Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,338,928 C→A L107F (TTG→TTT)  mlaE ← ABC transporter maintaining OM lipid asymmetry, inner membrane permease protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,338,9280CA99.1% 461.1 / ‑3.6 114coding (321/783 nt)mlaEABC transporter maintaining OM lipid asymmetry, inner membrane permease protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (39/74);  minor base . (0/1);  total (39/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 120 total aligned reads displayed.

GTGGTAATGAAATAACCCCAGCCCAGAAACGGGGAGAAATAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGCAGCCCCAACACCATTCCGATGAACACGCCAGAAACCACAA  >  NC_000913/3338780‑3339073
                                                                                                                                                    |                                                                                                                                                 
gTGGTAATGAAATAACCCCAGCCCAGAAACGGGGAGAAATAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCaaaaaa                                                                                                                                                 >  1:295669/1‑151 (MQ=255)
gTGGTAATGAAATAACCCCAGCCCAGAAACGGGGAGAAATAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCaaaaaa                                                                                                                                                 >  2:1157366/1‑151 (MQ=255)
  ggTAATGAAATAACCCCAGCCCAGAAACGGGGAGAAATAACCCGGCGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAaaaaac                                                                                                                                                <  1:936851/150‑1 (MQ=255)
  ggTAATGAAATAACCCCAGCCCAGAAACGGGGAGAAATAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAaaaaaca                                                                                                                                               >  1:255599/1‑151 (MQ=255)
  ggTAATGAAATAACCCCAGCCCAGAAACGGGGAGAAATAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAaaaaaca                                                                                                                                               >  1:258060/1‑151 (MQ=255)
     aaTGAAATAACCCCAGCCCAGAAACGGGGAGAAATAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAA‑AACAAcgc                                                                                                                                           <  1:436360/151‑1 (MQ=255)
        gAAATAACCCCAGCCCAGAAACGGGGAGAAATAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCgg                                                                                                                                         >  1:491570/1‑151 (MQ=255)
        gAAATAACCCCAGCCCAGAAACGGGGAGAAATAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCgg                                                                                                                                         >  1:704220/1‑151 (MQ=255)
        gAAATAACCCCAGCCCAGAAACGGGGAGAAATAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCgg                                                                                                                                         >  1:440401/1‑151 (MQ=255)
        gAAATAACCCCAGCCCAGAAACGGGGAGAAATAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCgg                                                                                                                                         >  1:1268063/1‑151 (MQ=255)
         aaaTAACCCCAGCCCAGAAACGGGGAGAAATAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGCTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCgg                                                                                                                                         >  2:1009981/1‑150 (MQ=255)
          aaTAACCCCAGCCCAGAAACGGGGAGAAATAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCa                                                                                                                                       <  1:42254/151‑1 (MQ=255)
            tAACCCCAGCCCAGAAACGGGGAGAAAAAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAac                                                                                                                                     <  2:248571/151‑1 (MQ=255)
             aaCCCCAGCCCAGAAACGGGGAGAAATAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAacc                                                                                                                                    <  1:1157911/151‑1 (MQ=255)
               ccccAGCCCAGAAACGGGGAGAAATAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAaccac                                                                                                                                  <  1:1539725/151‑1 (MQ=255)
               ccccAGCCCAGAAACGGGGAGAAATAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAacca                                                                                                                                   >  2:1613727/1‑150 (MQ=255)
               ccccAGCCCAGAAACGGGGAGAAATAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAacca                                                                                                                                   >  1:74783/1‑150 (MQ=255)
                    gcccagAAACGGGGAGAAATAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGc                                                                                                                              >  1:201142/1‑150 (MQ=255)
                      ccagAAACGGGGAGAAATAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGcccc                                                                                                                           <  1:241245/151‑1 (MQ=255)
                         gAAACGGGGAGAAATAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGt                                                                                                                        >  1:1016181/1‑151 (MQ=255)
                                ggAGAAATAACCCGACGCCGGGGATCCACCGCCAGCATCTCCATACGGGAGAGTTGCCCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGc                                                                                                                  <  1:1538284/150‑1 (MQ=255)
                                ggAGAAATAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGc                                                                                                                  <  1:311047/150‑1 (MQ=255)
                                ggAAAAATAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGc                                                                                                                  <  1:1217784/150‑1 (MQ=255)
                                    aaaTAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAg                                                                                                             >  2:635482/1‑151 (MQ=255)
                                     aaTAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGc                                                                                                            <  1:851901/151‑1 (MQ=255)
                                       tAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGa                                                                                                          <  2:874897/151‑1 (MQ=255)
                                         aCCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATa                                                                                                        <  1:1468465/151‑1 (MQ=255)
                                         aCCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATa                                                                                                        >  1:1447522/1‑151 (MQ=255)
                                         aCCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATa                                                                                                        <  1:125128/151‑1 (MQ=255)
                                           ccGACGCAGCGGATCCCCCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAAc                                                                                                      <  1:994877/151‑1 (MQ=255)
                                           ccGACGCAGCGGATCCCCCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAAc                                                                                                      <  2:42041/151‑1 (MQ=255)
                                                    cGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCAGCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAg                                                                                              <  2:1028378/150‑1 (MQ=255)
                                                    cGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCAGCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGc                                                                                             <  1:1076957/151‑1 (MQ=255)
                                                      gATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCAt                                                                                           >  2:1321592/1‑151 (MQ=255)
                                                      gATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCAt                                                                                           >  2:1604004/1‑151 (MQ=255)
                                                        tCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATAc                                                                                         <  2:848455/151‑1 (MQ=255)
                                                        ccccccGCCATCAGCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATAc                                                                                         <  2:1233831/147‑1 (MQ=255)
                                                         ccACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATAcc                                                                                        <  1:1037830/151‑1 (MQ=255)
                                                          cACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATAccc                                                                                       <  2:1608087/151‑1 (MQ=255)
                                                          cACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATAccc                                                                                       <  1:160516/151‑1 (MQ=255)
                                                          cACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATAccc                                                                                       <  1:1043273/151‑1 (MQ=255)
                                                          cACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGACCGATTTCTGAGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGACCCAGTTCACGCAGTAGCGATAACGCCACCAGCATGccc                                                                                       <  2:795942/151‑1 (MQ=255)
                                                          cACCGCCATCATCTCCATACTGGAGAGTTGCTCTGGAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGACCCAACACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATGACGCCACCAGCGTAccc                                                                                       <  2:78271/151‑1 (MQ=255)
                                                               ccATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGAc                                                                                   <  1:606261/150‑1 (MQ=255)
                                                               ccATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGAc                                                                                   <  2:1241214/150‑1 (MQ=255)
                                                               ccATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGAc                                                                                   <  1:260774/150‑1 (MQ=255)
                                                                 atcatcTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTgg                                                                                >  1:182783/1‑151 (MQ=255)
                                                                   catcTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGtt                                                                              <  1:1346252/151‑1 (MQ=255)
                                                                     tctcCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGttt                                                                             >  2:506175/1‑150 (MQ=255)
                                                                     tctcCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGttt                                                                             >  1:897735/1‑150 (MQ=255)
                                                                     tctcCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGttt                                                                             >  2:981854/1‑150 (MQ=255)
                                                                      ctcCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTc                                                                            >  2:149215/1‑150 (MQ=255)
                                                                        ccATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCg                                                                          >  1:716413/1‑150 (MQ=255)
                                                                        ccATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCg                                                                          >  1:489722/1‑150 (MQ=255)
                                                                        ccATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCg                                                                          >  1:1318096/1‑150 (MQ=255)
                                                                        ccATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGc                                                                         <  1:323431/151‑1 (MQ=255)
                                                                        ccATACTGGAGAGTGGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGc                                                                         <  2:1485655/151‑1 (MQ=255)
                                                                          aTACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCa                                                                        >  1:480976/1‑150 (MQ=255)
                                                                                 agagTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAg                                                                 <  2:1075491/150‑1 (MQ=255)
                                                                                 agagTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAg                                                                 <  1:271701/150‑1 (MQ=255)
                                                                                 agagTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAg                                                                 <  2:1005094/150‑1 (MQ=255)
                                                                                 agagTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAg                                                                 <  2:1315155/150‑1 (MQ=255)
                                                                                  gagTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTg                                                               >  2:1020945/1‑151 (MQ=255)
                                                                                   agTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAGCCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCCCCAGCATACCCAGACTGGTTTCCGCACTATAAGTg                                                               <  2:1304770/150‑1 (MQ=255)
                                                                                     ttGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTc                                                            <  1:1021441/151‑1 (MQ=255)
                                                                                     ttGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTc                                                            <  1:1495445/151‑1 (MQ=255)
                                                                                       gCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAg                                                          >  2:1041339/1‑151 (MQ=255)
                                                                                       gCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAg                                                          >  2:856870/1‑151 (MQ=255)
                                                                                       gCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAg                                                          >  2:1557862/1‑151 (MQ=255)
                                                                                           tGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAAcc                                                      <  2:349041/151‑1 (MQ=255)
                                                                                              aGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCGGTCCACGCAGAAGCGAAAAGGCCACAAACATACCAAGACGGGTTTCCGCACCAAAAGGGGCCAAAACCAGa                                                   >  1:1212412/1‑151 (MQ=255)
                                                                                                cgcgcATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGAt                                                  <  2:1223932/150‑1 (MQ=255)
                                                                                                cgcgcATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGAt                                                  <  1:1320426/150‑1 (MQ=255)
                                                                                                cgcgcATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGAt                                                  <  2:547051/150‑1 (MQ=255)
                                                                                                  cgcATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAAc                                               <  2:1352249/151‑1 (MQ=255)
                                                                                                  cgcATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAAc                                               <  2:1123025/151‑1 (MQ=255)
                                                                                                  cgcATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAAc                                               <  1:1610119/151‑1 (MQ=255)
                                                                                                  cgcATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAAc                                               <  2:67968/151‑1 (MQ=255)
                                                                                                  cgcATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAATAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAAc                                               <  2:955987/151‑1 (MQ=255)
                                                                                                    cATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAAcc                                              <  2:1440521/150‑1 (MQ=255)
                                                                                                    cATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCt                                             <  2:255532/151‑1 (MQ=255)
                                                                                                          gCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGCAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGc                                          <  2:744217/148‑1 (MQ=255)
                                                                                                               tttCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGCAGCCCCaa                                  >  2:237519/1‑151 (MQ=255)
                                                                                                               tttCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGCAGCCCCaa                                  >  2:1535533/1‑151 (MQ=255)
                                                                                                               tttCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGCAGCCCCaa                                  >  2:196312/1‑151 (MQ=255)
                                                                                                                 tCTGCGGTTAGCGCCGAA‑CAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGCAGCCCCAacac                               <  2:539707/151‑1 (MQ=255)
                                                                                                                  cTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCCCCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATTCCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGCAGCCCCAacac                               <  2:1262823/151‑1 (MQ=255)
                                                                                                                  cTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGCAGTCCCAacac                               <  2:1194337/151‑1 (MQ=255)
                                                                                                                  cTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGCAGCCCCAacac                               <  1:506346/151‑1 (MQ=255)
                                                                                                                     cGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGCAGCCCCAACACCAt                            <  2:1135739/151‑1 (MQ=255)
                                                                                                                        ttAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGCAGCCCCAACACCATTcc                         <  2:835244/151‑1 (MQ=255)
                                                                                                                        ttAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGCAGCCCCAACACCATTc                          <  2:219066/150‑1 (MQ=255)
                                                                                                                        ttAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGCAGCCCCAACACCATTc                          <  2:1506006/150‑1 (MQ=255)
                                                                                                                            cgcCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGCAGCCCCAACACCATTCCGa                       <  1:508722/149‑1 (MQ=255)
                                                                                                                            cgcCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGCAGCCCCAACACCATTCCGa                       <  1:697895/149‑1 (MQ=255)
                                                                                                                               cGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGCAGCCCCAACACCATTCCGATGAac                  <  1:1302588/151‑1 (MQ=255)
                                                                                                                                         cGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGCAGCCCCAACACCATTCCGATGAACACGCCAGaaa        <  2:1512766/151‑1 (MQ=255)
                                                                                                                                           cccGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGCAGCCCCAACACCATTCCGATGAACACGCCAGAAAcc      <  1:757128/151‑1 (MQ=255)
                                                                                                                                              ggCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGCAGCCCCAACACCATTCCGATGAACACGCCAGAAACcaca   >  1:682617/1‑151 (MQ=255)
                                                                                                                                               gCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGCAGCCCCAACACCATTCCGATGAACACGCCAGAAACCaaaa  >  1:1288541/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GTGGTAATGAAATAACCCCAGCCCAGAAACGGGGAGAAATAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGCAGCCCCAACACCATTCCGATGAACACGCCAGAAACCACAA  >  NC_000913/3338780‑3339073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑