Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,182,313	A→C	N357H (AAC→CAC)	rpoB →	RNA polymerase, beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,182,313	0	A	C	100.0%	251.8 / NA	65	N357H (AAC→CAC)	rpoB	RNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (30/35);  total (30/35)

GAGCTGATCTGCGCAGCGAACATGGAGCTGAGCCTGGATCTGCTGGCTAAGCTGAGCCAGTCTGGTCACAAGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTC  >  NC_000913/4182166‑4182459                                                                                                                                                    |                                                                                                                                                   gAGCTGATCTGCGCAGCGAACATGGAGCTGAGCCTGGATCTGCTGGCTAAGCTGAGCCAGTCTGGTCACAAGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTCACg                                                                                                                                                 >  2:94678/1‑151 (MQ=255) gAGCTGATCTGCGCAGCGAACATGGAGCTGAGCCTGGATCTGCTGGCTAAGCTGAGCCAGTCTGGTCACAAGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTCACg                                                                                                                                                 >  2:139462/1‑151 (MQ=255)  aGCTGATCTGCGCAGCGAACATGGAGCTGAGCCTGGATCTGCTGGCTAAGCTGAGCCAGTCTGGTCACAAGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTCACg                                                                                                                                                 >  2:1063831/1‑150 (MQ=255)     tGATCTGCGCAGCGAACATGGAGCTGAGCCTGGATCTGCTGGCTAAGCTGAGCCAGTCTGGTCACAAGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTCACGACCg                                                                                                                                             >  2:878490/1‑151 (MQ=255)     tGATCTGCGCAGCGAACATGGAGCTGAGCCTGGATCTGCTGGCTAAGCTGAGCCAGTCTGGTCACAAGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTCACGACCg                                                                                                                                             >  1:263308/1‑151 (MQ=255)      gATCTGCGCAGCGAACATGGAGCTGAGCCTGGATCTGCTGGCTAAGCTGAGCCAGTCTGGTCACAAGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTCACGAcc                                                                                                                                              <  2:797601/149‑1 (MQ=255)           gcgcAGCGAACATGGAGCTGAGCCTGGATCTGCTGGCTAAGCTGAGCCAGTCTGGTCACAAGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTCACGACCGt                                                                                                                                            <  2:963036/146‑1 (MQ=255)           gcgcAGCGAACATGGAGCTGAGCCTGGATCTGCTGGCTAAGCTGAGCCAGTCTGGTCACAAGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTCACGACCGTCTg                                                                                                                                         <  2:886915/149‑1 (MQ=255)           gcgcAGCGAACATGGAGCTGAGCCTGGATCTGCTGGCTAAGCTGAGCCAGTCTGGTCACAAGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTCACGACCGTCTGa                                                                                                                                        <  2:491557/150‑1 (MQ=255)           gcgcAGCGAACATGGAGCTGAGCCTGGATCTGCTGGCTAAGCTGAGCCAGTCTGGTCACAAGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGAACCAACTCACGACCGTc                                                                                                                                           <  1:1010010/147‑1 (MQ=255)            cgcAGCGAACATGGAGCTGAGCCTGGATCTGCTGGCTAAGCTGAGCCAGTCTGGTCACAAGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTCACGACCGTCTGAgc                                                                                                                                      <  2:384610/151‑1 (MQ=255)              cAGCGAACATGGAGCTGAGCCTGGATCTGCTGGCTAAGCTGAGCCAGTCTGGTCACAAGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTCACGACCGTCTGAgcgc                                                                                                                                    >  2:300821/1‑151 (MQ=255)               aGCGAACATGGAGCTGAGCCTGGATCTGCTGGCTAAGCTGAGCCAGTCTGGTCACAAGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTCACGACCGTCTGAGCGCa                                                                                                                                   <  1:89173/151‑1 (MQ=255)                 cGAACATGGAGCTGAGCCTGGATCTGCTGGCTAAGCTGAGCCAGTCTGGTCACAAGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTCACGACCGTCTGAGCGCACt                                                                                                                                 <  1:733745/151‑1 (MQ=255)                   aaCATGGAGCTGAGCCTGGATCTGCTGGCTAAGCTGAGCCAGTCTGGTCACAAGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTCACGACCGTCTGAGCGCACTgg                                                                                                                               >  1:995141/1‑151 (MQ=255)                     cATGGAGCTGAGCCTGGATCTGCTGGCTAAGCTGAGCCAGTCTGGTCACAAGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTCACGACCGTCTGAGCGCACTGGt                                                                                                                              >  2:1067133/1‑150 (MQ=255)                         gAGCTGAGCCTGGATCTGCTGGCTAAGCTGAGCCAGTCTGGTCACAAGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTCACGACCGTCTGAGCGCACTGGTAGaa                                                                                                                          >  2:314447/1‑150 (MQ=255)                          aGCTGAGCCTGGATCTGCTGGCTAAGCTGAGCCAGTCTGGTCACAAGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTCACGACCGTCTGAGCGCACTGGTAGAAAt                                                                                                                        >  1:582756/1‑151 (MQ=255)                          aGCTGAGCCTGGATCTGCTGGCTAAGCTGAGCCAGTCTGGTCACAAGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTCACGACCGTCTGAGCGCACTGGTAGAAAt                                                                                                                        >  1:620150/1‑151 (MQ=255)                                 ccTGGATCTGCTGGCTAAGCTGAGCCAGTCTGGTCACAAGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTCACGACCGTCTGAGCGCACTGGTAGAAATCTACCGc                                                                                                                 <  2:8465/151‑1 (MQ=255)                                   tGGATCTGCTGGCTAAGCTGAGCCAGTCTGGTCACAAGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTCACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCat                                                                                                               >  2:916377/1‑151 (MQ=255)                                    ggATCTGCTGGCTAAGCTGAGCCAGTCTGGTCACAAGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTCACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCatg                                                                                                              >  2:521784/1‑151 (MQ=255)                                          gctgGCTAAGCTGAGCCAGTCTGGTCACAAGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTCACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATgcg                                                                                                         <  1:1037057/150‑1 (MQ=255)                                              gCTAAGCTGAGCCAGTCTGGTCACAAGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTCACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTg                                                                                                    <  2:799635/151‑1 (MQ=255)                                              gCTAAGCTGAGCCAGTCTGGTCACAAGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTCACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTg                                                                                                    <  1:161957/151‑1 (MQ=255)                                                 aaGCTGAGCCAGTCTGGTCACAAGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTCACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGc                                                                                                  <  2:66985/150‑1 (MQ=255)                                                 aaGCTGAGCCAGTCTGGTCACAAGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTCACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGc                                                                                                  <  1:1119452/150‑1 (MQ=255)                                                      gAGCCAGTCTGGTCACAAGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTCACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAgccg                                                                                            >  2:450515/1‑151 (MQ=255)                                                      gAGCCAGTCTGGTCACAAGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTCACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAgccg                                                                                            <  2:994255/151‑1 (MQ=255)                                                         ccAGTCTGGTCACAAGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTCACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGccgccg                                                                                         >  1:868860/1‑151 (MQ=255)                                                           aGTCTGGTCACAAGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTCACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGAc                                                                                       >  2:1075223/1‑151 (MQ=255)                                                             acTGGTCACAAGCGTATCGAAACGCTCTTCACCAACGATCTGGATCACGGCCCATATATCTCTGCAACCTTACGTGTCGACCCACCTCACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGa                                                                                        >  2:698562/2‑148 (MQ=255)                                                                ggTCACAAGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTCACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTc                                                                                     <  1:793892/148‑1 (MQ=255)                                                                 gTCACAAGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTCACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTg                                                                                  >  2:675365/1‑150 (MQ=255)                                                                 gTCACAAGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTCACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGa                                                                                 <  2:309624/151‑1 (MQ=255)                                                                   cacaAGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTCACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGaa                                                                                <  2:67275/150‑1 (MQ=255)                                                                     caAGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTCACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAagca                                                                             <  1:980521/151‑1 (MQ=255)                                                                         cGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTCACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTg                                                                         >  1:1092210/1‑151 (MQ=255)                                                                           tATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTCACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTGa                                                                        >  2:887145/1‑150 (MQ=255)                                                                                 aaCGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTCACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTg                                                                 <  2:374025/151‑1 (MQ=255)                                                                                 aaCGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTCACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTg                                                                 <  1:224772/151‑1 (MQ=255)                                                                                               aCGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTCACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGtt                                                   <  1:24620/151‑1 (MQ=255)                                                                                                cGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTCACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGttc                                                  >  2:910562/1‑151 (MQ=255)                                                                                                      ggATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTCACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGttctt                                                <  2:1104213/147‑1 (MQ=255)                                                                                                      ggATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTCACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTtcttct                                              <  1:83213/149‑1 (MQ=255)                                                                                                      ggATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTCACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTtctc                                             <  1:272070/150‑1 (MQ=255)                                                                                                      ggATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTCACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTcc                                            <  2:163173/151‑1 (MQ=255)                                                                                                       gATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTCACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCg                                           <  2:890537/151‑1 (MQ=255)                                                                                                               cccATATATCTCTGAAACCTTACGTGTCGACCCAACTCACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGt                                   >  2:482453/1‑151 (MQ=255)                                                                                                                      atCTCTGAAACCTTACGTGTCGACCCAACTCACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGAc                             <  2:666575/150‑1 (MQ=255)                                                                                                                      atCTCTGAAACCTTACGTGTCGACCCAACTCACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACt                            >  1:105145/1‑151 (MQ=255)                                                                                                                          ctGAAACCTTACGTGTCGACCCAACTCACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTc                        >  2:455351/1‑151 (MQ=255)                                                                                                                          ctGAAACCTTACGTGTCGACCCAACTCACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTc                        >  1:641887/1‑151 (MQ=255)                                                                                                                            gAAACCTTACGTGTCGACCCAACTCACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTc                        <  1:979017/149‑1 (MQ=255)                                                                                                                            gAAACCTTACGTGTCGACCCAACTCACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTg                      <  1:71442/151‑1 (MQ=255)                                                                                                                            gAAACCTTACGTGTCGACCCAACTCACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTg                      >  2:317058/1‑151 (MQ=255)                                                                                                                              aaCCTTACGTGTCGACCCAACTCACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCg                    >  2:429441/1‑151 (MQ=255)                                                                                                                                ccTTACGTGTCGACCCAACTCACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGt                  <  1:446146/151‑1 (MQ=255)                                                                                                                                   tACGTGTCGACCCAACTCACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTgg               >  2:832037/1‑151 (MQ=255)                                                                                                                                    aCGTGTCGACCCAACTCACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTgg               <  1:1026752/150‑1 (MQ=255)                                                                                                                                    aCGTGTCGACCCAACTCACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGt              <  2:241566/151‑1 (MQ=255)                                                                                                                                      gtgtCGACCCAACTCACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCg            >  2:461845/1‑151 (MQ=255)                                                                                                                                            aCCCAACTCACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGaa      <  2:672610/151‑1 (MQ=255)                                                                                                                                               cAACTCACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGtt   <  1:972842/151‑1 (MQ=255)                                                                                                                                                 aCTCACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTc  >  1:403315/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                                   GAGCTGATCTGCGCAGCGAACATGGAGCTGAGCCTGGATCTGCTGGCTAAGCTGAGCCAGTCTGGTCACAAGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTC  >  NC_000913/4182166‑4182459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑