Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_LN832404 4,303,525 T→C 86.8% N250D (AAT→GAT)  alsK ← allose kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_LN8324044,303,5250TC86.8% 194.7 / 18.3 75N250D (AAT→GAT) alsKallose kinase
Reads supporting (aligned to +/- strand):  ref base T (6/4);  new base C (29/35);  total (36/39)
Fisher's exact test for biased strand distribution p-value = 5.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

TCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATTCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTCAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGT  >  NZ_LN832404/4303243‑4303802
                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                      
tatttGCAGAAAATAATATTAGTCGTTTTATGTGTTTATTAGATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCAAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCAAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCAcc                                                                                                                                                                                                                                                                            <  2:92394/292‑1 (MQ=255)
tCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCAcc                                                                                                                                                                                                                                                                            >  1:133312/1‑295 (MQ=255)
   tggaaGAAATTAAAGTGAGTCGTTTTTGTTGTTTATTATTTATAGTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTGTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGcc                                                                                                                                                                                                                                                                         <  2:429966/291‑1 (MQ=255)
     gCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCa                                                                                                                                                                                                                                                                       <  1:79474/295‑1 (MQ=255)
          aaaTAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGGGGACGGCGCAGGTACTTTGGGGTCATGGCAACGAGAGTCCCGCGGGGGAAGGGGGACATATCCAACACACCACCGCCCAGGacc                                                                                                                                                                                                                                                                  >  2:271882/1‑293 (MQ=255)
           aataatATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCCTATTCATCACGCCACCGCCCAGGATcc                                                                                                                                                                                                                                                                 >  1:103388/1‑294 (MQ=255)
           aataatATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCCGGATcc                                                                                                                                                                                                                                                                 >  1:667868/1‑294 (MQ=255)
           aataatATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCCAGCGGGGGAGGGCGGGGATATTCATCACGCCACCGCCGCGGAtgc                                                                                                                                                                                                                                                                 >  2:348098/1‑293 (MQ=255)
               atatGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCCTAACTCCACGGCCCAGGCGCACCGc                                                                                                                                                                                                                                                             >  2:583888/1‑295 (MQ=255)
                   gAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGGGGGAAGGCGGGCATATCCATCCCGCCACCGCCCAGGATCCCCggctcg                                                                                                                                                                                                                                                         >  2:240503/1‑290 (MQ=255)
                        gTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATc                                                                                                                                                                                                                                                    <  1:622293/295‑1 (MQ=255)
                                   tttttatATAGATTGTACCTTTCGTTGGCACAGCGGGACATTGAGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTaa                                                                                                                                                                                                                                         <  2:78241/292‑1 (MQ=255)
                                          atatatTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGCACAGATTAATGCtggt                                                                                                                                                                                                                                  >  1:386284/1‑295 (MQ=255)
                                             gtctgtgCCGGTTGTTGGCCCTGCGCGACAATGCGTTCCTTGTCCAAGTGTTTACTATTAGGGCTTTAGCACAGAACTTTGGCAAAAAACGTTGATGCGCCAATATGGCTGCACCCTGAGCGCCATTAAAGTCAGAAGAGTAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGc                                                                                                                                                                                                                               <  2:437181/292‑1 (MQ=255)
                                                    ccTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATACATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGcc                                                                                                                                                                                                                        >  1:682539/1‑295 (MQ=255)
                                                       gTCGTTGACACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATTCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGt                                                                                                                                                                                                                     >  1:454502/1‑295 (MQ=255)
                                                          gTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGGACAGATTAATGCTGGTGGCAATGGCCCGTGcc                                                                                                                                                                                                                  >  1:435120/1‑295 (MQ=255)
                                                          gTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGcc                                                                                                                                                                                                                  >  1:673767/1‑295 (MQ=255)
                                                           ttGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGCGAGTCCAGCGTGGGAAGGGGGGCATACACATCACGCCACCGCCCAAGATCACCGCATCAGGAACGCACAACATAACGCAGGCGGCAATGGcacaggacg                                                                                                                                                                                                                 >  2:739264/1‑287 (MQ=255)
                                                            gggCACAGCGGGAAATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAGTATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCcgc                                                                                                                                                                                                                <  1:235941/294‑1 (MQ=255)
                                                             ggaacaGCGGGACAGTGCGCTAATGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTGGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGAACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCcgcg                                                                                                                                                                                                               <  1:493344/292‑1 (MQ=255)
                                                                acaGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATTCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGttt                                                                                                                                                                                                            >  1:358621/1‑295 (MQ=255)
                                                                  aGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGAACTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCAACGCATCGGGAACGAAACGATTAATGCTGGTGGAAATGGCCCGGGCCGCGTTTTc                                                                                                                                                                                                          >  1:36048/1‑295 (MQ=255)
                                                                             gggctatggtgCCAACGTTTTACCCAGGGGGCGTTAGCACAGAACCGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGActct                                                                                                                                                                                               <  2:539466/287‑1 (MQ=255)
                                                                              cgcTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTg                                                                                                                                                                                              <  1:424854/295‑1 (MQ=255)
                                                                                 tACTGTGCCACCGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGAc                                                                                                                                                                                           <  1:328621/295‑1 (MQ=255)
                                                                                               tttACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGTGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGtttt                                                                                                                                                                             <  1:49160/295‑1 (MQ=255)
                                                                                                     cATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCAt                                                                                                                                                                       <  1:290075/295‑1 (MQ=255)
                                                                                                                 gCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAGTGCTGGTTGCAATGGCCCGTGCCGCGTTTTCTAGGCGACTCGCGCCGAAAGGGGCGTTTTCCCCATGGGAGAAAAGag                                                                                                                                                           >  2:374599/1‑294 (MQ=255)
                                                                                                                  cacaGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATc                                                                                                                                                          >  1:251292/1‑295 (MQ=255)
                                                                                                                  cacaGAACTGTGGAAAAAAACGTTGATGCGCCAATAGTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATc                                                                                                                                                          <  1:595234/295‑1 (MQ=255)
                                                                                                                   acaGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTCTCCAAGCAGACTCTGGACGAAAGGTGCGTTTTCCGCATGGCCGAAAAGAGcg                                                                                                                                                         >  1:413052/1‑295 (MQ=255)
                                                                                                                    caGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATTCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATcgc                                                                                                                                                        <  1:348098/295‑1 (MQ=255)
                                                                                                                     aGAACTGTGGCAAAAAACGTTGAGGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATcgcg                                                                                                                                                       <  1:271882/295‑1 (MQ=255)
                                                                                                                          tgtgGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGAGCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGACGACTAGGGACGAACGGGGCGTTTTCCGCATGGACGAAAAGAGCGCGCacgg                                                                                                                                                  >  2:109375/1‑292 (MQ=255)
                                                                                                                             ggCAAAAAACGGTAGTGCGCCAATGTGGCGGCACCCTGAGCGCAATGAAAGGAAGAAGAGGAGGCGGCAATAAAGCGCACGACCGGATGCGGCAGTGGACGGCGAAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGt                                                                                                                                               <  2:234866/295‑1 (MQ=255)
                                                                                                                              gCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATTCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTa                                                                                                                                              <  1:175736/295‑1 (MQ=255)
                                                                                                                                  aaaaCGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAAttt                                                                                                                                          <  1:583888/295‑1 (MQ=255)
                                                                                                                                   aaagtgtaaTGTGCCAAGATTGCTGAACCCTGAGCTCCAGTAAAGTCAGAAGAGGAGGCGGCAATAAAGCGAACGACCTGATGCGGCAGGGGACGGCGCAGGTACTGTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCACAGGATCACCGAATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTc                                                                                                                                         <  2:533852/287‑1 (MQ=255)
                                                                                                                                     aCGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCgg                                                                                                                                       >  1:30889/1‑295 (MQ=255)
                                                                                                                                            tGCGCCAATATTGCTGCACCCGGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATTCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCtgtt                                                                                                                                <  1:280951/295‑1 (MQ=255)
                                                                                                                                             gcccccaacgtcctgcACCCTCGGCGCCATTCAGCGCTGAATGTGACACGGCAATAAAGCTCACGACCTGATTCGGCAGTGGACGTCGCAGGTACTTTTGGGTCATGGCCACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCAGCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCtgttg                                                                                                                               <  2:36048/283‑1 (MQ=255)
                                                                                                                                               gcCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCGTCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTgttgtt                                                                                                                             >  1:154645/1‑295 (MQ=255)
                                                                                                                                               gcCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGTCTgttgtt                                                                                                                             >  1:111914/1‑295 (MQ=255)
                                                                                                                                                ccAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTc                                                                                                                            <  1:771814/295‑1 (MQ=255)
                                                                                                                                                   atgttGCGGCACCCGAAGGGCCATTAAAGTCGTAAGAGGAGGCGGCAATAAAGCGGAGGACCTGGTGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTa                                                                                                                         <  2:637426/292‑1 (MQ=255)
                                                                                                                                                    tatTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTAc                                                                                                                        <  1:176002/295‑1 (MQ=255)
                                                                                                                                                             agccTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGtctt                                                                                                               <  2:588145/293‑2 (MQ=255)
                                                                                                                                                                   aGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGcc                                                                                                         >  1:443287/1‑295 (MQ=255)
                                                                                                                                                                   aGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGGCTTAGCGcc                                                                                                         >  1:658278/1‑295 (MQ=255)
                                                                                                                                                                   aGCGACGTTAAAGTCAGAAGATGGTGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGcc                                                                                                         <  2:586768/295‑1 (MQ=255)
                                                                                                                                                                      gcCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGGGCCGCGGTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGAAGAAAAGGACGCGCAATGGGTGATTTCGGGGCTGGTGTTCGTACCAGGGTCGTAGCGCCAtt                                                                                                      >  2:293903/1‑295 (MQ=255)
                                                                                                                                                                           gaaaGGCAGAAGATGAGGGGGCAATAAAGGGCAGGACCTGATGCGGCAGTGGACGGAGCAGGGACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCaga                                                                                                 <  2:598149/294‑1 (MQ=255)
                                                                                                                                                                                tCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAAt                                                                                            >  1:743279/1‑295 (MQ=255)
                                                                                                                                                                                tCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGGAAGTTCGGGGCTGGTTTTCGTACCAGCGTCTTAGCGCCATTCCaggccaaa                                                                                            >  2:139773/1‑289 (MQ=255)
                                                                                                                                                                                   gaagaTGGGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTgg                                                                                         <  1:146077/295‑1 (MQ=255)
                                                                                                                                                                                    aagaTGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAAGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGt                                                                                        <  1:739264/295‑1 (MQ=255)
                                                                                                                                                                                     agaTGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGtt                                                                                       >  1:221630/1‑295 (MQ=255)
                                                                                                                                                                                         gAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGAAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGCCGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCa                                                                                   >  1:721203/1‑295 (MQ=255)
                                                                                                                                                                                                  aTAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATTCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGGCCGTGCCGAGTTTTCAAGCAGACTCTGGACGAAAGGGGAGGTTTCCGCATGGCCGAAAAACTAGCGCACTGGGTAATTTCGGGGCTGTTGTTTGTACCAGCGTCCCACAGCCCTCCCAGAGCCACTGGTTTCACGGCCCCCac                                                                          >  2:251592/1‑294 (MQ=255)
                                                                                                                                                                                                         gcACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCGTCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGcc                                                                   <  2:154645/295‑1 (MQ=255)
                                                                                                                                                                                                          cACGACCTGATGCGGCAGTGGACGGCGCATTTCCTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCca                                                                  >  1:693483/1‑295 (MQ=255)
                                                                                                                                                                                                              aCCTGATGCTGCAGGGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACAcg                                                              <  1:637747/295‑1 (MQ=255)
                                                                                                                                                                                                              aCCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACAcg                                                              <  1:293903/295‑1 (MQ=255)
                                                                                                                                                                                                                       ggCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGAACGCGCAAGTGTTAATTTCGGGGATGTTGTTCGTACCCGCGTTCTAGCGCCCATCACGGATAATTGGTTTCCAGGACCCCAGGGTGCCCCCACTCGCAgcgta                                                    >  2:555736/1‑294 (MQ=255)
                                                                                                                                                                                                                          aGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATTCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTCAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCAACCGCGCAGTGTTggg                                                 >  1:36632/1‑295 (MQ=255)
                                                                                                                                                                                                                             ggACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGACATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCACAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGCGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTTTTGGGTCa                                              >  1:627762/1‑295 (MQ=255)
                                                                                                                                                                                                                                 gtcgcAGGGACTTTTGGGTCATGGCAACGGGAGTCTGGGGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATAtc                                          <  2:71941/293‑1 (MQ=255)
                                                                                                                                                                                                                                 ggCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATTCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGTGGCTGTTGTTCGTACCAGCGGCTCAGCGCCATTCCAGAGCAATTGGTTTCCGGGCCCCCAGGAGTTCCCCCAGCGCAAGTGTTGGTCatatg                                          >  2:396477/1‑294 (MQ=255)
                                                                                                                                                                                                                                    gcAGGGACTGTTGGGTGCTGGCAACGAGGGGCTCGCGTGGGAAGGCGGGCACATTCAGCACGCCACCGCCCAGGATAACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTCAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTcc                                       <  2:358621/295‑1 (MQ=255)
                                                                                                                                                                                                                                     ctggTACTTTTGGGGCATGGCAACGGGGGTCTAGGGTGGGAAGGCGGGAATATCCATGCCGCCACCGCCCAGGATCACCGCATCGGGATCGAACGGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTccc                                      <  2:351206/293‑1 (MQ=255)
                                                                                                                                                                                                                                      aGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCa                                     >  1:90648/1‑295 (MQ=255)
                                                                                                                                                                                                                                             tttGGGTAATGGCAACGAGAGTCTCACGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAAAAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGat                              <  1:91169/295‑1 (MQ=255)
                                                                                                                                                                                                                                                gggTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATg                           <  1:139773/295‑1 (MQ=255)
                                                                                                                                                                                                                                                 ggTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTGATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGa                          <  1:240503/295‑1 (MQ=255)
                                                                                                                                                                                                                                                      ggggaaCGTGAGTCTGGCGTGGGAAGGCGGGCAGATCCATCACGCAACCGCCCAGGATCACCTCGTCGGGAGCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAg                     <  2:768881/291‑1 (MQ=255)
                                                                                                                                                                                                                                                        gCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGtt                   >  1:294717/1‑295 (MQ=255)
                                                                                                                                                                                                                                                          aaCGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTAACAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCAACACGCGAAGTGTTGGGACATACACCCCAGGGGGATATTACCCAGTTct                 >  2:170279/1‑294 (MQ=255)
                                                                                                                                                                                                                                                           aCGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCCCAGTGTTGGGTCATATCTCCAAGGGGGATATTACCCAGTTCGc                >  1:705838/1‑295 (MQ=255)
                                                                                                                                                                                                                                                                     cgcgTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACAcc      <  1:555736/295‑1 (MQ=255)
                                                                                                                                                                                                                                                                      gcgTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCCATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTGAGCGCCATTCCAGCGCCATTGGTTTCCCGGAACACCGGCATCCCACACGCGCATTGCTAGGTCATCACTCCCAACGGGATGTGACACAGCTccacccacaccacc     >  2:588100/1‑282 (MQ=255)
                                                                                                                                                                                                                                                                       cgTGGGAAGGCGGGCCTATGCTGCACGCCACCGCCCAGGGTCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTCAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCgt    <  2:36632/295‑1 (MQ=255)
                                                                                                                                                                                                                                                                         tGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACCCCgtgt  >  1:76007/1‑295 (MQ=255)
                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                      
TCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATTCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTCAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGT  >  NZ_LN832404/4303243‑4303802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: