Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 87,381 C→T L9F (CTC→TTC)  ilvH → acetolactate synthase 3, small subunit, valine‑sensitive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091387,3810CT98.1% 185.5 / ‑3.8 52L9F (CTC→TTC) ilvHacetolactate synthase 3, small subunit, valine‑sensitive
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (24/27);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

GCGCAATAATCGCCTGGTGTTTGTTGATGTTACCGTCGATGGCAGCGAGCACGTCTACCCGATGCAGATTCGCGGGGGCGGAATGGATGAAATGTGGTTAAGCAAAACGGAGAGAACCTGATTATGCGCCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTG  >  NC_000913/87234‑87528
                                                                                                                                                   |                                                                                                                                                   
gcgcAATAATCGCCTGGTGTTTGTTGATGTTACCGTCGATGGCAGCGAGCACGTCTACCCGATGCAGATTCGCGGGGGCGGAATGGATGAAATGTGGTTAAGCAAAACGGAGAGAACCTGATTATGCGCCGGATATTATCAGTCTTATTc                                                                                                                                                   <  1:452117/150‑1 (MQ=255)
 cgcAATAATCGCCTGGTGTTTGTTGATGTTACCGTCGATGGCAGCGAGCACGTCTACCCGATGCAGATTCGCGGGGGCGGAATGGATGAAATGTGGTTAAGCAAAACGGAGAGAACCTGATTATGCGCCGGATATTATCAGTCTTATTCg                                                                                                                                                  >  2:634315/1‑150 (MQ=255)
    aataatCGCCTGGTGTTTGTTGATGTTACCGTCGATGGCAGCGAGCACGTCTACCCGATGCAGATTCGCGGGGGCGGAATGGATGAAATGTGGTTAAGCAAAACGGAGAGAACCTGATTATGCGCCGGATATTATCAGTCTTATTCGaaaa                                                                                                                                              <  2:958087/151‑1 (MQ=255)
    aataatCGCCTGGTGTTTGTTGATGTTACCGTCGATGGCAGCGAGCACGTCTACCCGATGCAGATTCGCGGGGGCGGAATGGATGAAATGTGGTTAAGCAAAACGGAGAGAACCTGATTATGCGCCGGATATTATCAGTCTTATTCGaaa                                                                                                                                               >  1:930683/1‑150 (MQ=255)
       aatCGCCTGGTGTTTGTTGATGTTACCGTCGATGGCAGCGAGCACGTCTACCCGATGCAGATTCGCGGGGGCGGAATGGATGAAATGTGGTTAAGCAAAACGGAGAGAACCTGATTATGCGCCGGATATTATCAGTCTTATTCGAAAATg                                                                                                                                            >  1:279152/1‑150 (MQ=255)
       aatCGCCTGGTGTTTGTTGATGTTACCGTCGATGGCAGCGAGCACGTCTACCCGATGCAGATTCGCGGGGGCGGAATGGATGAAATGTGGTTAAGCAAAACGGAGAGAACCTGATTATGCGCCGGATATTATCAGTCTTATTCGAAAATg                                                                                                                                            >  2:416976/1‑150 (MQ=255)
          cGCCTGGTGTTTGTTGATGTTACCGTCGATGGCAGCGAGCACGTCTACCCGATGCAGATTCGCGGGGGCGGAATGGATGAAATGTGGTTAAGCAAAACGGAGAGAACCTGATTATGCGCCGGATATTATCAGTCTTATTCGAAAATGAATc                                                                                                                                        <  1:600301/151‑1 (MQ=255)
                  gtttgttGATGTTACCGTCGATGGCAGCGAGCACGTCTACCCGATGCAGATTCGCGGGGGCGGAATGGATGAAATGTGGTTAAGCAAAACGGAGAGAACCTGATTATGCGCCGGATATTATCAGTCTTATTCGAAAATGAATCAGGcgcg                                                                                                                                 <  1:120533/150‑1 (MQ=255)
                  gtttgttGATGTTACCGTCGATGGCAGCGAGCACGTCTACCCGATGCAGATTCGCGGGGGCGGAATGGATGAAATGTGGTTAAGCAAAACGGAGAGAACCTGATTATGCGCCGGATATTATCAGTCTTATTCGAAAATGAATCAGGcgcg                                                                                                                                 <  2:366352/150‑1 (MQ=255)
                      gttgATGTTACCGTCGATGGCAGCGAGCACGTCTACCCGATGCAGATTCGCGGGGGCGGAATGGATGAAATGTGGTTAAGCAAAACGGAGAGAACCTGATTATGCGCCGGATATTATCAGTCTTATTCGAAAATGAATCAGGCGCGTTAt                                                                                                                             <  1:839971/150‑1 (MQ=255)
                          aTGTTACCGTCGATGGCAGCGAGCACGTCTACCCGATGCAGATTCGCGGGGGCGGAATGGATGAAATGTGGTTAAGCAAAACGGAGAGAACCTGATTATGCGCCGGATATTATCAGTCTTATTCGAAAATGAATCAGGCGCGTTATCCcgc                                                                                                                        <  1:471501/151‑1 (MQ=255)
                               aCCGTCGATGGCAGCGAGCACGTCTACCCGATGCAGATTCGCGGGGGCGGAATGGATGAAATGTGGTTAAGCAAAACGGAGAGAACCTGATTATGCGCCGGATATTATCAGTCTTATTCGAAAATGAATCAGGCGCGTTATCCCGCGTGa                                                                                                                    <  2:9317/150‑1 (MQ=255)
                                ccGTCGATGGCAGCGAGCACGTCTACCCGATGCAGATTCGCGGGGGCGGAATGGATGAAATGTGGTTAAGCAAAACGGAGAGAACCTGATTATGCGCCGGATATTATCAGTCTTATTCGAAAATGAATCAGGCGCGTTATCCCGCGTGAt                                                                                                                   <  2:928410/150‑1 (MQ=255)
                                  gTCGATGGCAGCGAGCACGTCTACCCGATGCAGATTCGCGGGGGCGGAATGGATGAAATGTGGTTAAGCAAAACGGAGAGAACCTGATTATGCGCCGGATATTATCAGTCTTATTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTgg                                                                                                                >  2:791676/1‑151 (MQ=255)
                                  gTCGATGGCAGCGAGCACGTCTACCCGATGCAGATTCGCGGGGGCGGAATGGATGAAATGTGGTTAAGCAAAACGGAGAGAACCTGATTATGCGCCGGATATTATCAGTCTTATTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTgg                                                                                                                <  1:58007/151‑1 (MQ=255)
                                    cGATGGCAGCGAGCACGTCTACCCGATGCAGATTCGCGGGGGCGGAATGGATGAAATGTGGTTAAGCAAAACGGAGAGAACCTGATTATGCGCCGGATATTATCAGTCTTATTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGcc                                                                                                              >  1:291921/1‑151 (MQ=255)
                                           aGCGAGCACGTCTACCCGATGCAGATTCGCGGGGGCGGAATGGATGAAATGTGGTTAAGCAAAACGGAGAGAACCTGATTATGCGCCGGATATTATCAGTCTTATTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTc                                                                                                       >  2:218717/1‑151 (MQ=255)
                                             cGAGCACGTCTACCCGATGCAGATTCGCGGGGGTGGAATGGATGAAATGTGGTTAAGCAAAACGGAGAGAACCTGATTATGCGCCGGATATTATCAGTCTTATTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTccc                                                                                                     <  2:706804/151‑1 (MQ=255)
                                              gAGCACGTCTACCCGATGCAGATTCGCGGGGGCGGAATGGATGAAATGTGGTTAAGCAAAACGGAGAGAACCTGATTATGCGCCGGATATTATCAGTCTTATTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCa                                                                                                    <  1:589412/151‑1 (MQ=255)
                                                    gTCTACCCGATGCAGATTCGCGGGGGCGGAATGGATGAAATGTGGTTAAGCAAAACGGAGAGAACCTGATTATGCGCCGGATATTATCAGTCTTATTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTgg                                                                                              >  1:29512/1‑151 (MQ=255)
                                                      cTACCCGATGCAGATTCGCGGGGGCGGAATGGATGAAATGTGGTTAAGCAAAACGGAGAGAACCTGATTATGCGCCGGATATTATCAGTCTTATTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCt                                                                                            >  1:765142/1‑151 (MQ=255)
                                                       tACCCGATGCAGATTCGCGGGGGCGGAATGGATGAAATGTGGTTAAGCAAAACGGAGAGAACCTGATTATGCGCCGGATATTATCAGTCTTATTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCt                                                                                            >  2:457157/1‑150 (MQ=255)
                                                         cccGATGCAGATTCGCGGGGGCGGAATGGATGAAATGTGGTTAAGCAAAACGGAGAGAACCTGATTATGCGCCGGATATTATCAGTCTTATTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTaca                                                                                         <  2:528402/151‑1 (MQ=255)
                                                                   aTTCGCGGGGGCGGAATGGATGAAATGTGGTTAAGCAAAACGGAGAGAACCTGATTATGCGCCGGATATTATCAGTCTTATTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGaa                                                                                 >  2:482031/1‑149 (MQ=255)
                                                                       gcgGGGGCGGAATGGATGAAATGTGGTTAAGCAAAACGGAGAGAACCTGATTATGCGCCGGATATTATCAGTCTTATTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTg                                                                           <  1:430848/151‑1 (MQ=255)
                                                                            ggCGGAATGGATGAAATGTGGTTAAGCAAAACGGAGAGAACCTGATTATGCGCCGGATATTATCAGTCTTATTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGAGTGGCTACAACATTGAAAGCCTGACCGt                                                                      >  1:591088/1‑151 (MQ=255)
                                                                              cGGAATGGATGAAATGTGGTTAAGCAAAACGGAGAGAACCTGATTATGCGCCGGATATTATCAGTCTTATTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTg                                                                    <  1:456219/151‑1 (MQ=255)
                                                                               ggAATGGATGAAATGTGGTTAAGCAAAACGGAGAGAACCTGATTATGCGCCGGATATTATCAGTCTTATTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTgc                                                                   <  2:724381/151‑1 (MQ=255)
                                                                                          aaTGTGGTTAAGCAAAACGGAGAGAACCTGATTATGCGCCGGATATTATCAGTCTTATTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACcgacg                                                        <  2:735637/151‑1 (MQ=255)
                                                                                           aTGTGGTTAAGCAAAACGGAGAGAACCTGATTATGCGCCGGATATTATCAGTCTTATTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACcgacga                                                       <  2:980583/151‑1 (MQ=255)
                                                                                               ggTTAAGCAAAACGGAGAGAACCTGATTATGCGCCGGATATTATCAGTCTTATTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCg                                                   <  2:291975/151‑1 (MQ=255)
                                                                                                gTTAAGCAAAACGGAGAGAACCTGATTATGCGCCGGATATTATCAGTCTTATTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCCACAACATTGAAAGCCTGACCGTTGCTCCATCCGACGGTcc                                                    <  1:321266/149‑1 (MQ=255)
                                                                                                     gCAAAACGGAGAGAACCTGATTATGCGCCGGATATTATCAGTCTTATTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGACAt                                               >  1:159714/1‑149 (MQ=255)
                                                                                                     gCAAAACGGAGAGAACCTGATTATGCGCCGGATATTATCAGTCTTATTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCC‑TTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGACATTAt                                            >  2:222014/1‑151 (MQ=255)
                                                                                                       aaaaCGGAGAGAACCTGATTATGCGCCGGATATTATCAGTCTTATTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCCACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGATATTATc                                           >  1:618007/1‑151 (MQ=255)
                                                                                                          aCGGAGAGAACCTGATTATGCGCCGGATATTATCAGTCTTATTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGACATTATcgcg                                        >  1:203303/1‑151 (MQ=255)
                                                                                                                 gaACCTGATTATGCGCCGGATATTATCAGTCTTATTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGACATTATCGCGTATGAcc                                 >  2:882758/1‑151 (MQ=255)
                                                                                                                   aCCTGATTATGCGCCGGATATTATCAGTCTTATTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGACATTATCGCGTATGACCAt                               >  1:968118/1‑151 (MQ=255)
                                                                                                                    ccTGATTATGCGCCGGATATTATCAGTCTTATTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGACATTATCGCGTATGACCATc                              <  1:446520/151‑1 (MQ=255)
                                                                                                                       gATTATGCGCCGGATATTATCAGTCTTATTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGACATTATCGCGTATGACCATcc                             <  2:371461/149‑1 (MQ=255)
                                                                                                                           aTGCGCCGGATATTATCAGTCTTATTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGACATTATCGCGTATGACCATCCAGACCg                       <  1:340778/151‑1 (MQ=255)
                                                                                                                              cgcCGGATATTATCAGTCTTATTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTgg                    >  2:483970/1‑151 (MQ=255)
                                                                                                                                  ggATATTATCAGTCTTATTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCg                 >  1:78617/1‑150 (MQ=255)
                                                                                                                                   gATATTATCAGTCTTATTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATAGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGa                >  1:213725/1‑150 (MQ=255)
                                                                                                                                     tattatCAGTCTTATTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATg              >  1:441215/1‑150 (MQ=255)
                                                                                                                                      attatCAGTCTTATTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGa             <  2:612608/150‑1 (MQ=255)
                                                                                                                                          tCAGTCTTATTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGaa            >  1:582930/1‑147 (MQ=255)
                                                                                                                                           cAGTCTTATTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAg        <  1:585127/150‑1 (MQ=255)
                                                                                                                                           cAGTCTTATTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGACATTATCGCGTATGACCAGCCAGACCGTGGGCGATGaaaaa         <  2:86920/149‑1 (MQ=255)
                                                                                                                                           cAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAg        >  1:45904/1‑150 (MQ=255)
                                                                                                                                            aGTCTTATTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCACCAACCGACGATCCGACATTATCGCGTATGCCCATCCAGTCCGTGGGCGATGAAAAAg        <  1:239757/149‑1 (MQ=255)
                                                                                                                                                ttATTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTg  >  1:382767/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GCGCAATAATCGCCTGGTGTTTGTTGATGTTACCGTCGATGGCAGCGAGCACGTCTACCCGATGCAGATTCGCGGGGGCGGAATGGATGAAATGTGGTTAAGCAAAACGGAGAGAACCTGATTATGCGCCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTG  >  NC_000913/87234‑87528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑