Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 522,892 A→C intergenic (+62/‑369) ybbP → / → rhsD putative ABC transporter permease/Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913522,8920AC98.4% 258.1 / ‑2.9 64intergenic (+62/‑369)ybbP/rhsDputative ABC transporter permease/Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base C (34/29);  total (35/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.69e-01

GAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATATGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCTATTGCTGATATTAATCTGAATCGGTGGAGTTTATTGATTTTAAATATAGCCCTACAAAAATAACTCAGACTAATAAAATCATAAATCATATGCGTTGAATGG  >  NC_000913/522745‑523039
                                                                                                                                                   |                                                                                                                                                   
gAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATCTGt                                                                                                                                                  <  2:674491/151‑1 (MQ=255)
   cgcTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATCTGTTTc                                                                                                                                               >  2:205887/1‑151 (MQ=255)
       gctgctgTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATCTGTTTCATtg                                                                                                                                           <  1:925155/151‑1 (MQ=255)
          gctgTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATCTGTTTCATtgat                                                                                                                                         <  1:758379/150‑1 (MQ=255)
          gctgTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATCTGTTTCATtgat                                                                                                                                         <  2:1042336/150‑1 (MQ=255)
           ctgTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATCTGTTTCATtgatg                                                                                                                                        >  2:490764/1‑150 (MQ=255)
                       ggcggcTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAAtt                                                                                                                           >  2:168315/1‑151 (MQ=255)
                         cggcTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTgg                                                                                                                         <  2:689233/151‑1 (MQ=255)
                           gctggctgGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTg                                                                                                                       >  2:692183/1‑151 (MQ=255)
                             tggctggGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGAt                                                                                                                     >  2:1040419/1‑151 (MQ=255)
                              ggctggGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGa                                                                                                                      <  1:4480/149‑1 (MQ=255)
                              ggctggGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGAtt                                                                                                                    >  1:456359/1‑151 (MQ=255)
                              ggctggGAGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGAtt                                                                                                                    >  1:812166/1‑151 (MQ=255)
                               gctggGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGAttt                                                                                                                   >  1:864330/1‑151 (MQ=255)
                                 tggGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTcc                                                                                                                 >  1:660870/1‑151 (MQ=255)
                                  gggTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTcc                                                                                                                 <  2:812722/150‑1 (MQ=255)
                                   ggTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTa                                                                                                               <  1:376693/151‑1 (MQ=255)
                                        gcgACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCttt                                                                                                          >  1:699678/1‑151 (MQ=255)
                                          gACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCt                                                                                                        <  2:956539/151‑1 (MQ=255)
                                          gACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCt                                                                                                        >  2:254350/1‑151 (MQ=255)
                                            cTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCTAt                                                                                                      >  1:302423/1‑151 (MQ=255)
                                                     ggTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCTATTGCTGatat                                                                                             <  2:958482/151‑1 (MQ=255)
                                                        aaGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCTATTGCTGATATTa                                                                                           <  1:1110827/150‑1 (MQ=255)
                                                         aGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCTATTGCTGATATTAAt                                                                                         >  1:182278/1‑151 (MQ=255)
                                                          ggCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCTATTGCTGATATTAATc                                                                                        >  2:1105885/1‑151 (MQ=255)
                                                            cgcTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCTATTGCTGATATTAATCTg                                                                                      <  1:209102/151‑1 (MQ=255)
                                                             gcTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCTATTGCTGATATTAATCTGa                                                                                     <  2:790104/151‑1 (MQ=255)
                                                                      gCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCTATTGCTGATATTAATCTGAATCGGTgg                                                                             >  1:543493/1‑150 (MQ=255)
                                                                           ttGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCTATTGCTGATATTAATCTGAATCGGTGGAGttt                                                                        <  2:86888/150‑1 (MQ=255)
                                                                                   tgatgaGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCTATTGCTGATATTAATCTGAATCGGTGGAGTTTATTGAtttt                                                               >  1:284493/1‑151 (MQ=255)
                                                                                       gagaGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCTATTGCTGATATTAATCTGAATCGGTGGAGTTTATTGATTTTAAat                                                           >  2:297216/1‑151 (MQ=255)
                                                                                         gagTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCTATTGCTGATATTAATCTGAATCGGTGGAGTTTATTGATTTTAAatat                                                         <  2:314855/151‑1 (MQ=255)
                                                                                           gTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCTATTGCTGATATTAATCTGAATCGGTGGAGTTTATTGATTTTAAATATAg                                                       <  1:814686/151‑1 (MQ=255)
                                                                                            tAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCTATTGCTGATATTAATCTGAATCGGTGGAGTTTATTGATTTTAAATATAGc                                                      >  2:376980/1‑151 (MQ=255)
                                                                                            tAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCTATTGCTGATATTAATCTGAATCGGTGGAGTTTATTGATTTTAAATATAGc                                                      >  1:1012908/1‑151 (MQ=255)
                                                                                             aaaTAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCTATTGCTGATATTAATCTGAATCGGTGGAGTTTATTGATTTTAAATATAGcc                                                     >  1:265455/1‑151 (MQ=255)
                                                                                             aaaTAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCTATTGCTGATATTAATCTGAATCGGTGGAGTTTATTGATTTTAAATATAGcc                                                     <  2:403620/151‑1 (MQ=255)
                                                                                              aaTAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCTATTGCTGATATTAATCTGAATCGGTGGAGTTTATTGATTTTAAATATAGccc                                                    >  1:200656/1‑151 (MQ=255)
                                                                                               aTAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCTATTGCTGATATTAATCTGAATCGGTGGAGTTTATTGATTTTAAATATAGCCCt                                                   <  2:7980/151‑1 (MQ=255)
                                                                                                tAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCTATTGCTGATATTAATCTGAATCGGTGGAGTTTATTGATTTTAAATATAGCCCt                                                   >  1:107470/1‑150 (MQ=255)
                                                                                                 aaGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCTATTGCTGATATTAATCTGAATCGGTGGAGTTTATTGATTTTAAATATAGCCCTa                                                  <  2:908754/150‑1 (MQ=255)
                                                                                                             ggTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCTATTGCTGATATTAATCTGAATCGGTGGAGTTTATTGATTTTAAATATAGCCCTACAAAAATAACTc                                      <  1:579131/150‑1 (MQ=255)
                                                                                                             ggTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCTATTGCTGATATTAATCTGAATCGGTGGAGTTTATTGATTTTAAATATAGCCCTACAAAAATAACTCa                                     <  1:638192/151‑1 (MQ=255)
                                                                                                              gTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCTATTGCTGATATTAATCTGAATCGGTGGAGTTTATTGATTTTAAATATAGCCCTACAAAAATAACTCa                                     <  1:1126684/150‑1 (MQ=255)
                                                                                                                 gAATGGAACGCCTTGTTTAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCTATTGCTGATATTAATCTGAATCGGTGGAGTTTATTGATTTTAAATATAGCCCTACAAAAATAACTCAGACt                                 <  1:747815/151‑1 (MQ=255)
                                                                                                                      gAACGCCTTGTTTAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCTATTGCTGATATTAATCTGAATCGGTGGAGTTTATTGATTTTAAATATAGCCCTACAAAAATAACTCAGACtaata                             >  2:691873/1‑150 (MQ=255)
                                                                                                                          gCCTTGTTTAAATTAATTCTACAATATGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCTATTGCTGATATTAATCTGAATCGGTGGAGTTTATTGATTTTAAATATAGCCCTACAAAAATAACTCAGACTAATAaaatc                        >  1:687286/1‑151 (MQ=255)
                                                                                                                           ccTTGTTTAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCTATTGCTGATATTAATCTGAATCGGTGGAGTTTATTGATTTTAAATATAGCCCTACAAAAATAACTCAGACTAATAaaatc                        >  1:104528/1‑150 (MQ=255)
                                                                                                                            cTTGTTTAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCTATTGCTGATATTAATCTGAATCGGTGGAGTTTATTGATTTTAAATATAGCCCTACAAAAATAACTCAGACTAATAaaatcat                      >  1:236217/1‑151 (MQ=255)
                                                                                                                               gTTTAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCTATTGCTGATATTAATCTGAATCGGTGGAGTTTATTGATTTTAAATATAGCCCTACAAAAATAACTCAGACTAATAaaatcataa                    >  1:534578/1‑150 (MQ=255)
                                                                                                                                 ttAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCTATTGCTGATATTAATCTGAATCGGTGGAGTTTATTGATTTTAAATATAGCCCTACAAAAATAACTCAGACTAATAaaatcataaatc                 >  2:723546/1‑151 (MQ=255)
                                                                                                                                 ttAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCTATTGCTGATATTAATCTGAATCGGTGGAGTTTATTGATTTTAAATATAGCCCTACAAAAATAACTCAGACTAATAaaatcataaatc                 >  1:1051070/1‑151 (MQ=255)
                                                                                                                                  tAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCTATTGCTGATATTAATCTGAATCGGTGGAGTTTATTGATTTTAAATATAGCCCTACAAAAATAACTCAGACTAATAaaatcataaatc                 <  2:615375/150‑1 (MQ=255)
                                                                                                                                  tAAATTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCTATTGCTGATATTAATCTGAATCGGTGGAGTTTATTGATTTTAAATATAGCCCTACAAAAATAACTCAGACTAATAaaatcataaatc                 >  2:370083/1‑150 (MQ=255)
                                                                                                                                   aaaTTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCTATTGCTGATATTAATCTGAATCGGTGGAGTTTATTGATTTTAAATATAGCCCTACAAAAATAACTCAGACTAATAaaatcataaatcat               >  1:369641/1‑151 (MQ=255)
                                                                                                                                   aaaTTAATTCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCTATTGCTGATATTAATCTGAATCGGTGGAGTTTATTGATTTTAAATATAGCCCTACAAAAATAACTCAGACTAATAaaatcataaatca                <  2:513769/150‑1 (MQ=255)
                                                                                                                                    aattaattCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCTATTGCTGATATTAATCTGAATCGGTGGAGTTTATTGATTTTAAATATAGCCCTACAAAAATAACTCAGACTAATAaaatcataaatcat               >  1:22756/1‑150 (MQ=255)
                                                                                                                                       taattCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCTATTGCTGATATTAATCTGAATCGGTGGAGTTTATTGATTTTAAATATAGCCCTACAAAAATAACTCAGACTAATAAAATCATAAATCATATGc           >  2:739545/1‑151 (MQ=255)
                                                                                                                                        aattCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCTATTGCTGATATTAATCTGAATCGGTGGAGTTTATTGATTTTAAATATAGCCCTACAAAAATAACTCAGACTAATAAAATCATAAATCATATGc           <  1:225289/150‑1 (MQ=255)
                                                                                                                                        aattCTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCTATTGCTGATATTAATCTGAATCGGTGGAGTTTATTGATTTTAAATATAGCCCTACAAAAATAACTCAGACTAATAAAATCATAAATCATATGCg          >  2:397778/1‑151 (MQ=255)
                                                                                                                                            cTACAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCTATTGCTGATATTAATCTGAATCGGTGGAGTTTATTGATTTTAAATATAGCCCTACAAAAATAACTCAGACTAATAAAATCATAAATCATATGCGTTg       >  1:5383/1‑150 (MQ=255)
                                                                                                                                              aCAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCTATTGCTGATATTAATCTGAATCGGTGGAGTTTATTGATTTTAAATATAGCCCTACAAAAATAACTCAGACTAATAAAATCATAAATCATATGCGTTg       <  1:149048/148‑1 (MQ=255)
                                                                                                                                              aCAATCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCTATTGCTGATATTAATCTGAATCGGTGGAGTTTATTGATTTTAAATATAGCCCTACAAAAATAACTCAGACTAATAAAATCATAAATCATATGCGTTGaa     <  1:273319/150‑1 (MQ=255)
                                                                                                                                                aaTCTGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCTATTGCTGATATTAATCTGAATCGGTGGAGTTTATTGATTTTAAATATAGCCCTACAAAAATAACTCAGACTAATAAAATCATAAATCATATGCGTTGAATgg  <  1:878504/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GAGCGCTGCTGCTGTCGCTTTTCGGCGGCTGGCTGGGTGCGCGACTGGTTAAGGGTAAGGCGCTGTTCAGGCAGTTTGCGGGGTGATGAGAGTAAATAAGGGGCGTTCTGGTTGAATGGAACGCCTTGTTTAAATTAATTCTACAATATGTTTCATTGATGATGTTATTGAATTGGTGATTTCCTATCTTTCTATTGCTGATATTAATCTGAATCGGTGGAGTTTATTGATTTTAAATATAGCCCTACAAAAATAACTCAGACTAATAAAATCATAAATCATATGCGTTGAATGG  >  NC_000913/522745‑523039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: