Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,756,434	A→G	D246G (GAC→GGC)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,756,434	0	A	G	100.0%	265.3 / NA	72	D246G (GAC→GGC)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/39);  total (33/39)

GTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAA  >  NC_000913/1756287‑1756579                                                                                                                                                    |                                                                                                                                                  gTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCt                                                                                                                                                >  2:239255/1‑151 (MQ=255)   tCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTgg                                                                                                                                              >  2:669271/1‑151 (MQ=255)      gACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTggg                                                                                                                                             <  2:369462/149‑1 (MQ=255)      gACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTgg                                                                                                                                              <  1:626478/148‑1 (MQ=255)      gACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGgtg                                                                                                                                           >  2:147335/1‑151 (MQ=255)       aCGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGgtgt                                                                                                                                          >  2:397465/1‑151 (MQ=255)           tATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGa                                                                                                                                       <  2:855878/150‑1 (MQ=255)           tATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGa                                                                                                                                       <  2:225758/150‑1 (MQ=255)            aTCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGaa                                                                                                                                      <  1:128339/150‑1 (MQ=255)            aTCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGaa                                                                                                                                      <  2:131786/150‑1 (MQ=255)              cGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATc                                                                                                                                   <  1:969273/151‑1 (MQ=255)              cGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATc                                                                                                                                   >  2:912828/1‑151 (MQ=255)              cGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATc                                                                                                                                   >  2:829455/1‑151 (MQ=255)               gAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATcc                                                                                                                                  >  2:173345/1‑151 (MQ=255)                aaaTCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATccc                                                                                                                                 <  1:203866/151‑1 (MQ=255)                aaaTCCGTGAGCACCTGAAAGCGCACGGCGGCCAAAACAGCCACATCATCTGCAAAAGCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGGGGCGGCCTGGGTGTAGAAATc                                                                                                                                   <  1:61114/149‑1 (MQ=255)                  aTCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCgg                                                                                                                               <  1:1071019/151‑1 (MQ=255)                    ccGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGt                                                                                                                              >  1:957685/1‑150 (MQ=255)                        gAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTagaa                                                                                                                          <  2:108095/150‑1 (MQ=255)                         aGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTagaaga                                                                                                                        <  2:878409/151‑1 (MQ=255)                            aCCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGt                                                                                                                     <  2:710318/151‑1 (MQ=255)                             ccTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGtt                                                                                                                    >  2:1070396/1‑151 (MQ=255)                                gAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTAt                                                                                                                  <  2:507753/150‑1 (MQ=255)                                gAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATc                                                                                                                 >  1:1036833/1‑151 (MQ=255)                                   aGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTc                                                                                                              <  1:194238/151‑1 (MQ=255)                                   aGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTc                                                                                                              >  1:82796/1‑151 (MQ=255)                                         cggcggCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCag                                                                                                        <  1:28775/151‑1 (MQ=255)                                          ggcggcGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGccc                                                                                                          >  1:462364/1‑148 (MQ=255)                                           gcggcgAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCaga                                                                                                       >  2:935640/1‑150 (MQ=255)                                            cggcgAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCagaag                                                                                                     <  1:524235/151‑1 (MQ=255)                                            cggcgAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCaga                                                                                                       >  1:508049/1‑149 (MQ=255)                                              gcgAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAgat                                                                                                   <  1:953870/151‑1 (MQ=255)                                               cgAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAgatg                                                                                                  <  2:353823/151‑1 (MQ=255)                                               cgAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAgatg                                                                                                  >  2:706920/1‑151 (MQ=255)                                                 aaaaCATCCACATCATCGCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAgatgat                                                                                                <  2:499276/151‑1 (MQ=255)                                                  aaaCATCAACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAAACCCGGTAGAAGAAGTTATCTTCGCCCAGAAgatgat                                                                                                <  1:1016782/150‑1 (MQ=255)                                                   aaCATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAgatgatg                                                                                               >  1:865469/1‑150 (MQ=255)                                                     cATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATc                                                                                            <  1:204225/151‑1 (MQ=255)                                                     cATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATc                                                                                            >  2:1085888/1‑151 (MQ=255)                                                        ccACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGa                                                                                          <  1:159074/150‑1 (MQ=255)                                                           catcatCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGaaaa                                                                                       >  1:473657/1‑150 (MQ=255)                                                             tcatcTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTTTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAAt                                                                                     <  1:635958/150‑1 (MQ=255)                                                             tcatcTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGAAGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGAAGAAACCCCGGAAGAAGAAGTCAAATTCGCCCAGAAGATGAGGATCGAAAAATg                                                                                    >  1:516799/1‑151 (MQ=255)                                                                tctcCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTAt                                                                                 >  2:107227/1‑151 (MQ=255)                                                                         tCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGATTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCAc                                                                         >  2:292762/1‑150 (MQ=255)                                                                          cGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACg                                                                        <  2:1051200/150‑1 (MQ=255)                                                                             aaaCCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTaa                                                                     >  2:271931/1‑150 (MQ=255)                                                                                ccAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTc                                                                 <  2:319302/151‑1 (MQ=255)                                                                                 cAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCg                                                                <  1:397411/151‑1 (MQ=255)                                                                                   ggAAAGCCTCAACAACATCGACGAAATCCTCGAAGCGTCTGACAGCTTCATGGACACGCGAGGCCGACTGGGGGAAGGACTCGCGGTAGATTATGTTATCTTCGCCCAGAAGATGATGATGGAAAATTATATATAAGCACGTAAACTCGtt                                                              >  1:578876/1‑151 (MQ=255)                                                                                     aaGGCCACAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTAt                                                            >  1:160905/1‑151 (MQ=255)                                                                                      aGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTAt                                                            <  2:686981/150‑1 (MQ=255)                                                                                      aGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATc                                                           <  2:165665/151‑1 (MQ=255)                                                                                       ggCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCa                                                          <  2:1027982/151‑1 (MQ=255)                                                                                             aacaacTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCg                                                     >  1:488401/1‑150 (MQ=255)                                                                                                aacTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGAccc                                                 >  1:959990/1‑151 (MQ=255)                                                                                                       acgaAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCt                                          <  2:623083/151‑1 (MQ=255)                                                                                                        cgaAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGGCGTTATCACTGCGACCCAGATGCTg                                         <  1:634319/151‑1 (MQ=255)                                                                                                         gaAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTgg                                        <  1:113351/151‑1 (MQ=255)                                                                                                           aaTCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGAt                                      <  1:953171/151‑1 (MQ=255)                                                                                                                 cGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATg                                >  1:827932/1‑151 (MQ=255)                                                                                                                   aaGCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGAt                              <  1:435527/151‑1 (MQ=255)                                                                                                                     gCCTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCAt                                 >  2:121672/1‑146 (MQ=255)                                                                                                                      ccTCTGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCaa                           <  1:1023644/151‑1 (MQ=255)                                                                                                                        tctGACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCaaa                          >  2:858076/1‑150 (MQ=255)                                                                                                                           gACGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAAc                       <  2:1100942/150‑1 (MQ=255)                                                                                                                             cGGCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAAccc                     >  1:298359/1‑150 (MQ=255)                                                                                                                              ggCATCATGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCAc                   <  2:748270/151‑1 (MQ=255)                                                                                                                                 atcatGGTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGcc                >  2:513555/1‑151 (MQ=255)                                                                                                                                      ggTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGAc            <  1:920403/150‑1 (MQ=255)                                                                                                                                      ggTTGCGCGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACt           >  2:967591/1‑151 (MQ=255)                                                                                                                                          gcgcGTGGCGGCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTcgcg       >  2:1077071/1‑151 (MQ=255)                                                                                                                                                ggcggcCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGaa  <  1:854151/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                  GTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAA  >  NC_000913/1756287‑1756579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑