Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,586,506	A→C	L86V (TTA→GTA)	hsdR ←	endonuclease R Type I restriction enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,586,506	0	A	C	100.0%	224.3 / NA	57	L86V (TTA→GTA)	hsdR	endonuclease R Type I restriction enzyme
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (33/24);  total (33/24)

AACACAAACACCGGCACCGGGAAGTCATAATCTTTAGTGACCAGACGGTAGTACCAGACAGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATT  >  NC_000913/4586358‑4586648                                                                                                                                                     |                                                                                                                                               aaCACAAACACCGGCACCGGGAAGTCATAATCTTTAGTGACCAGACGGTAGTACCAGACAGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACtt                                                                                                                                              >  1:923551/1‑151 (MQ=255) aaCACAAACACCGGCACCGGGAAGTCATAATCTTTAGTGACCAGACGGTAGTACCAGACAGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACtt                                                                                                                                              <  2:255183/151‑1 (MQ=255)     caAACACCGGCACCGGGAAGTCATAATCTTTAGTGACCAGACGGTAGTACCAGACAGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTtgtg                                                                                                                                          <  2:828441/151‑1 (MQ=255)      aaaCACCGGCACCGGGAAGTCATAATCTTTAGTGACCAGACGGTAGTACCAGACAGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTtgtg                                                                                                                                          >  1:801741/1‑150 (MQ=255)        acacCGGTTCCGGGAAGTCATAATCTTTAGTGACCAGACGGTAGTACCAGTCCTCCAGGCGGAACCCGTGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCCCCGCCTGGTTACCAATGCGGCGTTCTTTGTGaa                                                                                                                                        <  2:1102531/150‑1 (MQ=255)         caccggcaccggGAAGTCATAATCTTTAGTGACCAGACGGTAGTACCAGACAGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGAAAt                                                                                                                                      >  1:676705/1‑151 (MQ=255)                 ccggGAAGTCATAATCTTTAGTGACCAGACGGTAGTACCAGACAGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGAAATACagag                                                                                                                                >  1:67633/1‑149 (MQ=255)                       aGTCATAATCCTTAGTGACCAGACGGTAGTACCAGACAGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGAAATACAGAGAGGAtgtt                                                                                                                        <  2:238270/151‑1 (MQ=255)                             aaTCTTTAGTGACCAGACGGTAGTACCAGACAGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGtcatc                                                                                                                  >  2:172439/1‑151 (MQ=255)                                cTTTAGTGACCAGACGGTAGTACCAGACAGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGTCATcaac                                                                                                               >  2:284150/1‑151 (MQ=255)                                  ttAGTGACCAGACGGTAGTACCAGACAGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGTCATcaaca                                                                                                              <  2:313806/150‑1 (MQ=255)                                    aGTGACCAGACGGTAGTACCAGACAGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGtcatca                                                                                                                 <  2:835010/145‑1 (MQ=255)                                    aGTGACCAGACGGTAGTACCAGACAGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGTCATcaacaa                                                                                                             >  2:422902/1‑149 (MQ=255)                                    aGTGACCAGACGGTAGTACCAGACAGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAg                                                                                                           <  1:822892/151‑1 (MQ=255)                                           aGACGGTAGTACCAGACAGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGAttt                                                                                                    <  1:395672/151‑1 (MQ=255)                                           aGACGGTAGTACCAGACAGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGAttt                                                                                                    >  2:790064/1‑151 (MQ=255)                                              cGGTAGTACCAGACAGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGc                                                                                                 >  1:109279/1‑151 (MQ=255)                                                 tagtaCCAGACAGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCg                                                                                               >  1:697627/1‑150 (MQ=255)                                                 tagtaCCAGACAGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCg                                                                                               >  2:934195/1‑150 (MQ=255)                                                      ccAGACAGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTc                                                                                          <  2:12314/150‑1 (MQ=255)                                                       cAGACAGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCAc                                                                                        >  2:125825/1‑151 (MQ=255)                                                           cAGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGc                                                                                      >  1:549882/1‑149 (MQ=255)                                                            aGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAgg                                                                                   >  1:1134306/1‑151 (MQ=255)                                                             gCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGGAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAgg                                                                                   <  2:1072621/150‑1 (MQ=255)                                                              ccAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGgag                                                                                 <  1:891139/151‑1 (MQ=255)                                                               cAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGgaga                                                                                <  2:59295/151‑1 (MQ=255)                                                                aGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAgg                                                                                   >  1:971563/1‑147 (MQ=255)                                                                aGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGAt                                                                               <  2:68990/151‑1 (MQ=255)                                                                     gAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCgtgt                                                                          >  1:148424/1‑151 (MQ=255)                                                                     gAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCgtgt                                                                          <  2:264392/151‑1 (MQ=255)                                                                      aaCCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGtt                                                                         >  1:182958/1‑151 (MQ=255)                                                                           gAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATtc                                                                    >  2:823519/1‑151 (MQ=255)                                                                                    gCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAggg                                                           <  2:20052/151‑1 (MQ=255)                                                                                      acaTCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAggggg                                                         >  2:82635/1‑151 (MQ=255)                                                                                       caTCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAgggggg                                                        >  1:1000319/1‑151 (MQ=255)                                                                                       caTCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAgggggg                                                        >  2:1069014/1‑151 (MQ=255)                                                                                           tGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGt                                                    >  1:324225/1‑151 (MQ=255)                                                                                           tGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGt                                                    >  1:161057/1‑151 (MQ=255)                                                                                            gggCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGa                                                       <  2:375196/147‑1 (MQ=255)                                                                                            gggCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGAt                                                      <  1:354863/148‑1 (MQ=255)                                                                                                aTCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGt                                               <  2:676615/151‑1 (MQ=255)                                                                                                   gTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAAc                                            >  1:328655/1‑151 (MQ=255)                                                                                                     tGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAAc                                            >  1:411564/1‑149 (MQ=255)                                                                                                     tGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACa                                           <  1:395055/150‑1 (MQ=255)                                                                                                      gagaTCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAAc                                            >  2:139598/1‑148 (MQ=255)                                                                                                        gaTCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGAcc                                       <  1:380660/151‑1 (MQ=255)                                                                                                        gaTCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGAcc                                       <  2:401024/151‑1 (MQ=255)                                                                                                           cGTTATGATATTCGTGCACCGACTGGTTACCAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCa                                      >  1:1092655/1‑149 (MQ=255)                                                                                                               aTGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATg                                 <  2:483609/150‑1 (MQ=255)                                                                                                                tGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGtt                               >  2:873420/1‑151 (MQ=255)                                                                                                                  atatTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTc                             <  2:523942/151‑1 (MQ=255)                                                                                                                   tatTCGTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCg                            >  2:174892/1‑151 (MQ=255)                                                                                                                        gTGCACCGCCTGGTTACCAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCtgtg                       >  1:99103/1‑151 (MQ=255)                                                                                                                                   ggTTACCAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCaa             >  2:274107/1‑150 (MQ=255)                                                                                                                                      tACCAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGACATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGGAACAGACCAGGATGTTTGGCTGTGGCTTCGCCAAACa          >  2:161643/1‑150 (MQ=255)                                                                                                                                        ccAATGCGGCGTACTTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACAt         <  2:614421/149‑1 (MQ=255)                                                                                                                                             gcggcgTACTTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCAtt  >  1:796077/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                               AACACAAACACCGGCACCGGGAAGTCATAATCTTTAGTGACCAGACGGTAGTACCAGACAGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATT  >  NC_000913/4586358‑4586648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑