151 (MQ=255)
  aTGGAACACCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCa                                                                                                                                              >  1:141027/1‑150 (MQ=255)
       acacCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCa                                                                                                                                        <  2:496244/151‑1 (MQ=255)
        cacCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCAc                                                                                                                                       >  2:1081679/1‑151 (MQ=255)
               gcgcTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGa                                                                                                                                 >  1:524171/1‑150 (MQ=255)
               gcgcTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGAt                                                                                                                                <  2:824085/151‑1 (MQ=255)
                    gctgctCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGc                                                                                                                             <  1:260191/149‑1 (MQ=255)
                    gctgctCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGc                                                                                                                             <  1:377991/149‑1 (MQ=255)
                      tgctCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAAcc                                                                                                                         >  1:1015940/1‑151 (MQ=255)
                       gctCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAAcc                                                                                                                         >  2:1115747/1‑150 (MQ=255)
                         tCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATg                                                                                                                      >  2:979739/1‑151 (MQ=255)
                          cGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATg                                                                                                                      >  2:291211/1‑150 (MQ=255)
                          cGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGa                                                                                                                     <  1:293884/151‑1 (MQ=255)
                          cGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGa                                                                                                                     <  2:1114235/151‑1 (MQ=255)
                           gTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGa                                                                                                                     >  1:1129734/1‑150 (MQ=255)
                           gTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGa                                                                                                                     >  2:623320/1‑150 (MQ=255)
                               gTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAaag                                                                                                                  <  1:657906/149‑1 (MQ=255)
                               gTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAaag                                                                                                                  <  1:990898/149‑1 (MQ=255)
                                    aaGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTc                                                                                                           <  1:20959/151‑1 (MQ=255)
                                     agagTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACTATGAAAGAAGCTTCg                                                                                                          >  1:963958/1‑151 (MQ=255)
                                      gagTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCgg                                                                                                         >  2:742021/1‑151 (MQ=255)
                                          tCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTgg                                                                                                     >  1:1010725/1‑151 (MQ=255)
                                          tCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTg                                                                                                      <  1:526403/150‑1 (MQ=255)
                                           cgcgAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTgg                                                                                                     <  2:1054274/150‑1 (MQ=255)
                                              gAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCaaa                                                                                                 <  2:64490/151‑1 (MQ=255)
                                                    gAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGtt                                                                                           <  2:537262/151‑1 (MQ=255)
                                                        gCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGt                                                                                        >  1:478420/1‑150 (MQ=255)
                                                        gCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTa                                                                                       <  1:873359/151‑1 (MQ=255)
                                                             aTTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTg                                                                                  <  2:257163/151‑1 (MQ=255)
                                                             aTTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTg                                                                                  <  1:241488/151‑1 (MQ=255)
                                                             aTTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTg                                                                                  <  2:1122216/151‑1 (MQ=255)
                                                                tGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGgc                                                                                <  1:674180/150‑1 (MQ=255)
                                                                 gAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGgctg                                                                              <  1:72879/151‑1 (MQ=255)
                                                                      cAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGt                                                                          <  2:999108/150‑1 (MQ=255)
                                                                        aaGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTAcg                                                                       >  1:834282/1‑151 (MQ=255)
                                                                         aGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTAcgc                                                                      >  1:513215/1‑151 (MQ=255)
                                                                            cGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGAt                                                                   <  1:736088/151‑1 (MQ=255)
                                                                             gCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATg                                                                  >  2:188993/1‑151 (MQ=255)
                                                                                gCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCg                                                                <  2:175086/150‑1 (MQ=255)
                                                                                    ttGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGaaa                                                             <  2:1085501/149‑1 (MQ=255)
                                                                                    ttGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGaaa                                                             <  1:388752/149‑1 (MQ=255)
                                                                                    ttGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAaag                                                            <  1:953333/150‑1 (MQ=255)
                                                                                      gAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAaagaa                                                          <  1:352186/150‑1 (MQ=255)
                                                                                            gAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGCtt                                                   <  2:900610/151‑1 (MQ=255)
                                                                                              gCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGCTTaa                                                 <  1:541914/151‑1 (MQ=255)
                                                                                                 gATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGCTTAAt                                                <  2:544326/149‑1 (MQ=255)
                                                                                                 gATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGCTTAATCg                                              >  1:134974/1‑151 (MQ=255)
                                                                                                   tCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGc                                             >  1:1083783/1‑150 (MQ=255)
                                                                                                   tCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGc                                             >  2:877589/1‑150 (MQ=255)
                                                                                                       ggCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGtt                                         >  1:612566/1‑150 (MQ=255)
                                                                                                              cAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCttttt                                 <  2:136772/151‑1 (MQ=255)
                                                                                                              cAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCttttt                                 <  2:394027/151‑1 (MQ=255)
                                                                                                              cAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCtttt                                  <  1:819871/150‑1 (MQ=255)
                                                                                                              cAGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCttt                                   <  2:601461/149‑1 (MQ=255)
                                                                                                               aGATCGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTa                                <  1:852667/151‑1 (MQ=255)
                                                                                                                   cGACCGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGc                            >  1:315009/1‑151 (MQ=255)
                                                                                                                      ccGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCAtt                         <  1:909722/151‑1 (MQ=255)
                                                                                                                      ccGCCTGGCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCAtt                         <  1:398106/151‑1 (MQ=255)
                                                                                                                            ggCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTa                   <  1:928777/151‑1 (MQ=255)
                                                                                                                             gCAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTAc                  <  2:1129608/151‑1 (MQ=255)
                                                                                                                              cAACTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACg                 <  1:228752/151‑1 (MQ=255)
                                                                                                                               aaCTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGt                <  2:813945/151‑1 (MQ=255)
                                                                                                                                aCTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGt                >  2:823069/1‑150 (MQ=255)
                                                                                                                                aCTCTGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGt                >  1:581960/1‑150 (MQ=255)
                                                                                                                                   ctGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGtat              <  2:992790/149‑1 (MQ=255)
                                                                                                                                    tGCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATaa            >  2:39938/1‑150 (MQ=255)
                                                                                                                                     gCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATaaa           <  1:858750/150‑1 (MQ=255)
                                                                                                                                     gCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATaa            <  2:1107090/149‑1 (MQ=255)
                                                                                                                                     gCCGACCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAAc          <  2:396791/151‑1 (MQ=255)
                                                                                                                                         aCCTACGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTAt      >  2:908917/1‑151 (MQ=255)
                                                                                                                                             aCGCAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTg  <  1:423125/151‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
CTATGGAACACCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTG  >  NC_000913/4180249‑4180540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: