Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_LN832404	3,419,910	C→T	9.8%	pseudogene (74/81 nt)	EK12AG100_RS25785 →	paraslipin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_LN832404	3,419,910	0	C	T	9.8%	188.0 / 11.5	71	pseudogene (74/81 nt)	EK12AG100_RS25785	paraslipin
Reads supporting (aligned to +/- strand):  ref base C (30/34);  new base T (2/5);  total (32/39)
Fisher's exact test for biased strand distribution p-value = 4.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-01

GAAATAGTGGAGCAAGCTGCACCTGATGAATTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCG  >  NZ_LN832404/3419617‑3420203                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                       gAAATCGTGGAGCAAGTGGCACCTGATGAATTTTTTTGGCATCCTTAATCAGGGCTTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATTTTATTGCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTcc                                                                                                                                                                                                                                                                                                       <  2:273227/295‑1 (MQ=21) gAAATAGTGGAGCAAGCTGCACCTGATGAATTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCCCCCTCCGGGCCGTTGCTTCGCACCGTTCAAATCCCCCCCCGGCGCGTTTGTCCTTCCCCGGAGCGTGTTCCCCCTCCAACCACCGACAAAACCAAAGTCCCCGtcctcc                                                                                                                                                                                                                                                                                                       >  2:91161/1‑295 (MQ=255)     tattGGATCAAGGTGATCCTGATGAATGTTTGGCGGAGCCTACATCTGAGCGTACGAGGGCATTTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTGGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGAc                                                                                                                                                                                                                                                                                                    <  2:425807/292‑1 (MQ=21)     tAGTGGAGCAAGCTGCACCTGATGAATTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACt                                                                                                                                                                                                                                                                                                   <  1:683150/295‑1 (MQ=255)         gttcaggcagttcaatcaactgtttaccttgtggagcgacatccagaggcacttcaccgcttgccagcggcttaccatccagcgccaccatccagtgcaggagctccgatgataagctgtcaaacatgagaattccgatccccaattcCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGa                                                                                                                                                                                                                                                                                                 <  1:753732‑M2/147‑1 (MQ=255)               gcagttcaatcaactgtttaccttgtggagcgacatccagaggcacttcaccgcttgccagcggcttaccatccagcgccaccatccagtgcaggagctccgatgataagctgtcaaacatgagaattccgatccccaattcCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTAACCGACCAACAAACGGTAAAACGAAAGGCCCAGTCTTTCGACTGAGCCatt                                                                                                                                                                                                                                                                                           >  1:333934‑M2/143‑295 (MQ=255)                  gttcaatcaactgtttaccttgtggagcgacatccagaggcacttcaccgcttgccagcggcttaccatccagcgccaccatccagtgcaggagctccgatgataagctgtcaaacatgagaattccgatccccaattcCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGt                                                                                                                                                                                                                                                                                        >  1:315417‑M2/140‑295 (MQ=255)                     caatctaccgctttccttgttgctcgtcagctagcggcacgtcaccgcttgtcagcggcttacaatccggcgccaccatccagtgcaggagctccgatgctaagctggcaaacatgagaattccgatccccaattcCTGGCAGTGTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGTGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTTTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGtttt                                                                                                                                                                                                                                                                                     <  2:749760‑M2/159‑1 (MQ=255)                       atcaactgtttcccttgtggagcgacatccagaggcccttcaccgcttgccagcggcttaccatccagcgccaccatccagtgcaggagctccgatgataagctgtcaaacatgagaattccgatccccaattcCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTAt                                                                                                                                                                                                                                                                                   <  1:293050‑M2/161‑1 (MQ=255)                        ttatgaATTTTTTGCGCATCCTAAATCATGGCGTACGAGGGCA‑TTTTTATCGCAGTTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTg                                                                                                                                                                                                                                                                                <  1:125322/293‑1 (MQ=255)                         gatgaATTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGa                                                                                                                                                                                                                                                                               >  1:294844/1‑295 (MQ=255)                              gtttaccttgtggagcgacatccagaggcacttcaccgcttgccagcggcttaccatccagcgccaccatccagtgcaggagctccgatgataagctgtcaaacatgagaattccgatccccaattcCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGAtgc                                                                                                                                                                                                                                                                            <  1:129515‑M2/168‑2 (MQ=255)                              aTTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCt                                                                                                                                                                                                                                                                          >  1:410132/1‑295 (MQ=255)                                   ccttgtggagcgacatccagaggcacttcaccgcttgccagcggcttaccatccagcgccaccatccagtgcaggagctccgatgataagctgtcaaacatgagaattccgatccccaattcCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCtggg                                                                                                                                                                                                                                                                       >  1:135069‑M2/123‑294 (MQ=255)                                    cttgtggagcgacatccagaggcacttcaccgcttgccagcggcttaccatccagcgccaccatccagtgcaggagctccgatgataagctggcaaacatgagaattccgatccccaattcCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCctgggg                                                                                                                                                                                                                                                                      <  2:86226‑M2/174‑3 (MQ=255)                                     gcgcATCCTAAATCAGAGCGTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAACGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGtt                                                                                                                                                                                                                                                                   >  1:587636/1‑295 (MQ=255)                                          tCCTAAATAAGGGCGTCCGAGGCCA‑TTGTTAGCCAAGGTAATCCATTATGTAAATTGTAGTTCGAAAAGAAAAAAGGCCT‑TCTTGCCGGTTGGCGTTTCGCTTGATTTGATGTCTGGCAGTTCATGGCGGGC‑GTCCGGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTa                                                                                                                                                                                                                                                              <  2:271673/295‑1 (MQ=17)                                              gacatccagaggcacttcaccgcttgccagcggcttaccatccagcgccaccatccagtgcaggagctccgatgataagctgtcaaacatgagaattccgatccccaattcCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCCGCGGATTTGTCCTACTCCTGAGAGCGTTCACCGACAAACAACAGCTAAAACGAAACGCCCAAGCTCTCGTATAACCCTTTCGTTTtattttatgcctgggggatttatttc                                                                                                                                                                                                                                                            >  2:162709‑M2/112‑283 (MQ=255)                                                   agagCGTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCCCCCGGCGGATTTGTCCTACCCGGGAGAGTGTTCACCGACAAACCACAGGTAAAACAAAAAGCCCAGTCTTCCGGACGAACCTTTTGGTTTATTTGATTGCTGGCCTTTACCTtctccccgct                                                                                                                                                                                                                                                     >  2:276315/1‑289 (MQ=18)                                                         tACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCCCCCTCCGGGCCGTTGCTTCGCCACGTTCCAATCCCCTCCCGGCGGATTTGGCCTACTCGGGAGAGTGTTCACCGACCACCAACCCATAAAACAAAAGTCCCAACCTTCCGACTGCGCCTTTTGCTTTATTTTATGTCTAGCAGTTCCCTTCTCTCCCCTGGGgac                                                                                                                                                                                                                                               >  2:243085/1‑294 (MQ=255)                                                         tACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTCCTCTCGCATGGGgag                                                                                                                                                                                                                                               >  1:404774/1‑295 (MQ=255)                                                         tACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATggggcg                                                                                                                                                                                                                                               >  1:546715/1‑293 (MQ=255)                                                                      tttAACGCAGTGAATCCATAAATTGAATGTTCTGTCGAAAAGCAAAATGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGAGTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTAc                                                                                                                                                                                                                                  <  2:241987/295‑1 (MQ=255)                                                                          tCGCAGGTAATCCATTAATTGAATGTTATTTCGAAAGGCAAAACGGCCT‑TCCTTTCGGATGGCCTTTCGCTTGATTTTATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATc                                                                                                                                                                                                                              <  1:63530/295‑1 (MQ=255)                                                                                   aTCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACC‑ATACCATCGGCGCTACg                                                                                                                                                                                                                     >  1:701122/1‑295 (MQ=255)                                                                                     caaTTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCTTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGc                                                                                                                                                                                                                   <  1:685384/293‑1 (MQ=255)                                                                                         tAATTGAAAGTTAGTTCAAAAAGAAAAAAGGGCT‑GGCTTTCGGATGGTCTTTGGCTTGATTTGCTTGTTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCGCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGttt                                                                                                                                                                                                               <  2:427046/295‑1 (MQ=14)                                                                                            ttGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTAACCACGGCGCTACGGCGGTTCAc                                                                                                                                                                                                            >  1:186248/1‑295 (MQ=255)                                                                                                    cggTTAGAAAAGAAAAAAGGCAC‑TCCTTTCGGTGGGCCTTTGGCTTGATTTGATGTCTGGCAGTGTACGGCGGGC‑GTCCTGCCCGCCACCCGCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGt                                                                                                                                                                                                    <  2:8479/293‑1 (MQ=14)                                                                                                     aGTTCGACAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGtt                                                                                                                                                                                                   >  1:447743/1‑295 (MQ=255)                                                                                                     aGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCCCTTCTGAGtt                                                                                                                                                                                                   >  1:692308/1‑295 (MQ=255)                                                                                                       gtCGCAAAGCCAAAAGGGCACTCCTTTGTGGTGGCTTTGTGCGTGATTTGATGTCGGGCGGGTTATGCCGGGC‑GTCCTGCCCGCAACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTc                                                                                                                                                                                                  <  2:547051/294‑1 (MQ=14)                                                                                                                    aaGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTTTGGCAGTTCCCTACTCTCGCCAGGTGACACCCCACC‑ATACCCTCGGCACCACGGCCATTTCCTTCTGTGTTCGGctacgggccagg                                                                                                                                                                                    >  2:214584/1‑284 (MQ=14)                                                                                                                        gcctgccTTTCGGCGGGCCTTTCGCTGGATTGGATGGCGGGGAGTGTATTGCGGGC‑GGCCTGCCCGCCACCCGCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTgg                                                                                                                                                                                 <  2:699705/290‑1 (MQ=14)                                                                                                                          a‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCCGGAGGGTTTTCCCCGACAAACCACAGATAAAACCAAAGGCCCAATCTTCCGACCGAGCCTTTTGTTTTTTTTGATGTCTGGCAGTTCCCTACCCTCTCATGGGG‑GTCCCCCCACCTACCATCGGCCCTACCGCCGTTCACTTCTGGCTTTGGCCTCGGGTCAGGTgggtc                                                                                                                                                                              >  2:153646/1‑293 (MQ=14)                                                                                                                          a‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGAGCCGTTGCTTCGCAACGTTCCAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTCTTTGATGTCTGGCAGTTCCCTACTCTCGCCTGGGGAGGCCCCACC‑CTACCACCCGCCCTACCGGCTTTCCCTTCTTGTTTAGCCCTTGGGTCCGGTgggtc                                                                                                                                                                              >  2:88110/1‑293 (MQ=14)                                                                                                                             ccTTTCGGATGGCCTTTCGCTTGATTTGATGTCGGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTCTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGacca                                                                                                                                                                            <  1:579000/295‑1 (MQ=255)                                                                                                                               tttCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGaccacc                                                                                                                                                                          >  1:279436/1‑295 (MQ=255)                                                                                                                                  cGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCCGGTCCCTACCCTCGCATGGGCATCCCCCACA‑CTAACCTCCGCCCTACGGCGCTTCCCTTCTTCGTTCCGCCCGGGGACCGGTggccgcaccggc                                                                                                                                                                       >  2:304641/1‑285 (MQ=14)                                                                                                                                     aTGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACCCGGGAGAGTGTTCACCGACAAACCACCGATAAAACAAAAGGCCCAGTCTTCCGACCGAACCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCCCATGGGGAGACCCCACC‑CTACCCACCGCGCTACGGCGGTTCCCTTCTTGCTTCGGCCTTGGGCCCAGTGGGACCACCGCGCtc                                                                                                                                                                    >  2:534826/1‑294 (MQ=14)                                                                                                                                               cctggttttgatGTCGGGCCGTTT‑TGGTGCGCGGCCCTGCCCGCCACCCTCCGTGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTAACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAg                                                                                                                                                          <  2:607689/289‑1 (MQ=11)                                                                                                                                                cTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCCTGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGa                                                                                                                                                         >  1:331319/1‑295 (MQ=39)                                                                                                                                                 ttgatttgatGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACC‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTTGGCATGGGGTCAGGTGGGACCACCGCCGACCTGCCGCCAgcg                                                                                                                                                        >  2:52161/1‑293 (MQ=255)                                                                                                                                                   gatttgatGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACaa                                                                                                                                                      <  1:52161/295‑1 (MQ=39)                                                                                                                                                            tCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCCTGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTc                                                                                                                                             >  1:701685/1‑295 (MQ=39)                                                                                                                                                             cTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCt                                                                                                                                            <  1:721909/295‑1 (MQ=39)                                                                                                                                                                        ttgcgtgc‑gtCCTTCCCGCCGCCCTCCTGGCCGTTGCTTCGCCACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGaa                                                                                                                                 <  1:292071/293‑1 (MQ=255)                                                                                                                                                                           cggtc‑gTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACGCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACtt                                                                                                                              <  1:180857/295‑1 (MQ=255)                                                                                                                                                                             ggc‑gTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTa                                                                                                                            <  1:534826/295‑1 (MQ=255)                                                                                                                                                                                  tCCTGCCCGCCACCCTCCCGGCCGTTGCTTCTCAACGTTCAAATCCGCTCCCTGCGGATTTGTCCTACGCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGTCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCt                                                                                                                        <  1:436220/295‑1 (MQ=255)                                                                                                                                                                                          gCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAgtg                                                                                                                >  1:326789/1‑295 (MQ=255)                                                                                                                                                                                             ccccTCCGGGCCTTTGCCTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAgtggtg                                                                                                             <  2:385103/294‑1 (MQ=255)                                                                                                                                                                                                   ccggCCGTGCCCTCGCAACGTTCAAATCCGCTCCCGGCGGATTGTCCCTCATCGGGATAGTGTTCACCGACAAACAACAGATAAACCAAAAGGCCCAGTCGTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATa                                                                                                       <  2:186248/293‑1 (MQ=17)                                                                                                                                                                                                          ttGCTTCGCAACGTTCACATCCGCTCCCGGCGGATTTGTCCTACACGGGCGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGTGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTCCTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCagag                                                                                                <  2:294844/295‑1 (MQ=255)                                                                                                                                                                                                          ttGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCagag                                                                                                <  1:8127/295‑1 (MQ=255)                                                                                                                                                                                                             cTTCGCAACGTTCAAATCAGCGCCCGTCGGATTTGTGCTACTCGGGAGATTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCg                                                                                             <  2:492054/295‑1 (MQ=255)                                                                                                                                                                                                              ttCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGa                                                                                            <  1:276315/295‑1 (MQ=255)                                                                                                                                                                                                                cGCAACGTTAAAATCCGCTCCCGGCGGATGTGTCCTACGTGGGAGAGTGTTCACCGACAAACAACAGAGAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGGTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAAc                                                                                          <  2:701685/295‑1 (MQ=255)                                                                                                                                                                                                                          aaaCCCGCTCCCGGCGGATTGTTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGGTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTc                                                                                <  2:69806/295‑1 (MQ=255)                                                                                                                                                                                                                            aTCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAATGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCAc                                                                              >  1:532632/1‑295 (MQ=255)                                                                                                                                                                                                                            aTCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAACGTGGTTCTGCTACCCAGAGTCGAGCTGGGGACCtacc                                                                              >  1:317479/1‑292 (MQ=255)                                                                                                                                                                                                                             tCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACCAATTCTTTTCTCATCTGCCGAACTTTAACCTAAAACGTGGTGCTGAGTCCCAGAGTCGGCCTGGGGAccccccc                                                                             >  2:288965/1‑290 (MQ=255)                                                                                                                                                                                                                                 cTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTACAAAGTGGTGCTGATACCCAAAGTCGAACTGGGGAACtacccactt                                                                         >  2:694088/1‑287 (MQ=255)                                                                                                                                                                                                                                     cggcggATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCCTGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAACAGTGGTGCTGATACCCAGAGTAGAACTGGGGCCCTCACCCTTACCaa                                                                     >  2:728836/1‑295 (MQ=255)                                                                                                                                                                                                                                     cggcggATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGACGAACTGGGGACCTCACCCTTACaaa                                                                     >  1:550088/1‑295 (MQ=255)                                                                                                                                                                                                                                        cggATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAggg                                                                  >  1:66107/1‑295 (MQ=255)                                                                                                                                                                                                                                         ggATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTACAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACTCTTACCACgggg                                                                 >  2:376360/1‑294 (MQ=255)                                                                                                                                                                                                                                                       cGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACt                                                   <  1:288965/295‑1 (MQ=255)                                                                                                                                                                                                                                                              gtgtTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCat                                            <  1:78277/295‑1 (MQ=255)                                                                                                                                                                                                                                                                gtTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACATAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCatat                                          >  1:307647/1‑295 (MQ=255)                                                                                                                                                                                                                                                                      cGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCAc                                    <  1:728836/295‑1 (MQ=255)                                                                                                                                                                                                                                                                       gACTAACAACAGATAAAACCAAAGGCCCCGGCTTCCGGCTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTGCTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACg                                   <  2:326789/295‑1 (MQ=255)                                                                                                                                                                                                                                                                        acaaacaaCAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAATTTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCCAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCACCTGAGCCATATCAGCCCGc                                  >  1:10199/1‑295 (MQ=255)                                                                                                                                                                                                                                                                          aaacaaCAGATAAAACAAAAGGCCCATTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTa                                <  1:376360/295‑1 (MQ=255)                                                                                                                                                                                                                                                                                      aaaCAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCt                    <  1:774708/295‑1 (MQ=255)                                                                                                                                                                                                                                                                                          aaaaGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCa                <  1:153646/295‑1 (MQ=255)                                                                                                                                                                                                                                                                                             aGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGACCTTTAACCTAACAAGTGTTTCTGATACCCAGAGTCGAACTGGGGACCTCACACTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTACATTTGATGCCTGGCAGtt             >  1:225960/1‑295 (MQ=255)                                                                                                                                                                                                                                                                                             aGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGtt             <  1:694088/295‑1 (MQ=255)                                                                                                                                                                                                                                                                                                cccAGTCTTCTGACTGTGCCTTTTGGTTGATTTTATGTCCTGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTccc          <  2:701122/295‑1 (MQ=255)                                                                                                                                                                                                                                                                                                        tCCGACTTGGTCTTTTGTGTTATTGTAGTGCTGGCAGTTCCCTACTCTCGCATGGGGATACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGTCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCg  <  2:447743/295‑1 (MQ=37)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                       GAAATAGTGGAGCAAGCTGCACCTGATGAATTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGCA‑TTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCA‑TCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACA‑CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCG  >  NZ_LN832404/3419617‑3420203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑