| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NZ_LN832404 | 3,724,623:1 | +C | 100% | coding (1261/1353 nt) | xylB ← | xylulokinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_LN832404 | 3,724,623 | 1 | . | C | 96.1% | 358.2 / 8.0 | 103 | coding (1261/1353 nt) | xylB | xylulokinase |
| Reads supporting (aligned to +/- strand): ref base . (2/2); new base C (40/58); total (43/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
Only 100 of 122 total aligned reads displayed.
AACAAATGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑T‑CCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAA > NZ_LN832404/3724331‑3724898 | aaCAAATGATTTTCGGAATAAATTCTAACTAAATTATAACTACTGACTGAGTTAGATCGATGTGACTTATCCCCCACCCGGTCAGGCAGGGGATAACGTTAACGCAATTAATGGCAGAAGTTGCTGATGGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑T‑cc < 2:396736/295‑1 (MQ=255)aaCAAATGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCCACAATTCAATGAGCGA‑TTTCTCTGGATTCGCGGCGACATGCGCCAGCCTTCCTGACCCCAGTGCTGCCCCCAC‑A‑c‑cc > 2:657375/1‑295 (MQ=255) aTTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCTCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑T‑cccc‑c‑cccgt > 1:711482/1‑292 (MQ=255) ttttCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGTGAATTATCCCCCGCCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑T‑cccc‑c‑cccgta > 1:822754/1‑291 (MQ=255) ttttCAGAATAAAATCATACTAAATATTAATTAATTACTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAACGA‑TTTCTCTGGATTCGCCGCGATCTGCCCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑T‑cccc‑c‑cccgta < 1:765203/295‑5 (MQ=255) cAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGCTAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCCACAATTCAATGAGCGC‑TTTCTCTGGTTTCGCCGCGTTCTGCGCCAGCCTAGCTGCGCCCAGTTCTGGACCCAC‑C‑A‑cccc‑c‑cccacacggt > 2:540114/1‑287 (MQ=255) aGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑T‑cccc > 1:238106/1‑283 (MQ=255) aGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑T‑cccc < 2:238106/283‑1 (MQ=255) attcTAAATATTAATTAATTACTGAGATATATAGAGGTGAATTCTCCCCCACCCGGTCTGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTAGTGGCTGATAAGCGGCTTAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAgctgct < 1:684704/292‑1 (MQ=255) agaCTAAATATTAATTAATTACTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAgctgct < 1:729089/293‑1 (MQ=255) tACTAAATATTAATTAATTACTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGGAATCGAGctgctg > 1:525385/1‑295 (MQ=255) aCTAAATATTAATTAATTACTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTCCGGTAACCGCgctgccca > 2:223797/1‑292 (MQ=255) cTAAATATTAATTAATTACTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTCCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGCGCTGGCTCCCG‑A‑C‑CCCCAC‑CCGCACGCTaacgcgccggctcca > 2:73237/1‑282 (MQ=255) aaataattaattaCTGAGAAAAGTAGATGTGAAATAGCACCAACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAGGTTGATGTTAGAGGCAAAGGAACGATTCTGGTCGGGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGGGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTg < 2:367295/292‑1 (MQ=255) attaattaattaCTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTCCCCGAGCCGCTGCACGCttg > 2:888502/1‑293 (MQ=255) ttaattaattaCTGAGATATATAGATGTGAATTATCCCCCGCCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGat < 1:657375/295‑1 (MQ=255) ttaatgaattaCTGAGAGATATAGATGTGAATAATCCCCCACCCGGTCAGGCAGGGGATAACGTTTTCGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGATGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGat < 1:425950/295‑1 (MQ=255) taattaCTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCg < 1:417407/295‑1 (MQ=255) taCTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGCTTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ACC‑CCCC‑C‑CCGTCCGGTAATCGAGCTGCTGCCCGCTGCTATCCGcgcg > 1:768642/1‑292 (MQ=255) gatataGGTGTGAATTACCCCCAACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTGGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGAc < 2:240904/294‑1 (MQ=255) tataGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTCCGGTAATCGAGCTGCTGACCGCTGATCCCCGCCCGCATCTGCCGc > 1:135954/1‑295 (MQ=255) tataGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TACCCA‑C‑CCGTACGGTAATCGAGCTACAGACCGCTGACCTCTGCCAgattctctcgc > 1:307836/1‑285 (MQ=255) taGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTTGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTCATCGCGCTGCTGACCGCTGATATCCGCCAGCATCTgcaccca > 1:583184/1‑289 (MQ=255) taGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATCTCCGCCCGCATCTGACGcac > 1:757060/1‑293 (MQ=255) gATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGt > 1:845027/1‑295 (MQ=255) tgtgAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTAc < 1:507199/295‑1 (MQ=255) tggaaaTTATCCCCGCCCCGGTCAGGCAGGGGATAACGTTTGCGCCATTAAGGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAGCGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTAc < 1:271989/291‑1 (MQ=255) gtgAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGT‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑AGC‑CCCC‑C‑CCGGACGGTGCTCGGCCTGCTGACCGCCTGTAACCGCCCGACTCAGCCGCCAGTACt > 2:325554/1‑295 (MQ=255) tgctcttccgagctggTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGGCGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACt < 1:623076/281‑1 (MQ=255) aTTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACt < 1:869215/295‑1 (MQ=255) aTTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCGGACCGCTGCTATCCGCCAGCATCTGCCGCCAGCACTCCCt > 2:723455/1‑295 (MQ=255) ttATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGTAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTa < 1:534146/295‑1 (MQ=255) ccACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCCC‑A‑TCCCCC‑C‑CCGTCCGGTAATCGCGCTGCTGACCCCTGATTTCGCCCATCATCTTACCCCAGTACCATCTACGcgccc > 2:725678/1‑295 (MQ=255) cccGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccgc < 1:172035/295‑1 (MQ=255) tCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGCCCGCCGCTATCCGCCAGCATCTGACGCCAGTACTCAACAAGCGCCCCGCCCCCa > 1:185687/1‑295 (MQ=255) ggcaggGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCACTc > 1:820067/1‑295 (MQ=255) ggggATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTCCTCACTACGCGCCCCGCCCCCAATCAACg > 1:896822/1‑295 (MQ=255) ggggATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACg > 1:654645/1‑295 (MQ=255) gggATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTCCGGTAATCGAGCTGCTGACCGCTGACATCCGCCAGCCTCCGACGCCCGTCCTCCCTCCGCGCCCCCCCCCCCACCAACgc > 2:552147/1‑294 (MQ=255) tAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTACTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTCCGGTACTCCAGCTCCTGACCGCCGACACCCACCACCATCTGCCGCCCGCACTC‑CCACCCCCCCCACCCCCaaacccactccacc > 2:448140/1‑283 (MQ=255) aaCGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGCTCGGTAATCGAGCTGCCGACCGCCGATATCCGCCAGCAACTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAAccgcacaa > 1:4301/1‑288 (MQ=255) cggttACGCCATTAATGGCAGGAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACtt < 1:472663/292‑1 (MQ=255) gTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACttt < 1:505382/295‑1 (MQ=255) tttACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg < 1:486551/295‑1 (MQ=255) ttACGCCATTAATGGCAGAATTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTTGCTGATAATCGGCATAACGCTGCGCATCGGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCTGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGc < 1:540114/295‑1 (MQ=255) aCGCCATTAATAGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCgg < 1:105969/295‑1 (MQ=255) cGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGt < 1:472739/295‑1 (MQ=255) ccATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTGCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGttt < 1:147692/295‑1 (MQ=255) gAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCCTCCGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGCCGCCAGTACTCACTACGGGCCCCGCCCCAAACCACCGTATCACTTTGCCGTTTCATCACGCAGGc > 1:764642/1‑295 (MQ=255) aaGTTGCGGATAGAGGCGACTGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCAGCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCGATTAGCGA‑TTTCTCTGTATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCa < 1:85782/295‑1 (MQ=255) tGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCCCCCCGCCCCCACTCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCa > 1:604020/1‑295 (MQ=255) cTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCTAGCTGCTGACCGCTGATATCCGCCCGCCTCTCACGCCCGTCACCACTACCAGCCACGCCCCCACTCCACGTCACCCTTCTCCGTTTCATAcccaccgccgtccac > 2:857444/1‑282 (MQ=255) tAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTTCTGCCCGCTGATATCCGCCCGCATCTGACGCCCGTACTCACTCCCCGCCCCGCCCCCAATCAACGTAACACTTTGCGTCTTACTACCGCAGGCATTCCCGACAt > 1:549198/1‑295 (MQ=255) aggggcagcGGAACGTTTCGCGTCGTGGCGGAGAAGCGGCTTAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCCCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATc < 2:366027/287‑1 (MQ=255) gggCGACGGAGCGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCa < 1:152792/294‑1 (MQ=255) agGCGACGGAGCGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCa < 1:110246/295‑1 (MQ=255) ggCGTCGGAACGTTTCTCGTCGTTGCGGATAAGCGGCATAACGCTGTGCATTTGGTAGATGCGACTTTGCTAACGGTAGTTGCGGCAAGAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCAt < 2:676266/295‑1 (MQ=255) ggCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCAt > 1:554762/1‑295 (MQ=255) ggtaCGGAAGTTTTCTCGTTGTGACTAATAAGCGGTATAACGCGGCGCATCGGGTAGATGCGACTGTTCTAACGGTAGTTGCGGAAAAAAGGCAATGAGCGA‑TGTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATg < 2:881138/292‑1 (MQ=255) tgtcGGAACGTTTATCGTCGTGGCTGTGGAGCGGCATAACGCCGCGAATCGGGTAGATGGAACTGTGTGAACGGTAGTTGCGGCAACAAGTCAAGGTGGGA‑GTTCGCGGGAGTCGCCGCGATCTGCGCCATCCTTGCTGCGCCCAGGGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGc < 2:757060/292‑1 (MQ=255) gACGTAACGTTTCTCGTCGTGGCTGATAATCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGcc < 1:828059/295‑1 (MQ=255) cGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCAt < 1:594647/295‑1 (MQ=255) gtttccgtcgtGGCTGTTAAGCGTCATAACGCTGCGCAGCTGTTAGGTGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGT‑TTGTGCTGTATTCGCCGCGATGTGCGCCCTCCTGGCTGCGCTCAGTGCTGACCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGGGCTGCTTACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACTCGCCCCGCCCCCAATCAACGTGACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAg < 2:216753/290‑1 (MQ=255) tcgtGGCTGATAAGCGGCATAACGCTGCGCAACTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATaa < 1:201681/295‑1 (MQ=255) gATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑T‑CCCC‑CACCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCGAGCTTCTGACGCCAACCCACACTCCGCCCCCCGCCCCCAATCAACGCACCACTTTGCGGTTTACTACCGCACGCCGGAACAACATCCATCCCCTCCGCCAGCGCATaccacccgca > 2:619020/1‑286 (MQ=255) aaGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTc < 1:620380/295‑1 (MQ=255) cATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCAc < 1:213331/295‑1 (MQ=255) aTAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCCAGCTGCCGCCCGCTGATATCCGCCCGCCTCTTCCGCCAGTACTCCCTCCGCGCCCCGCCCCCCACCCACGTAACACCATGCGGTTTAATCCCGCAGGCACGCACGACCACCACGCCCCCGCCCACCGCCTACCCCACACCTTCCacccct > 2:117547/1‑290 (MQ=255) gcgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCCATCAACGTAACACTTTGCGGTTTACTACCGCGGGCCATCACCACACCACTGCCATCCTCCCGCGCCTCACCCCCCCCTCCCacccccccccgccc > 2:837768/1‑282 (MQ=255) tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAACAACGTAACACTTTGCGGTTTAATACCGCAGGAATGCACGACATCCATGCCCTCTGCCAGAGCATAACCCACGCCTTCCCGCACTGCTCCCTCCAGTTACTTGGGGc > 1:440621/1‑295 (MQ=255) cGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCa < 1:857444/295‑1 (MQ=255) gtggcgtgaCGGCAGGTGCGGCAACTATTTACTGA‑CGAGTTTGTCGGGTTTCGCCGGGAGCTGCGCCAGGCTTGCTGCGCCCAGTGCCGGCCCCCC‑C‑TACCCC‑C‑CCGTACGGTACGCGGGCTGCTGACCCCTGAGATCCGCCATCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCACTCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGt < 2:318189/287‑1 (MQ=255) cTGTTCGAACGGCAGGCGCGGCCACACTTCAGTCGGCGA‑GTTCCCGGGACCCGCGGCTGTCGCCGCCGTCCTTGCTGCGGCCAGTGCGGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGt < 2:658252/295‑1 (MQ=255) tCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTtgat < 1:223797/295‑1 (MQ=255) tAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTCCCAGCGCACAACCCACGCCTTCCCGCCCTGCTCGCGCCAGTTCATTGGGCCCATGTtgatga > 1:743442/1‑295 (MQ=255) tAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCCAGACATCCATGCCATCTGCCAGCGCATAACCCCCGCCTTCCAGCACCGATCGCCCCAGTTCATTGGGGCCATGTTGATGAGCCaaa > 1:774061/1‑295 (MQ=255) aacaaTTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCCCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGCCATCCATGCCATCTTCCAGCGCATACCCCCCCCCCTCCAGCCCCGACCGCCCCCGGTCATTCGTGCCATGCTGACGATTccaaccaacaaaca > 2:561355/1‑289 (MQ=255) acaaTTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCCCCCCCAATCAACGTAACACTTTGCGGTTTAATACCCCAGGCATGCACGACATCCATGCCATCTGCCAGCGCCTAACCCACGCCCTCCAGCCCCGCCCGCGCCGATTCATTAGGGCCATGTTGATGCGGCAAACCCACGAacac > 2:414886/1‑295 (MQ=255) caaTTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAAcc < 1:106507/295‑1 (MQ=255) aaTTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAAccc < 1:132670/295‑1 (MQ=255) tCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCACCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCt < 1:376011/295‑1 (MQ=255) aaTGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTc < 1:358970/295‑1 (MQ=255) cGA‑TTTCTCGGGATTCGCCGGGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTTCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTgg < 1:293432/295‑1 (MQ=255) gA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTggg < 1:384404/295‑1 (MQ=255) a‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCCGTTCATTGGCGCCATGTTGCTGAGTCGAACCAAAGAAAACCCCCTTCGCCTgggc > 1:191949/1‑294 (MQ=255) tttCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGa < 1:146804/295‑1 (MQ=255) tttCCCTGGATTCGCGGGGCTCTGCGCCAGCTTTGCTGGGCACAGGGCGGGCCCCCG‑AGC‑CCCC‑C‑CCGTACGGGAATCGGGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCGCTACGCGCCCCGCCCCCAATCAACGTAACGCTTTGCGGTTTAATCCCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGa < 2:506602/295‑1 (MQ=255) tgttcctgcgtgcgccgcGGTCTGCGCCAGCTTTGCGGCGCCCAGTGCGTGCCCCAC‑C‑TCCCCC‑C‑CCGTACGGGAATCGGGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGa < 2:185687/283‑1 (MQ=255) tctcTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCCTAACCCACGCCCTCCAGCACTGCCCGCCCCAGTTCCTTGGGGCACTGTTGATGAGCCACACCACAGCCAACCCCCTTCGCCTcgggctc > 2:327068/1‑292 (MQ=255) tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGCGACTATtg > 1:170667/1‑295 (MQ=255) tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATATGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCCGTTCATTGGGGCCCTGCTGATGAGTCATACCACAGCAAACCCGCTTCCCCCggcgcctcttg > 2:618802/1‑286 (MQ=255) tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCCGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCCCCCCGCCCCCCATCAACGTAACACTTTGCGGCTTAATACCGCAGGCCGGCCCGCCCTCCCTACCACCCGCCAGCCCCTAACCCACGCCTCCCCGCACTGCCCGCGCCAGCCCACTGCGGTCCTCTGGAGGCGCCACACCAAAGCAAACCCCCCTCGCCgggcacgacttg > 2:731057/1‑286 (MQ=255) cactCGCCGCGAGCGGGGCCAGCCTTGCGCGGCCCAGGGCGGGCCCACC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATtgtg < 2:135954/292‑1 (MQ=255) cgcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTa < 1:448140/295‑1 (MQ=255) cgcgcctggcCGGCCTGGCTGCGCCCACTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCGGACCGCTGATATCCCCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACg < 2:654645/287‑1 (MQ=255) gctcgtcgcCGGCCGTGCTGCGCCCAGGGCGGGCCCCAC‑C‑G‑CCCCAC‑CCGTACGGTAATCGGGCTGCTGACCGCTGAGATCCGCCAGCATCTGACGCCAGGACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTGAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCGAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGc < 2:191949/289‑1 (MQ=255) aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCt < 1:201778/295‑1 (MQ=255) gcgcCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTTCCAGCGCATAACCCACGCCTTCCAGCACTGCCCCCCCCAGTTCATTGCGGCCATGTTGATGAGTCCAACCAAAAAAAAACCCCTTCGCCACGCGATTCTTGTGTGGCGTACGCTcccc > 1:724891/1‑295 (MQ=255) aGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑TCCCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATTCCATCTGCCAGCGCATAACCCACGCCTTCCAGCCATGCTCGCCCCAGTTCATTGGGGCCATGTTGCTGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTCTTGTTTGGCGTTCGCTCGCCggcac > 1:798497/1‑292 (MQ=255) cccAGTGCTGGCCCCACAA‑C‑CCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCTCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGaa < 1:799175/295‑1 (MQ=255) | AACAAATGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA‑TTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑A‑T‑CCCC‑C‑CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAA > NZ_LN832404/3724331‑3724898 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |